NegoA.17968.a

major outer membrane protein P.IB

CENTER ID: NegoA.17968.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17968.a.B2.GE40324 mature protein 19 350
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKSLIALTL AALPVAATAD VTLYGAIKAG VQTYRSVEHT DGKVSKVETG SEIADFGSKI GFKGQEDLGN GLKAVWQLEQ GASVAGTNTG WGNKQSFVGL KGGFGTIRAG SLNSPLKNTD DNVNAWESGK FTGNVLEISG MAKREHRYLS VRYDSPEFAG FSGSVQYAPK DNSGSNGESY HVGLNYQNSG FFAQYAGLFQ RYGEGTKKIE YEHQVYSIPS LFVEKLQVHR LVGGYDNNAL YVSVAAQQQD AKLYQNQLVR DNSHNSQTEV AATVAYRFGN VTPRVSYAHG FKGTVDSADH DNTYDQVVVG AEYDFSKRTS ALVSAGWLQE GKGADKIVST ASAVVLRHKF
NT Sequence
ATGAAAAAAT CCCTGATTGC CCTGACTTTG GCAGCCCTTC CTGTTGCGGC AACGGCCGAT GTCACCCTGT ACGGTGCCAT CAAAGCCGGC GTACAAACTT ACCGTTCTGT AGAACATACA GACGGCAAGG TAAGTAAAGT GGAAACCGGC AGCGAAATCG CCGACTTCGG TTCAAAAATC GGCTTCAAAG GCCAAGAAGA CCTCGGCAAC GGTCTGAAGG CCGTTTGGCA GTTGGAACAA GGTGCCTCCG TCGCCGGCAC TAACACCGGC TGGGGCAACA AACAATCCTT CGTCGGCTTG AAGGGCGGCT TCGGTACCAT CCGCGCCGGT AGCCTGAACA GCCCCCTGAA AAACACCGAC GACAACGTCA ATGCTTGGGA ATCCGGCAAA TTTACCGGCA ATGTGCTGGA AATCAGCGGA ATGGCCAAAC GGGAACACCG CTACCTGTCC GTACGCTACG ATTCTCCCGA ATTTGCCGGC TTCAGCGGCA GCGTACAATA CGCACCTAAA GACAATTCAG GCTCAAACGG CGAATCTTAC CACGTTGGCT TGAACTACCA AAACAGCGGC TTCTTCGCAC AATACGCCGG CTTGTTCCAA AGATACGGCG AAGGCACTAA AAAAATCGAA TACGAACATC AAGTTTATAG TATCCCCAGC CTGTTTGTTG AAAAACTGCA AGTTCACCGT TTGGTAGGCG GTTACGACAA TAATGCCCTG TACGTCTCCG TAGCCGCACA ACAACAAGAT GCCAAATTGT ATCAAAATCA ATTAGTGCGT GATAATTCGC ACAACTCTCA AACCGAAGTT GCCGCTACCG TGGCATACCG TTTCGGCAAC GTAACGCCCC GCGTTTCTTA CGCCCACGGC TTCAAAGGCA CTGTTGATAG TGCAGACCAC GACAATACTT ATGACCAAGT GGTTGTCGGC GCGGAATACG ACTTCTCCAA ACGCACTTCT GCCTTGGTTT CTGCCGGCTG GTTGCAAGAA GGCAAAGGCG CAGACAAAAT CGTATCGACT GCCAGCGCCG TCGTTCTGCG CCACAAATTC TAA
Details for NegoA.17968.a.B2.GE40324
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/17/2016
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMA DVTLYGAIKA GVQTYRSVEH TDGKVSKVET GSEIADFGSK IGFKGQEDLG NGLKAVWQLE QGASVAGTNT GWGNKQSFVG LKGGFGTIRA GSLNSPLKNT GANVNAWESG KFTGNVLEIS GMAQREHRYL SVRYDSPEFA GFSGSVQYAP KDNSGSNGES YHVGLNYQNS GFFAQYAGLF QRYGEGTKKI EYDGQTYSIP SLFVEKLQVH RLVGGYDNNA LYVSVAAQQQ DAKLYGAMSG NSHNSQTEVA ATAAYRFGNV TPRVSYAHGF KGTVDSANHD NTYDQVVVGA EYDFSKRTSA LVSAGWLQEG KGADKIVSTA SXXXXRHKF
Validated NT Sequence
atcttactca cttagaattt gtggcgcann nnnnnnnngc tggcagtcga tacgattttg tctgcgcctt tgccttcttg caaccagccg gcagaaacca aggcagaagt gcgtttggag aagtcgtatt ccgcaccgac aaccacttgg tcataagtat tgtcgtggtt tgcactatca acagtgcctt tgaagccgtg ggcgtaagaa acgcggggcg ttacattgcc gaaacggtat gccgcggtag cggcaacttc ggtttgagag ttgtgcgaat taccgctcat tgctccatac aatttggcat cttgttgttg tgcggctacg gaaacgtaca gggcattatt gtcgtaaccg cctaccaaac ggtgaacttg cagtttttca acaaacagac tggggatact ataagtttga ccatcgtatt cgattttttt agtgccttcg ccgtatcttt ggaacaagcc ggcgtattgc gcgaagaagc cgctgttttg gtagttcaag ccaacgtggt aagattcgcc gtttgagcct gaattgtctt taggtgcgta ttgtacgctg ccgctgaagc cggcaaattc gggagaatcg tagcgtacgg acaggtagcg gtgttcccgt tgggccattc cgctgatttc cagcacattg ccggtaaatt tgccggattc ccaagcattg acgttggcgc cggtgttttt cagggggctg ttcaggctac cggcgcggat ggtaccgaag ccgcccttca agccgacgaa ggattgtttg ttgccccagc cggtgttagt gccggcgacg gaggcacctt gttccaactg ccaaacggcc ttcaggccgt tgccgaggtc ttcttggcct ttgaagccga tttttgaacc gaagtcggcg atttcgctgc cggtttccac tttacttacc ttgccgtctg tatgttctac agaacggtaa gtttgtacgc cggctttgat ggcgccgtaa agggtgacat cggccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHAD VTLYGAIKAG VQTYRSVEHT DGKVSKVETG SEIADFGSKI GFKGQEDLGN GLKAVWQLEQ GASVAGTNTG WGNKQSFVGL KGGFGTIRAG SLNSPLKNTD DNVNAWESGK FTGNVLEISG MAKREHRYLS VRYDSPEFAG FSGSVQYAPK DNSGSNGESY HVGLNYQNSG FFAQYAGLFQ RYGEGTKKIE YEHQVYSIPS LFVEKLQVHR LVGGYDNNAL YVSVAAQQQD AKLYQNQLVR DNSHNSQTEV AATVAYRFGN VTPRVSYAHG FKGTVDSADH DNTYDQVVVG AEYDFSKRTS ALVSAGWLQE GKGADKIVST ASAVVLRHKF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgatg tcaccctgta cggtgccatc aaagccggcg tacaaactta ccgttctgta gaacatacag acggcaaggt aagtaaagtg gaaaccggca gcgaaatcgc cgacttcggt tcaaaaatcg gcttcaaagg ccaagaagac ctcggcaacg gtctgaaggc cgtttggcag ttggaacaag gtgcctccgt cgccggcact aacaccggct ggggcaacaa acaatccttc gtcggcttga agggcggctt cggtaccatc cgcgccggta gcctgaacag ccccctgaaa aacaccgacg acaacgtcaa tgcttgggaa tccggcaaat ttaccggcaa tgtgctggaa atcagcggaa tggccaaacg ggaacaccgc tacctgtccg tacgctacga ttctcccgaa tttgccggct tcagcggcag cgtacaatac gcacctaaag acaattcagg ctcaaacggc gaatcttacc acgttggctt gaactaccaa aacagcggct tcttcgcaca atacgccggc ttgttccaaa gatacggcga aggcactaaa aaaatcgaat acgaacatca agtttatagt atccccagcc tgtttgttga aaaactgcaa gttcaccgtt tggtaggcgg ttacgacaat aatgccctgt acgtctccgt agccgcacaa caacaagatg ccaaattgta tcaaaatcaa ttagtgcgtg ataattcgca caactctcaa accgaagttg ccgctaccgt ggcataccgt ttcggcaacg taacgccccg cgtttcttac gcccacggct tcaaaggcac tgttgatagt gcagaccacg acaatactta tgaccaagtg gttgtcggcg cggaatacga cttctccaaa cgcacttctg ccttggtttc tgccggctgg ttgcaagaag gcaaaggcgc agacaaaatc gtatcgactg ccagcgccgt cgttctgcgc cacaaattct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat