NegoA.17953.a

electron transfer flavoprotein beta subunit ETFB

CENTER ID: NegoA.17953.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17953.a.B1.GE40083 full length 1 249
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.2489
UniProt: B4RPP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKALVAVKRV VDYNVKVRVK ADGSDVDIGN VKMSMNPFDE IAVEEAVRLK EAGKVSEIVA VSLGEKKCEE TLRTALAMGA DRAIHIETDV KLEPLAVAKL LKAVADKENP QIFFLGKQAI DDDANQVAQM LAALLNAAQG TFVSKVQIEG DEVQIVREID GGEETIALKL PAVISADLRL NEPRFVKLPN IMAAKKKPLE KLDSADLATD ISPRLKTVKF AEPKARQAGV KVASVAELVE KLKNEAKVI
NT Sequence
ATGAAAGCAC TGGTCGCAGT AAAGCGCGTA GTGGACTACA ACGTCAAAGT CCGTGTGAAA GCCGATGGTT CGGATGTGGA CATCGGCAAT GTCAAAATGT CGATGAACCC GTTTGATGAA ATCGCTGTGG AAGAAGCCGT CCGTTTGAAA GAAGCCGGGA AAGTAAGCGA AATTGTAGCG GTTTCTTTGG GTGAAAAGAA ATGCGAAGAA ACCTTGCGTA CCGCTTTGGC AATGGGTGCC GACCGTGCCA TTCATATTGA AACCGATGTG AAATTAGAGC CGCTGGCAGT TGCCAAACTG CTGAAAGCTG TTGCGGACAA AGAAAATCCG CAAATTTTCT TTTTGGGCAA ACAAGCGATT GATGACGATG CCAATCAAGT GGCGCAAATG CTGGCAGCTT TGTTGAATGC GGCGCAAGGT ACATTTGTTT CAAAAGTACA AATTGAAGGT GATGAAGTAC AGATTGTGCG CGAAATTGAT GGCGGCGAAG AAACGATTGC GTTGAAACTG CCTGCAGTCA TCAGCGCAGA TTTGCGTTTG AACGAGCCGC GCTTTGTCAA ACTCCCCAAT ATTATGGCGG CAAAGAAAAA ACCTCTGGAA AAACTGGATT CTGCCGATTT GGCTACCGAT ATTTCGCCTC GTCTGAAAAC GGTGAAATTT GCCGAACCTA AGGCGCGTCA GGCAGGCGTA AAAGTAGCAA GCGTTGCCGA ATTGGTTGAA AAATTGAAAA ACGAAGCCAA AGTGATTTGA
Details for NegoA.17953.a.B1.GE40083
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK ALVAVKRVVD YNVKVRVKAD GSDVDIGNVK MSMNPFDEIA VEEAVRLKEA GKVSEIVAVS LGEKKCEETL RTALAMGADR AIHIETDVKL EPLAVAKLLK AVADKENPQI FFLGKQAIDD DANQVAQMLA ALLNAAQGTF VSKVQIEGDE VQIVREIDGG EETIALKLPA VISADLRLNE PRFVKLPNIM VAKKKPLEKL DSADLATDIS PRLKTVKFAE PKARQAGVKV ASVAELVEKL KNEAKVI
Validated NT Sequence
atcttactca cttaaatcac tttggcttcg tttttcaatt tttcaaccaa ttcggcaacg cttgctactt ttacgcctgc ctgacgcgcc ttaggttcgg caaatttcac cgttttcaga cgaggcgaaa tatcggtagc caaatcggca gaatccagtt tttccagagg ttttttcttt gccaccataa tattggggag tttgacaaag cgcggctcgt tcaaacgcaa atctgcgctg atgactgcag gcagtttcaa cgcaatcgtt tcttcgccgc catcaatttc gcgcacaatc tgtacttcat caccttcaat ttgtactttt gaaacaaatg taccttgcgc cgcattcagc aaagctgcca gcatttgcgc cacttgattg gcatcgtcat caatcgcttg tttgcccaaa aagaaaattt gcggattttc tttgtccgca acagctttca gcagtttggc aactgccagc ggctctaatt tcacatcggt ttcaatatga atggcacggt cggcacccat tgccaaagcg gtacgcaagg tttcttcgca tttcttttca cccaaagaaa ccgctacaat ttcgcttact tttccggctt ctttcaaacg gacggcttct tccacagcga tttcatcaaa cgggttcatc gacattttga cattgccgat gtccacatcc gaaccatcgg ctttcacacg gactttgacg ttgtagtcca ctacgcgctt tactgcgacc agtgctttca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMK ALVAVKRVVD YNVKVRVKAD GSDVDIGNVK MSMNPFDEIA VEEAVRLKEA GKVSEIVAVS LGEKKCEETL RTALAMGADR AIHIETDVKL EPLAVAKLLK AVADKENPQI FFLGKQAIDD DANQVAQMLA ALLNAAQGTF VSKVQIEGDE VQIVREIDGG EETIALKLPA VISADLRLNE PRFVKLPNIM AAKKKPLEKL DSADLATDIS PRLKTVKFAE PKARQAGVKV ASVAELVEKL KNEAKVI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag cactggtcgc agtaaagcgc gtagtggact acaacgtcaa agtccgtgtg aaagccgatg gttcggatgt ggacatcggc aatgtcaaaa tgtcgatgaa cccgtttgat gaaatcgctg tggaagaagc cgtccgtttg aaagaagccg ggaaagtaag cgaaattgta gcggtttctt tgggtgaaaa gaaatgcgaa gaaaccttgc gtaccgcttt ggcaatgggt gccgaccgtg ccattcatat tgaaaccgat gtgaaattag agccgctggc agttgccaaa ctgctgaaag ctgttgcgga caaagaaaat ccgcaaattt tctttttggg caaacaagcg attgatgacg atgccaatca agtggcgcaa atgctggcag ctttgttgaa tgcggcgcaa ggtacatttg tttcaaaagt acaaattgaa ggtgatgaag tacagattgt gcgcgaaatt gatggcggcg aagaaacgat tgcgttgaaa ctgcctgcag tcatcagcgc agatttgcgt ttgaacgagc cgcgctttgt caaactcccc aatattatgg cggcaaagaa aaaacctctg gaaaaactgg attctgccga tttggctacc gatatttcgc ctcgtctgaa aacggtgaaa tttgccgaac ctaaggcgcg tcaggcaggc gtaaaagtag caagcgttgc cgaattggtt gaaaaattga aaaacgaagc caaagtgatt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat