NegoA.17969.a

tRNA threonylcarbamoyladenosine biosynthesis protein RimN

CENTER ID: NegoA.17969.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17969.a.B2.GE40241 mature protein 20 200
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.2484
UniProt: B4RPN8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDALQTAFY IMLFSRIIAA SAQRKLSVYL KKGGLVAYPT ESCYGLGCLP TLAKALGKLA HLKKRPQHKG MIVIGNQLEQ LQPLLQMPFE NIQTMLRNEW PAPKTFLLLA KSGVLPALRG KRRSKLAVRV PDHTGARRLC QALGMPLVST SCNRAGKRAC RTEREVRRQF GRDVWIVGGR IGRQKSPSQI IDGETGKRLR
NT Sequence
ATGACTGATG CGCTTCAGAC GGCATTTTAT ATTATGTTAT TTTCGAGAAT TATCGCAGCA TCAGCGCAAC GTAAGTTAAG CGTTTATTTG AAAAAAGGCG GTTTGGTTGC TTATCCGACC GAATCTTGTT ATGGCTTGGG GTGTCTTCCG ACATTAGCCA AAGCACTCGG TAAGTTGGCG CATTTGAAAA AAAGGCCGCA ACACAAGGGT ATGATTGTCA TCGGGAATCA ACTTGAACAA TTGCAGCCTT TACTTCAAAT GCCGTTTGAA AACATTCAAA CCATGCTTAG AAACGAATGG CCTGCTCCTA AGACTTTCCT ATTGTTGGCT AAATCTGGCG TATTACCTGC GTTGCGCGGG AAGAGGCGCA GCAAGCTTGC GGTCAGAGTG CCCGATCATA CAGGTGCAAG GCGTTTGTGT CAGGCATTGG GTATGCCTTT GGTTTCCACT TCATGTAATC GGGCGGGTAA ACGCGCGTGT CGGACGGAGA GGGAAGTCAG AAGGCAGTTC GGACGCGATG TGTGGATTGT CGGTGGGAGA ATCGGCAGGC AGAAATCGCC GAGTCAGATT ATCGATGGGG AAACAGGTAA ACGTTTGCGT TAG
Details for NegoA.17969.a.B2.GE40241
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/24/2016
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA SAQRKLSVYL KKGGLVAYPT ESCYGLGCLP TLAKALGKLA HLKKRPQHKG MIVIGNQLEQ LQPLLQMPSE NIQTMLRNEW PAPKTFLLLA KSGVLPALRG KRRSKLAVRV PDHTGARRLC QALGMPLVST SCNRAGKRAC RTEREVRRQF GRDVWIVGGR IGRQKSPSQI IDGETGKRLR
Validated NT Sequence
atcttactca cttaacgcaa acgtttacct gtttccccat cgataatctg actcggcgat ttctgcctgc cgattctccc accgacaatc cacacatcgc gtccgaactg ccttctgact tccctctccg tccgacacgc gcgtttaccc gcccgattac atgaagtgga aaccaaaggc atacccaatg cctgacacaa acgccttgca cctgtatgat cgggcactct gaccgcaagc ttgctgcgcc tcttcccgcg caacgcaggt aatacgccag atttagccaa caataggaaa gtcttaggag caggccattc gtttctaagc atggtttgaa tgttttcaga cggcatttga agtaaaggct gcaattgttc aagttgattc ccgatgacaa tcataccctt gtgttgcggc ctttttttca aatgcgccaa cttaccgagt gctttggcta atgtcggaag acaccccaag ccataacaag attcggtcgg ataagcaacc aaaccgcctt ttttcaaata aacgcttaac ttacgttgcg ctgatgccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHAS AQRKLSVYLK KGGLVAYPTE SCYGLGCLPT LAKALGKLAH LKKRPQHKGM IVIGNQLEQL QPLLQMPFEN IQTMLRNEWP APKTFLLLAK SGVLPALRGK RRSKLAVRVP DHTGARRLCQ ALGMPLVSTS CNRAGKRACR TEREVRRQFG RDVWIVGGRI GRQKSPSQII DGETGKRLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcatcag cgcaacgtaa gttaagcgtt tatttgaaaa aaggcggttt ggttgcttat ccgaccgaat cttgttatgg cttggggtgt cttccgacat tagccaaagc actcggtaag ttggcgcatt tgaaaaaaag gccgcaacac aagggtatga ttgtcatcgg gaatcaactt gaacaattgc agcctttact tcaaatgccg tttgaaaaca ttcaaaccat gcttagaaac gaatggcctg ctcctaagac tttcctattg ttggctaaat ctggcgtatt acctgcgttg cgcgggaaga ggcgcagcaa gcttgcggtc agagtgcccg atcatacagg tgcaaggcgt ttgtgtcagg cattgggtat gcctttggtt tccacttcat gtaatcgggc gggtaaacgc gcgtgtcgga cggagaggga agtcagaagg cagttcggac gcgatgtgtg gattgtcggt gggagaatcg gcaggcagaa atcgccgagt cagattatcg atggggaaac aggtaaacgt ttgcgttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t