SSGCID: Target Page

NegoA.18180.a

phosphogluconate dehydratase

CENTER ID:	NegoA.18180.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.18180.a.B1.GE40145	full length		1	611

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.18180.a.B1.PS38032	full length		1	611

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.1260
UniProt:	B4RM16

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNHTPIHPKL AEITGRIIER SRPTREKYLA KIRSAKQMGR LERNQLGCSN LAHGYAAMPK SIKIEMLQET VPNLGIITAY NDMVSAHQPF KDFPDQIKDE AQKNGATAQV AGGTPAMCDG ITQGYAGMEL SLFSRDVIAM STAVGLSHQM FDGSLFMGVC DKIVPGLMIG ALSFGHIPGI FVPAGPMSSG IGNKEKARTR QLFAEGKVGR DELLKSEMGS YHSPGTCTFY GTANSNQMMM EMMGVHLPAA AFVHPYTDLR EALTRYAAGH LARGIKNGTI KPLGEMLTEK SFINALIGLM ATGGSTNHTM HLVAMARAAG VILNWDDFDE ISSIIPLLIR VYPNGKADVN HFTAAGGLPF VIRELLDAGL LHDDVDTVVG HGMRHYTKEP FLIDGKLEWR EAPETSGNDD ILRKADNPFS PDGGLRLMKG NIGRGVIKVS AVREGCRIIE APAIVFNDQR EVLAAFERGE LERDFICVVR YQGPRANGMP ELHKLTPPLG ILQDRGFKVA LLTDGRMSGA SGKVPASIHM TPEALMGGNI AKIRTGDLIR FDSVTGELNV LINEAEWNVR EVERIDLGAN QQGCGRELFA GFRSMTSSAE TGAMSFGGEF A

NT Sequence

ATGAACCACA CTCCTATCCA CCCCAAACTC GCCGAAATCA CCGGGCGCAT TATCGAACGC AGCCGTCCGA CGCGTGAAAA ATATCTGGCG AAAATCCGCA GTGCCAAACA AATGGGACGC TTAGAGCGCA ACCAGCTCGG CTGCAGCAAT TTGGCGCACG GCTATGCCGC CATGCCCAAA AGCATCAAAA TTGAAATGCT TCAGGAAACC GTCCCCAACT TAGGCATCAT CACTGCCTAC AACGACATGG TTTCCGCACA CCAGCCGTTT AAAGACTTCC CTGACCAAAT CAAAGACGAA GCACAAAAAA ACGGCGCGAC CGCACAAGTC GCCGGCGGCA CGCCTGCCAT GTGCGACGGC ATCACGCAAG GCTACGCCGG CATGGAATTG TCGCTGTTTT CCCGCGACGT GATTGCGATG AGTACCGCCG TCGGGCTGTC GCACCAAATG TTTGACGGCA GCCTGTTTAT GGGCGTGTGC GACAAAATCG TTCCGGGTCT GATGATAGGT GCGCTTTCGT TCGGTCATAT TCCCGGTATC TTCGTTCCGG CAGGCCCGAT GTCCAGCGGC ATCGGCAATA AAGAAAAAGC CCGTACCCGC CAACTTTTCG CCGAAGGCAA AGTCGGACGC GACGAACTTT TGAAAAGCGA AATGGGTTCT TACCACAGCC CGGGCACCTG CACTTTCTAC GGCACGGCAA ACTCCAACCA AATGATGATG GAAATGATGG GCGTACACCT GCCTGCCGCC GCCTTTGTCC ACCCCTATAC CGACCTGCGC GAAGCCCTGA CCCGCTACGC CGCCGGACAC CTCGCGCGCG GCATCAAAAA CGGCACGATT AAACCTTTGG GCGAAATGTT GACCGAAAAA TCCTTCATCA ATGCCCTGAT CGGTTTGATG GCGACCGGCG GCTCGACCAA CCACACCATG CACCTCGTCG CTATGGCGCG CGCCGCAGGC GTAATTTTGA ACTGGGACGA CTTCGACGAA ATTTCTTCCA TCATCCCGCT GCTCATCCGC GTGTATCCCA ACGGCAAAGC CGACGTGAAC CACTTTACCG CAGCAGGCGG CCTGCCTTTC GTTATCCGCG AATTGCTGGA CGCAGGCTTA TTGCACGACG ATGTCGATAC CGTCGTCGGA CACGGTATGC GCCACTACAC TAAAGAGCCT TTCCTCATCG ACGGCAAACT CGAATGGCGC GAAGCCCCTG AGACCAGCGG CAACGACGAC ATCCTGCGTA AAGCCGACAA TCCGTTCTCC CCGGACGGCG GTCTGCGCCT GATGAAAGGC AACATCGGAC GCGGCGTGAT TAAAGTGTCA GCCGTACGCG AAGGCTGCCG CATCATCGAA GCACCCGCCA TCGTGTTCAA CGACCAGCGC GAAGTGTTGG CGGCATTTGA ACGCGGCGAG TTGGAACGCG ATTTTATCTG CGTCGTCCGC TACCAAGGCC CGCGTGCCAA CGGTATGCCC GAATTGCACA AACTGACCCC GCCTTTGGGC ATCCTGCAAG ACCGCGGCTT CAAAGTGGCG CTGCTGACCG ACGGCCGTAT GTCCGGCGCG TCAGGCAAAG TGCCCGCGTC CATCCACATG ACGCCCGAAG CCCTGATGGG CGGCAACATC GCCAAAATCC GTACCGGCGA CCTGATTCGC TTCGACTCCG TTACCGGCGA ACTCAACGTC CTGATTAACG AGGCCGAATG GAACGTCCGA GAAGTCGAGC GCATCGATTT GGGCGCGAAC CAACAAGGCT GCGGCCGCGA ACTCTTCGCC GGCTTCCGCA GTATGACCAG CAGTGCGGAA ACCGGTGCGA TGAGCTTCGG CGGAGAATTT GCCTGA

Details for NegoA.18180.a.B1.GE40145

HARVESTED ON:	4/6/2016
SEQUENCED ON:	4/13/2016
EXPECTED MW:	67kDa
OBSERVED MW:	67kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMN HTPIHPKLAE ITGRIIERSR PTREKYLAKI RSAKQMGRLE RNQLGCSNLA HGYAAMPKSI KIEMLQETVP NLGIITAYND MVSAHQPFKD FPDQIKDEAQ KNGATAQVAG GTPAMCDGIT QGYAGMELSL FSRDVIAMST AVGLSHQMFD GSLFMGVCDK IVPGLMIGAL SFGHIPGIFV PAGPMSSGIG NKEKARTRQL FAEGKVGRDE LLKSEMGSYH SPGTCTFYGT ANSNQMMMEM MGVHLPAAAF VHPYTDLREA LTRYAAGHLA RGIKNGTIKP LGEMLTEKSF INALIGLMAT GGSTNHTMHL VAMARAAGVI LNWDDFDEIS SIIPLLIRVY PNGKADVNHF TAAGGLPFVI RELLDAGLLH DDVDTVVGHG MRHYTKEPFL IDGKLEWREA PETSGNDDIL RKADNPFSPD GGLRLMKGNI GRGVIKVSAV REGCRIIEAP AIVFNDQREV LAAFERGELE RDFICVVRYQ GPRANGMPEL HKLTPPLGIL QDRGFKVALL TDGRMSGASG KVPASIHMTP EALMGGNIAK IRTGDLIRFD SVTGELNVLI NEAEWNVREV ERIDLGANQQ GCGRELFAGF RSMTSSAETG AMSFGGEFA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc acactcctat ccaccccaaa ctcgccgaaa tcaccgggcg cattatcgaa cgcagccgtc cgacgcgtga aaaatatctg gcgaaaatcc gcagtgccaa acaaatggga cgcttagagc gcaaccagct cggctgcagc aatttggcgc acggctatgc cgccatgccc aaaagcatca aaattgaaat gcttcaggaa accgtcccca acttaggcat catcactgcc tacaacgaca tggtttccgc acaccagccg tttaaagact tccctgacca aatcaaagac gaagcacaaa aaaacggcgc gaccgcacaa gtcgccggcg gcacgcctgc catgtgcgac ggcatcacgc aaggctacgc cggcatggaa ttgtcgctgt tttcccgcga cgtgattgcg atgagtaccg ccgtcgggct gtcgcaccaa atgtttgacg gcagcctgtt tatgggcgtg tgcgacaaaa tcgttccggg tctgatgata ggtgcgcttt cgttcggtca tattcccggt atcttcgttc cggcaggccc gatgtccagc ggcatcggca ataaagaaaa agcccgtacc cgccaacttt tcgccgaagg caaagtcgga cgcgacgaac ttttgaaaag cgaaatgggt tcttaccaca gcccgggcac ctgcactttc tacggcacgg caaactccaa ccaaatgatg atggaaatga tgggcgtaca cctgcctgcc gccgcctttg tccaccccta taccgacctg cgcgaagccc tgacccgcta cgccgccgga cacctcgcgc gcggcatcaa aaacggcacg attaaacctt tgggcgaaat gttgaccgaa aaatccttca tcaatgccct gatcggtttg atggcgaccg gcggctcgac caaccacacc atgcacctcg tcgctatggc gcgcgccgca ggcgtaattt tgaactggga cgacttcgac gaaatttctt ccatcatccc gctgctcatc cgcgtgtatc ccaacggcaa agccgacgtg aaccacttta ccgcagcagg cggcctgcct ttcgttatcc gcgaattgct ggacgcaggc ttattgcacg acgatgtcga taccgtcgtc ggacacggta tgcgccacta cactaaagag cctttcctca tcgacggcaa actcgaatgg cgcgaagccc ctgagaccag cggcaacgac gacatcctgc gtaaagccga caatccgttc tccccggacg gcggtctgcg cctgatgaaa ggcaacatcg gacgcggcgt gattaaagtg tcagccgtac gcgaaggctg ccgcatcatc gaagcacccg ccatcgtgtt caacgaccag cgcgaagtgt tggcggcatt tgaacgcggc gagttggaac gcgattttat ctgcgtcgtc cgctaccaag gcccgcgtgc caacggtatg cccgaattgc acaaactgac cccgcctttg ggcatcctgc aagaccgcgg cttcaaagtg gcgctgctga ccgacggccg tatgtccggc gcgtcaggca aagtgcccgc gtccatccac atgacgcccg aagccctgat gggcggcaac atcgccaaaa tccgtaccgg cgacctgatt cgcttcgact ccgttaccgg cgaactcaac gtcctgatta acgaggccga atggaacgtc cgagaagtcg agcgcatcga tttgggcgcg aaccaacaag gctgcggccg cgaactcttc gccggcttcc gcagtatgac cagcagtgcg gaaaccggtg cgatgagctt cggcggagaa tttgcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for NegoA.18180.a.B1.PS38032

PURIFICATION DATe:	8/23/2016
CONCENTRATION:	10.81mg/ml
OBSERVED MW:	67kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	26
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC ACACTCCTAT CCACCCCAAA CTCGCCGAAA TCACCGGGCG CATTATCGAA CGCAGCCGTC CGACGCGTGA AAAATATCTG GCGAAAATCC GCAGTGCCAA ACAAATGGGA CGCTTAGAGC GCAACCAGCT CGGCTGCAGC AATTTGGCGC ACGGCTATGC CGCCATGCCC AAAAGCATCA AAATTGAAAT GCTTCAGGAA ACCGTCCCCA ACTTAGGCAT CATCACTGCC TACAACGACA TGGTTTCCGC ACACCAGCCG TTTAAAGACT TCCCTGACCA AATCAAAGAC GAAGCACAAA AAAACGGCGC GACCGCACAA GTCGCCGGCG GCACGCCTGC CATGTGCGAC GGCATCACGC AAGGCTACGC CGGCATGGAA TTGTCGCTGT TTTCCCGCGA CGTGATTGCG ATGAGTACCG CCGTCGGGCT GTCGCACCAA ATGTTTGACG GCAGCCTGTT TATGGGCGTG TGCGACAAAA TCGTTCCGGG TCTGATGATA GGTGCGCTTT CGTTCGGTCA TATTCCCGGT ATCTTCGTTC CGGCAGGCCC GATGTCCAGC GGCATCGGCA ATAAAGAAAA AGCCCGTACC CGCCAACTTT TCGCCGAAGG CAAAGTCGGA CGCGACGAAC TTTTGAAAAG CGAAATGGGT TCTTACCACA GCCCGGGCAC CTGCACTTTC TACGGCACGG CAAACTCCAA CCAAATGATG ATGGAAATGA TGGGCGTACA CCTGCCTGCC GCCGCCTTTG TCCACCCCTA TACCGACCTG CGCGAAGCCC TGACCCGCTA CGCCGCCGGA CACCTCGCGC GCGGCATCAA AAACGGCACG ATTAAACCTT TGGGCGAAAT GTTGACCGAA AAATCCTTCA TCAATGCCCT GATCGGTTTG ATGGCGACCG GCGGCTCGAC CAACCACACC ATGCACCTCG TCGCTATGGC GCGCGCCGCA GGCGTAATTT TGAACTGGGA CGACTTCGAC GAAATTTCTT CCATCATCCC GCTGCTCATC CGCGTGTATC CCAACGGCAA AGCCGACGTG AACCACTTTA CCGCAGCAGG CGGCCTGCCT TTCGTTATCC GCGAATTGCT GGACGCAGGC TTATTGCACG ACGATGTCGA TACCGTCGTC GGACACGGTA TGCGCCACTA CACTAAAGAG CCTTTCCTCA TCGACGGCAA ACTCGAATGG CGCGAAGCCC CTGAGACCAG CGGCAACGAC GACATCCTGC GTAAAGCCGA CAATCCGTTC TCCCCGGACG GCGGTCTGCG CCTGATGAAA GGCAACATCG GACGCGGCGT GATTAAAGTG TCAGCCGTAC GCGAAGGCTG CCGCATCATC GAAGCACCCG CCATCGTGTT CAACGACCAG CGCGAAGTGT TGGCGGCATT TGAACGCGGC GAGTTGGAAC GCGATTTTAT CTGCGTCGTC CGCTACCAAG GCCCGCGTGC CAACGGTATG CCCGAATTGC ACAAACTGAC CCCGCCTTTG GGCATCCTGC AAGACCGCGG CTTCAAAGTG GCGCTGCTGA CCGACGGCCG TATGTCCGGC GCGTCAGGCA AAGTGCCCGC GTCCATCCAC ATGACGCCCG AAGCCCTGAT GGGCGGCAAC ATCGCCAAAA TCCGTACCGG CGACCTGATT CGCTTCGACT CCGTTACCGG CGAACTCAAC GTCCTGATTA ACGAGGCCGA ATGGAACGTC CGAGAAGTCG AGCGCATCGA TTTGGGCGCG AACCAACAAG GCTGCGGCCG CGAACTCTTC GCCGGCTTCC GCAGTATGAC CAGCAGTGCG GAAACCGGTG CGATGAGCTT CGGCGGAGAA TTTGCCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T