SSGCID: Target Page

NemeA.00139.a

Dephospho-CoA kinase (EC 2.7.1.24) (Dephosphocoenzyme A kinase)

CENTER ID:	NemeA.00139.a
ORGANISM:	Neisseria meningitidis
ASSOCIATED DISEASE:	bacterial meningitis, meningococcal septicaemia, gonorrhea
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NemeA.00139.a.B1.GE41450	full length		1	210

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|487.517.peg.2410
UniProt:	X5F9J5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTVWVGLTGG IGSGKSAAAQ CFADLGVPRI DADAAAHSLT ASDGIALPEI RRLFGDTVFD TQGLLRRDIL RKEIFASPSR KALLESVMLP LIFSEIKKQQ ETFTDAAYGI VEIPLLTEKR QFISLIRRVL TISAPLEKRI GRVMARSGLT RGEVAAVISH QASESERLLL ADDVLLNDGS LKSLREKTMR LHAFYSGIFA SKPTQGKHNG

NT Sequence

atgacggtat gggtcggact gaccggcgga atcggcagcg gcaaatcggc agccgcgcaa tgttttgccg atttgggcgt accgcgtatc gatgcggatg cggcggcgca ctcgctgacg gcttcagacg gcatcgccct gccggaaatc aggcggctgt tcggcgacac cgttttcgac acacagggtt tgttgcgacg cgacatattg cgtaaagaaa tctttgcctc cccatcgcga aaagccttgc tcgaatccgt gatgttgccg ctgattttct cagaaatcaa aaaacagcaa gaaaccttta ccgatgcggc ttacggcatt gtcgaaattc cgctgctgac ggaaaagcgt caatttatca gcctgatacg gcgtgtcctg accataagcg cacctttgga aaaacgtatc ggcagggtga tggcccgcag cgggctgacg cgcggcgagg tggctgccgt catcagccat caggcatccg aatccgaacg cctgctgctt gcagacgatg tgctgctcaa tgacggcagc ctcaaaagcc tgcgtgagaa aacaatgcgc ctgcacgcgt tttattcagg gattttcgcc tcaaaaccaa cacaaggaaa acacaatggc tga

Details for NemeA.00139.a.B1.GE41450

HARVESTED ON:	4/5/2017
SEQUENCED ON:	4/27/2017
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT VWVGLTGGIG SGKSAAAQCF ADLGVPRIDA DAAAHSLTAS DGIALPEIRR LFGDTVFDTQ GLLRRDILRK EVFASPSRKA LLESVMLPLI FSEIKKQQET FTDAAYGIVE IPLLTEKRQF ISLIRRVLTI SAPVEKRIGR VMARSGLTRG EVAAVISHQA SESERLLLAD DVLLNDGSLK SLREKTMRLH AFYSGIFASK PTQGKHNG

Validated NT Sequence

atggctcacc accaccacca ccatatgacg gtatgggtcg gactgaccgg cggaatcggc agcggcaaat cggcagccgc gcaatgtttt gccgatttgg gcgtgccgcg catcgatgca gacgcggcgg cgcactcgct gacggcttca gacggcatcg ccctgccgga aatcaggcgg ctgttcggcg acaccgtttt tgacacacag ggtttgttgc ggcgcgacat attgcgtaaa gaagtctttg cctccccatc gcgaaaagcc ttgctcgaat ccgtgatgtt gccgctgatt ttctcagaaa tcaaaaaaca gcaagaaacc tttactgatg cagcttacgg cattgtcgaa attccgctgc tgacggaaaa gcgtcaattt atcagcctga tacggcgtgt cctgaccata agtgcccctg tggaaaaacg tatcggcagg gtgatggccc gcagcgggct gacgcgcggc gaggtggcgg ccgtcatcag ccatcaggca tccgaatccg aacgcctgct gcttgcagac gatgtgctgc tcaatgacgg cagcctcaaa agcctgcgtg agaaaacaat gcgcctgcac gcgttttatt cagggatttt cgcctcaaaa ccaacacaag gaaaacacaa tggctaagtg agtaagat

Expected Protein Sequence

MAHHHHHHMT VWVGLTGGIG SGKSAAAQCF ADLGVPRIDA DAAAHSLTAS DGIALPEIRR LFGDTVFDTQ GLLRRDILRK EIFASPSRKA LLESVMLPLI FSEIKKQQET FTDAAYGIVE IPLLTEKRQF ISLIRRVLTI SAPLEKRIGR VMARSGLTRG EVAAVISHQA SESERLLLAD DVLLNDGSLK SLREKTMRLH AFYSGIFASK PTQGKHNG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg tatgggtcgg actgaccggc ggaatcggca gcggcaaatc ggcagccgcg caatgttttg ccgatttggg cgtaccgcgt atcgatgcgg atgcggcggc gcactcgctg acggcttcag acggcatcgc cctgccggaa atcaggcggc tgttcggcga caccgttttc gacacacagg gtttgttgcg acgcgacata ttgcgtaaag aaatctttgc ctccccatcg cgaaaagcct tgctcgaatc cgtgatgttg ccgctgattt tctcagaaat caaaaaacag caagaaacct ttaccgatgc ggcttacggc attgtcgaaa ttccgctgct gacggaaaag cgtcaattta tcagcctgat acggcgtgtc ctgaccataa gcgcaccttt ggaaaaacgt atcggcaggg tgatggcccg cagcgggctg acgcgcggcg aggtggctgc cgtcatcagc catcaggcat ccgaatccga acgcctgctg cttgcagacg atgtgctgct caatgacggc agcctcaaaa gcctgcgtga gaaaacaatg cgcctgcacg cgttttattc agggattttc gcctcaaaac caacacaagg aaaacacaat ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat