CENTER ID: | NegoA.18178.a |
ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
ASSOCIATED DISEASE: | |
CURRENT STATUS: | purified |
COMMUNITY REQUEST: | True |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
I/II |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
NegoA.18178.a.A3.GE42461 | Truncation 2 | 240 | 820 | ||
NegoA.18178.a.A4.GE42462 | Truncation 3 | 252 | 820 | ||
NegoA.18178.a.B1.GE40247 | full length | 1 | 820 | ||
NegoA.18178.a.B3.GE43100 | Truncation 2 | 240 | 820 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
NegoA.18178.a.A3.PW38560 | Truncation 2 | 240 | 820 |
METHOD | RESULTS |
---|---|
comparative modelling | Robetta_85051 |
RESOURCE | REFERENCE ID |
---|---|
PATRIC ID: | fig|521006.8.peg.1184 |
UniProt: | B4RLU1 |
HARVESTED ON: | 5/25/2018 |
SEQUENCED ON: | 5/31/2018 |
EXPECTED MW: | 66kDa |
OBSERVED MW: | 66kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 5/25/2018 |
SEQUENCED ON: | 5/31/2018 |
EXPECTED MW: | 65kDa |
OBSERVED MW: | 65kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Moderate Expression |
SOLUBLE EXPRESSION LEVEL | Moderate Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 5/9/2016 |
SEQUENCED ON: | 5/23/2016 |
EXPECTED MW: | 92kDa |
OBSERVED MW: | 90kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Low Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
HARVESTED ON: | 4/19/2019 |
SEQUENCED ON: | 4/23/2019 |
EXPECTED MW: | 65kDa |
OBSERVED MW: | 65kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Low Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |
PURIFICATION DATe: | 10/30/2018 |
CONCENTRATION: | 10.43mg/ml |
OBSERVED MW: | 66kDa |
EXPRESSION LEVEL: | Low Expression |
PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
VIAL COUNT (approx.): | 8 |
VIAL VOLUME: | 200µl |
PERCENT IDENTITY: | data unavailable |
PERCENT COVERAGE: | data unavailable |