SSGCID: Target Page

NegoA.18149.a

amidophosphoribosyltransferase

CENTER ID:	NegoA.18149.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.18149.a.B1.GE40245	full length		1	514

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.18149.a.B1.PS38046	full length		1	514

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.451
UniProt:	B4RJU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MCGVLGLVSH EPVNQLLYDG LQMLQHRGQD AAGIATAEGG TFHMHKGKGM VSEVFRTRNM RDLTGNAGIA HVRYPTAGNA GSSAEAQPFY VSSPFGIVLA HNGNLTNTAE LYENVCNKHL RHINTGSDSE VLLNVFAHEL RREVSKNADP HRLNADNIFN AVAEVHRLVR GAYGVVAMIA GYGMVAFRDP YGIRPLALGS QTDSEGRKSY AVASESVAFN ALAYDLERDI RPGEAVFVGF NGTIIARQCS DRAKLSPCLF EYVYFARPDS VIDGVSVYQS RLDMGVSLAE KIKRELPVDG IDVVMPIPDT SRPSAMELAV HLNKPYREGL IKNRYIGRTF IMPGQSTRKK SVRQKLSPME TEFAGKSVLL VDDSIVRGTT SREIVEMVRA AGARKVYIAS AAPEVRYPNV YGIDMPTREE LIANGRSAAE IAAEIGADGI VFQDLGDLEA VVKALNPKIE SFDSSCFNGI YRTGDIDDAY LDRLSAEKSG CAGLKIHPSR MEHSISISDA GDEE

NT Sequence

ATGTGCGGCG TATTAGGTTT GGTCAGTCAT GAACCCGTGA ACCAGCTTCT GTACGACGGT TTGCAGATGT TGCAGCACAG GGGGCAGGAT GCGGCGGGCA TTGCAACGGC GGAAGGCGGT ACCTTCCATA TGCACAAAGG CAAAGGGATG GTGAGCGAAG TGTTCCGCAC GCGCAATATG CGCGATTTGA CCGGCAACGC CGGCATCGCC CACGTCCGTT ATCCCACGGC GGGCAACGCC GGCAGCAGCG CGGAGGCGCA GCCTTTCTAC GTCAGCTCGC CTTTCGGCAT CGTTTTGGCG CACAACGGCA ACCTCACCAA CACTGCCGAA CTGTATGAAA ACGTGTGTAA CAAACACCTG CGCCACATCA ACACCGGCTC CGATTCCGAA GTTTTGCTCA ACGTATTCGC GCACGAATTG CGCCGCGAAG TCTCTAAAAA CGCCGACCCG CACCGGCTCA ATGCCGACAA TATTTTCAAC GCCGTTGCCG AAGTCCACCG CCTGGTGCGC GGTGCATACG GCGTGGTTGC CATGATTGCG GGCTACGGCA TGGTTGCCTT CCGTGATCCT TACGGCATCC GCCCGCTGGC ATTGGGTTCG CAAACCGACA GCGAAGGCAG GAAATCCTAT GCCGTCGCCT CCGAATCCGT CGCCTTCAAC GCGCTTGCCT ACGATTTGGA ACGCGATATC CGGCCGGGCG AAGCGGTATT TGTCGGCTTT AACGGCACAA TCATCGCCCG ACAATGCAGC GACCGCGCCA AACTCAGCCC CTGCCTTTTT GAATATGTTT ATTTTGCCCG CCCCGACTCT GTGATTGACG GCGTGTCCGT TTACCAGTCG CGCTTGGATA TGGGCGTGTC CTTGGCGGAA AAAATCAAAC GCGAGCTGCC CGTGGACGGC ATCGACGTCG TGATGCCCAT TCCCGATACC AGCCGCCCCA GTGCAATGGA GCTTGCCGTC CACCTCAACA AACCCTACCG CGAGGGTTTG ATTAAAAACC GCTATATCGG CCGCACCTTT ATCATGCCCG GTCAGTCGAC GCGCAAAAAA TCCGTGCGCC AAAAACTCAG CCCGATGGAA ACCGAATTTG CAGGCAAAAG CGTGTTGCTG GTGGACGACT CCATCGTGCG CGGGACGACC AGCCGCGAAA TCGTCGAAAT GGTACGCGCG GCGGGCGCGC GCAAAGTCTA TATCGCCTCC GCCGCGCCCG AAGTGCGCTA TCCCAATGTG TACGGCATCG ATATGCCCAC GCGCGAAGAG TTGATTGCCA ACGGGCGCAG CGCGGCGGAA ATCGCCGCCG AAATCGGCGC GGACGGCATC GTGTTTCAAG ACTTGGGCGA TTTGGAAGCC GTCGTCAAAG CACTCAACCC GAAAATCGAA TCCTTCGATT CGTCCTGTTT CAACGGCATT TATCGAACCG GCGACATCGA TGATGCCTAC CTCGACCGCC TGTCTGCCGA GAAATCCGGC TGCGCCGGTC TGAAAATCCA CCCGAGCAGG ATGGAACACA GCATCAGCAT CAGCGATGCG GGCGACGAAG AATAA

Details for NegoA.18149.a.B1.GE40245

HARVESTED ON:	5/9/2016
SEQUENCED ON:	5/23/2016
EXPECTED MW:	57kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMC GVLGLVSHEP VNQLLYDGLQ MLQHRGQDAA GIATAEGGTF HMHKGKGMVS EVFRTRNMRD LTGNAGIAHV RYPTAGNAGS SAEAQPFYVS SPFGIVLAHN GNLTNTAELY ENVCNKHLRH INTGSDSEVL LNVFAHELRR EVSKNADPHR LNADNIFNAV AEVHRLVRGA YGVVAMIAGY GMLAFRDPYG IRPLALGSQT DSEGRKSYAV ASESVAFNAL AYDLERDIRP GEAVFVGFNG TIIARQCSDR AKLXXXLFEY VYFARPDSVI DGVSVYQSRL DMGVSLAEKI KRELPVDGID VVMPIPDTSR PSAMELAVHL NKPYREGLIK NRYIGRTFIM PGQSTRKKSV RQKLSPMETE FAGKSVLLVD DSIVRGTTSR EIVEMVRAAG ARKVYIASAA PEVRYPNVYG IDMPTREELI ANGRSAAEIA AEIGADGIVF QDLGDLEAVV KALNPKIESF DSSCFNGIYR TGDIDDAYLD RLSAEKSGCA GLKIHPSRME HSIS

Validated NT Sequence

tgctgatgct gtgttccatc ctgctcgggt ggattttcag accggcgcag ccggatttct cggcagacag gcggtcgagg taggcatcat cgatgtcgcc ggtccgataa atgccgttga aacaggacga atcgaaggat tcgattttcg ggttgagtgc tttgacgacg gcttccaaat cgcccaagtc ttgaaacacg atgccgtccg cgccgatttc ggcggcgatt tccgccgcgc tgcgcccgtt ggcaatcaac tcttcgcgcg tgggcatatc gatgccgtac acattgggat agcgcacttc gggcgcggcg gaggcgatat agactttgcg cgcgcccgcc gcgcgtacca tttcgacgat ttcgcggctg gtcgtcccgc gcacgatgga gtcgtccacc agcaacacgc ttttgcctgc aaattcggtt tccatcgggc tgagtttttg gcgcacggat tttttgcgcg tcgactgacc gggcataata aaggtgcggc cgatatagcg gtttttaatc aaaccctcgc ggtagggttt gttgaggtgg acggcaagct ccattgcact ggggcggctg gtatcgggaa tgggcatcac gacgtcgatg ccgtccacgg gcagctcgcg tttgattttt tccgccaagg acacgcccat atccaagcgc gattggtaaa ccgatacacc gtcaatcaca gagtcggggc gggcaaaata aacatattca aaaaggcngn nnntgagttt ggcacggtcg ctgcattgtc gggcgatgat tgtgccgtta aagccgacaa ataccgcttc gcccggccgg atatcgcgtt ccaaatcgta ggcaagcgca ttgaaggcga cggattcgga ggcgacggca taggatttcc tgccttcgct gtcggtttgc gaacccaatg ccagcgggcg gatgccgtaa gggtcgcgga aggcgagcat accgtagccc gcaatcatgg caaccacgcc gtatgcaccg cgcactaggc ggtggacttc ggcaacggcg ttgaaaatat tgtcggcatt gagccggtgc gggtcggcgt ttttagagac ttcgcggcgc aattcgtgcg cgaatacgtt gagcaaaact tcggaatcgg agccggtgtt gatgtggcgc aggtgtttgt tacacacgtt ttcatacagt tcggcagtgt tggtgaggtt gccgttgtgc gccaaaacga tgccgaaagg cgagctgacg tagaaaggct gcgcctccgc gctgctgccg gcgttgcccg ccgtgggata acggacgtgg gcgatgccgg cgttgccggt caaatcgcgc atattgcgcg tgcggaacac ttcgctcacc atccctttgc ctttgtgcat atggaaggta ccgccttccg ccgttgcaat gcccgccgca tcctgccccc tgtgctgcaa catctgcaaa ccgtcgtaca gaagctggtt cacgggttca tgactgacca aacctaatac gccgcacata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMC GVLGLVSHEP VNQLLYDGLQ MLQHRGQDAA GIATAEGGTF HMHKGKGMVS EVFRTRNMRD LTGNAGIAHV RYPTAGNAGS SAEAQPFYVS SPFGIVLAHN GNLTNTAELY ENVCNKHLRH INTGSDSEVL LNVFAHELRR EVSKNADPHR LNADNIFNAV AEVHRLVRGA YGVVAMIAGY GMVAFRDPYG IRPLALGSQT DSEGRKSYAV ASESVAFNAL AYDLERDIRP GEAVFVGFNG TIIARQCSDR AKLSPCLFEY VYFARPDSVI DGVSVYQSRL DMGVSLAEKI KRELPVDGID VVMPIPDTSR PSAMELAVHL NKPYREGLIK NRYIGRTFIM PGQSTRKKSV RQKLSPMETE FAGKSVLLVD DSIVRGTTSR EIVEMVRAAG ARKVYIASAA PEVRYPNVYG IDMPTREELI ANGRSAAEIA AEIGADGIVF QDLGDLEAVV KALNPKIESF DSSCFNGIYR TGDIDDAYLD RLSAEKSGCA GLKIHPSRME HSISISDAGD EE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgcg gcgtattagg tttggtcagt catgaacccg tgaaccagct tctgtacgac ggtttgcaga tgttgcagca cagggggcag gatgcggcgg gcattgcaac ggcggaaggc ggtaccttcc atatgcacaa aggcaaaggg atggtgagcg aagtgttccg cacgcgcaat atgcgcgatt tgaccggcaa cgccggcatc gcccacgtcc gttatcccac ggcgggcaac gccggcagca gcgcggaggc gcagcctttc tacgtcagct cgcctttcgg catcgttttg gcgcacaacg gcaacctcac caacactgcc gaactgtatg aaaacgtgtg taacaaacac ctgcgccaca tcaacaccgg ctccgattcc gaagttttgc tcaacgtatt cgcgcacgaa ttgcgccgcg aagtctctaa aaacgccgac ccgcaccggc tcaatgccga caatattttc aacgccgttg ccgaagtcca ccgcctggtg cgcggtgcat acggcgtggt tgccatgatt gcgggctacg gcatggttgc cttccgtgat ccttacggca tccgcccgct ggcattgggt tcgcaaaccg acagcgaagg caggaaatcc tatgccgtcg cctccgaatc cgtcgccttc aacgcgcttg cctacgattt ggaacgcgat atccggccgg gcgaagcggt atttgtcggc tttaacggca caatcatcgc ccgacaatgc agcgaccgcg ccaaactcag cccctgcctt tttgaatatg tttattttgc ccgccccgac tctgtgattg acggcgtgtc cgtttaccag tcgcgcttgg atatgggcgt gtccttggcg gaaaaaatca aacgcgagct gcccgtggac ggcatcgacg tcgtgatgcc cattcccgat accagccgcc ccagtgcaat ggagcttgcc gtccacctca acaaacccta ccgcgagggt ttgattaaaa accgctatat cggccgcacc tttatcatgc ccggtcagtc gacgcgcaaa aaatccgtgc gccaaaaact cagcccgatg gaaaccgaat ttgcaggcaa aagcgtgttg ctggtggacg actccatcgt gcgcgggacg accagccgcg aaatcgtcga aatggtacgc gcggcgggcg cgcgcaaagt ctatatcgcc tccgccgcgc ccgaagtgcg ctatcccaat gtgtacggca tcgatatgcc cacgcgcgaa gagttgattg ccaacgggcg cagcgcggcg gaaatcgccg ccgaaatcgg cgcggacggc atcgtgtttc aagacttggg cgatttggaa gccgtcgtca aagcactcaa cccgaaaatc gaatccttcg attcgtcctg tttcaacggc atttatcgaa ccggcgacat cgatgatgcc tacctcgacc gcctgtctgc cgagaaatcc ggctgcgccg gtctgaaaat ccacccgagc aggatggaac acagcatcag catcagcgat gcgggcgacg aagaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for NegoA.18149.a.B1.PS38046

PURIFICATION DATe:	9/7/2016
CONCENTRATION:	6.98mg/ml
OBSERVED MW:	58kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMC GVLGLVSHEP VNQLLYDGLQ MLQHRGQDAA GIATAEGGTF HMHKGKGMVS EVFRTRNMRD LTGNAGIAHV RYPTAGNAGS SAEAQPFYVS SPFGIVLAHN GNLTNTAELY ENVCNKHLRH INTGSDSEVL LNVFAHELRR EVSKNADPHR LNADNIFNAV AEVHRLVRGA YGVVAMIAGY GMVAFRDPYG IRPLALGSQT DSEGRKSYAV ASESVAFNAL AYDLERDIRP GEAVFVGFNG TIIARQCSDR AKLSPCLFEY VYFARPDSVI DGVSVYQSRL DMGVSLAEKI KRELPVDGID VVMPIPDTSR PSAMELAVHL NKPYREGLIK NRYIGRTFIM PGQSTRKKSV RQKLSPMETE FAGKSVLLVD DSIVRGTTSR EIVEMVRAAG ARKVYIASAA PEVRYPNVYG IDMPTREELI ANGRSAAEIA AEIGADGIVF QDLGDLEAVV KALNPKIESF DSSCFNGIYR TGDIDDAYLD RLSAEKSGCA GLKIHPSRME HSISISDAGD EE

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTGCG GCGTATTAGG TTTGGTCAGT CATGAACCCG TGAACCAGCT TCTGTACGAC GGTTTGCAGA TGTTGCAGCA CAGGGGGCAG GATGCGGCGG GCATTGCAAC GGCGGAAGGC GGTACCTTCC ATATGCACAA AGGCAAAGGG ATGGTGAGCG AAGTGTTCCG CACGCGCAAT ATGCGCGATT TGACCGGCAA CGCCGGCATC GCCCACGTCC GTTATCCCAC GGCGGGCAAC GCCGGCAGCA GCGCGGAGGC GCAGCCTTTC TACGTCAGCT CGCCTTTCGG CATCGTTTTG GCGCACAACG GCAACCTCAC CAACACTGCC GAACTGTATG AAAACGTGTG TAACAAACAC CTGCGCCACA TCAACACCGG CTCCGATTCC GAAGTTTTGC TCAACGTATT CGCGCACGAA TTGCGCCGCG AAGTCTCTAA AAACGCCGAC CCGCACCGGC TCAATGCCGA CAATATTTTC AACGCCGTTG CCGAAGTCCA CCGCCTGGTG CGCGGTGCAT ACGGCGTGGT TGCCATGATT GCGGGCTACG GCATGGTTGC CTTCCGTGAT CCTTACGGCA TCCGCCCGCT GGCATTGGGT TCGCAAACCG ACAGCGAAGG CAGGAAATCC TATGCCGTCG CCTCCGAATC CGTCGCCTTC AACGCGCTTG CCTACGATTT GGAACGCGAT ATCCGGCCGG GCGAAGCGGT ATTTGTCGGC TTTAACGGCA CAATCATCGC CCGACAATGC AGCGACCGCG CCAAACTCAG CCCCTGCCTT TTTGAATATG TTTATTTTGC CCGCCCCGAC TCTGTGATTG ACGGCGTGTC CGTTTACCAG TCGCGCTTGG ATATGGGCGT GTCCTTGGCG GAAAAAATCA AACGCGAGCT GCCCGTGGAC GGCATCGACG TCGTGATGCC CATTCCCGAT ACCAGCCGCC CCAGTGCAAT GGAGCTTGCC GTCCACCTCA ACAAACCCTA CCGCGAGGGT TTGATTAAAA ACCGCTATAT CGGCCGCACC TTTATCATGC CCGGTCAGTC GACGCGCAAA AAATCCGTGC GCCAAAAACT CAGCCCGATG GAAACCGAAT TTGCAGGCAA AAGCGTGTTG CTGGTGGACG ACTCCATCGT GCGCGGGACG ACCAGCCGCG AAATCGTCGA AATGGTACGC GCGGCGGGCG CGCGCAAAGT CTATATCGCC TCCGCCGCGC CCGAAGTGCG CTATCCCAAT GTGTACGGCA TCGATATGCC CACGCGCGAA GAGTTGATTG CCAACGGGCG CAGCGCGGCG GAAATCGCCG CCGAAATCGG CGCGGACGGC ATCGTGTTTC AAGACTTGGG CGATTTGGAA GCCGTCGTCA AAGCACTCAA CCCGAAAATC GAATCCTTCG ATTCGTCCTG TTTCAACGGC ATTTATCGAA CCGGCGACAT CGATGATGCC TACCTCGACC GCCTGTCTGC CGAGAAATCC GGCTGCGCCG GTCTGAAAAT CCACCCGAGC AGGATGGAAC ACAGCATCAG CATCAGCGAT GCGGGCGACG AAGAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT