NegoA.18178.a

ATP-dependent protease La

CENTER ID: NegoA.18178.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
NegoA.18178.a.A3.GE42461 Truncation 2 240 820
NegoA.18178.a.A4.GE42462 Truncation 3 252 820
NegoA.18178.a.B1.GE40247 full length 1 820
NegoA.18178.a.B3.GE43100 Truncation 2 240 820
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
NegoA.18178.a.A3.PW38560 Truncation 2 240 820
Molecular models
METHOD RESULTS
comparative modelling Robetta_85051
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|521006.8.peg.1184
UniProt: B4RLU1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQKEKHFEE YAALATLPLR DVVVYPHMVL PLFVGRPKSI AALENAITRE EPVFLLAQTD AAVEEPVATD LYQTGTVAQV LQVLKLPDGT VKVLVEGLYR GRVLTIEDTG GLFVSHIEAV VEEDTGGNTD LEAVRRTLLA QFEQYAKLNK KIPAEIIGSI NGIAENSRLT DTVAAHLQLK LAQRQQILEI PEIGKRMEFL LAKLESELDI MQAEKRIRGR VKRQMEKSQR EYYLNEQIKA IHKELGEEDE NGELDALEAG IKKAGMTKEA EEKCLSELKK LKMMPPMSAE STVVRNYIDT LLGLPWKKKS RVSKDIAKAG LVLDADHYGL EKVKERILEY LAVQKRMDKL KGPILCLVGP PGVGKTSLGE SIAKATGRKY VRMALGGVRD ESEIRGHRRT YIGSMPGKIL QNMAKAGVRN PLFLLDEIDK LGSDFRGDPA SALLEVLDHE QNNKFADHYA EVDYDLSDVM FIATSNSLNI PTPLLDRMEI IRLSGYTEDE KINIAMQYLV PKQMKRNGVK EGELVVEESA VRDIIRYYTR EAGVRSLDRE IAKICRKVVM QITLNEDKKR LSETKKTSKA KPRAVKVNEK NLHDYLGVRR FDYGVAESEN RIGQVTGLAW TEVGGELLTV EAAALPGKGM IQCTGQLGDV MKESVSAAWS VVRSRAESVG LAPDFYEKKD IHVHVPEGAT PKDGPSAGIA MTLAMVSAFT KIPVRADVAM TGEITLRGEV LPIGGLKEKL LAALRGGIKH VLIPKDNVKD LEEIPENVKT GLTIHPVKWI DEVLALGLES RPESWAEPSA AEAAAESASK PKPRSRAIKH
NT Sequence
ATGACACAAA AAGAAAAGCA TTTTGAGGAA TATGCTGCTT TGGCGACCCT TCCTTTACGG GATGTCGTCG TTTACCCGCA TATGGTTCTG CCGCTGTTCG TCGGCAGGCC GAAATCCATC GCCGCACTGG AAAACGCCAT TACCCGCGAG GAACCGGTTT TCCTGTTGGC GCAAACCGAT GCGGCGGTAG AAGAACCGGT TGCCACCGAC CTGTATCAGA CCGGTACCGT CGCACAAGTC CTGCAAGTGT TGAAACTGCC CGACGGCACG GTAAAAGTGC TGGTCGAAGG GCTGTATCGC GGACGTGTTT TGACCATCGA AGACACGGGC GGGCTGTTCG TTTCTCATAT AGAGGCGGTC GTGGAAGAAG ACACGGGCGG CAATACCGAC CTCGAAGCCG TGCGCCGCAC CCTGTTGGCG CAGTTTGAAC AATACGCCAA ACTCAATAAA AAAATCCCTG CCGAAATTAT CGGCAGCATC AACGGCATTG CCGAAAACAG CCGGCTAACC GATACGGTCG CAGCGCATTT GCAGTTGAAA CTGGCGCAAC GCCAACAGAT TTTGGAGATT CCCGAAATCG GCAAACGGAT GGAATTCCTG CTGGCAAAGC TGGAATCCGA ACTCGACATT ATGCAGGCCG AAAAACGCAT ACGCGGACGC GTCAAACGCC AAATGGAAAA ATCCCAACGC GAATATTATC TGAACGAACA GATTAAAGCG ATACACAAAG AATTGGGCGA AGAAGACGAA AACGGCGAAC TGGATGCCTT GGAGGCAGGC ATCAAAAAGG CGGGTATGAC CAAAGAAGCG GAAGAAAAAT GCCTGTCCGA ACTGAAAAAA CTCAAAATGA TGCCGCCGAT GTCTGCCGAA TCCACCGTCG TACGCAACTA CATCGACACC CTGCTCGGAC TGCCGTGGAA GAAAAAATCC CGCGTCAGCA AAGACATCGC CAAAGCCGGA CTGGTGCTGG ATGCCGACCA CTACGGCCTG GAAAAAGTCA AAGAACGGAT TTTGGAATAC CTCGCCGTCC AAAAACGTAT GGACAAGCTC AAAGGTCCGA TTCTGTGTTT GGTCGGCCCT CCGGGCGTGG GCAAAACCTC TTTGGGCGAA TCCATCGCCA AAGCAACGGG GCGGAAATAT GTCCGTATGG CCTTGGGCGG CGTGCGCGAC GAAAGCGAAA TCCGCGGGCA CCGCCGTACC TATATCGGCT CTATGCCCGG CAAGATTTTG CAGAATATGG CAAAAGCAGG CGTAAGAAAC CCGTTGTTCC TGCTCGACGA AATCGACAAA TTGGGCAGCG ACTTTCGGGG CGATCCCGCC AGCGCGTTGC TTGAAGTGCT CGATCACGAA CAAAACAACA AGTTCGCCGA TCATTATGCC GAAGTGGATT ACGATTTGAG CGACGTGATG TTTATTGCGA CTTCAAACAG CCTGAATATC CCGACCCCGC TGCTTGACCG TATGGAAATC ATCCGTCTGT CCGGCTATAC CGAAGACGAG AAAATCAATA TCGCGATGCA GTATCTCGTA CCGAAGCAAA TGAAACGCAA CGGCGTGAAA GAAGGCGAAT TGGTGGTCGA AGAAAGCGCG GTACGCGATA TTATCCGTTA TTACACCCGA GAGGCGGGCG TGCGTTCGCT CGACCGCGAA ATTGCCAAAA TCTGCCGTAA GGTGGTGATG CAGATTACCT TGAACGAAGA TAAGAAGAGG TTGTCTGAAA CCAAGAAAAC CAGCAAAGCC AAGCCTAGGG CGGTTAAAGT CAATGAGAAA AACCTACATG ACTACTTGGG CGTGCGCCGC TTCGATTACG GCGTTGCCGA AAGTGAAAAC CGCATCGGAC AGGTTACCGG TTTGGCTTGG ACGGAAGTCG GCGGCGAATT GCTGACTGTC GAAGCCGCAG CATTACCGGG TAAAGGCATG ATCCAGTGTA CCGGCCAGTT GGGCGATGTG ATGAAGGAAT CTGTATCCGC TGCATGGTCT GTTGTCCGTT CCCGTGCGGA ATCAGTAGGT TTGGCTCCTG ATTTTTACGA GAAAAAAGAC ATTCATGTCC ATGTTCCCGA AGGTGCAACG CCGAAAGACG GTCCGAGCGC AGGCATTGCT ATGACTTTGG CGATGGTGTC CGCCTTTACC AAAATTCCGG TGCGTGCCGA TGTTGCCATG ACGGGCGAAA TTACCCTGCG CGGCGAAGTC CTGCCGATCG GCGGTCTGAA GGAAAAACTG TTGGCTGCGC TTCGCGGCGG CATCAAACAC GTCCTGATTC CGAAAGACAA TGTCAAAGAC TTGGAAGAAA TCCCTGAAAA CGTGAAAACC GGATTAACCA TCCATCCGGT CAAATGGATA GACGAGGTAT TGGCTTTGGG TTTGGAAAGC CGGCCTGAGT CTTGGGCAGA ACCTTCTGCC GCGGAAGCGG CGGCGGAATC CGCTTCAAAA CCGAAACCCC GCAGCAGGGC AATCAAACAT TGA
Details for NegoA.18178.a.A3.GE42461
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SAIHKELGEE DENGELDALE AGIKKAGMTK EAEEKCLSEL KKLKMMPPMS AESTVVRNYI DTLLGLPWKK KSRVSKDIAK AGLVLDADHY GLEKVKERIL EYLAVQKRMD KLKGPILCLV GPPGVGKTSL GESIAKATGR KYVRMALGGV RDESEIRGHR RTYIGSMPGK ILQNMAKAGV RNPLFLLDEI DKLGSDFRGD PASALLEVLD HEQNNKFADH YAEVDYDLSD VMFIATSNSL NIPTPLLDRM EIIRLSGYTE DEKINIAMQY LVPKQMKRNG VKEGELVVEE SAVRDIIRYY TREAGVRSLD REIAKICRKV VMQITLNEDK KRLSETKKTS KAKPRAVKVN EKNLHDYLGV RRFDYGVAES ENRIGQVTGL AWTEVGGELL TVEAAALPGK GMIQCTGQLG DVMKESVSAA WSVVRSRAES VGLAPDFYEK KDIHVHVPEG ATPKDGPSAG IAMTLAMVSA FTKIPVRADV AMTGEITLRG EVLPIGGLKE KLLAALRGGI KHVLIPKDNV KDLEEIPENV KTGLTIHPVK WIDEVLALGL ESRPESWAEP SAAEAAAESA SKPKPRSRAI KH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggc gatacacaaa gaattgggcg aagaagacga aaacggcgaa ctggatgcct tggaggcagg catcaaaaag gcgggtatga ccaaagaagc ggaagaaaaa tgcctgtccg aactgaaaaa actcaaaatg atgccgccga tgtctgccga atccaccgtc gtacgcaact acatcgacac cctgctcgga ctgccgtgga agaaaaaatc ccgcgtcagc aaagacatcg ccaaagccgg actggtgctg gatgccgacc actacggcct ggaaaaagtc aaagaacgga ttttggaata cctcgccgtc caaaaacgta tggacaagct caaaggtccg attctgtgtt tggtcggccc tccgggcgtg ggcaaaacct ctttgggcga atccatcgcc aaagcaacgg ggcggaaata tgtccgtatg gccttgggcg gcgtgcgcga cgaaagcgaa atccgcgggc accgccgtac ctatatcggc tctatgcccg gcaagatttt gcagaatatg gcaaaagcag gcgtaagaaa cccgttgttc ctgctcgacg aaatcgacaa attgggcagc gactttcggg gcgatcccgc cagcgcgttg cttgaagtgc tcgatcacga acaaaacaac aagttcgccg atcattatgc cgaagtggat tacgatttga gcgacgtgat gtttattgcg acttcaaaca gcctgaatat cccgaccccg ctgcttgacc gtatggaaat catccgtctg tccggctata ccgaagacga gaaaatcaat atcgcgatgc agtatctcgt accgaagcaa atgaaacgca acggcgtgaa agaaggcgaa ttggtggtcg aagaaagcgc ggtacgcgat attatccgtt attacacccg agaggcgggc gtgcgttcgc tcgaccgcga aattgccaaa atctgccgta aggtggtgat gcagattacc ttgaacgaag ataagaagag gttgtctgaa accaagaaaa ccagcaaagc caagcctagg gcggttaaag tcaatgagaa aaacctacat gactacttgg gcgtgcgccg cttcgattac ggcgttgccg aaagtgaaaa ccgcatcgga caggttaccg gtttggcttg gacggaagtc ggcggcgaat tgctgactgt cgaagccgca gcattaccgg gtaaaggcat gatccagtgt accggccagt tgggcgatgt gatgaaggaa tctgtatccg ctgcatggtc tgttgtccgt tcccgtgcgg aatcagtagg tttggctcct gatttttacg agaaaaaaga cattcatgtc catgttcccg aaggtgcaac gccgaaagac ggtccgagcg caggcattgc tatgactttg gcgatggtgt ccgcctttac caaaattccg gtgcgtgccg atgttgccat gacgggcgaa attaccctgc gcggcgaagt cctgccgatc ggcggtctga aggaaaaact gttggctgcg cttcgcggcg gcatcaaaca cgtcctgatt ccgaaagaca atgtcaaaga cttggaagaa atccctgaaa acgtgaaaac cggattaacc atccatccgg tcaaatggat agacgaggta ttggctttgg gtttggaaag ccggcctgag tcttgggcag aaccttctgc cgcggaagcg gcggcggaat ccgcttcaaa accgaaaccc cgcagcaggg caatcaaaca ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for NegoA.18178.a.A4.GE42462
HARVESTED ON: 5/25/2018
SEQUENCED ON: 5/31/2018
EXPECTED MW: 65kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SGELDALEAG IKKAGMTKEA EEKCLSELKK LKMMPPMSAE STVVRNYIDT LLGLPWKKKS RVSKDIAKAG LVLDADHYGL EKVKERILEY LAVQKRMDKL KGPILCLVGP PGVGKTSLGE SIAKATGRKY VRMALGGVRD ESEIRGHRRT YIGSMPGKIL QNMAKAGVRN PLFLLDEIDK LGSDFRGDPA SALLEVLDHE QNNKFADHYA EVDYDLSDVM FIATSNSLNI PTPLLDRMEI IRLSGYTEDE KINIAMQYLV PKQMKRNGVK EGELVVEESA VRDIIRYYTR EAGVRSLDRE IAKICRKVVM QITLNEDKKR LSETKKTSKA KPRAVKVNEK NLHDYLGVRR FDYGVAESEN RIGQVTGLAW TEVGGELLTV EAAALPGKGM IQCTGQLGDV MKESVSAAWS VVRSRAESVG LAPDFYEKKD IHVHVPEGAT PKDGPSAGIA MTLAMVSAFT KIPVRADVAM TGEITLRGEV LPIGGLKEKL LAALRGGIKH VLIPKDNVKD LEEIPENVKT GLTIHPVKWI DEVLALGLES RPESWAEPSA AEAAAESASK PKPRSRAIKH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggg cgaactggat gccttggagg caggcatcaa aaaggcgggt atgaccaaag aagcggaaga aaaatgcctg tccgaactga aaaaactcaa aatgatgccg ccgatgtctg ccgaatccac cgtcgtacgc aactacatcg acaccctgct cggactgccg tggaagaaaa aatcccgcgt cagcaaagac atcgccaaag ccggactggt gctggatgcc gaccactacg gcctggaaaa agtcaaagaa cggattttgg aatacctcgc cgtccaaaaa cgtatggaca agctcaaagg tccgattctg tgtttggtcg gccctccggg cgtgggcaaa acctctttgg gcgaatccat cgccaaagca acggggcgga aatatgtccg tatggccttg ggcggcgtgc gcgacgaaag cgaaatccgc gggcaccgcc gtacctatat cggctctatg cccggcaaga ttttgcagaa tatggcaaaa gcaggcgtaa gaaacccgtt gttcctgctc gacgaaatcg acaaattggg cagcgacttt cggggcgatc ccgccagcgc gttgcttgaa gtgctcgatc acgaacaaaa caacaagttc gccgatcatt atgccgaagt ggattacgat ttgagcgacg tgatgtttat tgcgacttca aacagcctga atatcccgac cccgctgctt gaccgtatgg aaatcatccg tctgtccggc tataccgaag acgagaaaat caatatcgcg atgcagtatc tcgtaccgaa gcaaatgaaa cgcaacggcg tgaaagaagg cgaattggtg gtcgaagaaa gcgcggtacg cgatattatc cgttattaca cccgagaggc gggcgtgcgt tcgctcgacc gcgaaattgc caaaatctgc cgtaaggtgg tgatgcagat taccttgaac gaagataaga agaggttgtc tgaaaccaag aaaaccagca aagccaagcc tagggcggtt aaagtcaatg agaaaaacct acatgactac ttgggcgtgc gccgcttcga ttacggcgtt gccgaaagtg aaaaccgcat cggacaggtt accggtttgg cttggacgga agtcggcggc gaattgctga ctgtcgaagc cgcagcatta ccgggtaaag gcatgatcca gtgtaccggc cagttgggcg atgtgatgaa ggaatctgta tccgctgcat ggtctgttgt ccgttcccgt gcggaatcag taggtttggc tcctgatttt tacgagaaaa aagacattca tgtccatgtt cccgaaggtg caacgccgaa agacggtccg agcgcaggca ttgctatgac tttggcgatg gtgtccgcct ttaccaaaat tccggtgcgt gccgatgttg ccatgacggg cgaaattacc ctgcgcggcg aagtcctgcc gatcggcggt ctgaaggaaa aactgttggc tgcgcttcgc ggcggcatca aacacgtcct gattccgaaa gacaatgtca aagacttgga agaaatccct gaaaacgtga aaaccggatt aaccatccat ccggtcaaat ggatagacga ggtattggct ttgggtttgg aaagccggcc tgagtcttgg gcagaacctt ctgccgcgga agcggcggcg gaatccgctt caaaaccgaa accccgcagc agggcaatca aacattaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for NegoA.18178.a.B1.GE40247
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 92kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMT QKEKHFEEYA ALATLPLRDV VVYPHMVLPL FVGRPKSIAA LENAITREEP VFLLAQTDAA VEEPVATDLY QTGTVAQVLQ VLKLPDGTVK VLVEGLYRGR VLTIEDTGGL FVSHIEAVVE EDTGGNTDLE AVRRTLLAQF EQYAKLNKKI PAEIIGSING IAENSRLTDT VAAHLQLKLA QRQQILEIPE IGKRMEFLLA KLESELDIMQ AEKRIRGRVK RQMEKSQREY YLNEQIKAIH KELGEEDENG ELDALEAGIK KAGMTKEAEE KCLSELKKLK MMPPMSAEST VVRNYIDTLL GLPWKKKSRV SKDIAKAGLV LDADHYGLEK VKERILEYLA VQKRMDKLKG PILCLVGPPG VGKTSLGESI AKATGRKYVR MALGGVRDES EIRGHRRTYI GSMPGKILQN MAKAGVRNPL FLLDEIDKLG SDFRGDPASA LLEVLDHEQN NKFADHYAEV DYDLSDVMFI ATSNSLNIPT PLLDRMEIIR LSGYTEDEKI NIAMQYLVPK QMKRNGVKEG ELVVEESAVR DIIRYYTREA GVRSLDREIA KICRKVVMQI TLNEDKKRLS ETKKTSKAKP RAVKVNEKNL HDYLGVRRFD YGVAESENRI GQVTGLAWTE VGGELLTVEA AALPGKGMIQ CTGQLGDVMK ESVSAAWSVV RSRAESVGLA PDFYEKKDIH VHVPEGATPK DGPSAGIAMT LAMVSAFTKI PVRADVAMTG EITLRGEVLP IGGLKEKLLA ALRGGIKHVL IPKDNVKDLE EIPENVKTGL TIHPVKWIDE VLALGLESRP ESWAEPSAAE AAAESASKPK PRSRAIKH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac aaaaagaaaa gcattttgag gaatatgctg ctttggcgac ccttccttta cgggatgtcg tcgtttaccc gcatatggtt ctgccgctgt tcgtcggcag gccgaaatcc atcgccgcac tggaaaacgc cattacccgc gaggaaccgg ttttcctgtt ggcgcaaacc gatgcggcgg tagaagaacc ggttgccacc gacctgtatc agaccggtac cgtcgcacaa gtcctgcaag tgttgaaact gcccgacggc acggtaaaag tgctggtcga agggctgtat cgcggacgtg ttttgaccat cgaagacacg ggcgggctgt tcgtttctca tatagaggcg gtcgtggaag aagacacggg cggcaatacc gacctcgaag ccgtgcgccg caccctgttg gcgcagtttg aacaatacgc caaactcaat aaaaaaatcc ctgccgaaat tatcggcagc atcaacggca ttgccgaaaa cagccggcta accgatacgg tcgcagcgca tttgcagttg aaactggcgc aacgccaaca gattttggag attcccgaaa tcggcaaacg gatggaattc ctgctggcaa agctggaatc cgaactcgac attatgcagg ccgaaaaacg catacgcgga cgcgtcaaac gccaaatgga aaaatcccaa cgcgaatatt atctgaacga acagattaaa gcgatacaca aagaattggg cgaagaagac gaaaacggcg aactggatgc cttggaggca ggcatcaaaa aggcgggtat gaccaaagaa gcggaagaaa aatgcctgtc cgaactgaaa aaactcaaaa tgatgccgcc gatgtctgcc gaatccaccg tcgtacgcaa ctacatcgac accctgctcg gactgccgtg gaagaaaaaa tcccgcgtca gcaaagacat cgccaaagcc ggactggtgc tggatgccga ccactacggc ctggaaaaag tcaaagaacg gattttggaa tacctcgccg tccaaaaacg tatggacaag ctcaaaggtc cgattctgtg tttggtcggc cctccgggcg tgggcaaaac ctctttgggc gaatccatcg ccaaagcaac ggggcggaaa tatgtccgta tggccttggg cggcgtgcgc gacgaaagcg aaatccgcgg gcaccgccgt acctatatcg gctctatgcc cggcaagatt ttgcagaata tggcaaaagc aggcgtaaga aacccgttgt tcctgctcga cgaaatcgac aaattgggca gcgactttcg gggcgatccc gccagcgcgt tgcttgaagt gctcgatcac gaacaaaaca acaagttcgc cgatcattat gccgaagtgg attacgattt gagcgacgtg atgtttattg cgacttcaaa cagcctgaat atcccgaccc cgctgcttga ccgtatggaa atcatccgtc tgtccggcta taccgaagac gagaaaatca atatcgcgat gcagtatctc gtaccgaagc aaatgaaacg caacggcgtg aaagaaggcg aattggtggt cgaagaaagc gcggtacgcg atattatccg ttattacacc cgagaggcgg gcgtgcgttc gctcgaccgc gaaattgcca aaatctgccg taaggtggtg atgcagatta ccttgaacga agataagaag aggttgtctg aaaccaagaa aaccagcaaa gccaagccta gggcggttaa agtcaatgag aaaaacctac atgactactt gggcgtgcgc cgcttcgatt acggcgttgc cgaaagtgaa aaccgcatcg gacaggttac cggtttggct tggacggaag tcggcggcga attgctgact gtcgaagccg cagcattacc gggtaaaggc atgatccagt gtaccggcca gttgggcgat gtgatgaagg aatctgtatc cgctgcatgg tctgttgtcc gttcccgtgc ggaatcagta ggtttggctc ctgattttta cgagaaaaaa gacattcatg tccatgttcc cgaaggtgca acgccgaaag acggtccgag cgcaggcatt gctatgactt tggcgatggt gtccgccttt accaaaattc cggtgcgtgc cgatgttgcc atgacgggcg aaattaccct gcgcggcgaa gtcctgccga tcggcggtct gaaggaaaaa ctgttggctg cgcttcgcgg cggcatcaaa cacgtcctga ttccgaaaga caatgtcaaa gacttggaag aaatccctga aaacgtgaaa accggattaa ccatccatcc ggtcaaatgg atagacgagg tattggcttt gggtttggaa agccggcctg agtcttgggc agaaccttct gccgcggaag cggcggcgga atccgcttca aaaccgaaac cccgcagcag ggcaatcaaa cattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for NegoA.18178.a.B3.GE43100
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 65kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAI HKELGEEDEN GELDALEAGI KKAGMTKEAE EKCLSELKKL KMMPPMSAES TVVRNYIDTL LGLPWKKKSR VSKDIAKAGL VLDADHYGLE KVKERILEYL AVQKRMDKLK GPILCLVGPP GVGKTSLGES IAKATGRKYV RMALGGVRDE SEIRGHRRTY IGSMPGKILQ NMAKAGVRNP LFLLDEIDKL GSDFRGDPAS ALLEVLDHEQ NNKFADHYAE VDYDLSDVMF IATSNSLNIP TPLLDRMEII RLSGYTEDEK INIAMQYLVP KQMKRNGVKE GELVVEESAV RDIIRYYTRE AGVRSLDREI AKICRKVVMQ ITLNEDKKRL SETKKTSKAK PRAVKVNEKN LHDYLGVRRF DYGVAESENR IGQVTGLAWT EVGGELLTVE AAALPGKGMI QCTGQLGDVM KESVSAAWSV VRSRAESVGL APDFYEKKDI HVHVPEGATP KDGPSAGIAM TLAMVSAFTK IPVRADVAMT GEITLRGEVL PIGGLKEKLL AALRGGIKHV LIPKDNVKDL EEIPENVKTG LTIHPVKWID EVLALGLESR PESWAEPSAA EAAAESASKP KPRSRAIKH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgatac acaaagaatt gggcgaagaa gacgaaaacg gcgaactgga tgccttggag gcaggcatca aaaaggcggg tatgaccaaa gaagcggaag aaaaatgcct gtccgaactg aaaaaactca aaatgatgcc gccgatgtct gccgaatcca ccgtcgtacg caactacatc gacaccctgc tcggactgcc gtggaagaaa aaatcccgcg tcagcaaaga catcgccaaa gccggactgg tgctggatgc cgaccactac ggcctggaaa aagtcaaaga acggattttg gaatacctcg ccgtccaaaa acgtatggac aagctcaaag gtccgattct gtgtttggtc ggccctccgg gcgtgggcaa aacctctttg ggcgaatcca tcgccaaagc aacggggcgg aaatatgtcc gtatggcctt gggcggcgtg cgcgacgaaa gcgaaatccg cgggcaccgc cgtacctata tcggctctat gcccggcaag attttgcaga atatggcaaa agcaggcgta agaaacccgt tgttcctgct cgacgaaatc gacaaattgg gcagcgactt tcggggcgat cccgccagcg cgttgcttga agtgctcgat cacgaacaaa acaacaagtt cgccgatcat tatgccgaag tggattacga tttgagcgac gtgatgttta ttgcgacttc aaacagcctg aatatcccga ccccgctgct tgaccgtatg gaaatcatcc gtctgtccgg ctataccgaa gacgagaaaa tcaatatcgc gatgcagtat ctcgtaccga agcaaatgaa acgcaacggc gtgaaagaag gcgaattggt ggtcgaagaa agcgcggtac gcgatattat ccgttattac acccgagagg cgggcgtgcg ttcgctcgac cgcgaaattg ccaaaatctg ccgtaaggtg gtgatgcaga ttaccttgaa cgaagataag aagaggttgt ctgaaaccaa gaaaaccagc aaagccaagc ctagggcggt taaagtcaat gagaaaaacc tacatgacta cttgggcgtg cgccgcttcg attacggcgt tgccgaaagt gaaaaccgca tcggacaggt taccggtttg gcttggacgg aagtcggcgg cgaattgctg actgtcgaag ccgcagcatt accgggtaaa ggcatgatcc agtgtaccgg ccagttgggc gatgtgatga aggaatctgt atccgctgca tggtctgttg tccgttcccg tgcggaatca gtaggtttgg ctcctgattt ttacgagaaa aaagacattc atgtccatgt tcccgaaggt gcaacgccga aagacggtcc gagcgcaggc attgctatga ctttggcgat ggtgtccgcc tttaccaaaa ttccggtgcg tgccgatgtt gccatgacgg gcgaaattac cctgcgcggc gaagtcctgc cgatcggcgg tctgaaggaa aaactgttgg ctgcgcttcg cggcggcatc aaacacgtcc tgattccgaa agacaatgtc aaagacttgg aagaaatccc tgaaaacgtg aaaaccggat taaccatcca tccggtcaaa tggatagacg aggtattggc tttgggtttg gaaagccggc ctgagtcttg ggcagaacct tctgccgcgg aagcggcggc ggaatccgct tcaaaaccga aaccccgcag cagggcaatc aaacattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NegoA.18178.a.A3.PW38560
PURIFICATION DATe: 10/30/2018
CONCENTRATION: 10.43mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SAIHKELGEE DENGELDALE AGIKKAGMTK EAEEKCLSEL KKLKMMPPMS AESTVVRNYI DTLLGLPWKK KSRVSKDIAK AGLVLDADHY GLEKVKERIL EYLAVQKRMD KLKGPILCLV GPPGVGKTSL GESIAKATGR KYVRMALGGV RDESEIRGHR RTYIGSMPGK ILQNMAKAGV RNPLFLLDEI DKLGSDFRGD PASALLEVLD HEQNNKFADH YAEVDYDLSD VMFIATSNSL NIPTPLLDRM EIIRLSGYTE DEKINIAMQY LVPKQMKRNG VKEGELVVEE SAVRDIIRYY TREAGVRSLD REIAKICRKV VMQITLNEDK KRLSETKKTS KAKPRAVKVN EKNLHDYLGV RRFDYGVAES ENRIGQVTGL AWTEVGGELL TVEAAALPGK GMIQCTGQLG DVMKESVSAA WSVVRSRAES VGLAPDFYEK KDIHVHVPEG ATPKDGPSAG IAMTLAMVSA FTKIPVRADV AMTGEITLRG EVLPIGGLKE KLLAALRGGI KHVLIPKDNV KDLEEIPENV KTGLTIHPVK WIDEVLALGL ESRPESWAEP SAAEAAAESA SKPKPRSRAI KH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGC GATACACAAA GAATTGGGCG AAGAAGACGA AAACGGCGAA CTGGATGCCT TGGAGGCAGG CATCAAAAAG GCGGGTATGA CCAAAGAAGC GGAAGAAAAA TGCCTGTCCG AACTGAAAAA ACTCAAAATG ATGCCGCCGA TGTCTGCCGA ATCCACCGTC GTACGCAACT ACATCGACAC CCTGCTCGGA CTGCCGTGGA AGAAAAAATC CCGCGTCAGC AAAGACATCG CCAAAGCCGG ACTGGTGCTG GATGCCGACC ACTACGGCCT GGAAAAAGTC AAAGAACGGA TTTTGGAATA CCTCGCCGTC CAAAAACGTA TGGACAAGCT CAAAGGTCCG ATTCTGTGTT TGGTCGGCCC TCCGGGCGTG GGCAAAACCT CTTTGGGCGA ATCCATCGCC AAAGCAACGG GGCGGAAATA TGTCCGTATG GCCTTGGGCG GCGTGCGCGA CGAAAGCGAA ATCCGCGGGC ACCGCCGTAC CTATATCGGC TCTATGCCCG GCAAGATTTT GCAGAATATG GCAAAAGCAG GCGTAAGAAA CCCGTTGTTC CTGCTCGACG AAATCGACAA ATTGGGCAGC GACTTTCGGG GCGATCCCGC CAGCGCGTTG CTTGAAGTGC TCGATCACGA ACAAAACAAC AAGTTCGCCG ATCATTATGC CGAAGTGGAT TACGATTTGA GCGACGTGAT GTTTATTGCG ACTTCAAACA GCCTGAATAT CCCGACCCCG CTGCTTGACC GTATGGAAAT CATCCGTCTG TCCGGCTATA CCGAAGACGA GAAAATCAAT ATCGCGATGC AGTATCTCGT ACCGAAGCAA ATGAAACGCA ACGGCGTGAA AGAAGGCGAA TTGGTGGTCG AAGAAAGCGC GGTACGCGAT ATTATCCGTT ATTACACCCG AGAGGCGGGC GTGCGTTCGC TCGACCGCGA AATTGCCAAA ATCTGCCGTA AGGTGGTGAT GCAGATTACC TTGAACGAAG ATAAGAAGAG GTTGTCTGAA ACCAAGAAAA CCAGCAAAGC CAAGCCTAGG GCGGTTAAAG TCAATGAGAA AAACCTACAT GACTACTTGG GCGTGCGCCG CTTCGATTAC GGCGTTGCCG AAAGTGAAAA CCGCATCGGA CAGGTTACCG GTTTGGCTTG GACGGAAGTC GGCGGCGAAT TGCTGACTGT CGAAGCCGCA GCATTACCGG GTAAAGGCAT GATCCAGTGT ACCGGCCAGT TGGGCGATGT GATGAAGGAA TCTGTATCCG CTGCATGGTC TGTTGTCCGT TCCCGTGCGG AATCAGTAGG TTTGGCTCCT GATTTTTACG AGAAAAAAGA CATTCATGTC CATGTTCCCG AAGGTGCAAC GCCGAAAGAC GGTCCGAGCG CAGGCATTGC TATGACTTTG GCGATGGTGT CCGCCTTTAC CAAAATTCCG GTGCGTGCCG ATGTTGCCAT GACGGGCGAA ATTACCCTGC GCGGCGAAGT CCTGCCGATC GGCGGTCTGA AGGAAAAACT GTTGGCTGCG CTTCGCGGCG GCATCAAACA CGTCCTGATT CCGAAAGACA ATGTCAAAGA CTTGGAAGAA ATCCCTGAAA ACGTGAAAAC CGGATTAACC ATCCATCCGG TCAAATGGAT AGACGAGGTA TTGGCTTTGG GTTTGGAAAG CCGGCCTGAG TCTTGGGCAG AACCTTCTGC CGCGGAAGCG GCGGCGGAAT CCGCTTCAAA ACCGAAACCC CGCAGCAGGG CAATCAAACA TTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA