NegoA.18708.a

isoleucyl-tRNA synthetase

CENTER ID:	NegoA.18708.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.18708.a.B1.GE40326	full length		1	929

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.18708.a.B1.PW37937	full length		1	929

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_85052

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.110
UniProt:	B4RPY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDYSKTVNL LESPFPMRGN LAKREPAWLK SWYEQKRYQK LREIAKGRPK FILHDGPPYA NGDIHIGHAV NKILKDIIIR SKTQAGFDAP YVPGWDCHGL PIEVMVEKLH GKDMPKARFR ELCREYAAEQ IARQKKDFIR LGVLGDWDNP YLTMDFKTEA DTVRMLGEIY KSGYLYRGAK PVQFCLDCGS SLAEAEVEYK DKVSPAIDVA YPFKDTAALA AAFGLAGIEG KAFAVIWTTT PWTLPASQAV SAGADVVYQL IDTPKGKLVL AKDLAEGALK RYGFSDGIAI LAETTGDKLE NLHMNHPFLE RDIPMLNGEH VTTDAGTGLV HTAPAHGLED YAVCNKYGIE LYNPVNAEGK YISETPRVAG MSVWEANPVI LQWPEETGNL LASSKIEHSY AHCWRHKTPL IYRATGQWFV GMDKAGSDGK TLRDKAIKAV DDTEFFPPWG RARLESMIEG RPDWVVSRQR YWGTPMTFFV HKETGELHPN SAELLEKVAQ RIEEKGIEAW FSLDKSELLS AEDCEHYDKL PDTMDVWFDS GSTHYSVVKQ REELEWPADL YLEGSDQHRG WFQSSMLTGC ASSMGRAPYK QLLTHGFVVD QNGRKMSKSI GNVVAPQEVY NEFGADILRL WAASTDYSGE LAISKEILKR VTESYRRIRN TLSFLFANLS DFNPIGDAVQ QADMVEIDRY ALVLARRLQE RLAGGYYPRY AFHFAVKDIV SFCSEDLGAF YLDILKDRLY TTKADSRARR SAQTALYHIT RSLVLLIAPI LCFTGEEAWD IIGGGEEDSV LFHTWHEFPA INEKAEAELV KKWTAIREAR EAVTAAIEPL RADKTVGSSL QAEAEITAPE EMAGYLNALG EELRFALLVS KAEVKVGDEL AVAAKASDGE KCERCWHYTR DVGAVAGYET VCKRCAENVG GEGETRHYA

NT Sequence

ATGACCGATT ACAGTAAAAC CGTCAACCTG CTCGAAAGCC CGTTTCCGAT GCGCGGCAAT CTTGCCAAGC GCGAGCCTGC GTGGCTGAAA AGCTGGTACG AGCAAAAACG TTACCAAAAA CTGCGCGAAA TCGCCAAAGG CCGTCCGAAA TTCATCCTGC ACGACGGCCC GCCGTATGCC AACGGCGACA TCCATATCGG TCATGCCGTC AACAAAATCC TTAAAGACAT TATTATCCGC AGCAAAACCC AAGCCGGTTT TGACGCGCCT TATGTACCGG GTTGGGACTG CCACGGCCTG CCCATCGAAG TGATGGTGGA AAAGCTGCAC GGCAAAGATA TGCCTAAAGC CCGTTTCCGC GAATTGTGCC GCGAATATGC CGCCGAACAG ATTGCCCGTC AGAAAAAAGA CTTTATCCGC TTGGGCGTGT TGGGCGATTG GGACAATCCT TACTTGACTA TGGATTTCAA AACCGAAGCC GATACCGTGC GTATGCTCGG CGAAATCTAC AAATCCGGCT ATCTCTACCG TGGTGCGAAA CCGGTTCAGT TTTGCTTGGA TTGCGGATCT TCGCTGGCGG AAGCGGAAGT GGAATACAAA GACAAAGTAT CGCCTGCGAT TGACGTTGCC TATCCGTTTA AAGACACTGC CGCGCTTGCC GCCGCATTCG GCTTGGCAGG TATCGAAGGC AAAGCGTTTG CCGTCATTTG GACGACCACG CCTTGGACTC TGCCTGCGAG CCAGGCCGTG TCTGCCGGCG CGGACGTGGT GTATCAATTA ATCGATACGC CCAAAGGCAA ATTGGTGCTG GCGAAAGATT TGGCGGAAGG CGCTTTGAAA CGCTACGGCT TTTCAGACGG CATCGCCATC CTTGCCGAAA CCACCGGCGA CAAGCTGGAA AACCTGCACA TGAATCATCC GTTCCTCGAA CGCGATATTC CCATGCTCAA CGGCGAACAC GTTACCACCG ATGCCGGTAC CGGCTTGGTG CATACTGCGC CTGCGCACGG TTTGGAAGAC TACGCCGTCT GCAATAAATA CGGCATCGAG CTTTACAACC CTGTCAACGC CGAAGGCAAA TACATAAGCG AAACGCCTCG TGTCGCAGGC ATGAGCGTTT GGGAGGCGAA TCCCGTCATC CTGCAATGGC CGGAAGAAAC CGGCAACCTC TTGGCAAGCA GCAAAATCGA ACACAGCTAC GCCCACTGCT GGCGCCACAA AACCCCGCTG ATTTACCGAG CGACAGGTCA GTGGTTTGTC GGCATGGACA AAGCCGGCAG CGACGGTAAA ACCCTGCGCG ACAAAGCCAT CAAAGCCGTG GACGACACCG AATTCTTCCC GCCATGGGGT CGTGCGCGTT TGGAATCCAT GATTGAAGGC CGTCCTGACT GGGTGGTTTC ACGCCAACGC TATTGGGGCA CGCCGATGAC TTTCTTTGTT CACAAAGAAA CGGGCGAGCT GCATCCGAAC TCTGCCGAAC TTTTGGAAAA AGTCGCGCAA CGCATCGAAG AAAAAGGCAT CGAGGCTTGG TTCTCCCTCG ATAAAAGCGA ATTATTAAGC GCGGAAGATT GCGAACATTA CGACAAACTC CCCGATACCA TGGACGTATG GTTCGACTCA GGCTCGACGC ATTATTCCGT TGTAAAACAA CGCGAAGAAT TGGAATGGCC GGCTGACTTG TACCTCGAAG GCAGCGACCA ACACCGCGGC TGGTTCCAAT CCTCTATGCT GACCGGTTGC GCCTCATCCA TGGGACGCGC ACCGTATAAA CAGCTGCTGA CCCACGGTTT CGTGGTTGAC CAAAACGGCC GCAAAATGTC GAAATCCATC GGCAACGTCG TCGCGCCGCA AGAAGTCTAT AACGAATTCG GCGCGGACAT CCTGCGCCTG TGGGCGGCAT CCACCGATTA CAGCGGCGAA TTGGCGATTT CCAAAGAAAT CCTCAAACGC GTTACCGAAA GCTACCGCCG CATCCGCAAT ACCTTGAGCT TTCTGTTTGC CAATTTGAGC GACTTTAATC CGATTGGAGA TGCCGTGCAA CAGGCGGATA TGGTGGAAAT CGACCGCTAC GCCTTGGTAT TGGCGCGGCG GCTGCAAGAG CGTTTGGCAG GCGGTTACTA TCCGCGCTAT GCCTTCCACT TCGCCGTGAA AGACATTGTT TCTTTCTGCT CGGAAGACTT GGGCGCGTTC TACCTCGACA TCCTGAAAGA CCGCCTCTAC ACCACCAAAG CCGACAGCCG CGCCCGCCGC AGCGCGCAAA CCGCCCTGTA CCACATCACG CGCAGCCTGG TTCTCTTGAT TGCACCGATT TTGTGCTTCA CCGGCGAAGA AGCGTGGGAC ATCATCGGCG GCGGCGAAGA AGACAGCGTC CTCTTCCATA CTTGGCACGA GTTCCCGGCC ATCAACGAAA AAGCCGAAGC CGAACTGGTG AAAAAATGGA CGGCAATCCG CGAAGCGCGC GAAGCAGTAA CCGCCGCCAT CGAGCCTTTG CGCGCCGACA AAACCGTCGG TTCGTCCTTG CAGGCGGAAG CCGAAATTAC CGCGCCGGAA GAAATGGCCG GCTATCTGAA TGCTTTGGGC GAAGAATTGC GCTTTGCCCT GCTGGTGTCT AAAGCAGAAG TGAAAGTCGG TGATGAACTT GCCGTTGCCG CCAAAGCCAG CGACGGCGAA AAATGCGAAC GCTGCTGGCA TTACACCCGC GATGTGGGCG CGGTTGCAGG TTATGAAACC GTCTGCAAAC GCTGTGCGGA GAATGTCGGC GGAGAAGGCG AAACGCGCCA TTACGCCTGA

Details for NegoA.18708.a.B1.GE40326

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/29/2016
EXPECTED MW:	105kDa
OBSERVED MW:	102kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT DYSKTVNLLE SPFPMRGNLA KREPAWLKSW YEQKRYQKLR EIAKGRPKFI LHDGPPYANG DIHIGHAVNK ILKDIIIRSK TQAGFDAPYV PGWDCHGLPI EVMVEKLHGK DMPKARFREL CREYAAEQIA RQKKDFIRLG VLGDWDNPYL TMDFKTEADT VRMLGEIYKS GYLYRGAKPV QFCLDCGSSL AEAEVEYKDK VSPAIDVAYP FKDTAALAAA FGLAGIEGKA FAVIWTTTPW TLPASQAVSA GADVVYQLID TPKGKLVLAK DLAEGALKRY GFSDGIAILA ETTGDKLENL HMNHPFLERD IPMLNGEHVT TDAGTGLVHT APAHGLEDYA VCNKYGIELY NPVNAEGKYI SETPRVAGMS VWEANPVILQ WPEETGNLLA SSKIEHSYAH CWRHKTPLIY RATGQWFVGM DKAGSDGKTL RDKAIKAVDD TEFFPPWGRA RLESMIEGRP DWVVSRQRYW GTPMTFFVHK ETGELHPNSA ELLEKVAQRI EEKGIEAWFS LDKSELLSAE DCEHYDKLPD TMDVWFDSGS THYSVVKQRE ELEWPADLYL EGSDQHRGWF QSSMLTGCAS SMGRAPYKQL LTHGFVVDQN GRKMSKSIGN VVAPQEVYNE FGADILRLWA ASTDYSGELA ISKEILKRVT ESYRRIRNTL SFLFANLSDF NPIGDAVQQA DMVEIDRYAL VLARRLQERL AGGYYPRYAF HFAVKDIVSF CSEDLGAFYL DILKDRLYTT KADSRARRSA QTALYHITRS LVLLIAPILC FTGEEAWDII GGGEEDSVLF HTWHEFPAIN EKAEAELVKK WTAIREAREA VTAAIEPLRA DKTVGSSLQA EAEITAPEEM AGYLNALGEE LRFALLVSKA EVKVGDELAV AAKASDGEKC ERCWHYTRDV GAVAGYETVC KRCAENVGGE GETRHYA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg attacagtaa aaccgtcaac ctgctcgaaa gcccgtttcc gatgcgcggc aatcttgcca agcgcgagcc tgcgtggctg aaaagctggt acgagcaaaa acgttaccaa aaactgcgcg aaatcgccaa aggccgtccg aaattcatcc tgcacgacgg cccgccgtat gccaacggcg acatccatat cggtcatgcc gtcaacaaaa tccttaaaga cattattatc cgcagcaaaa cccaagccgg ttttgacgcg ccttatgtac cgggttggga ctgccacggc ctgcccatcg aagtgatggt ggaaaagctg cacggcaaag atatgcctaa agcccgtttc cgcgaattgt gccgcgaata tgccgccgaa cagattgccc gtcagaaaaa agactttatc cgcttgggcg tgttgggcga ttgggacaat ccttacttga ctatggattt caaaaccgaa gccgataccg tgcgtatgct cggcgaaatc tacaaatccg gctatctcta ccgtggtgcg aaaccggttc agttttgctt ggattgcgga tcttcgctgg cggaagcgga agtggaatac aaagacaaag tatcgcctgc gattgacgtt gcctatccgt ttaaagacac tgccgcgctt gccgccgcat tcggcttggc aggtatcgaa ggcaaagcgt ttgccgtcat ttggacgacc acgccttgga ctctgcctgc gagccaggcc gtgtctgccg gcgcggacgt ggtgtatcaa ttaatcgata cgcccaaagg caaattggtg ctggcgaaag atttggcgga aggcgctttg aaacgctacg gcttttcaga cggcatcgcc atccttgccg aaaccaccgg cgacaagctg gaaaacctgc acatgaatca tccgttcctc gaacgcgata ttcccatgct caacggcgaa cacgttacca ccgatgccgg taccggcttg gtgcatactg cgcctgcgca cggtttggaa gactacgccg tctgcaataa atacggcatc gagctttaca accctgtcaa cgccgaaggc aaatacataa gcgaaacgcc tcgtgtcgca ggcatgagcg tttgggaggc gaatcccgtc atcctgcaat ggccggaaga aaccggcaac ctcttggcaa gcagcaaaat cgaacacagc tacgcccact gctggcgcca caaaaccccg ctgatttacc gagcgacagg tcagtggttt gtcggcatgg acaaagccgg cagcgacggt aaaaccctgc gcgacaaagc catcaaagcc gtggacgaca ccgaattctt cccgccatgg ggtcgtgcgc gtttggaatc catgattgaa ggccgtcctg actgggtggt ttcacgccaa cgctattggg gcacgccgat gactttcttt gttcacaaag aaacgggcga gctgcatccg aactctgccg aacttttgga aaaagtcgcg caacgcatcg aagaaaaagg catcgaggct tggttctccc tcgataaaag cgaattatta agcgcggaag attgcgaaca ttacgacaaa ctccccgata ccatggacgt atggttcgac tcaggctcga cgcattattc cgttgtaaaa caacgcgaag aattggaatg gccggctgac ttgtacctcg aaggcagcga ccaacaccgc ggctggttcc aatcctctat gctgaccggt tgcgcctcat ccatgggacg cgcaccgtat aaacagctgc tgacccacgg tttcgtggtt gaccaaaacg gccgcaaaat gtcgaaatcc atcggcaacg tcgtcgcgcc gcaagaagtc tataacgaat tcggcgcgga catcctgcgc ctgtgggcgg catccaccga ttacagcggc gaattggcga tttccaaaga aatcctcaaa cgcgttaccg aaagctaccg ccgcatccgc aataccttga gctttctgtt tgccaatttg agcgacttta atccgattgg agatgccgtg caacaggcgg atatggtgga aatcgaccgc tacgccttgg tattggcgcg gcggctgcaa gagcgtttgg caggcggtta ctatccgcgc tatgccttcc acttcgccgt gaaagacatt gtttctttct gctcggaaga cttgggcgcg ttctacctcg acatcctgaa agaccgcctc tacaccacca aagccgacag ccgcgcccgc cgcagcgcgc aaaccgccct gtaccacatc acgcgcagcc tggttctctt gattgcaccg attttgtgct tcaccggcga agaagcgtgg gacatcatcg gcggcggcga agaagacagc gtcctcttcc atacttggca cgagttcccg gccatcaacg aaaaagccga agccgaactg gtgaaaaaat ggacggcaat ccgcgaagcg cgcgaagcag taaccgccgc catcgagcct ttgcgcgccg acaaaaccgt cggttcgtcc ttgcaggcgg aagccgaaat taccgcgccg gaagaaatgg ccggctatct gaatgctttg ggcgaagaat tgcgctttgc cctgctggtg tctaaagcag aagtgaaagt cggtgatgaa cttgccgttg ccgccaaagc cagcgacggc gaaaaatgcg aacgctgctg gcattacacc cgcgatgtgg gcgcggttgc aggttatgaa accgtctgca aacgctgtgc ggagaatgtc ggcggagaag gcgaaacgcg ccattacgcc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for NegoA.18708.a.B1.PW37937

PURIFICATION DATe:	8/8/2016
CONCENTRATION:	14mg/ml
OBSERVED MW:	105kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG ATTACAGTAA AACCGTCAAC CTGCTCGAAA GCCCGTTTCC GATGCGCGGC AATCTTGCCA AGCGCGAGCC TGCGTGGCTG AAAAGCTGGT ACGAGCAAAA ACGTTACCAA AAACTGCGCG AAATCGCCAA AGGCCGTCCG AAATTCATCC TGCACGACGG CCCGCCGTAT GCCAACGGCG ACATCCATAT CGGTCATGCC GTCAACAAAA TCCTTAAAGA CATTATTATC CGCAGCAAAA CCCAAGCCGG TTTTGACGCG CCTTATGTAC CGGGTTGGGA CTGCCACGGC CTGCCCATCG AAGTGATGGT GGAAAAGCTG CACGGCAAAG ATATGCCTAA AGCCCGTTTC CGCGAATTGT GCCGCGAATA TGCCGCCGAA CAGATTGCCC GTCAGAAAAA AGACTTTATC CGCTTGGGCG TGTTGGGCGA TTGGGACAAT CCTTACTTGA CTATGGATTT CAAAACCGAA GCCGATACCG TGCGTATGCT CGGCGAAATC TACAAATCCG GCTATCTCTA CCGTGGTGCG AAACCGGTTC AGTTTTGCTT GGATTGCGGA TCTTCGCTGG CGGAAGCGGA AGTGGAATAC AAAGACAAAG TATCGCCTGC GATTGACGTT GCCTATCCGT TTAAAGACAC TGCCGCGCTT GCCGCCGCAT TCGGCTTGGC AGGTATCGAA GGCAAAGCGT TTGCCGTCAT TTGGACGACC ACGCCTTGGA CTCTGCCTGC GAGCCAGGCC GTGTCTGCCG GCGCGGACGT GGTGTATCAA TTAATCGATA CGCCCAAAGG CAAATTGGTG CTGGCGAAAG ATTTGGCGGA AGGCGCTTTG AAACGCTACG GCTTTTCAGA CGGCATCGCC ATCCTTGCCG AAACCACCGG CGACAAGCTG GAAAACCTGC ACATGAATCA TCCGTTCCTC GAACGCGATA TTCCCATGCT CAACGGCGAA CACGTTACCA CCGATGCCGG TACCGGCTTG GTGCATACTG CGCCTGCGCA CGGTTTGGAA GACTACGCCG TCTGCAATAA ATACGGCATC GAGCTTTACA ACCCTGTCAA CGCCGAAGGC AAATACATAA GCGAAACGCC TCGTGTCGCA GGCATGAGCG TTTGGGAGGC GAATCCCGTC ATCCTGCAAT GGCCGGAAGA AACCGGCAAC CTCTTGGCAA GCAGCAAAAT CGAACACAGC TACGCCCACT GCTGGCGCCA CAAAACCCCG CTGATTTACC GAGCGACAGG TCAGTGGTTT GTCGGCATGG ACAAAGCCGG CAGCGACGGT AAAACCCTGC GCGACAAAGC CATCAAAGCC GTGGACGACA CCGAATTCTT CCCGCCATGG GGTCGTGCGC GTTTGGAATC CATGATTGAA GGCCGTCCTG ACTGGGTGGT TTCACGCCAA CGCTATTGGG GCACGCCGAT GACTTTCTTT GTTCACAAAG AAACGGGCGA GCTGCATCCG AACTCTGCCG AACTTTTGGA AAAAGTCGCG CAACGCATCG AAGAAAAAGG CATCGAGGCT TGGTTCTCCC TCGATAAAAG CGAATTATTA AGCGCGGAAG ATTGCGAACA TTACGACAAA CTCCCCGATA CCATGGACGT ATGGTTCGAC TCAGGCTCGA CGCATTATTC CGTTGTAAAA CAACGCGAAG AATTGGAATG GCCGGCTGAC TTGTACCTCG AAGGCAGCGA CCAACACCGC GGCTGGTTCC AATCCTCTAT GCTGACCGGT TGCGCCTCAT CCATGGGACG CGCACCGTAT AAACAGCTGC TGACCCACGG TTTCGTGGTT GACCAAAACG GCCGCAAAAT GTCGAAATCC ATCGGCAACG TCGTCGCGCC GCAAGAAGTC TATAACGAAT TCGGCGCGGA CATCCTGCGC CTGTGGGCGG CATCCACCGA TTACAGCGGC GAATTGGCGA TTTCCAAAGA AATCCTCAAA CGCGTTACCG AAAGCTACCG CCGCATCCGC AATACCTTGA GCTTTCTGTT TGCCAATTTG AGCGACTTTA ATCCGATTGG AGATGCCGTG CAACAGGCGG ATATGGTGGA AATCGACCGC TACGCCTTGG TATTGGCGCG GCGGCTGCAA GAGCGTTTGG CAGGCGGTTA CTATCCGCGC TATGCCTTCC ACTTCGCCGT GAAAGACATT GTTTCTTTCT GCTCGGAAGA CTTGGGCGCG TTCTACCTCG ACATCCTGAA AGACCGCCTC TACACCACCA AAGCCGACAG CCGCGCCCGC CGCAGCGCGC AAACCGCCCT GTACCACATC ACGCGCAGCC TGGTTCTCTT GATTGCACCG ATTTTGTGCT TCACCGGCGA AGAAGCGTGG GACATCATCG GCGGCGGCGA AGAAGACAGC GTCCTCTTCC ATACTTGGCA CGAGTTCCCG GCCATCAACG AAAAAGCCGA AGCCGAACTG GTGAAAAAAT GGACGGCAAT CCGCGAAGCG CGCGAAGCAG TAACCGCCGC CATCGAGCCT TTGCGCGCCG ACAAAACCGT CGGTTCGTCC TTGCAGGCGG AAGCCGAAAT TACCGCGCCG GAAGAAATGG CCGGCTATCT GAATGCTTTG GGCGAAGAAT TGCGCTTTGC CCTGCTGGTG TCTAAAGCAG AAGTGAAAGT CGGTGATGAA CTTGCCGTTG CCGCCAAAGC CAGCGACGGC GAAAAATGCG AACGCTGCTG GCATTACACC CGCGATGTGG GCGCGGTTGC AGGTTATGAA ACCGTCTGCA AACGCTGTGC GGAGAATGTC GGCGGAGAAG GCGAAACGCG CCATTACGCC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT