NegoA.19208.a

DNA methylase

CENTER ID: NegoA.19208.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.19208.a.B1.GE40170 full length 1 252
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.21
UniProt: B4RNT5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
RSCHLSKSFE KIVSDDLVLY NENSLNVMRK ILEKHPNGCF DMIFADPPYF LSNDGFSCQN GQMVSVNKGN WDKSKGMAAD LEFYEEWLRL CYALLKPNGT IWVCGTFHNI YLIGYLMQTV GYHILNNITW EKPNPPPNLS CRFFTHSTET ILWAKKNKKA KHTFHYEMMK AQNNGKQMKC VWTFPPPNKT EKTFGKHPTQ KPLSLLERCI LSASNIGDLI FDPFMGSGTT GVAALKHGRR FCGCELEEDF LN
NT Sequence
agaagctgcc atttatcaaa gtcgtttgaa aaaatcgttt cagacgacct tgtcctctac aacgaaaatt cccttaacgt catgcggaag atattggaaa aacatccaaa cggctgtttt gatatgattt tcgcagaccc gccttacttt ctttccaatg acggtttcag ttgtcaaaac gggcaaatgg tttccgtcaa taagggcaac tgggataaat ccaaaggaat ggcggcagat ttggaatttt acgaagaatg gctccgactg tgttacgcct tattaaaacc aaacggcaca atttgggttt gcggcacatt tcataatatc tatttaatcg gctacctgat gcaaaccgtc ggctaccata ttttgaacaa tattacttgg gaaaaaccca atcctccccc taatttgtcc tgccgtttct ttacccattc gacagaaaca atcttatggg caaagaaaaa taaaaaagcc aaacatacgt ttcattatga aatgatgaaa gcacaaaata atggcaaaca gatgaaatgt gtttggacat ttcctccacc aaataaaacc gaaaaaacat tcggcaaaca tccgacacaa aaaccactct ccttacttga acgctgcata ctatcggctt caaatatcgg agatttaatt tttgaccctt ttatgggcag cggcacaaca ggcgttgccg ccttaaaaca tggtcggaga ttttgcggtt gcgaactgga agaagatttt ttgaattag
Details for NegoA.19208.a.B1.GE40170
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR SCHLSKSFEK IVSDDLVLYN ENSLNVMRKI LEKHPNGCFD MIFADPPYFL SNDGFSCQNG QMVSVNKGNW DKSKGMAADL EFYEEWLRLC YALLKPNGTI WVCGTFHNIY LIGYLMQTVG YHILNNITWE KPNPPPNLSC RFFTHSTETI LWAKKNKKAK HTFHYEMMKA QNNGKQMKCV WTFAPPNKTE KTFGKHPTQK PLPLLERCIL SASNIGDLIF DPFMGSGTTG VAALKHGRRF CGCELXEDFL N
Validated NT Sequence
atcttactca cttaattcaa aaaatcttct tncagttcgc aaccgcaaaa tctccgacca tgttttaagg cggcaacgcc tgttgtgccg ctgcccataa aagggtcaaa aattaaatct ccgatatttg aagccgatag tatgcagcgt tcaagtaagg ggagtggttt ttgtgtcgga tgtttgccga atgttttttc ggttttattt ggaggtgcga atgtccaaac acatttcatc tgtttgccat tattttgtgc tttcatcatt tcataatgaa acgtatgttt ggctttttta tttttctttg cccataagat tgtttctgtc gaatgggtaa agaaacggca ggacaaatta gggggaggat tgggtttttc ccaagtaata ttgttcaaaa tatggtagcc gacggtttgc atcaggtagc cgattaaata gatattatga aatgtgccgc aaacccaaat tgtgccgttt ggttttaata aggcgtaaca cagtcggagc cattcttcgt aaaattccaa atctgccgcc attcctttgg atttatccca gttgccctta ttgacggaaa ccatttgccc gttttgacaa ctgaaaccgt cattggaaag aaagtaaggc gggtctgcga aaatcatatc aaaacagccg tttggatgtt tttccaatat cttccgcatg acgttaaggg aattttcgtt gtagaggaca aggtcgtctg aaacgatttt ttcaaacgac tttgataaat ggcagcttct catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHRS CHLSKSFEKI VSDDLVLYNE NSLNVMRKIL EKHPNGCFDM IFADPPYFLS NDGFSCQNGQ MVSVNKGNWD KSKGMAADLE FYEEWLRLCY ALLKPNGTIW VCGTFHNIYL IGYLMQTVGY HILNNITWEK PNPPPNLSCR FFTHSTETIL WAKKNKKAKH TFHYEMMKAQ NNGKQMKCVW TFPPPNKTEK TFGKHPTQKP LSLLERCILS ASNIGDLIFD PFMGSGTTGV AALKHGRRFC GCELEEDFLN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagaagct gccatttatc aaagtcgttt gaaaaaatcg tttcagacga ccttgtcctc tacaacgaaa attcccttaa cgtcatgcgg aagatattgg aaaaacatcc aaacggctgt tttgatatga ttttcgcaga cccgccttac tttctttcca atgacggttt cagttgtcaa aacgggcaaa tggtttccgt caataagggc aactgggata aatccaaagg aatggcggca gatttggaat tttacgaaga atggctccga ctgtgttacg ccttattaaa accaaacggc acaatttggg tttgcggcac atttcataat atctatttaa tcggctacct gatgcaaacc gtcggctacc atattttgaa caatattact tgggaaaaac ccaatcctcc ccctaatttg tcctgccgtt tctttaccca ttcgacagaa acaatcttat gggcaaagaa aaataaaaaa gccaaacata cgtttcatta tgaaatgatg aaagcacaaa ataatggcaa acagatgaaa tgtgtttgga catttcctcc accaaataaa accgaaaaaa cattcggcaa acatccgaca caaaaaccac tctccttact tgaacgctgc atactatcgg cttcaaatat cggagattta atttttgacc cttttatggg cagcggcaca acaggcgttg ccgccttaaa acatggtcgg agattttgcg gttgcgaact ggaagaagat tttttgaatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat