SSGCID: Target Page

NegoA.19232.a

Cell division protein ZipA

CENTER ID:	NegoA.19232.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.19232.a.B2.GE40407	mature protein		17	428

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.19232.a.B2.PS38115	mature protein		17	428

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.414
UniProt:	B4RJR0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIYIVLFLAA VLAVVAYNMY QENQYRKKVR DQFGHSDKDA LLNSKTSHVR DGKPSGGPVM MPKPQPAVKK PAKPQDSAMR NLQEQDAVYI AKQKQAKASP FKTEIETALE EIGIIGNSAH TVSEPQTGHS APKPADAPAK PVPVPQTPAK PLITLKELSK VELPWFDVRF DFISYIALTE AKELHALPRL SNRCRYQIVG CTMDDHFQIA EPIPGIRYQA FIVGIQAVSR NGLASQEELS AFNRQADAFA QSMGGQTLHT DLAAFIEVAS ALDAFCARVD QTIAIHLVSP TSISGVELRS AVTGVGFVLE DDGAFHYTDT SGSTMFSICS LNNEPFTNAL LDNQSYKGFS MLLDIPHSPA GEKTFDDLFM DLAVRLSGQL NLNLVNDKME EVSTQWLKDV RTYVLARQSE MLKVGIEPGG KTALRLFS

NT Sequence

ATGATTTACA TCGTACTGTT CCTCGCCGCC GTCCTCGCCG TTGTCGCCTA CAATATGTAT CAGGAAAACC AATACCGCAA AAAAGTGCGC GACCAGTTCG GACACTCCGA CAAAGATGCC CTGCTCAACA GCAAAACCAG CCATGTCCGC GACGGCAAAC CGTCCGGCGG GCCAGTCATG ATGCCGAAAC CCCAACCGGC GGTCAAAAAA CCGGCCAAAC CCCAAGACTC CGCCATGCGC AACCTGCAAG AACAGGATGC CGTCTACATC GCCAAGCAGA AACAGGCAAA AGCCTCCCCG TTCAAAACCG AAATCGAAAC CGCCTTGGAA GAAATCGGCA TTATCGGCAA CTCCGCCCAC ACCGTTTCCG AACCCCAAAC CGGACATTCC GCACCGAAAC CTGCCGACGC GCCGGCAAAA CCCGTTCCCG TTCCGCAAAC GCCGGCAAAA CCGCTGATTA CGCTCAAAGA GCTGTCGAAG GTCGAGCTGC CCTGGTTTGA CGTGCGCTTC GACTTCATCT CCTATATCGC GCTGACCGAA GCCAAAGAAC TGCACGCACT GCCGCGCCTT TCCAACCGCT GCCGCTACCA GATTGTCGGC TGCACCATGG ACGACCATTT CCAGATTGCC GAACCCATCC CGGGCATCCG CTATCAGGCA TTTATCGTGG GTATCCAGGC AGTCAGCCGC AACGGACTTG CCTCGCAGGA AGAACTCTCC GCATTCAACC GCCAGGCGGA CGCATTCGCA CAAAGCATGG GCGGTCAGAC GCTGCACACC GACCTTGCCG CCTTTATCGA AGTGGCTTCC GCACTGGACG CATTCTGCGC GCGCGTCGAC CAGACCATCG CCATCCATTT GGTTTCGCCG ACCAGCATCA GCGGCGTAGA ACTGCGTTCC GCCGTAACGG GCGTGGGTTT CGTTTTGGAA GACGACGGCG CGTTCCACTA TACCGACACG TCGGGCTCGA CCATGTTCTC CATCTGCTCG CTCAACAACG AGCCGTTTAC CAATGCCCTT TTGGACAACC AGTCCTACAA AGGCTTCAGT ATGCTGCTCG ACATCCCGCA CTCTCCGGCA GGCGAAAAAA CCTTCGACGA TTTGTTTATG GATTTGGCGG TACGCCTGTC CGGTCAGTTG AACCTGAATC TGGTCAACGA CAAAATGGAA GAAGTTTCGA CCCAATGGCT CAAAGACGTA CGCACTTATG TATTGGCGCG TCAGTCCGAG ATGCTCAAAG TCGGTATCGA ACCGGGCGGC AAAACCGCCC TGCGCCTGTT TTCATAA

Details for NegoA.19232.a.B2.GE40407

HARVESTED ON:	7/15/2016
SEQUENCED ON:	7/18/2016
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Validated AA Sequence

MYNMYQENQY RKKVRDQFGH SDKDALLNSK TSHVRDGKPS GGPVMMPKPQ PAVKKPAKPQ DSAMRNLQEQ DAVYIAKQKQ AKASPFKTEI ETALEEIGII GNSAHTVSEP QTGHSAPKPA DAPAKPVPVP QTPAKPLITL KELSKVELPW FDVRFDFISY IALTEAKELH ALPRLSNRCR YQIVGCTMDD HFQIAEPIPG IRYQAFIVGI QAVSRNGLAS QEELSAFNRQ ADAFAQSMGG QTLHTDLAAF IEVASALDAF CARVDQTIAI HLVSPTSISG VELRSAVTGV GFVLEDDGAF HYTDTSGSTM FSICSLNNEP FTNALLDNQS YKGFSMLLDI PHSPAGEKTF DDLFMDLAVR LSGQLNLNLV NDKMEEVSTQ WLKDVRTYVL ARQSEMLKVG IEPGG

Validated NT Sequence

ttgccgcccg gttcgatacc gactttgagc atctcggact gacgcgccaa tacataagtg cgtacgtctt tgagccattg ggtcgaaact tcttccattt tgtcgttgac cagattcagg ttcaactgac cggacaggcg taccgccaaa tccataaaca aatcgtcgaa ggttttttcg cctgccggag agtgcgggat gtcgagcagc atactgaagc ctttgtagga ctggttgtcc aaaagggcat tggtaaacgg ctcgttgttg agcgagcaga tggagaacat ggtcgagccc gacgtgtcgg tatagtggaa cgcgccgtcg tcttccaaaa cgaaacccac gcccgttacg gcggaacgca gttctacgcc gctgatgctg gtcggcgaaa ccaaatggat ggcgatggtc tggtcgacgc gcgcgcagaa tgcgtccagt gcggaagcca cttcgataaa ggcggcaagg tcggtgtgca gcgtctgacc gcccatgctt tgtgcgaatg cgtccgcctg gcggttgaat gcggagagtt cttcctgcga ggcaagtccg ttgcggctga ctgcctggat acccacgata aatgcctgat agcggatgcc cgggatgggt tcggcaatct ggaaatggtc gtccatggtg cagccgacaa tctggtagcg gcagcggttg gaaaggcgcg gcagtgcgtg cagttctttg gcttcggtca gcgcgatata ggagatgaag tcgaagcgca cgtcaaacca gggcagctcg accttcgaca gctctttgag cgtaatcagc ggttttgccg gcgtttgcgg aacgggaacg ggttttgccg gcgcgtcggc aggtttcggt gcggaatgtc cggtttgggg ttcggaaacg gtgtgggcgg agttgccgat aatgccgatt tcttccaagg cggtttcgat ttcggttttg aacggggagg cttttgcctg tttctgcttg gcgatgtaga cggcatcctg ttcttgcagg ttgcgcatgg cggagtcttg gggtttggcc ggttttttga ccgccggttg gggtttcggc atcatgactg gcccgccgga cggtttgccg tcgcggacat ggctggtttt gctgttgagc agggcatctt tgtcggagtg tccgaactgg tcgcgcactt ttttgcggta ttggttttcc tgatacatat tgtacatatg gtggtggtgg tggtgag

Expected Protein Sequence

MAHHHHHHYN MYQENQYRKK VRDQFGHSDK DALLNSKTSH VRDGKPSGGP VMMPKPQPAV KKPAKPQDSA MRNLQEQDAV YIAKQKQAKA SPFKTEIETA LEEIGIIGNS AHTVSEPQTG HSAPKPADAP AKPVPVPQTP AKPLITLKEL SKVELPWFDV RFDFISYIAL TEAKELHALP RLSNRCRYQI VGCTMDDHFQ IAEPIPGIRY QAFIVGIQAV SRNGLASQEE LSAFNRQADA FAQSMGGQTL HTDLAAFIEV ASALDAFCAR VDQTIAIHLV SPTSISGVEL RSAVTGVGFV LEDDGAFHYT DTSGSTMFSI CSLNNEPFTN ALLDNQSYKG FSMLLDIPHS PAGEKTFDDL FMDLAVRLSG QLNLNLVNDK MEEVSTQWLK DVRTYVLARQ SEMLKVGIEP GGKTALRLFS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattacaata tgtatcagga aaaccaatac cgcaaaaaag tgcgcgacca gttcggacac tccgacaaag atgccctgct caacagcaaa accagccatg tccgcgacgg caaaccgtcc ggcgggccag tcatgatgcc gaaaccccaa ccggcggtca aaaaaccggc caaaccccaa gactccgcca tgcgcaacct gcaagaacag gatgccgtct acatcgccaa gcagaaacag gcaaaagcct ccccgttcaa aaccgaaatc gaaaccgcct tggaagaaat cggcattatc ggcaactccg cccacaccgt ttccgaaccc caaaccggac attccgcacc gaaacctgcc gacgcgccgg caaaacccgt tcccgttccg caaacgccgg caaaaccgct gattacgctc aaagagctgt cgaaggtcga gctgccctgg tttgacgtgc gcttcgactt catctcctat atcgcgctga ccgaagccaa agaactgcac gcactgccgc gcctttccaa ccgctgccgc taccagattg tcggctgcac catggacgac catttccaga ttgccgaacc catcccgggc atccgctatc aggcatttat cgtgggtatc caggcagtca gccgcaacgg acttgcctcg caggaagaac tctccgcatt caaccgccag gcggacgcat tcgcacaaag catgggcggt cagacgctgc acaccgacct tgccgccttt atcgaagtgg cttccgcact ggacgcattc tgcgcgcgcg tcgaccagac catcgccatc catttggttt cgccgaccag catcagcggc gtagaactgc gttccgccgt aacgggcgtg ggtttcgttt tggaagacga cggcgcgttc cactataccg acacgtcggg ctcgaccatg ttctccatct gctcgctcaa caacgagccg tttaccaatg cccttttgga caaccagtcc tacaaaggct tcagtatgct gctcgacatc ccgcactctc cggcaggcga aaaaaccttc gacgatttgt ttatggattt ggcggtacgc ctgtccggtc agttgaacct gaatctggtc aacgacaaaa tggaagaagt ttcgacccaa tggctcaaag acgtacgcac ttatgtattg gcgcgtcagt ccgagatgct caaagtcggt atcgaaccgg gcggcaaaac cgccctgcgc ctgttttcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for NegoA.19232.a.B2.PS38115

PURIFICATION DATe:	11/10/2016
CONCENTRATION:	39.68mg/ml
OBSERVED MW:	48kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTACAATA TGTATCAGGA AAACCAATAC CGCAAAAAAG TGCGCGACCA GTTCGGACAC TCCGACAAAG ATGCCCTGCT CAACAGCAAA ACCAGCCATG TCCGCGACGG CAAACCGTCC GGCGGGCCAG TCATGATGCC GAAACCCCAA CCGGCGGTCA AAAAACCGGC CAAACCCCAA GACTCCGCCA TGCGCAACCT GCAAGAACAG GATGCCGTCT ACATCGCCAA GCAGAAACAG GCAAAAGCCT CCCCGTTCAA AACCGAAATC GAAACCGCCT TGGAAGAAAT CGGCATTATC GGCAACTCCG CCCACACCGT TTCCGAACCC CAAACCGGAC ATTCCGCACC GAAACCTGCC GACGCGCCGG CAAAACCCGT TCCCGTTCCG CAAACGCCGG CAAAACCGCT GATTACGCTC AAAGAGCTGT CGAAGGTCGA GCTGCCCTGG TTTGACGTGC GCTTCGACTT CATCTCCTAT ATCGCGCTGA CCGAAGCCAA AGAACTGCAC GCACTGCCGC GCCTTTCCAA CCGCTGCCGC TACCAGATTG TCGGCTGCAC CATGGACGAC CATTTCCAGA TTGCCGAACC CATCCCGGGC ATCCGCTATC AGGCATTTAT CGTGGGTATC CAGGCAGTCA GCCGCAACGG ACTTGCCTCG CAGGAAGAAC TCTCCGCATT CAACCGCCAG GCGGACGCAT TCGCACAAAG CATGGGCGGT CAGACGCTGC ACACCGACCT TGCCGCCTTT ATCGAAGTGG CTTCCGCACT GGACGCATTC TGCGCGCGCG TCGACCAGAC CATCGCCATC CATTTGGTTT CGCCGACCAG CATCAGCGGC GTAGAACTGC GTTCCGCCGT AACGGGCGTG GGTTTCGTTT TGGAAGACGA CGGCGCGTTC CACTATACCG ACACGTCGGG CTCGACCATG TTCTCCATCT GCTCGCTCAA CAACGAGCCG TTTACCAATG CCCTTTTGGA CAACCAGTCC TACAAAGGCT TCAGTATGCT GCTCGACATC CCGCACTCTC CGGCAGGCGA AAAAACCTTC GACGATTTGT TTATGGATTT GGCGGTACGC CTGTCCGGTC AGTTGAACCT GAATCTGGTC AACGACAAAA TGGAAGAAGT TTCGACCCAA TGGCTCAAAG ACGTACGCAC TTATGTATTG GCGCGTCAGT CCGAGATGCT CAAAGTCGGT ATCGAACCGG GCGGCAAAAC CGCCCTGCGC CTGTTTTCAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT