NegoA.17993.a

glycyl-tRNA synthetase alpha subunit

CENTER ID:	NegoA.17993.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17993.a.B1.GE40242	full length		1	298

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17993.a.B1.PS38039	full length		1	298

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_85050

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.2846
UniProt:	B4RJG2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLTFQQIIFK LQTFWADKGC TVIQPFDMEV GAGTSHPATC LRALGPEPWF AAYVQPSRRP KDGRYGDNPN RLQHYYQFQI ALKPAPANIQ DLYLDSLREL GIDPKVHDIR FVEDDWENPT LGAWGLGWEV WLNGMEVTQF TYFQQVGGID CTPVLGEITY GIERLAMYLQ GVENVYDLVW AKTPDGNTVS YGDVYHQNEV EQSTYNFEYS DADWLLRQFN DYEAQAKRLF AEENADLALP AYELVLKAGH TFNLLDARGA ISVTERATYI GRIRALSRAV AQKYIESREK LGFPLIKK

NT Sequence

ATGCTTACCT TCCAACAAAT CATCTTCAAA CTGCAAACCT TCTGGGCAGA CAAAGGCTGC ACCGTCATCC AACCCTTCGA CATGGAAGTC GGTGCCGGCA CATCCCATCC CGCCACCTGC CTGCGCGCGC TCGGCCCCGA GCCTTGGTTT GCCGCCTACG TCCAACCCAG CCGCCGCCCC AAAGACGGCC GCTACGGCGA CAACCCCAAC CGCCTGCAAC ACTATTACCA ATTCCAAATC GCCCTCAAAC CCGCCCCCGC CAATATCCAA GACCTCTATC TCGACTCCCT GCGCGAATTG GGCATCGATC CCAAAGTCCA CGACATCCGC TTTGTCGAAG ACGACTGGGA AAACCCCACC CTCGGTGCGT GGGGTTTGGG CTGGGAAGTC TGGCTCAACG GCATGGAAGT AACCCAGTTT ACCTATTTCC AACAAGTCGG CGGTATCGAT TGCACGCCCG TACTCGGCGA AATTACCTAC GGCATCGAAC GCTTGGCGAT GTACCTGCAA GGCGTGGAAA ACGTCTATGA CCTCGTTTGG GCAAAAACGC CGGACGGCAA TACCGTAAGC TACGGCGACG TGTACCATCA AAACGAAGTC GAGCAATCTA CCTACAACTT CGAATACAGC GATGCCGACT GGCTGCTGCG CCAGTTCAAC GACTACGAAG CGCAAGCCAA ACGCCTGTTC GCCGAAGAAA ACGCCGACCT TGCCTTACCT GCCTACGAGC TGGTCCTCAA AGCGGGGCAT ACGTTCAACC TTTTAGACGC ACGCGGCGCG ATTTCCGTAA CCGAGCGGGC AACTTATATT GGACGTATTC GTGCATTGAG CCGCGCCGTG GCGCAAAAAT ATATCGAAAG CCGTGAGAAA CTGGGCTTCC CGTTGATTAA AAAATAA

Details for NegoA.17993.a.B1.GE40242

HARVESTED ON:	5/9/2016
SEQUENCED ON:	5/23/2016
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML TFQQIIFKLQ TFWADKGCTV IQPFDMEVGA GTSHPATCLR ALGPEPWFAA YVQPSRRPKD GRYGDNPNRL QHYYQFQVAL KPAPANIQDL YLDSLRELGI DPKVHDIRFV EDDWENPTLG AWGLGWEVWL NGMEVTQFTY FQQVGGIDCT PVLGEITYGI ERLAMYLQGV ENVYDLVWAK TPDGNTVSYG DVYHQNEVEQ STYNFEYSDA DWLLRQFNDY EAQAKRLFAE ENAGLALPAY ELVLKAGHTF NLLDARGAIS VTERATYIGR IRALSRAVAQ KYIESREKXG FPLIKK

Validated NT Sequence

ttactcactt attttttaat caacgggaag ccnnntttct cacggctttc gatatatttt tgcgccacgg cgcggctcaa tgcacgaata cgtccaatat aagttgcccg ctcggttacg gaaatcgcgc cgcgtgcgtc taaaaggttg aacgtatgcc ccgctttgag gaccagctcg taggcaggta aggcaaggcc ggcgttttct tcggcgaaca ggcgtttggc ttgcgcttcg tagtcgttga actggcgcag cagccagtcg gcatcgctgt attcgaagtt gtaggtagat tgctcgactt cgttttgatg gtacacgtcg ccgtagctta cggtattgcc gtccggcgtt tttgcccaaa cgaggtcata gacgttttcc acgccttgca ggtacatcgc caagcgttcg atgccgtagg taatttcgcc gagtacgggc gtgcaatcga taccgccgac ttgttggaaa taggtaaact gggttacttc catgccgttg agccagactt cccagcccaa accccacgca ccgagggtgg ggttttccca gtcgtcttcg acaaagcgga tgtcgtggac tttgggatcg atgcccaatt cgcgcaggga gtcgagatag aggtcttgga tattggcggg ggcgggtttg agggcgactt ggaattggta atagtgttgc aggcggttgg ggttgtcgcc gtagcggccg tctttggggc ggcggctggg ttggacgtag gcggcaaacc aaggctcggg gccgagcgcg cgcaggcagg tggcgggatg ggatgtgccg gcaccgactt ccatgtcgaa gggttggatg acggtgcagc ctttgtctgc ccagaaggtt tgcagtttga agatgatttg ttggaaggta agcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHML TFQQIIFKLQ TFWADKGCTV IQPFDMEVGA GTSHPATCLR ALGPEPWFAA YVQPSRRPKD GRYGDNPNRL QHYYQFQIAL KPAPANIQDL YLDSLRELGI DPKVHDIRFV EDDWENPTLG AWGLGWEVWL NGMEVTQFTY FQQVGGIDCT PVLGEITYGI ERLAMYLQGV ENVYDLVWAK TPDGNTVSYG DVYHQNEVEQ STYNFEYSDA DWLLRQFNDY EAQAKRLFAE ENADLALPAY ELVLKAGHTF NLLDARGAIS VTERATYIGR IRALSRAVAQ KYIESREKLG FPLIKK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctta ccttccaaca aatcatcttc aaactgcaaa ccttctgggc agacaaaggc tgcaccgtca tccaaccctt cgacatggaa gtcggtgccg gcacatccca tcccgccacc tgcctgcgcg cgctcggccc cgagccttgg tttgccgcct acgtccaacc cagccgccgc cccaaagacg gccgctacgg cgacaacccc aaccgcctgc aacactatta ccaattccaa atcgccctca aacccgcccc cgccaatatc caagacctct atctcgactc cctgcgcgaa ttgggcatcg atcccaaagt ccacgacatc cgctttgtcg aagacgactg ggaaaacccc accctcggtg cgtggggttt gggctgggaa gtctggctca acggcatgga agtaacccag tttacctatt tccaacaagt cggcggtatc gattgcacgc ccgtactcgg cgaaattacc tacggcatcg aacgcttggc gatgtacctg caaggcgtgg aaaacgtcta tgacctcgtt tgggcaaaaa cgccggacgg caataccgta agctacggcg acgtgtacca tcaaaacgaa gtcgagcaat ctacctacaa cttcgaatac agcgatgccg actggctgct gcgccagttc aacgactacg aagcgcaagc caaacgcctg ttcgccgaag aaaacgccga ccttgcctta cctgcctacg agctggtcct caaagcgggg catacgttca accttttaga cgcacgcggc gcgatttccg taaccgagcg ggcaacttat attggacgta ttcgtgcatt gagccgcgcc gtggcgcaaa aatatatcga aagccgtgag aaactgggct tcccgttgat taaaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for NegoA.17993.a.B1.PS38039

PURIFICATION DATe:	8/30/2016
CONCENTRATION:	27mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTTA CCTTCCAACA AATCATCTTC AAACTGCAAA CCTTCTGGGC AGACAAAGGC TGCACCGTCA TCCAACCCTT CGACATGGAA GTCGGTGCCG GCACATCCCA TCCCGCCACC TGCCTGCGCG CGCTCGGCCC CGAGCCTTGG TTTGCCGCCT ACGTCCAACC CAGCCGCCGC CCCAAAGACG GCCGCTACGG CGACAACCCC AACCGCCTGC AACACTATTA CCAATTCCAA ATCGCCCTCA AACCCGCCCC CGCCAATATC CAAGACCTCT ATCTCGACTC CCTGCGCGAA TTGGGCATCG ATCCCAAAGT CCACGACATC CGCTTTGTCG AAGACGACTG GGAAAACCCC ACCCTCGGTG CGTGGGGTTT GGGCTGGGAA GTCTGGCTCA ACGGCATGGA AGTAACCCAG TTTACCTATT TCCAACAAGT CGGCGGTATC GATTGCACGC CCGTACTCGG CGAAATTACC TACGGCATCG AACGCTTGGC GATGTACCTG CAAGGCGTGG AAAACGTCTA TGACCTCGTT TGGGCAAAAA CGCCGGACGG CAATACCGTA AGCTACGGCG ACGTGTACCA TCAAAACGAA GTCGAGCAAT CTACCTACAA CTTCGAATAC AGCGATGCCG ACTGGCTGCT GCGCCAGTTC AACGACTACG AAGCGCAAGC CAAACGCCTG TTCGCCGAAG AAAACGCCGA CCTTGCCTTA CCTGCCTACG AGCTGGTCCT CAAAGCGGGG CATACGTTCA ACCTTTTAGA CGCACGCGGC GCGATTTCCG TAACCGAGCG GGCAACTTAT ATTGGACGTA TTCGTGCATT GAGCCGCGCC GTGGCGCAAA AATATATCGA AAGCCGTGAG AAACTGGGCT TCCCGTTGAT TAAAAAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT