NegoA.17999.a

adenylosuccinate lyase

CENTER ID: NegoA.17999.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17999.a.B1.GE40244 full length 1 456
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17999.a.B1.PS38036 full length 1 456

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.2256
UniProt: B4RPE0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MINPIASLSP LDGRYAQSVE ALRPIFSEYG LMKARVKVEL NWLKALAAEP EIAEVPPFSA ETLAEIDKVI ENFSLEDAAA VKAIEATTNH DVKAIEYWLK KRFAEVPEVA AVSEFIHFAC TSEDINNLSH ALMLQEARET VILPKLAEII GKLTAMAHDL AAVPMMSRTH GQPATPTTLG KETANVVYRL QRQFKILQAQ EFLGKINGAV GNYNAHMAAY PDVDWETHCR NFVEISLGLT FNPYTIQIEP HDYMAEFFQT LSRINTILID FSRDVWGYIS LGYFKQKVKA GEVGSSTMPH KVNPIDFENS EGNLGMANAV LGFLAEKLPV SRWQRDLTDS TVLRNMGVGV GYAVLGFAAH LRGLNKLEPN PAALAADLDV TWELLAEPIQ TVMRRYGVAN PYEKLKDLTH GKDGITPEVL KLFIESLEIP AEAKAKLLEL TPALYVGKAE ALAKRI
NT Sequence
ATGATCAACC CCATCGCCTC GCTTTCCCCC CTAGACGGCC GTTATGCCCA ATCCGTTGAA GCATTGCGCC CGATTTTTTC AGAGTACGGA CTGATGAAGG CGCGCGTCAA AGTCGAATTA AACTGGCTCA AAGCCCTGGC CGCCGAGCCG GAGATTGCCG AAGTGCCGCC CTTCAGTGCC GAAACGCTTG CCGAAATCGA CAAAGTCATC GAAAACTTTT CATTAGAAGA CGCGGCCGCC GTCAAAGCCA TCGAAGCCAC GACCAATCAT GATGTCAAAG CCATCGAATA TTGGCTGAAA AAACGTTTTG CCGAAGTGCC GGAAGTCGCC GCCGTGAGCG AGTTCATCCA CTTCGCCTGC ACCAGCGAAG ACATCAACAA CCTGTCCCAC GCCCTGATGC TGCAAGAGGC GCGCGAAACC GTCATCCTGC CCAAACTTGC CGAAATCATC GGCAAACTCA CCGCTATGGC GCACGACCTT GCCGCCGTCC CGATGATGAG CCGCACCCAC GGCCAGCCCG CCACACCGAC CACTTTGGGC AAAGAAACCG CCAATGTCGT GTACCGCCTG CAACGCCAGT TCAAAATCCT TCAGGCGCAA GAATTCCTCG GCAAAATCAA CGGCGCGGTC GGCAACTACA ACGCCCACAT GGCCGCCTAT CCCGATGTGG ATTGGGAAAC ACACTGCCGC AACTTCGTCG AAATCAGCCT CGGTCTGACC TTCAACCCCT ACACCATCCA AATCGAGCCG CACGACTATA TGGCGGAGTT TTTCCAAACC CTCAGCCGCA TCAACACGAT TCTCATCGAC TTCAGCCGCG ACGTTTGGGG TTATATTTCA TTGGGTTATT TCAAACAAAA AGTCAAAGCG GGCGAAGTCG GCTCTTCCAC CATGCCGCAC AAAGTCAACC CCATCGACTT TGAAAACTCC GAGGGCAACC TCGGCATGGC AAACGCCGTA TTGGGCTTTT TGGCGGAAAA ACTGCCCGTC TCACGCTGGC AGCGCGATTT GACCGACAGC ACCGTATTGC GCAATATGGG CGTGGGCGTG GGCTATGCCG TGTTGGGTTT CGCCGCCCAC CTGCGCGGTC TGAACAAGCT CGAACCCAAC CCTGCCGCGC TTGCCGCCGA TTTGGATGTC ACTTGGGAGC TGCTCGCCGA GCCGATTCAA ACCGTAATGC GCCGTTACGG TGTCGCCAAT CCTTACGAGA AGCTGAAAGA CCTGACGCAC GGTAAAGACG GCATTACGCC CGAAGTGTTG AAACTCTTTA TCGAATCGTT GGAAATTCCG GCAGAAGCCA AAGCCAAATT GCTTGAGCTG ACCCCTGCGT TGTATGTGGG CAAGGCTGAA GCGTTGGCGA AACGGATTTG A
Details for NegoA.17999.a.B1.GE40244
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI NPIASLSPLD GRYAQSVEAL RPIFSEYGLM KARVKVELNW LKALAAEPEI AEVPPFSAET LAEIDKVIEN FSLEDAAAVK AIEATTNHDV KAIEYWLKKR FAEVPEVAAV SEFIHFACTS EDINNLSHAL MLQEARETVI LPKLAEIIGK LTAMAHDLAA VPMMSRTHGQ PATPTTLGKE TANVVYRLQR QFKILQAQEF LGKINGAVGN YNAHMAAYPD VDWETHCRNF VEISLGLTFN PYTIQIEPHD YMAEFFQTLS RINTILIDFS RDVWGYISLG YFKQKVKAGE VGSSTMPHKV NPIDFENSEG NLGMANAVLG FLAEKLPVSR WQRDLTDSTV LRNMGVGVGY AVLGFAAHLR GLNKLEPNPA ALAADLDVTW ELLAEPIQTV MRRYGVANPY EKLKDLTRGK GGITPEVLKG FIGSLEIPAE AKAKLLELTP ALYVG
Validated NT Sequence
tgcccacata cagcgcgggg gtcagctcaa gcaatttggc tttggcttcg gcgggaattt ccagcgatcc gataaagcct ttcagcactt cgggtgtgat gccgccttta ccgcgcgtca ggtctttcag tttttcgtaa ggattggcga caccgtaacg gcgcattacg gtttgaatcg gctcggcgag cagctcccaa gtgacatcca aatcggcggc aagcgcggca gggttcggct cgagcttgtt cagaccgcgc aagtgggcgg cgaaacccaa cacggcatag cccacgccca cgcccatatt gcgcaatacg gtgctgtcgg tcaaatcgcg ctgccagcgt gagacgggca gtttttccgc caaaaagccc aatacggcgt ttgccatgcc gaggttgccc tcggagtttt caaagtcgat ggggttgact ttgtgcggca tggtggaaga gccgacttcg cccgctttga ctttttgttt gaaataaccc aatgaaatat aaccccaaac gtcgcggctg aagtcgatga gaatcgtgtt gatgcggctg agggtttgga aaaactccgc catatagtcg tgcggctcga tttggatggt gtaggggttg aaggtcagac cgaggctgat ttcgacgaag ttgcggcagt gtgtttccca atccacatcg ggataggcgg ccatgtgggc gttgtagttg ccgaccgcgc cgttgatttt gccgaggaat tcttgcgcct gaaggatttt gaactggcgt tgcaggcggt acacgacatt ggcggtttct ttgcccaaag tggtcggtgt ggcgggctgg ccgtgggtgc ggctcatcat cgggacggcg gcaaggtcgt gcgccatagc ggtgagtttg ccgatgattt cggcaagttt gggcaggatg acggtttcgc gcgcctcttg cagcatcagg gcgtgggaca ggttgttgat gtcttcgctg gtgcaggcga agtggatgaa ctcgctcacg gcggcgactt ccggcacttc ggcaaaacgt tttttcagcc aatattcgat ggctttgaca tcatgattgg tcgtggcttc gatggctttg acggcggccg cgtcttctaa tgaaaagttt tcgatgactt tgtcgatttc ggcaagcgtt tcggcactga agggcggcac ttcggcaatc tccggctcgg cggccagggc tttgagccag tttaattcga ctttgacgcg cgccttcatc agtccgtact ctgaaaaaat cgggcgcaat gcttcaacgg attgggcata acggccgtct aggggggaaa gcgaggcgat ggggttgatc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMI NPIASLSPLD GRYAQSVEAL RPIFSEYGLM KARVKVELNW LKALAAEPEI AEVPPFSAET LAEIDKVIEN FSLEDAAAVK AIEATTNHDV KAIEYWLKKR FAEVPEVAAV SEFIHFACTS EDINNLSHAL MLQEARETVI LPKLAEIIGK LTAMAHDLAA VPMMSRTHGQ PATPTTLGKE TANVVYRLQR QFKILQAQEF LGKINGAVGN YNAHMAAYPD VDWETHCRNF VEISLGLTFN PYTIQIEPHD YMAEFFQTLS RINTILIDFS RDVWGYISLG YFKQKVKAGE VGSSTMPHKV NPIDFENSEG NLGMANAVLG FLAEKLPVSR WQRDLTDSTV LRNMGVGVGY AVLGFAAHLR GLNKLEPNPA ALAADLDVTW ELLAEPIQTV MRRYGVANPY EKLKDLTHGK DGITPEVLKL FIESLEIPAE AKAKLLELTP ALYVGKAEAL AKRI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca accccatcgc ctcgctttcc cccctagacg gccgttatgc ccaatccgtt gaagcattgc gcccgatttt ttcagagtac ggactgatga aggcgcgcgt caaagtcgaa ttaaactggc tcaaagccct ggccgccgag ccggagattg ccgaagtgcc gcccttcagt gccgaaacgc ttgccgaaat cgacaaagtc atcgaaaact tttcattaga agacgcggcc gccgtcaaag ccatcgaagc cacgaccaat catgatgtca aagccatcga atattggctg aaaaaacgtt ttgccgaagt gccggaagtc gccgccgtga gcgagttcat ccacttcgcc tgcaccagcg aagacatcaa caacctgtcc cacgccctga tgctgcaaga ggcgcgcgaa accgtcatcc tgcccaaact tgccgaaatc atcggcaaac tcaccgctat ggcgcacgac cttgccgccg tcccgatgat gagccgcacc cacggccagc ccgccacacc gaccactttg ggcaaagaaa ccgccaatgt cgtgtaccgc ctgcaacgcc agttcaaaat ccttcaggcg caagaattcc tcggcaaaat caacggcgcg gtcggcaact acaacgccca catggccgcc tatcccgatg tggattggga aacacactgc cgcaacttcg tcgaaatcag cctcggtctg accttcaacc cctacaccat ccaaatcgag ccgcacgact atatggcgga gtttttccaa accctcagcc gcatcaacac gattctcatc gacttcagcc gcgacgtttg gggttatatt tcattgggtt atttcaaaca aaaagtcaaa gcgggcgaag tcggctcttc caccatgccg cacaaagtca accccatcga ctttgaaaac tccgagggca acctcggcat ggcaaacgcc gtattgggct ttttggcgga aaaactgccc gtctcacgct ggcagcgcga tttgaccgac agcaccgtat tgcgcaatat gggcgtgggc gtgggctatg ccgtgttggg tttcgccgcc cacctgcgcg gtctgaacaa gctcgaaccc aaccctgccg cgcttgccgc cgatttggat gtcacttggg agctgctcgc cgagccgatt caaaccgtaa tgcgccgtta cggtgtcgcc aatccttacg agaagctgaa agacctgacg cacggtaaag acggcattac gcccgaagtg ttgaaactct ttatcgaatc gttggaaatt ccggcagaag ccaaagccaa attgcttgag ctgacccctg cgttgtatgt gggcaaggct gaagcgttgg cgaaacggat ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for NegoA.17999.a.B1.PS38036
PURIFICATION DATe: 8/24/2016
CONCENTRATION: 42.25mg/ml
OBSERVED MW: 52kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMI NPIASLSPLD GRYAQSVEAL RPIFSEYGLM KARVKVELNW LKALAAEPEI AEVPPFSAET LAEIDKVIEN FSLEDAAAVK AIEATTNHDV KAIEYWLKKR FAEVPEVAAV SEFIHFACTS EDINNLSHAL MLQEARETVI LPKLAEIIGK LTAMAHDLAA VPMMSRTHGQ PATPTTLGKE TANVVYRLQR QFKILQAQEF LGKINGAVGN YNAHMAAYPD VDWETHCRNF VEISLGLTFN PYTIQIEPHD YMAEFFQTLS RINTILIDFS RDVWGYISLG YFKQKVKAGE VGSSTMPHKV NPIDFENSEG NLGMANAVLG FLAEKLPVSR WQRDLTDSTV LRNMGVGVGY AVLGFAAHLR GLNKLEPNPA ALAADLDVTW ELLAEPIQTV MRRYGVANPY EKLKDLTRGK GGITPEVLKG FIGSLEIPAE AKAKLLELTP ALYVG
Validated NT Sequence
tgcccacata cagcgcgggg gtcagctcaa gcaatttggc tttggcttcg gcgggaattt ccagcgatcc gataaagcct ttcagcactt cgggtgtgat gccgccttta ccgcgcgtca ggtctttcag tttttcgtaa ggattggcga caccgtaacg gcgcattacg gtttgaatcg gctcggcgag cagctcccaa gtgacatcca aatcggcggc aagcgcggca gggttcggct cgagcttgtt cagaccgcgc aagtgggcgg cgaaacccaa cacggcatag cccacgccca cgcccatatt gcgcaatacg gtgctgtcgg tcaaatcgcg ctgccagcgt gagacgggca gtttttccgc caaaaagccc aatacggcgt ttgccatgcc gaggttgccc tcggagtttt caaagtcgat ggggttgact ttgtgcggca tggtggaaga gccgacttcg cccgctttga ctttttgttt gaaataaccc aatgaaatat aaccccaaac gtcgcggctg aagtcgatga gaatcgtgtt gatgcggctg agggtttgga aaaactccgc catatagtcg tgcggctcga tttggatggt gtaggggttg aaggtcagac cgaggctgat ttcgacgaag ttgcggcagt gtgtttccca atccacatcg ggataggcgg ccatgtgggc gttgtagttg ccgaccgcgc cgttgatttt gccgaggaat tcttgcgcct gaaggatttt gaactggcgt tgcaggcggt acacgacatt ggcggtttct ttgcccaaag tggtcggtgt ggcgggctgg ccgtgggtgc ggctcatcat cgggacggcg gcaaggtcgt gcgccatagc ggtgagtttg ccgatgattt cggcaagttt gggcaggatg acggtttcgc gcgcctcttg cagcatcagg gcgtgggaca ggttgttgat gtcttcgctg gtgcaggcga agtggatgaa ctcgctcacg gcggcgactt ccggcacttc ggcaaaacgt tttttcagcc aatattcgat ggctttgaca tcatgattgg tcgtggcttc gatggctttg acggcggccg cgtcttctaa tgaaaagttt tcgatgactt tgtcgatttc ggcaagcgtt tcggcactga agggcggcac ttcggcaatc tccggctcgg cggccagggc tttgagccag tttaattcga ctttgacgcg cgccttcatc agtccgtact ctgaaaaaat cgggcgcaat gcttcaacgg attgggcata acggccgtct aggggggaaa gcgaggcgat ggggttgatc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMI NPIASLSPLD GRYAQSVEAL RPIFSEYGLM KARVKVELNW LKALAAEPEI AEVPPFSAET LAEIDKVIEN FSLEDAAAVK AIEATTNHDV KAIEYWLKKR FAEVPEVAAV SEFIHFACTS EDINNLSHAL MLQEARETVI LPKLAEIIGK LTAMAHDLAA VPMMSRTHGQ PATPTTLGKE TANVVYRLQR QFKILQAQEF LGKINGAVGN YNAHMAAYPD VDWETHCRNF VEISLGLTFN PYTIQIEPHD YMAEFFQTLS RINTILIDFS RDVWGYISLG YFKQKVKAGE VGSSTMPHKV NPIDFENSEG NLGMANAVLG FLAEKLPVSR WQRDLTDSTV LRNMGVGVGY AVLGFAAHLR GLNKLEPNPA ALAADLDVTW ELLAEPIQTV MRRYGVANPY EKLKDLTHGK DGITPEVLKL FIESLEIPAE AKAKLLELTP ALYVGKAEAL AKRI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCA ACCCCATCGC CTCGCTTTCC CCCCTAGACG GCCGTTATGC CCAATCCGTT GAAGCATTGC GCCCGATTTT TTCAGAGTAC GGACTGATGA AGGCGCGCGT CAAAGTCGAA TTAAACTGGC TCAAAGCCCT GGCCGCCGAG CCGGAGATTG CCGAAGTGCC GCCCTTCAGT GCCGAAACGC TTGCCGAAAT CGACAAAGTC ATCGAAAACT TTTCATTAGA AGACGCGGCC GCCGTCAAAG CCATCGAAGC CACGACCAAT CATGATGTCA AAGCCATCGA ATATTGGCTG AAAAAACGTT TTGCCGAAGT GCCGGAAGTC GCCGCCGTGA GCGAGTTCAT CCACTTCGCC TGCACCAGCG AAGACATCAA CAACCTGTCC CACGCCCTGA TGCTGCAAGA GGCGCGCGAA ACCGTCATCC TGCCCAAACT TGCCGAAATC ATCGGCAAAC TCACCGCTAT GGCGCACGAC CTTGCCGCCG TCCCGATGAT GAGCCGCACC CACGGCCAGC CCGCCACACC GACCACTTTG GGCAAAGAAA CCGCCAATGT CGTGTACCGC CTGCAACGCC AGTTCAAAAT CCTTCAGGCG CAAGAATTCC TCGGCAAAAT CAACGGCGCG GTCGGCAACT ACAACGCCCA CATGGCCGCC TATCCCGATG TGGATTGGGA AACACACTGC CGCAACTTCG TCGAAATCAG CCTCGGTCTG ACCTTCAACC CCTACACCAT CCAAATCGAG CCGCACGACT ATATGGCGGA GTTTTTCCAA ACCCTCAGCC GCATCAACAC GATTCTCATC GACTTCAGCC GCGACGTTTG GGGTTATATT TCATTGGGTT ATTTCAAACA AAAAGTCAAA GCGGGCGAAG TCGGCTCTTC CACCATGCCG CACAAAGTCA ACCCCATCGA CTTTGAAAAC TCCGAGGGCA ACCTCGGCAT GGCAAACGCC GTATTGGGCT TTTTGGCGGA AAAACTGCCC GTCTCACGCT GGCAGCGCGA TTTGACCGAC AGCACCGTAT TGCGCAATAT GGGCGTGGGC GTGGGCTATG CCGTGTTGGG TTTCGCCGCC CACCTGCGCG GTCTGAACAA GCTCGAACCC AACCCTGCCG CGCTTGCCGC CGATTTGGAT GTCACTTGGG AGCTGCTCGC CGAGCCGATT CAAACCGTAA TGCGCCGTTA CGGTGTCGCC AATCCTTACG AGAAGCTGAA AGACCTGACG CACGGTAAAG ACGGCATTAC GCCCGAAGTG TTGAAACTCT TTATCGAATC GTTGGAAATT CCGGCAGAAG CCAAAGCCAA ATTGCTTGAG CTGACCCCTG CGTTGTATGT GGGCAAGGCT GAAGCGTTGG CGAAACGGAT TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT