NegoA.18222.a

glutaminyl-tRNA synthetase

CENTER ID: NegoA.18222.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.18222.a.B1.GE40146 full length 1 573
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.18222.a.B1.PS38035 full length 1 573

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.325
UniProt: B4RJ33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSEAICLKK TMLNKDQFAD NHFIRTIIED DLKSGKHEAV QTRFPPEPNG YLHIGHAKSI CLNFGLAYIY DGLCNLRFDD TNPEKENDEY VNAIKEDVEW LGFHWAGEPR FASDYFDRLY DYAVGLIKDG KAYVDDLTPE EMREYRGTLT EAGKNSPYRD RSIEENLDLF TRMKNGEFPD GSKTLRLKID MAAGNINMRD PVIYRIRRAH HHNTGDKWCI YPMYDYTHCI SDAIEGITHS LCTLEFEAHR PLYDWVLDNI PAPHATRPRQ YEFSRLELLY TITSKRKLNQ LVVEKHVSGW DDPRMPTISG MRRRGYTPEG VRLFAKRAGI SKSENIVDMS VLEGAIREEL ENSAPRLMAV LNPLKVTLTN FQAGKTQSRR AAFHPNHEEM GDREVPVSQT IYIEADDFAE NPPKGFKRLI PGGEVRLRHG YVIKCGEVVK DEAGNVVELK CSIDHDTLGK NPEGRKVKGV IHWVSAEHAA EIKVRLYDRL FTVERPGAVR GEDGEYLPFT DFLNPESVKE ITAYAEPAAK DLPAESRWQF ERIGYFVTDR QDHGKDTPVF NRTVTLKDSW QPK
NT Sequence
ATGCCGTCTG AAGCCATCTG TTTAAAGAAA ACCATGCTCA ATAAAGACCA ATTCGCGGAC AACCATTTCA TCCGCACCAT CATCGAAGAC GACCTCAAAA GCGGCAAACA CGAAGCCGTC CAAACCCGTT TTCCGCCCGA ACCCAACGGC TATTTACATA TCGGACACGC CAAATCCATC TGCCTGAACT TCGGTTTGGC GTATATTTAC GACGGTTTGT GCAACCTGCG TTTCGACGAC ACCAACCCGG AAAAAGAAAA CGACGAATAC GTCAACGCCA TCAAAGAAGA CGTCGAGTGG CTCGGTTTCC ATTGGGCGGG CGAACCGCGT TTCGCTTCCG ACTATTTCGA CCGGCTTTAT GACTACGCCG TCGGTTTAAT CAAAGACGGC AAAGCGTATG TCGATGATTT GACGCCCGAA GAAATGCGAG AATACCGCGG TACGCTGACC GAAGCGGGTA AAAACAGCCC TTACCGCGAC CGCAGTATCG AAGAAAACCT CGACCTGTTC ACGCGTATGA AAAACGGCGA GTTCCCCGAC GGCAGCAAAA CCCTGCGCCT GAAAATCGAC ATGGCGGCAG GCAACATCAA TATGCGCGAT CCCGTCATCT ACCGCATCCG CCGCGCCCAT CACCACAACA CCGGTGACAA ATGGTGCATC TACCCGATGT ACGATTACAC GCATTGCATT TCCGATGCCA TCGAAGGCAT CACGCATTCC TTGTGCACGC TCGAATTTGA AGCGCACCGT CCGCTTTACG ACTGGGTGTT GGACAACATC CCCGCGCCGC ACGCCACCCG TCCGCGCCAA TACGAGTTTT CCCGTTTGGA GCTTTTGTAC ACCATTACCT CCAAACGGAA ATTGAATCAG TTGGTTGTGG AAAAACACGT TTCCGGCTGG GATGATCCGC GTATGCCGAC CATTTCCGGT ATGCGCCGCC GCGGCTACAC GCCCGAAGGG GTGCGCCTGT TTGCCAAACG CGCCGGTATT TCCAAATCTG AAAACATCGT CGACATGAGC GTGTTGGAGG GTGCGATTCG CGAAGAGCTG GAAAACTCCG CCCCGCGCCT GATGGCGGTT TTGAACCCGC TCAAAGTGAC CCTGACCAAC TTTCAAGCCG GCAAAACCCA AAGCCGCCGT GCCGCGTTCC ATCCGAACCA CGAAGAAATG GGCGATCGCG AAGTACCTGT TTCACAAACC ATCTACATCG AAGCCGACGA CTTTGCCGAA AATCCGCCCA AAGGCTTCAA GCGTCTGATT CCCGGCGGCG AAGTACGCTT GCGCCACGGC TATGTCATCA AGTGCGGTGA AGTCGTCAAA GACGAAGCAG GCAATGTGGT TGAACTCAAA TGCAGCATCG ACCACGACAC CTTGGGCAAA AATCCAGAAG GCCGCAAAGT TAAAGGCGTG ATTCACTGGG TTTCCGCCGA ACACGCCGCC GAAATCAAAG TCCGTCTGTA CGACCGCCTC TTTACCGTCG AGCGTCCCGG TGCCGTGCGC GGCGAAGACG GCGAATACCT GCCGTTTACC GATTTCCTCA ATCCGGAATC CGTTAAGGAA ATCACTGCTT ACGCCGAACC TGCCGCGAAA GATTTGCCGG CGGAAAGCCG TTGGCAGTTC GAGCGCATCG GCTATTTTGT GACCGACCGC CAAGACCACG GCAAAGACAC GCCGGTGTTT AACCGCACGG TGACGTTGAA AGATTCTTGG CAGCCTAAGT AA
Details for NegoA.18222.a.B1.GE40146
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMP SEAICLKKTM LNKDQFADNH FIRTIIEDDL KSGKHEAVQT RFPPEPNGYL HIGHAKSICL NFGLAYIYDG LCNLRFDDTN PEKENDEYVN AIKEDVEWLG FHWAGEPRFA SDYFDRLYDY AVGLIKDGKA YVDDLTPEEM REYRGTLTEA GKNSPYRDRS IEENLDLFTR MKNGEFPDGS KTLRLKIDMA AGNINMRDPV IYRIRRAHHH NTGDKWCIYP MYDYTHCISD AIEGITHSLC TLEFEAHRPL YDWVLDNIPA PHATRPRQYE FSRLELLYTI TSKRKLNQLV VEKHVSGWDD PRMPTISGMR RRGYTPEGVR LFAKRAGISK SENIVDMSVL EGAIREELEN SAPRLMAVLN PLKVTLTNFQ AGKTQSRRAA FHPNHEEMGD REVPVSQTIY IEADDFAENP PKGFKRLIPG GEVRLRHGYV IKCGEVVKDE AGNVVELKCS IDHDTLGKNP EGRKVKGVIH WVSAEHAAEI KVRLYDRLFT VERPGAVRGE DGEYLPFTDF LNPESVKEIT AYAEPAAKDL PAESRWQFER IGYFVTDRQD HGKDTPVFNR TVTLKDSWQP K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgt ctgaagccat ctgtttaaag aaaaccatgc tcaataaaga ccaattcgcg gacaaccatt tcatccgcac catcatcgaa gacgacctca aaagcggcaa acacgaagcc gtccaaaccc gttttccgcc cgaacccaac ggctatttac atatcggaca cgccaaatcc atctgcctga acttcggttt ggcgtatatt tacgacggtt tgtgcaacct gcgtttcgac gacaccaacc cggaaaaaga aaacgacgaa tacgtcaacg ccatcaaaga agacgtcgag tggctcggtt tccattgggc gggcgaaccg cgtttcgctt ccgactattt cgaccggctt tatgactacg ccgtcggttt aatcaaagac ggcaaagcgt atgtcgatga tttgacgccc gaagaaatgc gagaataccg cggtacgctg accgaagcgg gtaaaaacag cccttaccgc gaccgcagta tcgaagaaaa cctcgacctg ttcacgcgta tgaaaaacgg cgagttcccc gacggcagca aaaccctgcg cctgaaaatc gacatggcgg caggcaacat caatatgcgc gatcccgtca tctaccgcat ccgccgcgcc catcaccaca acaccggtga caaatggtgc atctacccga tgtacgatta cacgcattgc atttccgatg ccatcgaagg catcacgcat tccttgtgca cgctcgaatt tgaagcgcac cgtccgcttt acgactgggt gttggacaac atccccgcgc cgcacgccac ccgtccgcgc caatacgagt tttcccgttt ggagcttttg tacaccatta cctccaaacg gaaattgaat cagttggttg tggaaaaaca cgtttccggc tgggatgatc cgcgtatgcc gaccatttcc ggtatgcgcc gccgcggcta cacgcccgaa ggggtgcgcc tgtttgccaa acgcgccggt atttccaaat ctgaaaacat cgtcgacatg agcgtgttgg agggtgcgat tcgcgaagag ctggaaaact ccgccccgcg cctgatggcg gttttgaacc cgctcaaagt gaccctgacc aactttcaag ccggcaaaac ccaaagccgc cgtgccgcgt tccatccgaa ccacgaagaa atgggcgatc gcgaagtacc tgtttcacaa accatctaca tcgaagccga cgactttgcc gaaaatccgc ccaaaggctt caagcgtctg attcccggcg gcgaagtacg cttgcgccac ggctatgtca tcaagtgcgg tgaagtcgtc aaagacgaag caggcaatgt ggttgaactc aaatgcagca tcgaccacga caccttgggc aaaaatccag aaggccgcaa agttaaaggc gtgattcact gggtttccgc cgaacacgcc gccgaaatca aagtccgtct gtacgaccgc ctctttaccg tcgagcgtcc cggtgccgtg cgcggcgaag acggcgaata cctgccgttt accgatttcc tcaatccgga atccgttaag gaaatcactg cttacgccga acctgccgcg aaagatttgc cggcggaaag ccgttggcag ttcgagcgca tcggctattt tgtgaccgac cgccaagacc acggcaaaga cacgccggtg tttaaccgca cggtgacgtt gaaagattct tggcagccta agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for NegoA.18222.a.B1.PS38035
PURIFICATION DATe: 8/23/2016
CONCENTRATION: 48.7mg/ml
OBSERVED MW: 67kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMP SEAICLKKTM LNKDQFADNH FIRTIIEDDL KSGKHEAVQT RFPPEPNGYL HIGHAKSICL NFGLAYIYDG LCNLRFDDTN PEKENDEYVN AIKEDVEWLG FHWAGEPRFA SDYFDRLYDY AVGLIKDGKA YVDDLTPEEM REYRGTLTEA GKNSPYRDRS IEENLDLFTR MKNGEFPDGS KTLRLKIDMA AGNINMRDPV IYRIRRAHHH NTGDKWCIYP MYDYTHCISD AIEGITHSLC TLEFEAHRPL YDWVLDNIPA PHATRPRQYE FSRLELLYTI TSKRKLNQLV VEKHVSGWDD PRMPTISGMR RRGYTPEGVR LFAKRAGISK SENIVDMSVL EGAIREELEN SAPRLMAVLN PLKVTLTNFQ AGKTQSRRAA FHPNHEEMGD REVPVSQTIY IEADDFAENP PKGFKRLIPG GEVRLRHGYV IKCGEVVKDE AGNVVELKCS IDHDTLGKNP EGRKVKGVIH WVSAEHAAEI KVRLYDRLFT VERPGAVRGE DGEYLPFTDF LNPESVKEIT AYAEPAAKDL PAESRWQFER IGYFVTDRQD HGKDTPVFNR TVTLKDSWQP K
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGT CTGAAGCCAT CTGTTTAAAG AAAACCATGC TCAATAAAGA CCAATTCGCG GACAACCATT TCATCCGCAC CATCATCGAA GACGACCTCA AAAGCGGCAA ACACGAAGCC GTCCAAACCC GTTTTCCGCC CGAACCCAAC GGCTATTTAC ATATCGGACA CGCCAAATCC ATCTGCCTGA ACTTCGGTTT GGCGTATATT TACGACGGTT TGTGCAACCT GCGTTTCGAC GACACCAACC CGGAAAAAGA AAACGACGAA TACGTCAACG CCATCAAAGA AGACGTCGAG TGGCTCGGTT TCCATTGGGC GGGCGAACCG CGTTTCGCTT CCGACTATTT CGACCGGCTT TATGACTACG CCGTCGGTTT AATCAAAGAC GGCAAAGCGT ATGTCGATGA TTTGACGCCC GAAGAAATGC GAGAATACCG CGGTACGCTG ACCGAAGCGG GTAAAAACAG CCCTTACCGC GACCGCAGTA TCGAAGAAAA CCTCGACCTG TTCACGCGTA TGAAAAACGG CGAGTTCCCC GACGGCAGCA AAACCCTGCG CCTGAAAATC GACATGGCGG CAGGCAACAT CAATATGCGC GATCCCGTCA TCTACCGCAT CCGCCGCGCC CATCACCACA ACACCGGTGA CAAATGGTGC ATCTACCCGA TGTACGATTA CACGCATTGC ATTTCCGATG CCATCGAAGG CATCACGCAT TCCTTGTGCA CGCTCGAATT TGAAGCGCAC CGTCCGCTTT ACGACTGGGT GTTGGACAAC ATCCCCGCGC CGCACGCCAC CCGTCCGCGC CAATACGAGT TTTCCCGTTT GGAGCTTTTG TACACCATTA CCTCCAAACG GAAATTGAAT CAGTTGGTTG TGGAAAAACA CGTTTCCGGC TGGGATGATC CGCGTATGCC GACCATTTCC GGTATGCGCC GCCGCGGCTA CACGCCCGAA GGGGTGCGCC TGTTTGCCAA ACGCGCCGGT ATTTCCAAAT CTGAAAACAT CGTCGACATG AGCGTGTTGG AGGGTGCGAT TCGCGAAGAG CTGGAAAACT CCGCCCCGCG CCTGATGGCG GTTTTGAACC CGCTCAAAGT GACCCTGACC AACTTTCAAG CCGGCAAAAC CCAAAGCCGC CGTGCCGCGT TCCATCCGAA CCACGAAGAA ATGGGCGATC GCGAAGTACC TGTTTCACAA ACCATCTACA TCGAAGCCGA CGACTTTGCC GAAAATCCGC CCAAAGGCTT CAAGCGTCTG ATTCCCGGCG GCGAAGTACG CTTGCGCCAC GGCTATGTCA TCAAGTGCGG TGAAGTCGTC AAAGACGAAG CAGGCAATGT GGTTGAACTC AAATGCAGCA TCGACCACGA CACCTTGGGC AAAAATCCAG AAGGCCGCAA AGTTAAAGGC GTGATTCACT GGGTTTCCGC CGAACACGCC GCCGAAATCA AAGTCCGTCT GTACGACCGC CTCTTTACCG TCGAGCGTCC CGGTGCCGTG CGCGGCGAAG ACGGCGAATA CCTGCCGTTT ACCGATTTCC TCAATCCGGA ATCCGTTAAG GAAATCACTG CTTACGCCGA ACCTGCCGCG AAAGATTTGC CGGCGGAAAG CCGTTGGCAG TTCGAGCGCA TCGGCTATTT TGTGACCGAC CGCCAAGACC ACGGCAAAGA CACGCCGGTG TTTAACCGCA CGGTGACGTT GAAAGATTCT TGGCAGCCTA AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT