NegoA.19228.a

ribosomal large subunit pseudouridine synthase D

CENTER ID:	NegoA.19228.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.19228.a.B1.GE40252	full length		1	374

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.19228.a.B1.PS38031	full length		1	374

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_85057

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.468
UniProt:	B4RJW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQNTSFDNES DYSDDSDFAS ASGTENRIGL TVPPELAGGR LDAVLAKLLP DYSRSRLTSW IKEGAVIVND KPSQPKDKMI GGEQICVTVR PSEENLAFVP EPMDLDIVYE DDTVIVVNKP AGLVVHPAAG NWTGTLLNGL LAHCPELSQI PRAGIVHRLD KETSGLMVVA KTLPAQNSLV RQLQERTVKR IYRAVANGIV PFDGKIETQI GRDPHNRLKM AAVKFGGKPA VTHVKVLERY LAHSYIECSL GTGRTHQIRV HMREANHPLA GDPVYGNPRH PCGDTVKEAV KSLGARQALH AYRLSFTHPE SGETVSFEAP IPDDIYHLLS VLRLEAGLDS SLSNEEEWQD KFGADDDDDW NEDDYDVEVV YVRE

NT Sequence

ATGCAGAATA CTTCCTTTGA TAATGAATCC GATTATAGCG ACGATTCAGA CTTTGCGTCA GCTTCCGGAA CTGAAAACCG TATCGGTCTG ACCGTTCCGC CCGAGCTTGC CGGCGGGCGG TTGGATGCGG TGTTGGCGAA ACTTCTGCCC GACTACTCGC GCAGCCGCCT GACATCATGG ATTAAAGAAG GCGCGGTTAT TGTAAACGAT AAACCTTCGC AACCCAAAGA CAAAATGATA GGCGGCGAGC AAATTTGTGT AACCGTCCGT CCGAGTGAGG AAAATCTGGC GTTTGTTCCG GAGCCTATGG ATTTGGATAT TGTTTACGAA GACGATACCG TCATCGTCGT CAACAAACCG GCCGGACTGG TGGTGCATCC GGCGGCGGGC AACTGGACGG GGACGCTGCT CAACGGCCTG TTGGCGCATT GCCCCGAGCT GTCCCAAATT CCGCGCGCGG GCATCGTACA CCGTTTGGAC AAAGAAACCA GCGGGCTGAT GGTGGTTGCC AAAACCCTGC CGGCGCAAAA TTCCCTCGTG AGGCAGCTTC AAGAACGCAC GGTCAAACGC ATCTACCGCG CCGTCGCCAA CGGCATCGTC CCCTTCGACG GTAAAATCGA AACCCAAATC GGACGCGATC CGCACAACCG CCTGAAAATG GCAGCCGTCA AATTCGGCGG CAAGCCTGCC GTAACCCACG TCAAAGTGTT GGAACGCTAT CTTGCCCACA GCTACATCGA ATGCTCGCTC GGAACGGGCA GGACGCACCA AATCCGCGTC CATATGCGCG AGGCCAACCA TCCGCTTGCC GGCGACCCGG TTTACGGCAA CCCGCGCCAT CCGTGCGGCG ACACGGTGAA AGAAGCCGTT AAAAGTTTGG GCGCGCGTCA GGCGTTGCAC GCCTACCGCT TGAGTTTTAC CCATCCGGAA AGCGGCGAAA CCGTTTCGTT TGAGGCACCG ATTCCGGACG ACATATATCA TTTGTTGTCC GTCCTCCGTC TTGAAGCCGG TTTGGATTCG TCTTTGAGCA ATGAAGAGGA ATGGCAGGAC AAATTCGGCG CGGACGACGA TGACGATTGG AACGAAGACG ACTACGATGT CGAAGTGGTT TATGTAAGGG AGTGA

Details for NegoA.19228.a.B1.GE40252

HARVESTED ON:	5/9/2016
SEQUENCED ON:	5/23/2016
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ NTSFDNESDY SDDSDFASAS GTENRIGLTV PLELAGGRLD AVLAKLLPDY SRSRLTSWIK EGAVIVNDKP SQPKDKMIGG EQICVTVRPS EENLAFVPEP MDLDIVYEDD TVIVVNKPAG LVVHPAAGNW TGTLLNGLLA HCPELSQIPR AGIVHRLDKE TSGLMVVAKT LPAQNSLVRQ LQERTVKRIY RAVANGIVPF DGKIETQIGR DPHNRLKMAA VKFGGKPAVT HVKVLERYLA HSYIECSLGT GRTHQIRVHM REANHPLAGD PVYGNPRHPC GDTVKEAVKS LGARQALHAY RLSFTHPESG ETVSFEAPIP DDIYHLLSVL RLEAGLDSSL SNEEEWQDKF GADDDDDWNE DD

Validated NT Sequence

gtagtcgtct tcgttccaat cgtcatcgtc gtccgcgccg aatttgtcct gccattcctc ttcattgctc aaagacgaat ccaaaccggc ttcaagacgg aggacggaca acaaatgata tatgtcgtcc ggaatcggtg cctcaaacga aacggtttcg ccgctttccg gatgggtaaa actcaagcgg taggcgtgca acgcctgacg cgcgcccaaa cttttaacgg cttctttcac cgtgtcgccg cacggatggc gcgggttgcc gtaaaccggg tcgccggcaa gcggatggtt ggcctcgcgc atatggacgc ggatttggtg cgtcctgccc gttccgagcg agcattcgat gtagctgtgg gcaagatagc gttccaacac tttgacgtgg gttacggcag gcttgccgcc gaatttgacg gctgccattt tcaggcggtt gtgcggatcg cgtccgattt gggtttcgat tttaccgtcg aaggggacga tgccgttggc gacggcgcgg tagatgcgtt tgaccgtgcg ttcttgaagc tgcctcacga gggaattttg cgccggcagg gttttggcaa ccaccatcag cccgctggtt tctttgtcca aacggtgtac gatgcccgcg cgcggaattt gggacagctc ggggcaatgc gccaacaggc cgttgagcag cgtccccgtc cagttgcccg ccgccggatg caccaccagt ccggccggtt tgttgacgac gatgacggta tcgtcttcgt aaacaatatc caaatccata ggctccggaa caaacgccag attttcctca ctcggacgga cggttacaca aatttgctcg ccgcctatca ttttgtcttt gggttgcgaa ggtttatcgt ttacaataac cgcgccttct ttaatccatg atgtcaggcg gctgcgcgaa tagtcgggca gaagtttcgc caacaccgca tccaaccgcc cgccggcaag ctcgagcgga acggtcagac cgatacggtt ttcagttccg gaagctgacg caaagtctga atcgtcgcta taatcggatt cattatcaaa ggaagtattc tgcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMQ NTSFDNESDY SDDSDFASAS GTENRIGLTV PPELAGGRLD AVLAKLLPDY SRSRLTSWIK EGAVIVNDKP SQPKDKMIGG EQICVTVRPS EENLAFVPEP MDLDIVYEDD TVIVVNKPAG LVVHPAAGNW TGTLLNGLLA HCPELSQIPR AGIVHRLDKE TSGLMVVAKT LPAQNSLVRQ LQERTVKRIY RAVANGIVPF DGKIETQIGR DPHNRLKMAA VKFGGKPAVT HVKVLERYLA HSYIECSLGT GRTHQIRVHM REANHPLAGD PVYGNPRHPC GDTVKEAVKS LGARQALHAY RLSFTHPESG ETVSFEAPIP DDIYHLLSVL RLEAGLDSSL SNEEEWQDKF GADDDDDWNE DDYDVEVVYV RE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga atacttcctt tgataatgaa tccgattata gcgacgattc agactttgcg tcagcttccg gaactgaaaa ccgtatcggt ctgaccgttc cgcccgagct tgccggcggg cggttggatg cggtgttggc gaaacttctg cccgactact cgcgcagccg cctgacatca tggattaaag aaggcgcggt tattgtaaac gataaacctt cgcaacccaa agacaaaatg ataggcggcg agcaaatttg tgtaaccgtc cgtccgagtg aggaaaatct ggcgtttgtt ccggagccta tggatttgga tattgtttac gaagacgata ccgtcatcgt cgtcaacaaa ccggccggac tggtggtgca tccggcggcg ggcaactgga cggggacgct gctcaacggc ctgttggcgc attgccccga gctgtcccaa attccgcgcg cgggcatcgt acaccgtttg gacaaagaaa ccagcgggct gatggtggtt gccaaaaccc tgccggcgca aaattccctc gtgaggcagc ttcaagaacg cacggtcaaa cgcatctacc gcgccgtcgc caacggcatc gtccccttcg acggtaaaat cgaaacccaa atcggacgcg atccgcacaa ccgcctgaaa atggcagccg tcaaattcgg cggcaagcct gccgtaaccc acgtcaaagt gttggaacgc tatcttgccc acagctacat cgaatgctcg ctcggaacgg gcaggacgca ccaaatccgc gtccatatgc gcgaggccaa ccatccgctt gccggcgacc cggtttacgg caacccgcgc catccgtgcg gcgacacggt gaaagaagcc gttaaaagtt tgggcgcgcg tcaggcgttg cacgcctacc gcttgagttt tacccatccg gaaagcggcg aaaccgtttc gtttgaggca ccgattccgg acgacatata tcatttgttg tccgtcctcc gtcttgaagc cggtttggat tcgtctttga gcaatgaaga ggaatggcag gacaaattcg gcgcggacga cgatgacgat tggaacgaag acgactacga tgtcgaagtg gtttatgtaa gggagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for NegoA.19228.a.B1.PS38031

PURIFICATION DATe:	8/24/2016
CONCENTRATION:	32mg/ml
OBSERVED MW:	42kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAGA ATACTTCCTT TGATAATGAA TCCGATTATA GCGACGATTC AGACTTTGCG TCAGCTTCCG GAACTGAAAA CCGTATCGGT CTGACCGTTC CGCCCGAGCT TGCCGGCGGG CGGTTGGATG CGGTGTTGGC GAAACTTCTG CCCGACTACT CGCGCAGCCG CCTGACATCA TGGATTAAAG AAGGCGCGGT TATTGTAAAC GATAAACCTT CGCAACCCAA AGACAAAATG ATAGGCGGCG AGCAAATTTG TGTAACCGTC CGTCCGAGTG AGGAAAATCT GGCGTTTGTT CCGGAGCCTA TGGATTTGGA TATTGTTTAC GAAGACGATA CCGTCATCGT CGTCAACAAA CCGGCCGGAC TGGTGGTGCA TCCGGCGGCG GGCAACTGGA CGGGGACGCT GCTCAACGGC CTGTTGGCGC ATTGCCCCGA GCTGTCCCAA ATTCCGCGCG CGGGCATCGT ACACCGTTTG GACAAAGAAA CCAGCGGGCT GATGGTGGTT GCCAAAACCC TGCCGGCGCA AAATTCCCTC GTGAGGCAGC TTCAAGAACG CACGGTCAAA CGCATCTACC GCGCCGTCGC CAACGGCATC GTCCCCTTCG ACGGTAAAAT CGAAACCCAA ATCGGACGCG ATCCGCACAA CCGCCTGAAA ATGGCAGCCG TCAAATTCGG CGGCAAGCCT GCCGTAACCC ACGTCAAAGT GTTGGAACGC TATCTTGCCC ACAGCTACAT CGAATGCTCG CTCGGAACGG GCAGGACGCA CCAAATCCGC GTCCATATGC GCGAGGCCAA CCATCCGCTT GCCGGCGACC CGGTTTACGG CAACCCGCGC CATCCGTGCG GCGACACGGT GAAAGAAGCC GTTAAAAGTT TGGGCGCGCG TCAGGCGTTG CACGCCTACC GCTTGAGTTT TACCCATCCG GAAAGCGGCG AAACCGTTTC GTTTGAGGCA CCGATTCCGG ACGACATATA TCATTTGTTG TCCGTCCTCC GTCTTGAAGC CGGTTTGGAT TCGTCTTTGA GCAATGAAGA GGAATGGCAG GACAAATTCG GCGCGGACGA CGATGACGAT TGGAACGAAG ACGACTACGA TGTCGAAGTG GTTTATGTAA GGGAGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT