NegoA.19241.a

penicillin-binding protein 1A

CENTER ID: NegoA.19241.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.19241.a.B2.GE40408 soluble domain 33 798
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.160
UniProt: B4RQ28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKKILTTCF GLFFGFCVFG VGLVAIAILV TYPKLPSLDS LQHYQPKMPL TIYSADGEVI GMYGEQRREF TKIGDFPEVL RNAVIAAEDK RFYRHWGVDV WGVARAAVGN VVSGSVQSGA STITQQVAKN FYLSSEKTFT RKFNEVLLAY KIEQSLSKDK ILELYFNQIY LGQRAYGFAS AAQIYFNKNV RDLTLAEAAM LAGLPKAPSA YNPIVNPERA KLRQKYILNN MLEEKMITVQ QRDQALNEEL HYERFVRKID QSALYVAEMV RRELYEKYGE DAYTQGFKVY TTVRTDHQKA ATEALRKALR NFDRGSSYRG AENYIDLSKS EDVEETVSQY LSGLYTVDKM VPAVVLDVTK KKNVVIQLPG GRRVALDRRA LGFAARAVDN EKMGEDRIRR GAVIRVKNNG GRWAVVQEPL PQGALVSLDA KTGAVRALVG GYDFHSKTFN RAVQAMRQPG STFKPFVYSA ALSKGMTAST VVNDAPISLP GKGPNGSVWT PKNSDGRYSG YITLRQALTA SKNMVSIRIL MSIGVGYAQQ YIRRFGFRPS ELPASLSMAL GTGETTPLKV AEAYSVFANG GYRVSSHVID KIYDRDGRLR AQMQPLVAGQ NAPQAIDPRN AYIMYKIMQD VVRVGTARGA AALGRTDIAG KTGTTNDNKD AWFVGFNPDV VTAVYIGFDK PKSMGRAGYG GTIAVPVWVD YMRFALKGKQ GKGMKMPEGV VSSNGEYYMK ERMVTDPGLM LDNSGIAPQP SRRAKEDDEA AVENEQQGRS DETRQDVQET PVLPSNTDSK QQQLDSLF
NT Sequence
ATGATTAAAA AGATTTTAAC TACTTGTTTT GGTTTGTTTT TTGGTTTTTG TGTATTTGGA GTGGGTCTGG TTGCCATTGC TATTTTGGTA ACGTATCCGA AACTGCCGTC TTTGGATTCT TTGCAGCATT ACCAGCCTAA AATGCCGTTG ACTATTTATT CGGCGGATGG AGAAGTCATC GGTATGTATG GGGAGCAGCG GCGCGAATTT ACAAAAATCG GCGATTTCCC CGAGGTGTTG CGGAATGCGG TTATTGCCGC CGAGGATAAA CGCTTTTACC GGCATTGGGG GGTGGATGTT TGGGGTGTTG CCCGCGCTGC CGTCGGCAAT GTCGTGTCCG GCAGCGTGCA GTCGGGTGCG AGTACGATTA CACAGCAGGT GGCGAAAAAT TTTTATTTGA GCAGTGAAAA AACGTTCACA CGCAAATTCA ATGAGGTGTT GCTTGCCTAT AAAATCGAGC AGTCTTTAAG CAAAGACAAA ATCCTTGAGT TGTATTTCAA TCAGATTTAC CTCGGTCAGC GCGCCTATGG TTTTGCATCT GCCGCGCAAA TCTATTTCAA TAAGAATGTC CGAGATTTGA CTTTGGCGGA AGCCGCCATG CTTGCGGGAC TGCCCAAGGC TCCGTCTGCC TATAATCCGA TTGTTAATCC GGAGCGTGCC AAGTTGCGCC AGAAGTATAT TTTGAACAAT ATGCTCGAGG AGAAGATGAT TACCGTGCAA CAGCGCGATC AGGCATTGAA TGAGGAACTG CATTATGAGC GGTTTGTTCG GAAAATCGAT CAGAGTGCTT TATATGTGGC GGAAATGGTG CGTCGGGAAC TGTATGAGAA ATATGGTGAA GATGCCTATA CGCAGGGTTT TAAGGTTTAT ACCACGGTCC GCACCGATCA TCAGAAGGCG GCAACCGAGG CATTGCGCAA GGCTCTACGG AATTTCGATC GCGGCAGCAG CTACCGCGGT GCGGAAAACT ATATCGATTT GAGTAAGAGT GAAGATGTCG AGGAGACTGT CAGCCAGTAT CTGTCGGGAC TCTATACCGT CGATAAAATG GTTCCCGCCG TTGTGTTGGA TGTTACTAAA AAGAAAAATG TCGTCATACA GCTGCCCGGC GGCAGGCGGG TTGCGCTTGA CAGGCGCGCC TTGGGTTTTG CGGCCCGAGC GGTCGATAAT GAGAAAATGG GGGAGGACCG TATCCGCAGG GGCGCGGTCA TCCGTGTCAA AAACAACGGC GGGCGTTGGG CGGTGGTTCA AGAGCCGTTG CCGCAGGGGG CTTTGGTTTC GCTGGATGCA AAAACCGGAG CTGTGCGCGC GCTGGTCGGC GGTTATGATT TTCACAGCAA AACATTCAAT CGTGCCGTTC AGGCAATGCG GCAGCCGGGT TCGACCTTTA AGCCGTTTGT CTATTCGGCG GCATTATCTA AGGGGATGAC CGCGTCCACA GTGGTTAACG ATGCGCCGAT TTCCCTGCCG GGGAAAGGGC CGAACGGTTC GGTTTGGACA CCTAAAAATT CAGACGGCAG ATATTCCGGC TACATTACTT TGAGACAGGC TCTGACGGCT TCCAAGAATA TGGTTTCCAT CCGTATTTTG ATGTCTATCG GTGTCGGTTA CGCGCAACAG TATATCCGGC GTTTCGGCTT CAGGCCGTCC GAGCTGCCGG CAAGCCTGTC TATGGCTTTA GGTACGGGCG AGACGACGCC GTTGAAAGTG GCGGAGGCAT ATAGTGTATT TGCGAACGGC GGATATAGGG TTTCTTCGCA CGTGATCGAT AAGATTTATG ACAGAGACGG CAGGTTGCGC GCCCAAATGC AACCTTTGGT GGCAGGGCAA AATGCGCCTC AGGCAATCGA TCCGCGCAAT GCCTATATTA TGTATAAGAT TATGCAGGAT GTGGTCCGTG TCGGTACGGC AAGGGGGGCA GCTGCGTTGG GAAGAACGGA TATTGCCGGT AAAACGGGTA CGACCAACGA CAATAAAGAT GCGTGGTTTG TCGGTTTTAA CCCTGATGTG GTTACTGCCG TATATATCGG CTTCGACAAA CCTAAGAGTA TGGGGCGTGC CGGCTACGGC GGTACGATTG CGGTGCCGGT TTGGGTGGAC TATATGCGTT TTGCGTTGAA AGGAAAGCAG GGCAAAGGGA TGAAAATGCC TGAAGGTGTG GTCAGCAGCA ATGGCGAATA CTATATGAAG GAACGTATGG TAACCGATCC GGGCTTGATG CTGGACAACA GCGGTATTGC GCCGCAACCT TCCCGACGGG CAAAAGAAGA TGATGAAGCG GCAGTAGAAA ACGAACAGCA GGGAAGGTCT GACGAAACGC GTCAGGACGT ACAGGAAACG CCGGTGCTTC CGAGCAATAC GGATTCCAAA CAGCAGCAGT TGGATTCCCT GTTTTAA
Details for NegoA.19241.a.B2.GE40408
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 86kDa
OBSERVED MW: 86kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHPK LPSLDSLQHY QPKMPLTIYS ADGEVIGMYG EQRREFTKIG DFPEVLRNAV IAAEDKRFYR HWGVDVWGVA RAAVGNVVSG SVQSGASTIT QQVAKNFYLS SEKTFTRKFN EVLLAYKIEQ SLSKDKILEL YFNQIYLGQR AYGFASAAQI YFNKNVRDLT LAEAAMLAGL PKAPSAYNPI VNPERAKLRQ KYILNNMLEE KMITVQQRDQ ALNEELHYER FVRKIDQSAL YVAEMVRREL YEKYGEDAYT QGFKVYTTVR TDHQKAATEA LRKALRNFDR GSSYRGAENY IDLSKSEDVE ETVSQYLSGL YTVDKMVPAV VLDVTKKKNV VIQLPGGRRV ALDRRALGFA ARAVDNEKMG EDRIRRGAVI RVKNNGGRWA VVQEPLPQGA LVSLDAKTGA VRALVGGYDF HSKTFNRAVQ AMRQPGSTFK PFVYSAALSK GMTASTVVND APISLPGKGP NGSVWTPKNS DGRYSGYITL RQALTASKNM VSIRILMSIG VGYAQQYIRR FGFRPSELPA SLSMALGTGE TTPLKVAEAY SVFANGGYRV SSHVIDKIYD RDGRLRAQMQ PLVAGQNAPQ AIDPRNAYIM YKIMQDVVRV GTARGAAALG RTDIAGKTGT TNDNKDAWFV GFNPDVVTAV YIGFDKPKSM GRAGYGGTIA VPVWVDYMRF ALKGKQGKGM KMPEGVVSSN GEYYMKERMV TDPGLMLDNS GIAPQPSRRA KEDDEAAVEN EQQGRSDETR QDVQETPVLP SNTDSKQQQL DSLF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catccgaaac tgccgtcttt ggattctttg cagcattacc agcctaaaat gccgttgact atttattcgg cggatggaga agtcatcggt atgtatgggg agcagcggcg cgaatttaca aaaatcggcg atttccccga ggtgttgcgg aatgcggtta ttgccgccga ggataaacgc ttttaccggc attggggggt ggatgtttgg ggtgttgccc gcgctgccgt cggcaatgtc gtgtccggca gcgtgcagtc gggtgcgagt acgattacac agcaggtggc gaaaaatttt tatttgagca gtgaaaaaac gttcacacgc aaattcaatg aggtgttgct tgcctataaa atcgagcagt ctttaagcaa agacaaaatc cttgagttgt atttcaatca gatttacctc ggtcagcgcg cctatggttt tgcatctgcc gcgcaaatct atttcaataa gaatgtccga gatttgactt tggcggaagc cgccatgctt gcgggactgc ccaaggctcc gtctgcctat aatccgattg ttaatccgga gcgtgccaag ttgcgccaga agtatatttt gaacaatatg ctcgaggaga agatgattac cgtgcaacag cgcgatcagg cattgaatga ggaactgcat tatgagcggt ttgttcggaa aatcgatcag agtgctttat atgtggcgga aatggtgcgt cgggaactgt atgagaaata tggtgaagat gcctatacgc agggttttaa ggtttatacc acggtccgca ccgatcatca gaaggcggca accgaggcat tgcgcaaggc tctacggaat ttcgatcgcg gcagcagcta ccgcggtgcg gaaaactata tcgatttgag taagagtgaa gatgtcgagg agactgtcag ccagtatctg tcgggactct ataccgtcga taaaatggtt cccgccgttg tgttggatgt tactaaaaag aaaaatgtcg tcatacagct gcccggcggc aggcgggttg cgcttgacag gcgcgccttg ggttttgcgg cccgagcggt cgataatgag aaaatggggg aggaccgtat ccgcaggggc gcggtcatcc gtgtcaaaaa caacggcggg cgttgggcgg tggttcaaga gccgttgccg cagggggctt tggtttcgct ggatgcaaaa accggagctg tgcgcgcgct ggtcggcggt tatgattttc acagcaaaac attcaatcgt gccgttcagg caatgcggca gccgggttcg acctttaagc cgtttgtcta ttcggcggca ttatctaagg ggatgaccgc gtccacagtg gttaacgatg cgccgatttc cctgccgggg aaagggccga acggttcggt ttggacacct aaaaattcag acggcagata ttccggctac attactttga gacaggctct gacggcttcc aagaatatgg tttccatccg tattttgatg tctatcggtg tcggttacgc gcaacagtat atccggcgtt tcggcttcag gccgtccgag ctgccggcaa gcctgtctat ggctttaggt acgggcgaga cgacgccgtt gaaagtggcg gaggcatata gtgtatttgc gaacggcgga tatagggttt cttcgcacgt gatcgataag atttatgaca gagacggcag gttgcgcgcc caaatgcaac ctttggtggc agggcaaaat gcgcctcagg caatcgatcc gcgcaatgcc tatattatgt ataagattat gcaggatgtg gtccgtgtcg gtacggcaag gggggcagct gcgttgggaa gaacggatat tgccggtaaa acgggtacga ccaacgacaa taaagatgcg tggtttgtcg gttttaaccc tgatgtggtt actgccgtat atatcggctt cgacaaacct aagagtatgg ggcgtgccgg ctacggcggt acgattgcgg tgccggtttg ggtggactat atgcgttttg cgttgaaagg aaagcagggc aaagggatga aaatgcctga aggtgtggtc agcagcaatg gcgaatacta tatgaaggaa cgtatggtaa ccgatccggg cttgatgctg gacaacagcg gtattgcgcc gcaaccttcc cgacgggcaa aagaagatga tgaagcggca gtagaaaacg aacagcaggg aaggtctgac gaaacgcgtc aggacgtaca ggaaacgccg gtgcttccga gcaatacgga ttccaaacag cagcagttgg attccctgtt ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat