NegoA.18161.a
ribulose-phosphate 3-epimerase
| CENTER ID: | NegoA.18161.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.18161.a.B1.GE40246 | full length | 1 | 227 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.18161.a.B1.PS38017 | full length | 1 | 227 |
Structures
5UMF
DEPOSITED: 1/27/2017
DETERMINATION: XRay
CLONE: NegoA.18161.a.B1.GE40246
PROTEIN: NegoA.18161.a.B1.PS38017
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.1201 |
| UniProt: | B4RLV9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAYRIAPSI LSADFARLGE EVANVIAAGA DLIHFDVMDN HYVPNLTFGP MVCAALKPYA SVPIDVHLMV EPVDDLIQSF AKAGASIITF HPEASRHIDR SLSLIRDMGC QAGLVLNPAT PVYVLENVLD RLDMVLLMSV NPGFGGQSFI PHTLEKIRQV RAMLDRYEGK SGRRIAIEVD GGIKTDNIAA VARAGADTFV AGSAIFGKPD YKAVIAAMRA ELEKAAG
NT Sequence
ATGACCGCCT ACCGTATCGC ACCCAGTATT TTGTCGGCAG ACTTTGCCCG CCTCGGAGAA GAGGTGGCAA ATGTCATTGC GGCGGGTGCG GATCTGATCC ATTTTGACGT GATGGACAAC CATTATGTGC CGAACCTGAC CTTCGGACCT ATGGTCTGCG CGGCGTTGAA GCCTTATGCA AGCGTGCCGA TCGATGTGCA TCTGATGGTC GAACCCGTTG ACGATTTAAT TCAATCGTTT GCCAAAGCAG GAGCATCGAT TATCACTTTC CATCCCGAAG CGAGCCGCCA TATCGACCGC AGCTTGAGCC TGATTCGGGA CATGGGCTGT CAGGCGGGGC TGGTGTTGAA TCCGGCAACG CCCGTATATG TGTTGGAAAA CGTGTTGGAC AGGCTGGATA TGGTTTTGCT GATGTCGGTC AACCCCGGGT TCGGCGGTCA GAGCTTCATT CCGCACACTT TGGAAAAAAT CAGACAGGTT CGCGCCATGC TTGACCGCTA TGAAGGGAAA AGCGGCAGAC GTATTGCCAT TGAAGTGGAC GGCGGCATCA AAACTGACAA TATTGCCGCC GTTGCCCGAG CCGGTGCGGA TACCTTTGTT GCCGGTTCGG CGATTTTCGG CAAACCGGAT TACAAGGCGG TAATCGCTGC GATGAGGGCG GAACTGGAAA AAGCGGCAGG CTGA
Details for NegoA.18161.a.B1.GE40246
| HARVESTED ON: | 5/9/2016 |
| SEQUENCED ON: | 5/23/2016 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT AYRIAPSILS ADFARLGEEV ANVIAAGADL IHFDVMDNHY VPNLTFGPMV CAALKPYASV PIDVHLMVEP VDDLIQSFAK AGASIITFHP EASRHIDRSL SLIRDMGCQA GLVLNPATPV YVLENVLDRL DMVLLMSVNP GFGGQSFIPH TLEKIRQVRA MLDRYEGKSG RRIAIEVDGG IKTDNIAAVA RAGADTFVAG SAIFGKPDYK AVIAAMXXEL EKAAG
Validated NT Sequence
atcttactca cttagcctgc cgctttttcc agttccgnnn tcatcgcagc gattaccgcc ttgtaatccg gtttgccgaa aatcgccgaa ccggcaacaa aggtatccgc accggctcgg gcaacggcgg caatattgtc agttttgatg ccgccgtcca cttcaatggc aatacgtctg ccgcttttcc cttcatagcg gtcaagcatg gcgcgaacct gtctgatttt ttccaaagtg tgcggaatga agctctgacc gccgaacccg gggttgaccg acatcagcaa aaccatatcc agcctgtcta acacgttttc caacacatat acgggcgttg ccggattcaa caccagcccc gcctgacagc ccatgtcccg aatcaggctc aagctgcggt cgatatggcg gctcgcttcg ggatggaaag tgataatcga tgctcctgct ttggcaaacg attgaattaa atcgtcaacg ggttcgacca tcagatgcac atcgatcggc acgcttgcat aaggcttcaa cgccgcgcag accataggtc cgaaggtcag gttcggcaca taatggttgt ccatcacgtc aaaatggatc agatccgcac ccgccgcaat gacatttgcc acctcttctc cgaggcgggc aaagtctgcc gacaaaatac tgggtgcgat acggtaggcg gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT AYRIAPSILS ADFARLGEEV ANVIAAGADL IHFDVMDNHY VPNLTFGPMV CAALKPYASV PIDVHLMVEP VDDLIQSFAK AGASIITFHP EASRHIDRSL SLIRDMGCQA GLVLNPATPV YVLENVLDRL DMVLLMSVNP GFGGQSFIPH TLEKIRQVRA MLDRYEGKSG RRIAIEVDGG IKTDNIAAVA RAGADTFVAG SAIFGKPDYK AVIAAMRAEL EKAAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg cctaccgtat cgcacccagt attttgtcgg cagactttgc ccgcctcgga gaagaggtgg caaatgtcat tgcggcgggt gcggatctga tccattttga cgtgatggac aaccattatg tgccgaacct gaccttcgga cctatggtct gcgcggcgtt gaagccttat gcaagcgtgc cgatcgatgt gcatctgatg gtcgaacccg ttgacgattt aattcaatcg tttgccaaag caggagcatc gattatcact ttccatcccg aagcgagccg ccatatcgac cgcagcttga gcctgattcg ggacatgggc tgtcaggcgg ggctggtgtt gaatccggca acgcccgtat atgtgttgga aaacgtgttg gacaggctgg atatggtttt gctgatgtcg gtcaaccccg ggttcggcgg tcagagcttc attccgcaca ctttggaaaa aatcagacag gttcgcgcca tgcttgaccg ctatgaaggg aaaagcggca gacgtattgc cattgaagtg gacggcggca tcaaaactga caatattgcc gccgttgccc gagccggtgc ggataccttt gttgccggtt cggcgatttt cggcaaaccg gattacaagg cggtaatcgc tgcgatgagg gcggaactgg aaaaagcggc aggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for NegoA.18161.a.B1.PS38017
| PURIFICATION DATe: | 8/16/2016 |
| CONCENTRATION: | 26.22mg/ml |
| OBSERVED MW: | 25kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT AYRIAPSILS ADFARLGEEV ANVIAAGADL IHFDVMDNHY VPNLTFGPMV CAALKPYASV PIDVHLMVEP VDDLIQSFAK AGASIITFHP EASRHIDRSL SLIRDMGCQA GLVLNPATPV YVLENVLDRL DMVLLMSVNP GFGGQSFIPH TLEKIRQVRA MLDRYEGKSG RRIAIEVDGG IKTDNIAAVA RAGADTFVAG SAIFGKPDYK AVIAAMXXEL EKAAG
Validated NT Sequence
atcttactca cttagcctgc cgctttttcc agttccgnnn tcatcgcagc gattaccgcc ttgtaatccg gtttgccgaa aatcgccgaa ccggcaacaa aggtatccgc accggctcgg gcaacggcgg caatattgtc agttttgatg ccgccgtcca cttcaatggc aatacgtctg ccgcttttcc cttcatagcg gtcaagcatg gcgcgaacct gtctgatttt ttccaaagtg tgcggaatga agctctgacc gccgaacccg gggttgaccg acatcagcaa aaccatatcc agcctgtcta acacgttttc caacacatat acgggcgttg ccggattcaa caccagcccc gcctgacagc ccatgtcccg aatcaggctc aagctgcggt cgatatggcg gctcgcttcg ggatggaaag tgataatcga tgctcctgct ttggcaaacg attgaattaa atcgtcaacg ggttcgacca tcagatgcac atcgatcggc acgcttgcat aaggcttcaa cgccgcgcag accataggtc cgaaggtcag gttcggcaca taatggttgt ccatcacgtc aaaatggatc agatccgcac ccgccgcaat gacatttgcc acctcttctc cgaggcgggc aaagtctgcc gacaaaatac tgggtgcgat acggtaggcg gtcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMT AYRIAPSILS ADFARLGEEV ANVIAAGADL IHFDVMDNHY VPNLTFGPMV CAALKPYASV PIDVHLMVEP VDDLIQSFAK AGASIITFHP EASRHIDRSL SLIRDMGCQA GLVLNPATPV YVLENVLDRL DMVLLMSVNP GFGGQSFIPH TLEKIRQVRA MLDRYEGKSG RRIAIEVDGG IKTDNIAAVA RAGADTFVAG SAIFGKPDYK AVIAAMRAEL EKAAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG CCTACCGTAT CGCACCCAGT ATTTTGTCGG CAGACTTTGC CCGCCTCGGA GAAGAGGTGG CAAATGTCAT TGCGGCGGGT GCGGATCTGA TCCATTTTGA CGTGATGGAC AACCATTATG TGCCGAACCT GACCTTCGGA CCTATGGTCT GCGCGGCGTT GAAGCCTTAT GCAAGCGTGC CGATCGATGT GCATCTGATG GTCGAACCCG TTGACGATTT AATTCAATCG TTTGCCAAAG CAGGAGCATC GATTATCACT TTCCATCCCG AAGCGAGCCG CCATATCGAC CGCAGCTTGA GCCTGATTCG GGACATGGGC TGTCAGGCGG GGCTGGTGTT GAATCCGGCA ACGCCCGTAT ATGTGTTGGA AAACGTGTTG GACAGGCTGG ATATGGTTTT GCTGATGTCG GTCAACCCCG GGTTCGGCGG TCAGAGCTTC ATTCCGCACA CTTTGGAAAA AATCAGACAG GTTCGCGCCA TGCTTGACCG CTATGAAGGG AAAAGCGGCA GACGTATTGC CATTGAAGTG GACGGCGGCA TCAAAACTGA CAATATTGCC GCCGTTGCCC GAGCCGGTGC GGATACCTTT GTTGCCGGTT CGGCGATTTT CGGCAAACCG GATTACAAGG CGGTAATCGC TGCGATGAGG GCGGAACTGG AAAAAGCGGC AGGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT