NegoA.18998.a

NADH oxidoreductase (quinone). F subunit

CENTER ID: NegoA.18998.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.18998.a.B1.GE40150 full length 1 433
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.2312
UniProt: B4RPH9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIYQSGVIF DQVDTANPDC WTLDEYVKRG GYTALRKILS ENISQTDVID EVKTSGLRGR GGAGFPTGLK WSFMPRSFPG EKYVVCNTDE GEPGTFKDRD IIMFNPHALI EGMIIAGYAM GAKAGYNYIH GEIFEGYQRF EAALEQARAA GFLGKNILGS DFEFELFAHH GYGAYICGEE TALLESLEGK KGQPRFKPPF PASFGLYGKP TTINNTETFS SVPFIIRDGG QAFADKGIPN AGGTKLFCIS GHVERPGNYE VPLGTPFAEV LKMAGGMRGG KKLKAVIPGG SSAPVLPADI MMQTNMDYDS ISKAGSMLGS GAIIVMDEDV CMVKALERLS YFYYDESCGQ CTPCREGTGW LYRIVRRIVE GKGRMEDLDL LDSVGNQMAG RTICALADAA VFPVRSFTKH FRDEFAHYIE HGGPMKEHKW GGW
NT Sequence
ATGGCTATTT ACCAATCAGG CGTGATTTTT GACCAAGTGG ATACCGCCAA TCCCGATTGC TGGACATTGG ACGAATACGT CAAACGCGGC GGCTATACCG CCCTGCGTAA AATCCTGTCC GAAAACATCT CGCAAACCGA TGTGATTGAC GAAGTCAAAA CCTCCGGCTT GCGCGGGCGC GGCGGTGCGG GCTTCCCGAC CGGTTTGAAA TGGAGCTTTA TGCCCCGTTC TTTCCCGGGC GAAAAATATG TGGTTTGCAA CACCGACGAA GGCGAGCCGG GTACATTTAA AGACCGCGAC ATCATCATGT TCAATCCTCA TGCCCTGATT GAAGGCATGA TCATTGCCGG TTACGCGATG GGCGCGAAAG CCGGCTACAA CTATATCCAC GGCGAAATTT TCGAAGGTTA TCAACGCTTT GAAGCCGCTT TGGAGCAGGC GCGTGCCGCA GGCTTTTTGG GTAAAAATAT TTTGGGTTCG GATTTTGAAT TCGAACTCTT CGCCCACCAC GGCTACGGCG CATATATTTG CGGCGAAGAA ACCGCATTGC TTGAATCGTT GGAAGGCAAA AAAGGCCAGC CGCGCTTCAA ACCGCCGTTC CCCGCCTCGT TCGGCCTGTA CGGCAAACCG ACCACCATCA ACAATACCGA AACGTTCTCC TCCGTTCCAT TCATTATCCG TGACGGCGGA CAGGCGTTTG CCGATAAAGG TATTCCGAAT GCAGGCGGTA CCAAATTATT TTGTATTTCC GGCCATGTCG AGCGTCCGGG CAACTATGAA GTGCCGTTGG GCACGCCGTT TGCCGAAGTC TTGAAAATGG CGGGCGGTAT GCGCGGCGGT AAAAAACTCA AAGCCGTTAT TCCCGGCGGT TCGTCCGCCC CCGTATTGCC TGCCGACATT ATGATGCAGA CCAATATGGA CTATGACTCG ATTTCCAAAG CCGGCTCGAT GCTCGGTTCC GGCGCGATTA TCGTGATGGA CGAAGACGTG TGCATGGTCA AAGCCCTCGA ACGCTTAAGC TATTTCTACT ACGACGAATC CTGCGGCCAA TGCACCCCCT GCCGCGAAGG TACCGGCTGG CTTTACCGCA TCGTCCGCCG CATCGTAGAA GGCAAAGGCC GCATGGAAGA CTTGGATTTG CTGGATTCTG TCGGCAACCA AATGGCAGGC CGCACCATCT GCGCCCTTGC CGATGCCGCC GTCTTCCCTG TCCGCAGCTT TACCAAGCAT TTCCGTGATG AGTTTGCGCA TTACATCGAA CACGGCGGGC CGATGAAAGA GCATAAGTGG GGAGGGTGGT AA
Details for NegoA.18998.a.B1.GE40150
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA IYQSGVIFXX XDTANPDCWT LDEYVKRGGY TALRKILSEN ISQTDVIDEV KTSGLRGRGG AGFPTGLKWS FMPRSFPGEK YVVCNTDEGE PGTFKDRDII MFNPHALIEG MIIAGYAMGA KAGYNYIHGE IFEGYQRFEA ALEQARAAGF LGKNILGSDF EFELFAHHGY GAYICGEETA LLESLEGKKG QPRFKPPFPA SFGLYGKPTT INNTETFSSV PFIIRDGGQA FADKGIPNAG GTKLFCISGH VERPGNYEVP LGTPFAEVLK MAGGMRGGKK LKAVIPGGSS APVLPADIMM QTNMDYDSIS KAGSMLGSGA IIVMDEDVCM VKALERLSYF YYDESCGQCT PCREGTGWLY RIVRRIVEGK GRMEDLDLLD SVGNQMAGRT ICALADAAVF PVRSFTKHFR DEFAHYIEHG GPM
Validated NT Sequence
tttcatcggc ccgccgtgtt cgatgtaatg cgcaaactca tcacggaaat gcttggtaaa gctgcggaca gggaagacgg cggcatcggc aagggcgcag atggtgcggc ctgccatttg gttgccgaca gaatccagca aatccaagtc ttccatgcgg cctttgcctt ctacgatgcg gcggacgatg cggtaaagcc agccggtacc ttcgcggcag ggggtgcatt ggccgcagga ttcgtcgtag tagaaatagc ttaagcgttc gagggctttg accatgcaca cgtcttcgtc catcacgata atcgcgccgg aaccgagcat cgagccggct ttggaaatcg agtcatagtc catattggtc tgcatcataa tgtcggcagg caatacgggg gcggacgaac cgccgggaat aacggctttg agttttttac cgccgcgcat accgcccgcc attttcaaga cttcggcaaa cggcgtgccc aacggcactt catagttgcc cggacgctcg acatggccgg aaatacaaaa taatttggta ccgcctgcat tcggaatacc tttatcggca aacgcctgtc cgccgtcacg gataataaac gggacggagg agaacgtttc ggtattgttg atggtggtcg gtttgccgta caggccgaac gaggcgggga acggcggttt gaagcgcggc tggccttttt tgccttccaa cgattcaagc aatgcggttt cttcgccgca aatatatgcg ccgtagccgt ggtgggcgaa gagttcgaat tcaaaatccg aacccaaaat atttttaccc aaaaagcctg cggcacgcgc ctgctccaaa gcggcttcaa agcgttgata accttcgaaa atttcgccgt ggatatagtt gtagccggct ttcgcgccca tcgcgtaacc ggcaatgatc atgccttcaa tcagggcatg aggattgaac atgatgatgt cgcggtcttt aaatgtaccc ggctcgcctt cgtcggtgtt gcaaaccaca tatttttcgc ccgggaaaga acggggcata aagctccatt tcaaaccggt cgggaagccc gcaccgccgc gcccgcgcaa gccggaggtt ttgacttcgt caatcacatc ggtttgcgag atgttttcgg acaggatttt acgcagggcg gtatagccgc cgcgtttgac gtattcgtcc aatgtccagc aatcgggatt ggcggtatcc annnnnncaa aaatcacgcc tgattggtaa atagccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMA IYQSGVIFDQ VDTANPDCWT LDEYVKRGGY TALRKILSEN ISQTDVIDEV KTSGLRGRGG AGFPTGLKWS FMPRSFPGEK YVVCNTDEGE PGTFKDRDII MFNPHALIEG MIIAGYAMGA KAGYNYIHGE IFEGYQRFEA ALEQARAAGF LGKNILGSDF EFELFAHHGY GAYICGEETA LLESLEGKKG QPRFKPPFPA SFGLYGKPTT INNTETFSSV PFIIRDGGQA FADKGIPNAG GTKLFCISGH VERPGNYEVP LGTPFAEVLK MAGGMRGGKK LKAVIPGGSS APVLPADIMM QTNMDYDSIS KAGSMLGSGA IIVMDEDVCM VKALERLSYF YYDESCGQCT PCREGTGWLY RIVRRIVEGK GRMEDLDLLD SVGNQMAGRT ICALADAAVF PVRSFTKHFR DEFAHYIEHG GPMKEHKWGG W
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta tttaccaatc aggcgtgatt tttgaccaag tggataccgc caatcccgat tgctggacat tggacgaata cgtcaaacgc ggcggctata ccgccctgcg taaaatcctg tccgaaaaca tctcgcaaac cgatgtgatt gacgaagtca aaacctccgg cttgcgcggg cgcggcggtg cgggcttccc gaccggtttg aaatggagct ttatgccccg ttctttcccg ggcgaaaaat atgtggtttg caacaccgac gaaggcgagc cgggtacatt taaagaccgc gacatcatca tgttcaatcc tcatgccctg attgaaggca tgatcattgc cggttacgcg atgggcgcga aagccggcta caactatatc cacggcgaaa ttttcgaagg ttatcaacgc tttgaagccg ctttggagca ggcgcgtgcc gcaggctttt tgggtaaaaa tattttgggt tcggattttg aattcgaact cttcgcccac cacggctacg gcgcatatat ttgcggcgaa gaaaccgcat tgcttgaatc gttggaaggc aaaaaaggcc agccgcgctt caaaccgccg ttccccgcct cgttcggcct gtacggcaaa ccgaccacca tcaacaatac cgaaacgttc tcctccgttc cattcattat ccgtgacggc ggacaggcgt ttgccgataa aggtattccg aatgcaggcg gtaccaaatt attttgtatt tccggccatg tcgagcgtcc gggcaactat gaagtgccgt tgggcacgcc gtttgccgaa gtcttgaaaa tggcgggcgg tatgcgcggc ggtaaaaaac tcaaagccgt tattcccggc ggttcgtccg cccccgtatt gcctgccgac attatgatgc agaccaatat ggactatgac tcgatttcca aagccggctc gatgctcggt tccggcgcga ttatcgtgat ggacgaagac gtgtgcatgg tcaaagccct cgaacgctta agctatttct actacgacga atcctgcggc caatgcaccc cctgccgcga aggtaccggc tggctttacc gcatcgtccg ccgcatcgta gaaggcaaag gccgcatgga agacttggat ttgctggatt ctgtcggcaa ccaaatggca ggccgcacca tctgcgccct tgccgatgcc gccgtcttcc ctgtccgcag ctttaccaag catttccgtg atgagtttgc gcattacatc gaacacggcg ggccgatgaa agagcataag tggggagggt ggtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat