NegoA.18890.a

phenylalanyl-tRNA synthetase beta chain

CENTER ID: NegoA.18890.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.18890.a.AE1.GE43101 full length 1 787
NegoA.18890.a.B1.GE40148 full length 1 787
NegoA.18890.a.B11.GE42769 full length 1 787
NegoA.18890.a.B11.GE43247 full length 1 787
NegoA.18890.a.B12.GE42770 full length 1 787
NegoA.18890.a.B2.GE42620 truncation #1 1 786
NegoA.18890.a.B5.GE42623 truncation #4 5 781
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.18890.a.B1.PW37929 full length 1 787
NegoA.18890.a.B11.PW38732 full length 1 787
Molecular models
METHOD RESULTS
comparative modelling Robetta_85055
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|521006.8.peg.498
UniProt: B4RJZ3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQFSYSWLKT QANPDLSADK LEHLLTMAGL EVEEIDTAAP AFSGVVVAEV KSVEKHPDAD RLNVTQVDAG TGELVQIVCG APNVKPGIKV PCSLPVAVLP GNFKIKPTKM RGVPSNGMLC STNELGLPDD GVDGLHILPE DAPVGTNIRE YLDLDDMLFT LKITPNRADC LSVKGIAREV SALTQCAFTP VEIQTAPIGS EKKQAVRIDA PADCGRFISR VIENVNAKAA TPDWMKQRLE RSGIRSISAL VDIGNYVMLE IGQPMHVFDA DKLSGSLIVR RAQNGETLAC LNEKTVTLAD NTLVVADEKG ALSLAGLMGG AASAVSDGTQ NIVLEAAWFE PEIIVGKSRQ YGFGSDSSFR FERGVDYRLQ ADAIERATEL VLQICGGAAG EMVEAQGKLP EAKQVGLRLG RLKTVLGVDI PAEQVETILQ HLGLQPEKTA EGFRITAPSF RFDIEIEADL IEEIGRVYGY ENIPDDYTSG RLKMSELPET RRPRFAVYNE MAARGYREVI SYAFVDEQWE LDFAANAAPI RLQNPLAAQY AVMRSTLIGG LVEILQNNLN RKQNRVRVFE IACVFGKGSD GRFVQNERIG GLWYGAAMPE QWGEKTRNAD FYDIKADVEN LLKNKAVEFV KTGHPALHPG RAANIVSDGK VIGFVGELHP KWLQKYDLPQ APLVFEIDMA AVLECGKTRY RAVSKFQPVR RDLAFVMPEV MNHDDLLLVL KGAANKLVQE ISVFDVYRGT GLPEGMKSVA VKVILQDMEN TLTDEAVEPL IGKLIGVATE AGARLRS
NT Sequence
ATGCAATTCT CCTACTCATG GCTGAAAACC CAAGCCAATC CTGATCTTTC TGCCGATAAA CTGGAACATC TTTTGACCAT GGCCGGTTTG GAAGTGGAAG AAATCGATAC TGCCGCCCCT GCTTTCAGTG GCGTGGTTGT TGCCGAAGTA AAATCTGTTG AAAAACATCC TGATGCAGAC CGTTTGAACG TTACTCAAGT TGATGCCGGT ACGGGCGAGT TGGTTCAGAT TGTTTGTGGT GCGCCGAATG TCAAGCCGGG CATTAAAGTG CCGTGTTCGT TGCCGGTTGC CGTTTTGCCG GGCAATTTTA AAATTAAGCC GACTAAAATG CGCGGCGTAC CATCAAACGG TATGCTGTGT TCGACCAATG AACTGGGTTT GCCTGATGAT GGTGTAGACG GCCTGCACAT TCTGCCTGAA GATGCGCCTG TCGGTACCAA CATCCGCGAA TACTTGGATT TGGACGATAT GCTGTTTACG TTGAAAATTA CGCCTAACCG CGCTGATTGC TTGAGTGTTA AGGGCATTGC GCGTGAGGTT TCTGCGTTGA CTCAATGTGC GTTTACGCCT GTTGAAATTC AGACGGCCCC TATCGGCAGT GAGAAAAAAC AGGCTGTCCG TATTGATGCA CCGGCTGACT GCGGCCGTTT TATCAGCCGC GTTATTGAAA ATGTCAACGC GAAAGCGGCT ACTCCTGATT GGATGAAACA ACGTTTGGAA CGCAGCGGTA TCCGCAGCAT TTCCGCATTG GTGGACATTG GCAACTATGT GATGCTGGAA ATCGGTCAGC CTATGCATGT TTTCGATGCT GATAAGCTGT CGGGCAGTTT GATTGTCCGC CGTGCTCAAA ATGGCGAGAC TTTGGCGTGT CTGAATGAGA AGACGGTTAC TTTGGCTGAC AATACACTGG TGGTCGCTGA TGAAAAAGGT GCGTTGAGCT TGGCCGGCTT GATGGGTGGC GCGGCGAGCG CGGTTTCAGA CGGCACGCAA AATATCGTGC TGGAAGCGGC TTGGTTTGAG CCCGAAATCA TCGTCGGCAA ATCGCGCCAA TACGGTTTCG GTTCGGATTC GTCGTTCCGC TTCGAGCGCG GCGTGGATTA CCGTTTGCAG GCGGATGCCA TTGAACGCGC CACCGAATTG GTTTTGCAGA TTTGCGGCGG TGCGGCAGGC GAGATGGTGG AAGCACAAGG TAAATTGCCC GAAGCCAAGC AGGTCGGATT GCGTTTGGGT CGTCTGAAAA CCGTGTTGGG CGTGGACATT CCTGCCGAAC AGGTGGAAAC CATTTTGCAA CACTTGGGTC TGCAGCCTGA GAAAACGGCG GAAGGCTTCC GCATTACCGC GCCGAGCTTC CGTTTCGACA TCGAAATTGA GGCTGATTTG ATTGAAGAAA TCGGACGCGT TTACGGCTAT GAAAACATCC CCGACGATTA CACGTCAGGC CGTCTGAAAA TGTCGGAACT GCCCGAAACA CGCCGTCCGC GTTTTGCCGT TTACAACGAA ATGGCGGCGC GCGGCTACCG CGAAGTGATC AGCTATGCCT TCGTTGACGA GCAGTGGGAA CTCGATTTTG CCGCCAACGC CGCCCCCATC CGCCTGCAAA ACCCGCTGGC GGCGCAATAT GCCGTGATGC GTTCTACGTT GATTGGCGGC TTAGTGGAAA TTTTGCAAAA CAACCTGAAC CGCAAGCAAA ACCGCGTGCG CGTGTTTGAA ATCGCCTGCG TGTTCGGCAA AGGTTCAGAC GGCCGGTTTG TCCAAAACGA ACGCATCGGC GGTCTGTGGT ACGGCGCGGC GATGCCCGAA CAATGGGGTG AGAAAACGCG CAATGCGGAT TTTTACGACA TCAAGGCGGA CGTGGAAAAT CTGTTGAAAA ACAAAGCGGT CGAGTTCGTT AAAACCGGAC ATCCCGCCCT GCATCCCGGA CGTGCCGCCA ATATCGTTTC AGACGGCAAA GTCATCGGCT TTGTCGGCGA ACTGCATCCG AAATGGCTGC AAAAATACGA CCTGCCGCAA GCGCCGCTGG TATTTGAAAT CGATATGGCG GCTGTGTTGG AATGCGGGAA AACGCGCTAT CGGGCCGTAT CGAAATTCCA GCCGGTGCGC CGCGATTTGG CGTTTGTGAT GCCGGAAGTT ATGAATCATG ATGATTTACT GCTTGTCTTG AAAGGCGCAG CAAACAAGTT GGTACAGGAA ATCAGCGTGT TTGACGTGTA TCGCGGCACG GGTCTGCCCG AAGGGATGAA GAGCGTGGCG GTTAAAGTGA TTTTGCAGGA TATGGAAAAC ACGCTGACGG ATGAGGCGGT CGAGCCGCTT ATTGGAAAAC TGATTGGCGT GGCAACGGAG GCAGGGGCGC GGCTTCGCAG TTAA
Details for NegoA.18890.a.AE1.GE43101
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/25/2019
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 24
Validated AA Sequence
MPEQWGEKTR NADFYDIKAD VENLLKNKAV EFVKTGHPAL HPGRAANIVS DGKVIGFVGE LHPKWLQKYD LPQAPLVFEI DMAAVLECGK TRYRAVSKFQ PVRRDLAFVM PEVMNHDDLL LVLKGAANKL VQEISVFDVY RGTGLPEGMK SVAVKVILQD MENTLTDEAV EPLIGKLIGV ATEAGARLRS AGHHHHHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag cactgcgaag ccgcgcccct gcctccgttg ccacgccaat cagttttcca ataagcggct cgaccgcctc atccgtcagc gtgttttcca tatcctgcaa aatcacttta accgccacgc tcttcatccc ttcgggcaga cccgtgccgc gatacacgtc aaacacgctg atttcctgta ccaacttgtt tgctgcgcct ttcaagacaa gcagtaaatc atcatgattc ataacttccg gcatcacaaa cgccaaatcg cggcgcaccg gctggaattt cgatacggcc cgatagcgcg ttttcccgca ttccaacaca gccgccatat cgatttcaaa taccagcggc gcttgcggca ggtcgtattt ttgcagccat ttcggatgca gttcgccgac aaagccgatg actttgccgt ctgaaacgat attggcggca cgtccgggat gcagggcggg atgtccggtt ttaacgaact cgaccgcttt gtttttcaac agattttcca cgtccgcctt gatgtcgtaa aaatccgcat tgcgcgtttt ctcaccccat tgttcgggca tcgccgcgcc gtaccacaga ccgccgatgc gttcgttttg gacaaaccgg ccgtctgaac ctttgccgaa cacgcaggcg atttcaaaca cgcncacgcg gnnnttgctt gcggttcagg ttgttttgca aannnnccan nnnnccncca atnnnnnnnn nnnnnatcac ggcatatnng cgccgccagc nggtttnnnn nnnnnnnnng nnnngnnntn g
Expected Protein Sequence
MQFSYSWLKT QANPDLSADK LEHLLTMAGL EVEEIDTAAP AFSGVVVAEV KSVEKHPDAD RLNVTQVDAG TGELVQIVCG APNVKPGIKV PCSLPVAVLP GNFKIKPTKM RGVPSNGMLC STNELGLPDD GVDGLHILPE DAPVGTNIRE YLDLDDMLFT LKITPNRADC LSVKGIAREV SALTQCAFTP VEIQTAPIGS EKKQAVRIDA PADCGRFISR VIENVNAKAA TPDWMKQRLE RSGIRSISAL VDIGNYVMLE IGQPMHVFDA DKLSGSLIVR RAQNGETLAC LNEKTVTLAD NTLVVADEKG ALSLAGLMGG AASAVSDGTQ NIVLEAAWFE PEIIVGKSRQ YGFGSDSSFR FERGVDYRLQ ADAIERATEL VLQICGGAAG EMVEAQGKLP EAKQVGLRLG RLKTVLGVDI PAEQVETILQ HLGLQPEKTA EGFRITAPSF RFDIEIEADL IEEIGRVYGY ENIPDDYTSG RLKMSELPET RRPRFAVYNE MAARGYREVI SYAFVDEQWE LDFAANAAPI RLQNPLAAQY AVMRSTLIGG LVEILQNNLN RKQNRVRVFE IACVFGKGSD GRFVQNERIG GLWYGAAMPE QWGEKTRNAD FYDIKADVEN LLKNKAVEFV KTGHPALHPG RAANIVSDGK VIGFVGELHP KWLQKYDLPQ APLVFEIDMA AVLECGKTRY RAVSKFQPVR RDLAFVMPEV MNHDDLLLVL KGAANKLVQE ISVFDVYRGT GLPEGMKSVA VKVILQDMEN TLTDEAVEPL IGKLIGVATE AGARLRSGHH HHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gcaattctcc tactcatggc tgaaaaccca agccaatcct gatctttctg ccgataaact ggaacatctt ttgaccatgg ccggtttgga agtggaagaa atcgatactg ccgcccctgc tttcagtggc gtggttgttg ccgaagtaaa atctgttgaa aaacatcctg atgcagaccg tttgaacgtt actcaagttg atgccggtac gggcgagttg gttcagattg tttgtggtgc gccgaatgtc aagccgggca ttaaagtgcc gtgttcgttg ccggttgccg ttttgccggg caattttaaa attaagccga ctaaaatgcg cggcgtacca tcaaacggta tgctgtgttc gaccaatgaa ctgggtttgc ctgatgatgg tgtagacggc ctgcacattc tgcctgaaga tgcgcctgtc ggtaccaaca tccgcgaata cttggatttg gacgatatgc tgtttacgtt gaaaattacg cctaaccgcg ctgattgctt gagtgttaag ggcattgcgc gtgaggtttc tgcgttgact caatgtgcgt ttacgcctgt tgaaattcag acggccccta tcggcagtga gaaaaaacag gctgtccgta ttgatgcacc ggctgactgc ggccgtttta tcagccgcgt tattgaaaat gtcaacgcga aagcggctac tcctgattgg atgaaacaac gtttggaacg cagcggtatc cgcagcattt ccgcattggt ggacattggc aactatgtga tgctggaaat cggtcagcct atgcatgttt tcgatgctga taagctgtcg ggcagtttga ttgtccgccg tgctcaaaat ggcgagactt tggcgtgtct gaatgagaag acggttactt tggctgacaa tacactggtg gtcgctgatg aaaaaggtgc gttgagcttg gccggcttga tgggtggcgc ggcgagcgcg gtttcagacg gcacgcaaaa tatcgtgctg gaagcggctt ggtttgagcc cgaaatcatc gtcggcaaat cgcgccaata cggtttcggt tcggattcgt cgttccgctt cgagcgcggc gtggattacc gtttgcaggc ggatgccatt gaacgcgcca ccgaattggt tttgcagatt tgcggcggtg cggcaggcga gatggtggaa gcacaaggta aattgcccga agccaagcag gtcggattgc gtttgggtcg tctgaaaacc gtgttgggcg tggacattcc tgccgaacag gtggaaacca ttttgcaaca cttgggtctg cagcctgaga aaacggcgga aggcttccgc attaccgcgc cgagcttccg tttcgacatc gaaattgagg ctgatttgat tgaagaaatc ggacgcgttt acggctatga aaacatcccc gacgattaca cgtcaggccg tctgaaaatg tcggaactgc ccgaaacacg ccgtccgcgt tttgccgttt acaacgaaat ggcggcgcgc ggctaccgcg aagtgatcag ctatgccttc gttgacgagc agtgggaact cgattttgcc gccaacgccg cccccatccg cctgcaaaac ccgctggcgg cgcaatatgc cgtgatgcgt tctacgttga ttggcggctt agtggaaatt ttgcaaaaca acctgaaccg caagcaaaac cgcgtgcgcg tgtttgaaat cgcctgcgtg ttcggcaaag gttcagacgg ccggtttgtc caaaacgaac gcatcggcgg tctgtggtac ggcgcggcga tgcccgaaca atggggtgag aaaacgcgca atgcggattt ttacgacatc aaggcggacg tggaaaatct gttgaaaaac aaagcggtcg agttcgttaa aaccggacat cccgccctgc atcccggacg tgccgccaat atcgtttcag acggcaaagt catcggcttt gtcggcgaac tgcatccgaa atggctgcaa aaatacgacc tgccgcaagc gccgctggta tttgaaatcg atatggcggc tgtgttggaa tgcgggaaaa cgcgctatcg ggccgtatcg aaattccagc cggtgcgccg cgatttggcg tttgtgatgc cggaagttat gaatcatgat gatttactgc ttgtcttgaa aggcgcagca aacaagttgg tacaggaaat cagcgtgttt gacgtgtatc gcggcacggg tctgcccgaa gggatgaaga gcgtggcggt taaagtgatt ttgcaggata tggaaaacac gctgacggat gaggcggtcg agccgcttat tggaaaactg attggcgtgg caacggaggc aggggcgcgg cttcgcagtg ggcaccacca tcatcatcat taacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat
Details for NegoA.18890.a.B1.GE40148
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 87kDa
OBSERVED MW: 82kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN EKTVTLADNT LVVADEKGAL SLAGLMGGAA SAVSDGTQNI VLEAAWFEPE IIVGKSRQYG FGSDSSFRFE RGVDYRLQAD AIERATELVL QICGGAAGEM VEAQGKLPEA KQVGLRLGRL KTVLGVDIPA EQVETILQHL GLQPEKTAEG FRITAPSFRF DIEIEADLIE EIGRVYGYEN IPDDYTSGRL KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LECGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat tctcctactc atggctgaaa acccaagcca atcctgatct ttctgccgat aaactggaac atcttttgac catggccggt ttggaagtgg aagaaatcga tactgccgcc cctgctttca gtggcgtggt tgttgccgaa gtaaaatctg ttgaaaaaca tcctgatgca gaccgtttga acgttactca agttgatgcc ggtacgggcg agttggttca gattgtttgt ggtgcgccga atgtcaagcc gggcattaaa gtgccgtgtt cgttgccggt tgccgttttg ccgggcaatt ttaaaattaa gccgactaaa atgcgcggcg taccatcaaa cggtatgctg tgttcgacca atgaactggg tttgcctgat gatggtgtag acggcctgca cattctgcct gaagatgcgc ctgtcggtac caacatccgc gaatacttgg atttggacga tatgctgttt acgttgaaaa ttacgcctaa ccgcgctgat tgcttgagtg ttaagggcat tgcgcgtgag gtttctgcgt tgactcaatg tgcgtttacg cctgttgaaa ttcagacggc ccctatcggc agtgagaaaa aacaggctgt ccgtattgat gcaccggctg actgcggccg ttttatcagc cgcgttattg aaaatgtcaa cgcgaaagcg gctactcctg attggatgaa acaacgtttg gaacgcagcg gtatccgcag catttccgca ttggtggaca ttggcaacta tgtgatgctg gaaatcggtc agcctatgca tgttttcgat gctgataagc tgtcgggcag tttgattgtc cgccgtgctc aaaatggcga gactttggcg tgtctgaatg agaagacggt tactttggct gacaatacac tggtggtcgc tgatgaaaaa ggtgcgttga gcttggccgg cttgatgggt ggcgcggcga gcgcggtttc agacggcacg caaaatatcg tgctggaagc ggcttggttt gagcccgaaa tcatcgtcgg caaatcgcgc caatacggtt tcggttcgga ttcgtcgttc cgcttcgagc gcggcgtgga ttaccgtttg caggcggatg ccattgaacg cgccaccgaa ttggttttgc agatttgcgg cggtgcggca ggcgagatgg tggaagcaca aggtaaattg cccgaagcca agcaggtcgg attgcgtttg ggtcgtctga aaaccgtgtt gggcgtggac attcctgccg aacaggtgga aaccattttg caacacttgg gtctgcagcc tgagaaaacg gcggaaggct tccgcattac cgcgccgagc ttccgtttcg acatcgaaat tgaggctgat ttgattgaag aaatcggacg cgtttacggc tatgaaaaca tccccgacga ttacacgtca ggccgtctga aaatgtcgga actgcccgaa acacgccgtc cgcgttttgc cgtttacaac gaaatggcgg cgcgcggcta ccgcgaagtg atcagctatg ccttcgttga cgagcagtgg gaactcgatt ttgccgccaa cgccgccccc atccgcctgc aaaacccgct ggcggcgcaa tatgccgtga tgcgttctac gttgattggc ggcttagtgg aaattttgca aaacaacctg aaccgcaagc aaaaccgcgt gcgcgtgttt gaaatcgcct gcgtgttcgg caaaggttca gacggccggt ttgtccaaaa cgaacgcatc ggcggtctgt ggtacggcgc ggcgatgccc gaacaatggg gtgagaaaac gcgcaatgcg gatttttacg acatcaaggc ggacgtggaa aatctgttga aaaacaaagc ggtcgagttc gttaaaaccg gacatcccgc cctgcatccc ggacgtgccg ccaatatcgt ttcagacggc aaagtcatcg gctttgtcgg cgaactgcat ccgaaatggc tgcaaaaata cgacctgccg caagcgccgc tggtatttga aatcgatatg gcggctgtgt tggaatgcgg gaaaacgcgc tatcgggccg tatcgaaatt ccagccggtg cgccgcgatt tggcgtttgt gatgccggaa gttatgaatc atgatgattt actgcttgtc ttgaaaggcg cagcaaacaa gttggtacag gaaatcagcg tgtttgacgt gtatcgcggc acgggtctgc ccgaagggat gaagagcgtg gcggttaaag tgattttgca ggatatggaa aacacgctga cggatgaggc ggtcgagccg cttattggaa aactgattgg cgtggcaacg gaggcagggg cgcggcttcg cagttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for NegoA.18890.a.B11.GE42769
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 78
Validated AA Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN XKTXTLGXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XPDDYTSGRX KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LECGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLRS
Validated NT Sequence
atggctcacc accaccacca ccatatgcaa ttctcctact catggctgaa aacccaagcc aatcctgatc tttctgccga taaactggaa catcttttga ccatggccgg tttggaagtg gaagaaatcg atactgccgc ccctgctttc agtggcgtgg ttgttgccga agtaaaatct gttgaaaaac atcctgatgc agaccgtttg aacgttactc aagttgatgc cggtacgggc gagttggttc agattgtttg tggtgcgccg aatgtcaagc cgggcattaa agtgccgtgt tcgttgccgg ttgccgtttt gccgggcaat tttaaaatta agccgactaa aatgcgcggc gtaccatcaa acggtatgct gtgttcgacc aatgaactgg gtttgcctga tgatggtgta gacggcctgc acattctgcc tgaagatgcg cctgtcggta ccaacatccg cgaatacttg gatttggacg atatgctgtt tacgttgaaa attacgccta accgcgctga ttgcttgagt gttaagggca ttgcgcgtga ggtttctgcg ttgactcaat gtgcgtttac gcctgttgaa attcagacgg cccctatcgg cagtgagaaa aaacaggctg tccgtattga tgcaccggct gactgcggcc gttttatcag ccgcgttatt gaaaatgtca acgcgaaagc ggctactcct gattggatga aacaacgttt ggaacgcagc ggtatccgca gcatttccgc attggtggac attggcaact atgtgatgct ggaaatcggt cagcctatgc atgttttcga tgctgataag ctgtcgggca gtttgattgt ccgccgtgct caaaatggcg agactttggc gtgtctgaat ganaagacgg ntactttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccggacgatt acacgtcagg ccgtntgaaa atgtcggaac tgcccgaaac acgccgtccg cgttttgccg tttacaacga aatggcggcg cgcggctacc gcgaagtgat cagctatgcn ttcgttgacg agcagtggga actngatttt gccgccaacg ccgcccccat ccgcctgcaa aacccgctgg cggcgcaata tgccgtgatg cgttctacgt tgattggcgg nttagtggaa attttgcaaa acaacctgaa ccgcaagcaa aaccgcgtgc gcgtgtttga aatcgcctgc gtgttcggca aaggttcaga cggccggttt gtccaaaacg aacgcatcgg cggtctgtgg tacggcgcgg cgatgcccga acaatggggt gagaaaacgc gcaatgcgga tttttacgac atcaaggcgg acgtggaaaa tctgttgaaa aacaaagcgg tcgagttcgt taaaaccgga catcccgccc tgcatcccgg acgtgccgcc aatatcgttt cagacggcaa agtcatcggc tttgtcggcg aactgcatcc gaaatggctg caaaaatacg acctgccgca agcgccgctg gtatttgaaa tcgatatggc ggctgtgttg gaatgcggga aaacgcgcta tcgggccgta tcgaaattcc agccggtgcg ccgcgatttg gcgtttgtga tgccggaagt tatgaatcat gatgatttac tgcttgtctt gaaaggcgca gcaaacaagt tggtacagga aatcagcgtg tttgacgtgt atcgcggcac gggtctgccc gaagggatga agagcgtggc ggttaaagtg attttgcagg atatggaaaa cacgctgacg gatgaggcgg tcgagccgct tattggaaaa ctgattggcg tggcaacgga ggcaggggcg cggcttcgca gttaa
Expected Protein Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN EKTVTLADNT LVVADEKGAL SLAGLMGGAA SAVSDGTQNI VLEAAWFEPE IIVGKSRQYG FGSDSSFRFE RGVDYRLQAD AIERATELVL QICGGAAGEM VEAQGKLPEA KQVGLRLGRL KTVLGVDIPA EQVETILQHL GLQPEKTAEG FRITAPSFRF DIEIEADLIE EIGRVYGYEN IPDDYTSGRL KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LESGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat tct cctactcatg gctgaaaacc caagccaatc ctgatctttc tgccgataaa ctggaacatc ttttgaccat ggccggtttg gaagtggaag aaatcgatac tgccgcccct gctttcagtg gcgtggttgt tgccgaagta aaatctgttg aaaaacatcc tgatgcagac cgtttgaacg ttactcaagt tgatgccggt acgggcgagt tggttcagat tgtttgtggt gcgccgaatg tcaagccggg cattaaagtg ccgtgttcgt tgccggttgc cgttttgccg ggcaatttta aaattaagcc gactaaaatg cgcggcgtac catcaaacgg tatgctgtgt tcgaccaatg aactgggttt gcctgatgat ggtgtagacg gcctgcacat tctgcctgaa gatgcgcctg tcggtaccaa catccgcgaa tacttggatt tggacgatat gctgtttacg ttgaaaatta cgcctaaccg cgctgattgc ttgagtgtta agggcattgc gcgtgaggtt tctgcgttga ctcaatgtgc gtttacgcct gttgaaattc agacggcccc tatcggcagt gagaaaaaac aggctgtccg tattgatgca ccggctgact gcggccgttt tatcagccgc gttattgaaa atgtcaacgc gaaagcggct actcctgatt ggatgaaaca acgtttggaa cgcagcggta tccgcagcat ttccgcattg gtggacattg gcaactatgt gatgctggaa atcggtcagc ctatgcatgt tttcgatgct gataagctgt cgggcagttt gattgtccgc cgtgctcaaa atggcgagac tttggcgtgt ctgaatgaga agacggttac tttggctgac aatacactgg tggtcgctga tgaaaaaggt gcgttgagct tggccggctt gatgggtggc gcggcgagcg cggtttcaga cggcacgcaa aatatcgtgc tggaagcggc ttggtttgag cccgaaatca tcgtcggcaa atcgcgccaa tacggtttcg gttcggattc gtcgttccgc ttcgagcgcg gcgtggatta ccgtttgcag gcggatgcca ttgaacgcgc caccgaattg gttttgcaga tttgcggcgg tgcggcaggc gagatggtgg aagcacaagg taaattgccc gaagccaagc aggtcggatt gcgtttgggt cgtctgaaaa ccgtgttggg cgtggacatt cctgccgaac aggtggaaac cattttgcaa cacttgggtc tgcagcctga gaaaacggcg gaaggcttcc gcattaccgc gccgagcttc cgtttcgaca tcgaaattga ggctgatttg attgaagaaa tcggacgcgt ttacggctat gaaaacatcc ccgacgatta cacgtcaggc cgtctgaaaa tgtcggaact gcccgaaaca cgccgtccgc gttttgccgt ttacaacgaa atggcggcgc gcggctaccg cgaagtgatc agctatgcct tcgttgacga gcagtgggaa ctcgattttg ccgccaacgc cgcccccatc cgcctgcaaa acccgctggc ggcgcaatat gccgtgatgc gttctacgtt gattggcggc ttagtggaaa ttttgcaaaa caacctgaac cgcaagcaaa accgcgtgcg cgtgtttgaa atcgcctgcg tgttcggcaa aggttcagac ggccggtttg tccaaaacga acgcatcggc ggtctgtggt acggcgcggc gatgcccgaa caatggggtg agaaaacgcg caatgcggat ttttacgaca tcaaggcgga cgtggaaaat ctgttgaaaa acaaagcggt cgagttcgtt aaaaccggac atcccgccct gcatcccgga cgtgccgcca atatcgtttc agacggcaaa gtcatcggct ttgtcggcga actgcatccg aaatggctgc aaaaatacga cctgccgcaa gcgccgctgg tatttgaaat cgatatggcg gctgtgttgg aaagcgggaa aacgcgctat cgggccgtat cgaaattcca gccggtgcgc cgcgatttgg cgtttgtgat gccggaagtt atgaatcatg atgatttact gcttgtcttg aaaggcgcag caaacaagtt ggtacaggaa atcagcgtgt ttgacgtgta tcgcggcacg ggtctgcccg aagggatgaa gagcgtggcg gttaaagtga ttttgcagga tatggaaaac acgctgacgg atgaggcggt cgagccgctt attggaaaac tgattggcgt ggcaacggag gcaggggcgc ggcttcgcag ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for NegoA.18890.a.B11.GE43247
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 78
Validated AA Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN XKTXTLGXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XPDDYTSGRX KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LECGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLRS
Validated NT Sequence
atggctcacc accaccacca ccatatgcaa ttctcctact catggctgaa aacccaagcc aatcctgatc tttctgccga taaactggaa catcttttga ccatggccgg tttggaagtg gaagaaatcg atactgccgc ccctgctttc agtggcgtgg ttgttgccga agtaaaatct gttgaaaaac atcctgatgc agaccgtttg aacgttactc aagttgatgc cggtacgggc gagttggttc agattgtttg tggtgcgccg aatgtcaagc cgggcattaa agtgccgtgt tcgttgccgg ttgccgtttt gccgggcaat tttaaaatta agccgactaa aatgcgcggc gtaccatcaa acggtatgct gtgttcgacc aatgaactgg gtttgcctga tgatggtgta gacggcctgc acattctgcc tgaagatgcg cctgtcggta ccaacatccg cgaatacttg gatttggacg atatgctgtt tacgttgaaa attacgccta accgcgctga ttgcttgagt gttaagggca ttgcgcgtga ggtttctgcg ttgactcaat gtgcgtttac gcctgttgaa attcagacgg cccctatcgg cagtgagaaa aaacaggctg tccgtattga tgcaccggct gactgcggcc gttttatcag ccgcgttatt gaaaatgtca acgcgaaagc ggctactcct gattggatga aacaacgttt ggaacgcagc ggtatccgca gcatttccgc attggtggac attggcaact atgtgatgct ggaaatcggt cagcctatgc atgttttcga tgctgataag ctgtcgggca gtttgattgt ccgccgtgct caaaatggcg agactttggc gtgtctgaat ganaagacgg ntactttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccggacgatt acacgtcagg ccgtntgaaa atgtcggaac tgcccgaaac acgccgtccg cgttttgccg tttacaacga aatggcggcg cgcggctacc gcgaagtgat cagctatgcn ttcgttgacg agcagtggga actngatttt gccgccaacg ccgcccccat ccgcctgcaa aacccgctgg cggcgcaata tgccgtgatg cgttctacgt tgattggcgg nttagtggaa attttgcaaa acaacctgaa ccgcaagcaa aaccgcgtgc gcgtgtttga aatcgcctgc gtgttcggca aaggttcaga cggccggttt gtccaaaacg aacgcatcgg cggtctgtgg tacggcgcgg cgatgcccga acaatggggt gagaaaacgc gcaatgcgga tttttacgac atcaaggcgg acgtggaaaa tctgttgaaa aacaaagcgg tcgagttcgt taaaaccgga catcccgccc tgcatcccgg acgtgccgcc aatatcgttt cagacggcaa agtcatcggc tttgtcggcg aactgcatcc gaaatggctg caaaaatacg acctgccgca agcgccgctg gtatttgaaa tcgatatggc ggctgtgttg gaatgcggga aaacgcgcta tcgggccgta tcgaaattcc agccggtgcg ccgcgatttg gcgtttgtga tgccggaagt tatgaatcat gatgatttac tgcttgtctt gaaaggcgca gcaaacaagt tggtacagga aatcagcgtg tttgacgtgt atcgcggcac gggtctgccc gaagggatga agagcgtggc ggttaaagtg attttgcagg atatggaaaa cacgctgacg gatgaggcgg tcgagccgct tattggaaaa ctgattggcg tggcaacgga ggcaggggcg cggcttcgca gttaa
Expected Protein Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN EKTVTLADNT LVVADEKGAL SLAGLMGGAA SAVSDGTQNI VLEAAWFEPE IIVGKSRQYG FGSDSSFRFE RGVDYRLQAD AIERATELVL QICGGAAGEM VEAQGKLPEA KQVGLRLGRL KTVLGVDIPA EQVETILQHL GLQPEKTAEG FRITAPSFRF DIEIEADLIE EIGRVYGYEN IPDDYTSGRL KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LESGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat tct cctactcatg gctgaaaacc caagccaatc ctgatctttc tgccgataaa ctggaacatc ttttgaccat ggccggtttg gaagtggaag aaatcgatac tgccgcccct gctttcagtg gcgtggttgt tgccgaagta aaatctgttg aaaaacatcc tgatgcagac cgtttgaacg ttactcaagt tgatgccggt acgggcgagt tggttcagat tgtttgtggt gcgccgaatg tcaagccggg cattaaagtg ccgtgttcgt tgccggttgc cgttttgccg ggcaatttta aaattaagcc gactaaaatg cgcggcgtac catcaaacgg tatgctgtgt tcgaccaatg aactgggttt gcctgatgat ggtgtagacg gcctgcacat tctgcctgaa gatgcgcctg tcggtaccaa catccgcgaa tacttggatt tggacgatat gctgtttacg ttgaaaatta cgcctaaccg cgctgattgc ttgagtgtta agggcattgc gcgtgaggtt tctgcgttga ctcaatgtgc gtttacgcct gttgaaattc agacggcccc tatcggcagt gagaaaaaac aggctgtccg tattgatgca ccggctgact gcggccgttt tatcagccgc gttattgaaa atgtcaacgc gaaagcggct actcctgatt ggatgaaaca acgtttggaa cgcagcggta tccgcagcat ttccgcattg gtggacattg gcaactatgt gatgctggaa atcggtcagc ctatgcatgt tttcgatgct gataagctgt cgggcagttt gattgtccgc cgtgctcaaa atggcgagac tttggcgtgt ctgaatgaga agacggttac tttggctgac aatacactgg tggtcgctga tgaaaaaggt gcgttgagct tggccggctt gatgggtggc gcggcgagcg cggtttcaga cggcacgcaa aatatcgtgc tggaagcggc ttggtttgag cccgaaatca tcgtcggcaa atcgcgccaa tacggtttcg gttcggattc gtcgttccgc ttcgagcgcg gcgtggatta ccgtttgcag gcggatgcca ttgaacgcgc caccgaattg gttttgcaga tttgcggcgg tgcggcaggc gagatggtgg aagcacaagg taaattgccc gaagccaagc aggtcggatt gcgtttgggt cgtctgaaaa ccgtgttggg cgtggacatt cctgccgaac aggtggaaac cattttgcaa cacttgggtc tgcagcctga gaaaacggcg gaaggcttcc gcattaccgc gccgagcttc cgtttcgaca tcgaaattga ggctgatttg attgaagaaa tcggacgcgt ttacggctat gaaaacatcc ccgacgatta cacgtcaggc cgtctgaaaa tgtcggaact gcccgaaaca cgccgtccgc gttttgccgt ttacaacgaa atggcggcgc gcggctaccg cgaagtgatc agctatgcct tcgttgacga gcagtgggaa ctcgattttg ccgccaacgc cgcccccatc cgcctgcaaa acccgctggc ggcgcaatat gccgtgatgc gttctacgtt gattggcggc ttagtggaaa ttttgcaaaa caacctgaac cgcaagcaaa accgcgtgcg cgtgtttgaa atcgcctgcg tgttcggcaa aggttcagac ggccggtttg tccaaaacga acgcatcggc ggtctgtggt acggcgcggc gatgcccgaa caatggggtg agaaaacgcg caatgcggat ttttacgaca tcaaggcgga cgtggaaaat ctgttgaaaa acaaagcggt cgagttcgtt aaaaccggac atcccgccct gcatcccgga cgtgccgcca atatcgtttc agacggcaaa gtcatcggct ttgtcggcga actgcatccg aaatggctgc aaaaatacga cctgccgcaa gcgccgctgg tatttgaaat cgatatggcg gctgtgttgg aaagcgggaa aacgcgctat cgggccgtat cgaaattcca gccggtgcgc cgcgatttgg cgtttgtgat gccggaagtt atgaatcatg atgatttact gcttgtcttg aaaggcgcag caaacaagtt ggtacaggaa atcagcgtgt ttgacgtgta tcgcggcacg ggtctgcccg aagggatgaa gagcgtggcg gttaaagtga ttttgcagga tatggaaaac acgctgacgg atgaggcggt cgagccgctt attggaaaac tgattggcgt ggcaacggag gcaggggcgc ggcttcgcag ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for NegoA.18890.a.B12.GE42770
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 37
Validated AA Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHXFDADK LSGXLIVRRA QX
Validated NT Sequence
atggctcacc accaccacca ccatatgcaa ttctcctact catggctgaa aacccaagcc aatcctgatc tttctgccga taaactggaa catcttttga ccatggccgg tttggaagtg gaagaaatcg atactgccgc ccctgctttc agtggcgtgg ttgttgccga agtaaaatct gttgaaaaac atcctgatgc agaccgtttg aacgttactc aagttgatgc cggtacgggc gagttggttc agattgtttg tggtgcgccg aatgtcaagc cgggcattaa agtgccgtgt tcgttgccgg ttgccgtttt gccgggcaat tttaaaatta agccgactaa aatgcgcggc gtaccatcaa acggtatgct gtgttcgacc aatgaactgg gtttgcctga tgatggtgta gacggcctgc acattctgcc tgaagatgcg cctgtcggta ccaacatccg cgaatacttg gatttggacg atatgctgtt tacgttgaaa attacgccta accgcgctga ttgcttgagt gttaagggca ttgcgcgtga ggtttctgcg ttgactcaat gtgcgtttac gcctgttgaa attcagacgg cccctatcgg cagtgagaaa aaacaggctg tccgtattga tgcaccggct gactgcggcc gttttatcag ccgcgttatt gaaaatgtca acgcgaaagc ggctactcct gattggatga aacaacgttt ggaacgcagc ggtatccgca gcatttccgc attggtggac attggcaact atgtgatgct ggaaatcggt cagcctatgc atgntttcga tgctgataag ctgtcgggca nnttgattgt ccgccgtgct caantg
Expected Protein Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSAA AQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN EKTVTLADNT LVVADEKGAL SLAGLMGGAA SAVSDGTQNI VLEAAWFEPE IIVGKSRQYG FGSDSSFRFE RGVDYRLQAD AIERATELVL QICGGAAGEM VEAQGKLPEA KQVGLRLGRL KTVLGVDIPA EQVETILQHL GLQPEKTAEG FRITAPSFRF DIEIEADLIE EIGRVYGYEN IPDDYTSGRL KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LECGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLRS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat tct cctactcatg gctgaaaacc caagccaatc ctgatctttc tgccgataaa ctggaacatc ttttgaccat ggccggtttg gaagtggaag aaatcgatac tgccgcccct gctttcagtg gcgtggttgt tgccgaagta aaatctgttg aaaaacatcc tgatgcagac cgtttgaacg ttactcaagt tgatgccggt acgggcgagt tggttcagat tgtttgtggt gcgccgaatg tcaagccggg cattaaagtg ccgtgttcgt tgccggttgc cgttttgccg ggcaatttta aaattaagcc gactaaaatg cgcggcgtac catcaaacgg tatgctgtgt tcgaccaatg aactgggttt gcctgatgat ggtgtagacg gcctgcacat tctgcctgaa gatgcgcctg tcggtaccaa catccgcgaa tacttggatt tggacgatat gctgtttacg ttgaaaatta cgcctaaccg cgctgattgc ttgagtgtta agggcattgc gcgtgaggtt tctgcgttga ctcaatgtgc gtttacgcct gttgaaattc agacggcccc tatcggcagt gccgcagcac aggctgtccg tattgatgca ccggctgact gcggccgttt tatcagccgc gttattgaaa atgtcaacgc gaaagcggct actcctgatt ggatgaaaca acgtttggaa cgcagcggta tccgcagcat ttccgcattg gtggacattg gcaactatgt gatgctggaa atcggtcagc ctatgcatgt tttcgatgct gataagctgt cgggcagttt gattgtccgc cgtgctcaaa atggcgagac tttggcgtgt ctgaatgaga agacggttac tttggctgac aatacactgg tggtcgctga tgaaaaaggt gcgttgagct tggccggctt gatgggtggc gcggcgagcg cggtttcaga cggcacgcaa aatatcgtgc tggaagcggc ttggtttgag cccgaaatca tcgtcggcaa atcgcgccaa tacggtttcg gttcggattc gtcgttccgc ttcgagcgcg gcgtggatta ccgtttgcag gcggatgcca ttgaacgcgc caccgaattg gttttgcaga tttgcggcgg tgcggcaggc gagatggtgg aagcacaagg taaattgccc gaagccaagc aggtcggatt gcgtttgggt cgtctgaaaa ccgtgttggg cgtggacatt cctgccgaac aggtggaaac cattttgcaa cacttgggtc tgcagcctga gaaaacggcg gaaggcttcc gcattaccgc gccgagcttc cgtttcgaca tcgaaattga ggctgatttg attgaagaaa tcggacgcgt ttacggctat gaaaacatcc ccgacgatta cacgtcaggc cgtctgaaaa tgtcggaact gcccgaaaca cgccgtccgc gttttgccgt ttacaacgaa atggcggcgc gcggctaccg cgaagtgatc agctatgcct tcgttgacga gcagtgggaa ctcgattttg ccgccaacgc cgcccccatc cgcctgcaaa acccgctggc ggcgcaatat gccgtgatgc gttctacgtt gattggcggc ttagtggaaa ttttgcaaaa caacctgaac cgcaagcaaa accgcgtgcg cgtgtttgaa atcgcctgcg tgttcggcaa aggttcagac ggccggtttg tccaaaacga acgcatcggc ggtctgtggt acggcgcggc gatgcccgaa caatggggtg agaaaacgcg caatgcggat ttttacgaca tcaaggcgga cgtggaaaat ctgttgaaaa acaaagcggt cgagttcgtt aaaaccggac atcccgccct gcatcccgga cgtgccgcca atatcgtttc agacggcaaa gtcatcggct ttgtcggcga actgcatccg aaatggctgc aaaaatacga cctgccgcaa gcgccgctgg tatttgaaat cgatatggcg gctgtgttgg aatgcgggaa aacgcgctat cgggccgtat cgaaattcca gccggtgcgc cgcgatttgg cgtttgtgat gccggaagtt atgaatcatg atgatttact gcttgtcttg aaaggcgcag caaacaagtt ggtacaggaa atcagcgtgt ttgacgtgta tcgcggcacg ggtctgcccg aagggatgaa gagcgtggcg gttaaagtga ttttgcagga tatggaaaac acgctgacgg atgaggcggt cgagccgctt attggaaaac tgattggcgt ggcaacggag gcaggggcgc ggcttcgcag ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for NegoA.18890.a.B2.GE42620
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 87kDa
OBSERVED MW: 87kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN EKTVTLADNT LVVADEKGAL SLAGLMGGAA SAVSDGTQNI VLEAAWFEPE IIVGKSRQYG FGSDSSFRFE RGVDYRLQAD AIERATELVL QICGGAAGEM VEAQGKLPEA KQVGLRLGRL KTVLGVDIPA EQVETILQHL GLQPEKTAEG FRITAPSFRF DIEIEADLIE EIGRVYGYEN IPDDYTSGRL KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LECGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaat tctcctactc atggctgaaa acccaagcca atcctgatct ttctgccgat aaactggaac atcttttgac catggccggt ttggaagtgg aagaaatcga tactgccgcc cctgctttca gtggcgtggt tgttgccgaa gtaaaatctg ttgaaaaaca tcctgatgca gaccgtttga acgttactca agttgatgcc ggtacgggcg agttggttca gattgtttgt ggtgcgccga atgtcaagcc gggcattaaa gtgccgtgtt cgttgccggt tgccgttttg ccgggcaatt ttaaaattaa gccgactaaa atgcgcggcg taccatcaaa cggtatgctg tgttcgacca atgaactggg tttgcctgat gatggtgtag acggcctgca cattctgcct gaagatgcgc ctgtcggtac caacatccgc gaatacttgg atttggacga tatgctgttt acgttgaaaa ttacgcctaa ccgcgctgat tgcttgagtg ttaagggcat tgcgcgtgag gtttctgcgt tgactcaatg tgcgtttacg cctgttgaaa ttcagacggc ccctatcggc agtgagaaaa aacaggctgt ccgtattgat gcaccggctg actgcggccg ttttatcagc cgcgttattg aaaatgtcaa cgcgaaagcg gctactcctg attggatgaa acaacgtttg gaacgcagcg gtatccgcag catttccgca ttggtggaca ttggcaacta tgtgatgctg gaaatcggtc agcctatgca tgttttcgat gctgataagc tgtcgggcag tttgattgtc cgccgtgctc aaaatggcga gactttggcg tgtctgaatg agaagacggt tactttggct gacaatacac tggtggtcgc tgatgaaaaa ggtgcgttga gcttggccgg cttgatgggt ggcgcggcga gcgcggtttc agacggcacg caaaatatcg tgctggaagc ggcttggttt gagcccgaaa tcatcgtcgg caaatcgcgc caatacggtt tcggttcgga ttcgtcgttc cgcttcgagc gcggcgtgga ttaccgtttg caggcggatg ccattgaacg cgccaccgaa ttggttttgc agatttgcgg cggtgcggca ggcgagatgg tggaagcaca aggtaaattg cccgaagcca agcaggtcgg attgcgtttg ggtcgtctga aaaccgtgtt gggcgtggac attcctgccg aacaggtgga aaccattttg caacacttgg gtctgcagcc tgagaaaacg gcggaaggct tccgcattac cgcgccgagc ttccgtttcg acatcgaaat tgaggctgat ttgattgaag aaatcggacg cgtttacggc tatgaaaaca tccccgacga ttacacgtca ggccgtctga aaatgtcgga actgcccgaa acacgccgtc cgcgttttgc cgtttacaac gaaatggcgg cgcgcggcta ccgcgaagtg atcagctatg ccttcgttga cgagcagtgg gaactcgatt ttgccgccaa cgccgccccc atccgcctgc aaaacccgct ggcggcgcaa tatgccgtga tgcgttctac gttgattggc ggcttagtgg aaattttgca aaacaacctg aaccgcaagc aaaaccgcgt gcgcgtgttt gaaatcgcct gcgtgttcgg caaaggttca gacggccggt ttgtccaaaa cgaacgcatc ggcggtctgt ggtacggcgc ggcgatgccc gaacaatggg gtgagaaaac gcgcaatgcg gatttttacg acatcaaggc ggacgtggaa aatctgttga aaaacaaagc ggtcgagttc gttaaaaccg gacatcccgc cctgcatccc ggacgtgccg ccaatatcgt ttcagacggc aaagtcatcg gctttgtcgg cgaactgcat ccgaaatggc tgcaaaaata cgacctgccg caagcgccgc tggtatttga aatcgatatg gcggctgtgt tggaatgcgg gaaaacgcgc tatcgggccg tatcgaaatt ccagccggtg cgccgcgatt tggcgtttgt gatgccggaa gttatgaatc atgatgattt actgcttgtc ttgaaaggcg cagcaaacaa gttggtacag gaaatcagcg tgtttgacgt gtatcgcggc acgggtctgc ccgaagggat gaagagcgtg gcggttaaag tgattttgca ggatatggaa aacacgctga cggatgaggc ggtcgagccg cttattggaa aactgattgg cgtggcaacg gaggcagggg cgcggcttcg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for NegoA.18890.a.B5.GE42623
HARVESTED ON: 8/5/2018
SEQUENCED ON: 8/8/2018
EXPECTED MW: 86kDa
OBSERVED MW: 86kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHYS WLKTQANPDL SADKLEHLLT MAGLEVEEID TAAPAFSGVV VAEVKSVEKH PDADRLNVTQ VDAGTGELVQ IVCGAPNVKP GIKVPCSLPV AVLPGNFKIK PTKMRGVPSN GMLCSTNELG LPDDGVDGLH ILPEDAPVGT NIREYLDLDD MLFTLKITPN RADCLSVKGI AREVSALTQC AFTPVEIQTA PIGSEKKQAV RIDAPADCGR FISRVIENVN AKAATPDWMK QRLERSGIRS ISALVDIGNY VMLEIGQPMH VFDADKLSGS LIVRRAQNGE TLACLNEKTV TLADNTLVVA DEKGALSLAG LMGGAASAVS DGTQNIVLEA AWFEPEIIVG KSRQYGFGSD SSFRFERGVD YRLQADAIER ATELVLQICG GAAGEMVEAQ GKLPEAKQVG LRLGRLKTVL GVDIPAEQVE TILQHLGLQP EKTAEGFRIT APSFRFDIEI EADLIEEIGR VYGYENIPDD YTSGRLKMSE LPETRRPRFA VYNEMAARGY REVISYAFVD EQWELDFAAN AAPIRLQNPL AAQYAVMRST LIGGLVEILQ NNLNRKQNRV RVFEIACVFG KGSDGRFVQN ERIGGLWYGA AMPEQWGEKT RNADFYDIKA DVENLLKNKA VEFVKTGHPA LHPGRAANIV SDGKVIGFVG ELHPKWLQKY DLPQAPLVFE IDMAAVLECG KTRYRAVSKF QPVRRDLAFV MPEVMNHDDL LLVLKGAANK LVQEISVFDV YRGTGLPEGM KSVAVKVILQ DMENTLTDEA VEPLIGKLIG VATEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattactcat ggctgaaaac ccaagccaat cctgatcttt ctgccgataa actggaacat cttttgacca tggccggttt ggaagtggaa gaaatcgata ctgccgcccc tgctttcagt ggcgtggttg ttgccgaagt aaaatctgtt gaaaaacatc ctgatgcaga ccgtttgaac gttactcaag ttgatgccgg tacgggcgag ttggttcaga ttgtttgtgg tgcgccgaat gtcaagccgg gcattaaagt gccgtgttcg ttgccggttg ccgttttgcc gggcaatttt aaaattaagc cgactaaaat gcgcggcgta ccatcaaacg gtatgctgtg ttcgaccaat gaactgggtt tgcctgatga tggtgtagac ggcctgcaca ttctgcctga agatgcgcct gtcggtacca acatccgcga atacttggat ttggacgata tgctgtttac gttgaaaatt acgcctaacc gcgctgattg cttgagtgtt aagggcattg cgcgtgaggt ttctgcgttg actcaatgtg cgtttacgcc tgttgaaatt cagacggccc ctatcggcag tgagaaaaaa caggctgtcc gtattgatgc accggctgac tgcggccgtt ttatcagccg cgttattgaa aatgtcaacg cgaaagcggc tactcctgat tggatgaaac aacgtttgga acgcagcggt atccgcagca tttccgcatt ggtggacatt ggcaactatg tgatgctgga aatcggtcag cctatgcatg ttttcgatgc tgataagctg tcgggcagtt tgattgtccg ccgtgctcaa aatggcgaga ctttggcgtg tctgaatgag aagacggtta ctttggctga caatacactg gtggtcgctg atgaaaaagg tgcgttgagc ttggccggct tgatgggtgg cgcggcgagc gcggtttcag acggcacgca aaatatcgtg ctggaagcgg cttggtttga gcccgaaatc atcgtcggca aatcgcgcca atacggtttc ggttcggatt cgtcgttccg cttcgagcgc ggcgtggatt accgtttgca ggcggatgcc attgaacgcg ccaccgaatt ggttttgcag atttgcggcg gtgcggcagg cgagatggtg gaagcacaag gtaaattgcc cgaagccaag caggtcggat tgcgtttggg tcgtctgaaa accgtgttgg gcgtggacat tcctgccgaa caggtggaaa ccattttgca acacttgggt ctgcagcctg agaaaacggc ggaaggcttc cgcattaccg cgccgagctt ccgtttcgac atcgaaattg aggctgattt gattgaagaa atcggacgcg tttacggcta tgaaaacatc cccgacgatt acacgtcagg ccgtctgaaa atgtcggaac tgcccgaaac acgccgtccg cgttttgccg tttacaacga aatggcggcg cgcggctacc gcgaagtgat cagctatgcc ttcgttgacg agcagtggga actcgatttt gccgccaacg ccgcccccat ccgcctgcaa aacccgctgg cggcgcaata tgccgtgatg cgttctacgt tgattggcgg cttagtggaa attttgcaaa acaacctgaa ccgcaagcaa aaccgcgtgc gcgtgtttga aatcgcctgc gtgttcggca aaggttcaga cggccggttt gtccaaaacg aacgcatcgg cggtctgtgg tacggcgcgg cgatgcccga acaatggggt gagaaaacgc gcaatgcgga tttttacgac atcaaggcgg acgtggaaaa tctgttgaaa aacaaagcgg tcgagttcgt taaaaccgga catcccgccc tgcatcccgg acgtgccgcc aatatcgttt cagacggcaa agtcatcggc tttgtcggcg aactgcatcc gaaatggctg caaaaatacg acctgccgca agcgccgctg gtatttgaaa tcgatatggc ggctgtgttg gaatgcggga aaacgcgcta tcgggccgta tcgaaattcc agccggtgcg ccgcgatttg gcgtttgtga tgccggaagt tatgaatcat gatgatttac tgcttgtctt gaaaggcgca gcaaacaagt tggtacagga aatcagcgtg tttgacgtgt atcgcggcac gggtctgccc gaagggatga agagcgtggc ggttaaagtg attttgcagg atatggaaaa cacgctgacg gatgaggcgg tcgagccgct tattggaaaa ctgattggcg tggcaacgga ggcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for NegoA.18890.a.B1.PW37929
PURIFICATION DATe: 7/12/2016
CONCENTRATION: 32.5mg/ml
OBSERVED MW: 87kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN EKTVTLADNT LVVADEKGAL SLAGLMGGAA SAVSDGTQNI VLEAAWFEPE IIVGKSRQYG FGSDSSFRFE RGVDYRLQAD AIERATELVL QICGGAAGEM VEAQGKLPEA KQVGLRLGRL KTVLGVDIPA EQVETILQHL GLQPEKTAEG FRITAPSFRF DIEIEADLIE EIGRVYGYEN IPDDYTSGRL KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LECGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLRS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAT TCTCCTACTC ATGGCTGAAA ACCCAAGCCA ATCCTGATCT TTCTGCCGAT AAACTGGAAC ATCTTTTGAC CATGGCCGGT TTGGAAGTGG AAGAAATCGA TACTGCCGCC CCTGCTTTCA GTGGCGTGGT TGTTGCCGAA GTAAAATCTG TTGAAAAACA TCCTGATGCA GACCGTTTGA ACGTTACTCA AGTTGATGCC GGTACGGGCG AGTTGGTTCA GATTGTTTGT GGTGCGCCGA ATGTCAAGCC GGGCATTAAA GTGCCGTGTT CGTTGCCGGT TGCCGTTTTG CCGGGCAATT TTAAAATTAA GCCGACTAAA ATGCGCGGCG TACCATCAAA CGGTATGCTG TGTTCGACCA ATGAACTGGG TTTGCCTGAT GATGGTGTAG ACGGCCTGCA CATTCTGCCT GAAGATGCGC CTGTCGGTAC CAACATCCGC GAATACTTGG ATTTGGACGA TATGCTGTTT ACGTTGAAAA TTACGCCTAA CCGCGCTGAT TGCTTGAGTG TTAAGGGCAT TGCGCGTGAG GTTTCTGCGT TGACTCAATG TGCGTTTACG CCTGTTGAAA TTCAGACGGC CCCTATCGGC AGTGAGAAAA AACAGGCTGT CCGTATTGAT GCACCGGCTG ACTGCGGCCG TTTTATCAGC CGCGTTATTG AAAATGTCAA CGCGAAAGCG GCTACTCCTG ATTGGATGAA ACAACGTTTG GAACGCAGCG GTATCCGCAG CATTTCCGCA TTGGTGGACA TTGGCAACTA TGTGATGCTG GAAATCGGTC AGCCTATGCA TGTTTTCGAT GCTGATAAGC TGTCGGGCAG TTTGATTGTC CGCCGTGCTC AAAATGGCGA GACTTTGGCG TGTCTGAATG AGAAGACGGT TACTTTGGCT GACAATACAC TGGTGGTCGC TGATGAAAAA GGTGCGTTGA GCTTGGCCGG CTTGATGGGT GGCGCGGCGA GCGCGGTTTC AGACGGCACG CAAAATATCG TGCTGGAAGC GGCTTGGTTT GAGCCCGAAA TCATCGTCGG CAAATCGCGC CAATACGGTT TCGGTTCGGA TTCGTCGTTC CGCTTCGAGC GCGGCGTGGA TTACCGTTTG CAGGCGGATG CCATTGAACG CGCCACCGAA TTGGTTTTGC AGATTTGCGG CGGTGCGGCA GGCGAGATGG TGGAAGCACA AGGTAAATTG CCCGAAGCCA AGCAGGTCGG ATTGCGTTTG GGTCGTCTGA AAACCGTGTT GGGCGTGGAC ATTCCTGCCG AACAGGTGGA AACCATTTTG CAACACTTGG GTCTGCAGCC TGAGAAAACG GCGGAAGGCT TCCGCATTAC CGCGCCGAGC TTCCGTTTCG ACATCGAAAT TGAGGCTGAT TTGATTGAAG AAATCGGACG CGTTTACGGC TATGAAAACA TCCCCGACGA TTACACGTCA GGCCGTCTGA AAATGTCGGA ACTGCCCGAA ACACGCCGTC CGCGTTTTGC CGTTTACAAC GAAATGGCGG CGCGCGGCTA CCGCGAAGTG ATCAGCTATG CCTTCGTTGA CGAGCAGTGG GAACTCGATT TTGCCGCCAA CGCCGCCCCC ATCCGCCTGC AAAACCCGCT GGCGGCGCAA TATGCCGTGA TGCGTTCTAC GTTGATTGGC GGCTTAGTGG AAATTTTGCA AAACAACCTG AACCGCAAGC AAAACCGCGT GCGCGTGTTT GAAATCGCCT GCGTGTTCGG CAAAGGTTCA GACGGCCGGT TTGTCCAAAA CGAACGCATC GGCGGTCTGT GGTACGGCGC GGCGATGCCC GAACAATGGG GTGAGAAAAC GCGCAATGCG GATTTTTACG ACATCAAGGC GGACGTGGAA AATCTGTTGA AAAACAAAGC GGTCGAGTTC GTTAAAACCG GACATCCCGC CCTGCATCCC GGACGTGCCG CCAATATCGT TTCAGACGGC AAAGTCATCG GCTTTGTCGG CGAACTGCAT CCGAAATGGC TGCAAAAATA CGACCTGCCG CAAGCGCCGC TGGTATTTGA AATCGATATG GCGGCTGTGT TGGAATGCGG GAAAACGCGC TATCGGGCCG TATCGAAATT CCAGCCGGTG CGCCGCGATT TGGCGTTTGT GATGCCGGAA GTTATGAATC ATGATGATTT ACTGCTTGTC TTGAAAGGCG CAGCAAACAA GTTGGTACAG GAAATCAGCG TGTTTGACGT GTATCGCGGC ACGGGTCTGC CCGAAGGGAT GAAGAGCGTG GCGGTTAAAG TGATTTTGCA GGATATGGAA AACACGCTGA CGGATGAGGC GGTCGAGCCG CTTATTGGAA AACTGATTGG CGTGGCAACG GAGGCAGGGG CGCGGCTTCG CAGTTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for NegoA.18890.a.B11.PW38732
PURIFICATION DATe: 10/4/2019
CONCENTRATION: 10mg/ml
OBSERVED MW: 87kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide, 1 mM ATP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 10
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 78
Protocol Notes
combined with 44022
Validated AA Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN XKTXTLGXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XPDDYTSGRX KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LECGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLRS
Validated NT Sequence
atggctcacc accaccacca ccatatgcaa ttctcctact catggctgaa aacccaagcc aatcctgatc tttctgccga taaactggaa catcttttga ccatggccgg tttggaagtg gaagaaatcg atactgccgc ccctgctttc agtggcgtgg ttgttgccga agtaaaatct gttgaaaaac atcctgatgc agaccgtttg aacgttactc aagttgatgc cggtacgggc gagttggttc agattgtttg tggtgcgccg aatgtcaagc cgggcattaa agtgccgtgt tcgttgccgg ttgccgtttt gccgggcaat tttaaaatta agccgactaa aatgcgcggc gtaccatcaa acggtatgct gtgttcgacc aatgaactgg gtttgcctga tgatggtgta gacggcctgc acattctgcc tgaagatgcg cctgtcggta ccaacatccg cgaatacttg gatttggacg atatgctgtt tacgttgaaa attacgccta accgcgctga ttgcttgagt gttaagggca ttgcgcgtga ggtttctgcg ttgactcaat gtgcgtttac gcctgttgaa attcagacgg cccctatcgg cagtgagaaa aaacaggctg tccgtattga tgcaccggct gactgcggcc gttttatcag ccgcgttatt gaaaatgtca acgcgaaagc ggctactcct gattggatga aacaacgttt ggaacgcagc ggtatccgca gcatttccgc attggtggac attggcaact atgtgatgct ggaaatcggt cagcctatgc atgttttcga tgctgataag ctgtcgggca gtttgattgt ccgccgtgct caaaatggcg agactttggc gtgtctgaat ganaagacgg ntactttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccggacgatt acacgtcagg ccgtntgaaa atgtcggaac tgcccgaaac acgccgtccg cgttttgccg tttacaacga aatggcggcg cgcggctacc gcgaagtgat cagctatgcn ttcgttgacg agcagtggga actngatttt gccgccaacg ccgcccccat ccgcctgcaa aacccgctgg cggcgcaata tgccgtgatg cgttctacgt tgattggcgg nttagtggaa attttgcaaa acaacctgaa ccgcaagcaa aaccgcgtgc gcgtgtttga aatcgcctgc gtgttcggca aaggttcaga cggccggttt gtccaaaacg aacgcatcgg cggtctgtgg tacggcgcgg cgatgcccga acaatggggt gagaaaacgc gcaatgcgga tttttacgac atcaaggcgg acgtggaaaa tctgttgaaa aacaaagcgg tcgagttcgt taaaaccgga catcccgccc tgcatcccgg acgtgccgcc aatatcgttt cagacggcaa agtcatcggc tttgtcggcg aactgcatcc gaaatggctg caaaaatacg acctgccgca agcgccgctg gtatttgaaa tcgatatggc ggctgtgttg gaatgcggga aaacgcgcta tcgggccgta tcgaaattcc agccggtgcg ccgcgatttg gcgtttgtga tgccggaagt tatgaatcat gatgatttac tgcttgtctt gaaaggcgca gcaaacaagt tggtacagga aatcagcgtg tttgacgtgt atcgcggcac gggtctgccc gaagggatga agagcgtggc ggttaaagtg attttgcagg atatggaaaa cacgctgacg gatgaggcgg tcgagccgct tattggaaaa ctgattggcg tggcaacgga ggcaggggcg cggcttcgca gttaa
Expressed Protein Sequence
MAHHHHHHMQ FSYSWLKTQA NPDLSADKLE HLLTMAGLEV EEIDTAAPAF SGVVVAEVKS VEKHPDADRL NVTQVDAGTG ELVQIVCGAP NVKPGIKVPC SLPVAVLPGN FKIKPTKMRG VPSNGMLCST NELGLPDDGV DGLHILPEDA PVGTNIREYL DLDDMLFTLK ITPNRADCLS VKGIAREVSA LTQCAFTPVE IQTAPIGSEK KQAVRIDAPA DCGRFISRVI ENVNAKAATP DWMKQRLERS GIRSISALVD IGNYVMLEIG QPMHVFDADK LSGSLIVRRA QNGETLACLN EKTVTLADNT LVVADEKGAL SLAGLMGGAA SAVSDGTQNI VLEAAWFEPE IIVGKSRQYG FGSDSSFRFE RGVDYRLQAD AIERATELVL QICGGAAGEM VEAQGKLPEA KQVGLRLGRL KTVLGVDIPA EQVETILQHL GLQPEKTAEG FRITAPSFRF DIEIEADLIE EIGRVYGYEN IPDDYTSGRL KMSELPETRR PRFAVYNEMA ARGYREVISY AFVDEQWELD FAANAAPIRL QNPLAAQYAV MRSTLIGGLV EILQNNLNRK QNRVRVFEIA CVFGKGSDGR FVQNERIGGL WYGAAMPEQW GEKTRNADFY DIKADVENLL KNKAVEFVKT GHPALHPGRA ANIVSDGKVI GFVGELHPKW LQKYDLPQAP LVFEIDMAAV LESGKTRYRA VSKFQPVRRD LAFVMPEVMN HDDLLLVLKG AANKLVQEIS VFDVYRGTGL PEGMKSVAVK VILQDMENTL TDEAVEPLIG KLIGVATEAG ARLRS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAT TCT CCTACTCATG GCTGAAAACC CAAGCCAATC CTGATCTTTC TGCCGATAAA CTGGAACATC TTTTGACCAT GGCCGGTTTG GAAGTGGAAG AAATCGATAC TGCCGCCCCT GCTTTCAGTG GCGTGGTTGT TGCCGAAGTA AAATCTGTTG AAAAACATCC TGATGCAGAC CGTTTGAACG TTACTCAAGT TGATGCCGGT ACGGGCGAGT TGGTTCAGAT TGTTTGTGGT GCGCCGAATG TCAAGCCGGG CATTAAAGTG CCGTGTTCGT TGCCGGTTGC CGTTTTGCCG GGCAATTTTA AAATTAAGCC GACTAAAATG CGCGGCGTAC CATCAAACGG TATGCTGTGT TCGACCAATG AACTGGGTTT GCCTGATGAT GGTGTAGACG GCCTGCACAT TCTGCCTGAA GATGCGCCTG TCGGTACCAA CATCCGCGAA TACTTGGATT TGGACGATAT GCTGTTTACG TTGAAAATTA CGCCTAACCG CGCTGATTGC TTGAGTGTTA AGGGCATTGC GCGTGAGGTT TCTGCGTTGA CTCAATGTGC GTTTACGCCT GTTGAAATTC AGACGGCCCC TATCGGCAGT GAGAAAAAAC AGGCTGTCCG TATTGATGCA CCGGCTGACT GCGGCCGTTT TATCAGCCGC GTTATTGAAA ATGTCAACGC GAAAGCGGCT ACTCCTGATT GGATGAAACA ACGTTTGGAA CGCAGCGGTA TCCGCAGCAT TTCCGCATTG GTGGACATTG GCAACTATGT GATGCTGGAA ATCGGTCAGC CTATGCATGT TTTCGATGCT GATAAGCTGT CGGGCAGTTT GATTGTCCGC CGTGCTCAAA ATGGCGAGAC TTTGGCGTGT CTGAATGAGA AGACGGTTAC TTTGGCTGAC AATACACTGG TGGTCGCTGA TGAAAAAGGT GCGTTGAGCT TGGCCGGCTT GATGGGTGGC GCGGCGAGCG CGGTTTCAGA CGGCACGCAA AATATCGTGC TGGAAGCGGC TTGGTTTGAG CCCGAAATCA TCGTCGGCAA ATCGCGCCAA TACGGTTTCG GTTCGGATTC GTCGTTCCGC TTCGAGCGCG GCGTGGATTA CCGTTTGCAG GCGGATGCCA TTGAACGCGC CACCGAATTG GTTTTGCAGA TTTGCGGCGG TGCGGCAGGC GAGATGGTGG AAGCACAAGG TAAATTGCCC GAAGCCAAGC AGGTCGGATT GCGTTTGGGT CGTCTGAAAA CCGTGTTGGG CGTGGACATT CCTGCCGAAC AGGTGGAAAC CATTTTGCAA CACTTGGGTC TGCAGCCTGA GAAAACGGCG GAAGGCTTCC GCATTACCGC GCCGAGCTTC CGTTTCGACA TCGAAATTGA GGCTGATTTG ATTGAAGAAA TCGGACGCGT TTACGGCTAT GAAAACATCC CCGACGATTA CACGTCAGGC CGTCTGAAAA TGTCGGAACT GCCCGAAACA CGCCGTCCGC GTTTTGCCGT TTACAACGAA ATGGCGGCGC GCGGCTACCG CGAAGTGATC AGCTATGCCT TCGTTGACGA GCAGTGGGAA CTCGATTTTG CCGCCAACGC CGCCCCCATC CGCCTGCAAA ACCCGCTGGC GGCGCAATAT GCCGTGATGC GTTCTACGTT GATTGGCGGC TTAGTGGAAA TTTTGCAAAA CAACCTGAAC CGCAAGCAAA ACCGCGTGCG CGTGTTTGAA ATCGCCTGCG TGTTCGGCAA AGGTTCAGAC GGCCGGTTTG TCCAAAACGA ACGCATCGGC GGTCTGTGGT ACGGCGCGGC GATGCCCGAA CAATGGGGTG AGAAAACGCG CAATGCGGAT TTTTACGACA TCAAGGCGGA CGTGGAAAAT CTGTTGAAAA ACAAAGCGGT CGAGTTCGTT AAAACCGGAC ATCCCGCCCT GCATCCCGGA CGTGCCGCCA ATATCGTTTC AGACGGCAAA GTCATCGGCT TTGTCGGCGA ACTGCATCCG AAATGGCTGC AAAAATACGA CCTGCCGCAA GCGCCGCTGG TATTTGAAAT CGATATGGCG GCTGTGTTGG AAAGCGGGAA AACGCGCTAT CGGGCCGTAT CGAAATTCCA GCCGGTGCGC CGCGATTTGG CGTTTGTGAT GCCGGAAGTT ATGAATCATG ATGATTTACT GCTTGTCTTG AAAGGCGCAG CAAACAAGTT GGTACAGGAA ATCAGCGTGT TTGACGTGTA TCGCGGCACG GGTCTGCCCG AAGGGATGAA GAGCGTGGCG GTTAAAGTGA TTTTGCAGGA TATGGAAAAC ACGCTGACGG ATGAGGCGGT CGAGCCGCTT ATTGGAAAAC TGATTGGCGT GGCAACGGAG GCAGGGGCGC GGCTTCGCAG TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT