NegoA.17946.a

DNA ligase

CENTER ID: NegoA.17946.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
NegoA.17946.a.B1.GE39725 full length 1 823
NegoA.17946.a.B2.GE40396 Domain 2 1 535
NegoA.17946.a.B4.GE40398 Domain 4 1 383
NegoA.17946.a.B6.GE40399 Domain 6 161 535
NegoA.17946.a.B7.GE40400 Domain 7 161 823
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
NegoA.17946.a.B4.PS38107 Domain 4 1 383
NegoA.17946.a.B1.PW37842 full length 1 823
Molecular models
METHOD RESULTS
comparative modelling Robetta_85048
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|521006.8.peg.412
UniProt: B4RJQ9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPTAQRIHE LTDLLNRYAY EYYTLDAPSI PDAEYDRLFR ELEALERNHP ELKLPDSPTQ RVGGEPLAGF AEVRHEVPML SLTNAFSPQD ENGVFDHAEM YAFDQRVRDG LDGGNPEYVI EPKFDGLAIS LLYRDGVLVQ AATRGDGTTG EDVTRNVKTV SNIPLRLHGE NVPELIEVRG EVLMLKADFA GLNKRQAENG QKPFANPRNA AAGSLRQLDS RITAQRKLHF FPYSIARQQG GFEAEEHIQE LAYFQELGFS LPNGNFGCFK NIGEVLAFYE HMQQKRPELP YEIDGMVVKV NSLAQQRELG FISRAPRWAV AHKFPAEEAL TIVEAIDVQI GRTGAVTPVA RLQPVFVGGV TVTNATLHNQ DEVSRKDVRV GDTVVVRRAG DVIPEVVRVI FERRPMQETA VAVSDGIGHQ QDDLFAETPS AKQTESVPLH KPYRLPARCP ICRSEIEREE GEAVARCSGG MLCQAQRAQG LIHFASRKAM DIDGLGEKQI EQLVAQDLVR HFADLYRIDI PTLQKMKETA DKGSSENENG DAETVSGDLS KYNTQNGKKQ PTKWAQNILA GIESGKTPEL ARFLFALGIR HVGERTAKTL AQAFGTLERV RRAPEPVLAC LPDIGTVVAR SIAHFFAQAE QQAMIDELLA AGVAPQAQAV SLPAAQYAGP QRWITRLPGF KISENKAQAL WELAGQSIEG LQNDKALPAD WQAWRSKAQN TALLENLKTF FAQMPSEDEA AQGSDGINKA VAGKTFVLTG TLPTFKRDQA QALIEAAGGK VSGSVSKKTD YVVAGEAAGS KLEKANALGV SVLSEAELLT LLC
NT Sequence
atgaacccga ccgcacaacg catacacgaa ctcaccgacc tcctcaaccg ctacgcctac gaatactaca ccctcgacgc gcccagcata cccgatgccg aatacgaccg cctgttccgc gaactcgaag cgttggaacg aaaccatccc gagctcaagc tgcccgacag cccgacccag cgcgtcggcg gcgagccttt ggcaggcttt gccgaagtgc gccacgaagt gccgatgctg tcgctgacca acgccttttc cccgcaagat gaaaacggcg tgttcgacca tgccgaaatg tacgccttcg accaacgcgt gcgcgacggc ttggacggcg gcaatcccga atacgttatc gaacccaaat tcgacggcct cgccatcagc ctgctctacc gcgacggcgt attggtgcag gcggcaacgc gcggcgacgg cacgacgggc gaagacgtta cccgaaacgt caaaaccgta tccaacatcc ccctgcggct gcacggcgaa aacgtgcccg aactcatcga ggtacgcggc gaagtgctga tgctcaaagc cgactttgcc gggctcaata aacgccaagc cgaaaacggg caaaaaccct ttgccaatcc gcgcaacgcc gccgccggca gcctgcgcca actcgattcg cgcatcaccg cgcaacgcaa actgcacttt ttcccctatt cgattgcccg tcagcaaggc ggtttcgaag ctgaagaaca catccaagaa ctcgcctatt tccaagaact cggcttcagt ctgcccaatg gcaatttcgg ctgtttcaaa aatatcggcg aagtattggc gttttacgaa cacatgcagc aaaaacgccc cgaactgccc tacgaaatcg acggcatggt ggtcaaagtc aacagcttgg cgcaacagcg cgaactcggc ttcatctccc gcgcgccgcg ttgggcggtt gcccacaaat tccctgccga agaagccctg accattgtcg aagcgattga cgtgcaaatc ggcaggacgg gcgcggtaac gccggttgcc cgcctgcaac ccgtattcgt cggcggcgta accgttacca acgccaccct gcacaatcag gacgaagtat cgcgcaaaga cgtgcgcgtc ggcgataccg tcgtcgtgcg ccgcgccgga gacgtgattc ccgaagtcgt gcgcgtgatt ttcgaacgcc gcccgatgca ggaaaccgcc gttgccgttt cagacggcat cgggcatcag caggacgacc tgtttgccga aacaccgtcc gccaagcaaa ccgaatccgt tccgctccat aagccctacc gcctgccggc ccgctgcccc atctgccgca gcgaaatcga acgcgaagaa ggcgaagccg tcgcccgatg cagcggcggt atgctttgtc aggcacaacg cgcgcaaggc ttaatccact tcgcctcgcg caaagcgatg gacatcgacg gcttgggtga aaaacaaatc gaacagctgg tcgcccaaga cctcgtccgc catttcgccg acctctaccg catcgatatt ccgaccctgc aaaaaatgaa ggaaacggcg gataaagggt catctgaaaa cgaaaacggc gacgcagaaa cggtttcagg cgacctgtct aaatacaata cccaaaacgg caaaaaacaa ccgaccaagt gggcgcaaaa catcctcgca ggcatagaat ccggcaaaac gcccgaactt gcccgcttcc tgttcgcgct cggcatccgc cacgtcggcg aacgcaccgc caaaacgctg gcgcaggcat tcggcacatt ggaacgcgtc cgccgcgccc ccgaacccgt cctcgcctgc ctgcccgaca tcggcacggt agtggcgcgt tccattgccc acttcttcgc ccaagccgaa cagcaggcga tgatagacga gctgctcgcc gcaggcgttg ccccgcaagc ccaagccgtc agcctgcctg ccgcacaata tgccgggccg caacgctgga ttacccgcct gcccggtttc aaaatcagcg aaaacaaagc ccaagcctta tgggaactcg ccggacaaag tatagaagga ctgcaaaacg acaaagccct ccctgccgac tggcaggcgt ggcgcagcaa agcgcaaaac accgccctgc tcgaaaacct aaaaaccttc ttcgcgcaaa tgccgtctga agacgaggcg gcgcaaggtt cagacggcat caataaagcc gtggcaggca aaacctttgt gttaaccggc accctgccca ccttcaaacg cgaccaagcc caagccctaa tcgaagccgc aggcggcaaa gtgtccggca gcgtgtccaa aaaaaccgac tacgtcgtcg ccggagaagc cgcgggcagc aagctggaaa aagccaatgc cttgggcgtt tccgtcctca gcgaagccga actgcttacc ctgctctgct ga
Details for NegoA.17946.a.B1.GE39725
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 91kDa
OBSERVED MW: 91kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 86
PERCENT COVERAGE: 37
Validated AA Sequence
MAHHHHHHMN PTAQRIHELT DLLNRYAYEY YTLDAPSIPD AEYDRLFREL EALERNHPEL KLPDSPTQRV GGEPLAGFAE VRHEVPMLSL TNAFSPQDEN GVFDHAEMYA FDQRVRDGLD GGNPEYVIEP KFDGLAISLL YRDGVLVQAA TRGDGTTGED VTRNVKTVSN IPLRLHGENV PELIEVRGEV LMLKADFAAL NQRQTENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSVARQQXGL IAEEHIQELA YFQALGFSLP XXXXGXXKXI GXXXXXXXXX XXKXXXXXLX XXXXXXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatgaac ccgaccgcac aacgcataca cgaactcacc gacctcctca accgctacgc ctacgaatac tacaccctcg acgcgcccag catacccgat gccgaatacg accgcctgtt ccgcgaactc gaagcgttgg aacgaaacca tcccgagctc aagctgcccg acagcccgac ccagcgcgtc ggcggcgagc ctttggcagg ctttgccgaa gtgcgccacg aagtgccgat gctgtcgctg accaacgcct tttccccgca agatgaaaac ggcgtgttcg accatgccga aatgtacgcc ttcgaccaac gcgtgcgcga cggcttggac ggcggcaatc ccgaatacgt tatcgaaccc aaattcgacg gcctcgccat cagcctgctc taccgcgacg gcgtattggt gcaggcggca acgcgcggcg acggcacgac gggcgaagac gttacccgaa acgtcaaaac cgtatccaac atccccctgc ggctgcacgg cgaaaacgtg cccgaactca tcgaagtacg cggcgaagtg ctgatgctca aagccgattt tgccgcttta aaccaacgtc agaccgaaaa cgggcaaaaa cctttcgcca atccgcgcaa tgccgccgcc ggcagcctgc gccaactcga ttcgcgcatc accgcgcaac gcaaactgca ctttttcccc tactccgtcg cccgccagca angcggtttg attgcggaag aacacatcca agaactcgcc tatttccaag cactcggctt cagtctgccn nangnnnnnn nnggcnnnnt caaannnatc ggcgnnntnn nnnngtnnnn nnnnnnnnnn cnnnnaaaan nnnnnnnnnn nnnnctnnnn nntcnnnnnn nnnnnnnngn nnnnnncc
Expected Protein Sequence
MAHHHHHHMN PTAQRIHELT DLLNRYAYEY YTLDAPSIPD AEYDRLFREL EALERNHPEL KLPDSPTQRV GGEPLAGFAE VRHEVPMLSL TNAFSPQDEN GVFDHAEMYA FDQRVRDGLD GGNPEYVIEP KFDGLAISLL YRDGVLVQAA TRGDGTTGED VTRNVKTVSN IPLRLHGENV PELIEVRGEV LMLKADFAGL NKRQAENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSIARQQGGF EAEEHIQELA YFQELGFSLP NGNFGCFKNI GEVLAFYEHM QQKRPELPYE IDGMVVKVNS LAQQRELGFI SRAPRWAVAH KFPAEEALTI VEAIDVQIGR TGAVTPVARL QPVFVGGVTV TNATLHNQDE VSRKDVRVGD TVVVRRAGDV IPEVVRVIFE RRPMQETAVA VSDGIGHQQD DLFAETPSAK QTESVPLHKP YRLPARCPIC RSEIEREEGE AVARCSGGML CQAQRAQGLI HFASRKAMDI DGLGEKQIEQ LVAQDLVRHF ADLYRIDIPT LQKMKETADK GSSENENGDA ETVSGDLSKY NTQNGKKQPT KWAQNILAGI ESGKTPELAR FLFALGIRHV GERTAKTLAQ AFGTLERVRR APEPVLACLP DIGTVVARSI AHFFAQAEQQ AMIDELLAAG VAPQAQAVSL PAAQYAGPQR WITRLPGFKI SENKAQALWE LAGQSIEGLQ NDKALPADWQ AWRSKAQNTA LLENLKTFFA QMPSEDEAAQ GSDGINKAVA GKTFVLTGTL PTFKRDQAQA LIEAAGGKVS GSVSKKTDYV VAGEAAGSKL EKANALGVSV LSEAELLTLL C
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc cgaccgcaca acgcatacac gaactcaccg acctcctcaa ccgctacgcc tacgaatact acaccctcga cgcgcccagc atacccgatg ccgaatacga ccgcctgttc cgcgaactcg aagcgttgga acgaaaccat cccgagctca agctgcccga cagcccgacc cagcgcgtcg gcggcgagcc tttggcaggc tttgccgaag tgcgccacga agtgccgatg ctgtcgctga ccaacgcctt ttccccgcaa gatgaaaacg gcgtgttcga ccatgccgaa atgtacgcct tcgaccaacg cgtgcgcgac ggcttggacg gcggcaatcc cgaatacgtt atcgaaccca aattcgacgg cctcgccatc agcctgctct accgcgacgg cgtattggtg caggcggcaa cgcgcggcga cggcacgacg ggcgaagacg ttacccgaaa cgtcaaaacc gtatccaaca tccccctgcg gctgcacggc gaaaacgtgc ccgaactcat cgaggtacgc ggcgaagtgc tgatgctcaa agccgacttt gccgggctca ataaacgcca agccgaaaac gggcaaaaac cctttgccaa tccgcgcaac gccgccgccg gcagcctgcg ccaactcgat tcgcgcatca ccgcgcaacg caaactgcac tttttcccct attcgattgc ccgtcagcaa ggcggtttcg aagctgaaga acacatccaa gaactcgcct atttccaaga actcggcttc agtctgccca atggcaattt cggctgtttc aaaaatatcg gcgaagtatt ggcgttttac gaacacatgc agcaaaaacg ccccgaactg ccctacgaaa tcgacggcat ggtggtcaaa gtcaacagct tggcgcaaca gcgcgaactc ggcttcatct cccgcgcgcc gcgttgggcg gttgcccaca aattccctgc cgaagaagcc ctgaccattg tcgaagcgat tgacgtgcaa atcggcagga cgggcgcggt aacgccggtt gcccgcctgc aacccgtatt cgtcggcggc gtaaccgtta ccaacgccac cctgcacaat caggacgaag tatcgcgcaa agacgtgcgc gtcggcgata ccgtcgtcgt gcgccgcgcc ggagacgtga ttcccgaagt cgtgcgcgtg attttcgaac gccgcccgat gcaggaaacc gccgttgccg tttcagacgg catcgggcat cagcaggacg acctgtttgc cgaaacaccg tccgccaagc aaaccgaatc cgttccgctc cataagccct accgcctgcc ggcccgctgc cccatctgcc gcagcgaaat cgaacgcgaa gaaggcgaag ccgtcgcccg atgcagcggc ggtatgcttt gtcaggcaca acgcgcgcaa ggcttaatcc acttcgcctc gcgcaaagcg atggacatcg acggcttggg tgaaaaacaa atcgaacagc tggtcgccca agacctcgtc cgccatttcg ccgacctcta ccgcatcgat attccgaccc tgcaaaaaat gaaggaaacg gcggataaag ggtcatctga aaacgaaaac ggcgacgcag aaacggtttc aggcgacctg tctaaataca atacccaaaa cggcaaaaaa caaccgacca agtgggcgca aaacatcctc gcaggcatag aatccggcaa aacgcccgaa cttgcccgct tcctgttcgc gctcggcatc cgccacgtcg gcgaacgcac cgccaaaacg ctggcgcagg cattcggcac attggaacgc gtccgccgcg cccccgaacc cgtcctcgcc tgcctgcccg acatcggcac ggtagtggcg cgttccattg cccacttctt cgcccaagcc gaacagcagg cgatgataga cgagctgctc gccgcaggcg ttgccccgca agcccaagcc gtcagcctgc ctgccgcaca atatgccggg ccgcaacgct ggattacccg cctgcccggt ttcaaaatca gcgaaaacaa agcccaagcc ttatgggaac tcgccggaca aagtatagaa ggactgcaaa acgacaaagc cctccctgcc gactggcagg cgtggcgcag caaagcgcaa aacaccgccc tgctcgaaaa cctaaaaacc ttcttcgcgc aaatgccgtc tgaagacgag gcggcgcaag gttcagacgg catcaataaa gccgtggcag gcaaaacctt tgtgttaacc ggcaccctgc ccaccttcaa acgcgaccaa gcccaagccc taatcgaagc cgcaggcggc aaagtgtccg gcagcgtgtc caaaaaaacc gactacgtcg tcgccggaga agccgcgggc agcaagctgg aaaaagccaa tgccttgggc gtttccgtcc tcagcgaagc cgaactgctt accctgctct gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for NegoA.17946.a.B2.GE40396
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 61kDa
OBSERVED MW: 61kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN PTAQRIHELT DLLNRYAYEY YTLDAPSIPD AEYDRLFREL EALERNHPEL KLPDSPTQRV GGEPLAGFAE VRHEVPMLSL TNAFSPQDEN GVFDHAEMYA FDQRVRDGLD GGNPEYVIEP KFDGLAISLL YRDGVLVQAA TRGDGTTGED VTRNVKTVSN IPLRLHGENV PELIEVRGEV LMLKADFAGL NKRQAENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSIARQQGGF EAEEHIQELA YFQELGFSLP NGNFGCFKNI GEVLAFYEHM QQKRPELPYE IDGMVVKVNS LAQQRELGFI SRAPRWAVAH KFPAEEALTI VEAIDVQIGR TGAVTPVARL QPVFVGGVTV TNATLHNQDE VSRKDVRVGD TVVVRRAGDV IPEVVRVIFE RRPMQETAVA VSDGIGHQQD DLFAETPSAK QTESVPLHKP YRLPARCPIC RSEIEREEGE AVARCSGGML CQAQRAQGLI HFASRKAMDI DGLGEKQIEQ LVAQDLVRHF ADLYRIDIPT LQKMKETADK GSS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc cgaccgcaca acgcatacac gaactcaccg acctcctcaa ccgctacgcc tacgaatact acaccctcga cgcgcccagc atacccgatg ccgaatacga ccgcctgttc cgcgaactcg aagcgttgga acgaaaccat cccgagctca agctgcccga cagcccgacc cagcgcgtcg gcggcgagcc tttggcaggc tttgccgaag tgcgccacga agtgccgatg ctgtcgctga ccaacgcctt ttccccgcaa gatgaaaacg gcgtgttcga ccatgccgaa atgtacgcct tcgaccaacg cgtgcgcgac ggcttggacg gcggcaatcc cgaatacgtt atcgaaccca aattcgacgg cctcgccatc agcctgctct accgcgacgg cgtattggtg caggcggcaa cgcgcggcga cggcacgacg ggcgaagacg ttacccgaaa cgtcaaaacc gtatccaaca tccccctgcg gctgcacggc gaaaacgtgc ccgaactcat cgaggtacgc ggcgaagtgc tgatgctcaa agccgacttt gccgggctca ataaacgcca agccgaaaac gggcaaaaac cctttgccaa tccgcgcaac gccgccgccg gcagcctgcg ccaactcgat tcgcgcatca ccgcgcaacg caaactgcac tttttcccct attcgattgc ccgtcagcaa ggcggtttcg aagctgaaga acacatccaa gaactcgcct atttccaaga actcggcttc agtctgccca atggcaattt cggctgtttc aaaaatatcg gcgaagtatt ggcgttttac gaacacatgc agcaaaaacg ccccgaactg ccctacgaaa tcgacggcat ggtggtcaaa gtcaacagct tggcgcaaca gcgcgaactc ggcttcatct cccgcgcgcc gcgttgggcg gttgcccaca aattccctgc cgaagaagcc ctgaccattg tcgaagcgat tgacgtgcaa atcggcagga cgggcgcggt aacgccggtt gcccgcctgc aacccgtatt cgtcggcggc gtaaccgtta ccaacgccac cctgcacaat caggacgaag tatcgcgcaa agacgtgcgc gtcggcgata ccgtcgtcgt gcgccgcgcc ggagacgtga ttcccgaagt cgtgcgcgtg attttcgaac gccgcccgat gcaggaaacc gccgttgccg tttcagacgg catcgggcat cagcaggacg acctgtttgc cgaaacaccg tccgccaagc aaaccgaatc cgttccgctc cataagccct accgcctgcc ggcccgctgc cccatctgcc gcagcgaaat cgaacgcgaa gaaggcgaag ccgtcgcccg atgcagcggc ggtatgcttt gtcaggcaca acgcgcgcaa ggcttaatcc acttcgcctc gcgcaaagcg atggacatcg acggcttggg tgaaaaacaa atcgaacagc tggtcgccca agacctcgtc cgccatttcg ccgacctcta ccgcatcgat attccgaccc tgcaaaaaat gaaggaaacg gcggataaag ggtcatcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for NegoA.17946.a.B4.GE40398
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMN PTAQRIHELT DLLNRYAYEY YTLDAPSIPD AEYDRLFREL EALERNHPEL KLPDSPTQRV GGEPLAGFAE VRHEVPMLSL TNAFSPQDEN GVFDHAEMYA FDQRVRDGLD GGNPEYVIEP KFDGLAISLL YRDGVLVQAA TRGDGTTGED VTRNVKTVSN IPLRLHGENV PELIEVRGEV LMLKADFAAL NQRQTENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSVARQQGGL IAEEHIQELA YFQALGFSLP NGNFGCFKNI GEVLAFYEHM QQKRPELPYE IDGTVVKVNS LAQQHELGFI SRAPRWAVAH KFPAEEALTI VEAIDVQIGR TGAVTPVARL QPVFVGGVTV TNATLHNQDE VSR
Validated NT Sequence
ttgcgcgata cttcgtcctg attgtgcagg gtggcgttgg taacggttac gccgccgacg aatacgggtt gcaggcgggc aaccggcgtt accgcgcccg tcctgccgat ttgcacgtca atcgcttcga caatggtcag ggcttcttcg gcagggaatt tgtgggcaac cgcccagcgc ggcgcgcggg agatgaagcc gagttcgtgc tgttgcgcca agctgttgac tttgacgacc gtgccgtcga tttcgtaggg cagttcgggg cgtttttgct gcatgtgttc gtaaaacgcc aatacttcgc cgatattttt gaaacagccg aaattgccat tgggcagact gaagccgagt gcttggaaat aggcgagttc ttggatgtgt tcttccgcaa tcaaaccgcc ttgctggcgg gcgacggagt aggggaaaaa gtgcagtttg cgttgcgcgg tgatgcgcga atcgagttgg cgcaggctgc cggcggcggc attgcgcgga ttggcgaaag gtttttgccc gttttcggtc tgacgttggt ttaaagcggc aaaatcggct ttgagcatca gcacttcgcc gcgtacttcg atgagttcgg gcacgttttc gccgtgcagc cgcaggggga tgttggatac ggttttgacg tttcgggtaa cgtcttcgcc cgtcgtgccg tcgccgcgcg ttgccgcctg caccaatacg ccgtcgcggt agagcaggct gatggcgagg ccgtcgaatt tgggttcgat aacgtattcg ggattgccgc cgtccaagcc gtcgcgcacg cgttggtcga aggcgtacat ttcggcatgg tcgaacacgc cgttttcatc ttgcggggaa aaggcgttgg tcagcgacag catcggcact tcgtggcgca cttcggcaaa gcctgccaaa ggctcgccgc cgacgcgctg ggtcgggctg tcgggcagct tgagctcggg atggtttcgt tccaacgctt cgagttcgcg gaacaggcgg tcgtattcgg catcgggtat gctgggcgcg tcgagggtgt agtattcgta ggcgtagcgg ttgaggaggt cggtgagttc gtgtatgcgt tgtgcggtcg ggttcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMN PTAQRIHELT DLLNRYAYEY YTLDAPSIPD AEYDRLFREL EALERNHPEL KLPDSPTQRV GGEPLAGFAE VRHEVPMLSL TNAFSPQDEN GVFDHAEMYA FDQRVRDGLD GGNPEYVIEP KFDGLAISLL YRDGVLVQAA TRGDGTTGED VTRNVKTVSN IPLRLHGENV PELIEVRGEV LMLKADFAGL NKRQAENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSIARQQGGF EAEEHIQELA YFQELGFSLP NGNFGCFKNI GEVLAFYEHM QQKRPELPYE IDGMVVKVNS LAQQRELGFI SRAPRWAVAH KFPAEEALTI VEAIDVQIGR TGAVTPVARL QPVFVGGVTV TNATLHNQDE VSRKDVRVGD T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc cgaccgcaca acgcatacac gaactcaccg acctcctcaa ccgctacgcc tacgaatact acaccctcga cgcgcccagc atacccgatg ccgaatacga ccgcctgttc cgcgaactcg aagcgttgga acgaaaccat cccgagctca agctgcccga cagcccgacc cagcgcgtcg gcggcgagcc tttggcaggc tttgccgaag tgcgccacga agtgccgatg ctgtcgctga ccaacgcctt ttccccgcaa gatgaaaacg gcgtgttcga ccatgccgaa atgtacgcct tcgaccaacg cgtgcgcgac ggcttggacg gcggcaatcc cgaatacgtt atcgaaccca aattcgacgg cctcgccatc agcctgctct accgcgacgg cgtattggtg caggcggcaa cgcgcggcga cggcacgacg ggcgaagacg ttacccgaaa cgtcaaaacc gtatccaaca tccccctgcg gctgcacggc gaaaacgtgc ccgaactcat cgaggtacgc ggcgaagtgc tgatgctcaa agccgacttt gccgggctca ataaacgcca agccgaaaac gggcaaaaac cctttgccaa tccgcgcaac gccgccgccg gcagcctgcg ccaactcgat tcgcgcatca ccgcgcaacg caaactgcac tttttcccct attcgattgc ccgtcagcaa ggcggtttcg aagctgaaga acacatccaa gaactcgcct atttccaaga actcggcttc agtctgccca atggcaattt cggctgtttc aaaaatatcg gcgaagtatt ggcgttttac gaacacatgc agcaaaaacg ccccgaactg ccctacgaaa tcgacggcat ggtggtcaaa gtcaacagct tggcgcaaca gcgcgaactc ggcttcatct cccgcgcgcc gcgttgggcg gttgcccaca aattccctgc cgaagaagcc ctgaccattg tcgaagcgat tgacgtgcaa atcggcagga cgggcgcggt aacgccggtt gcccgcctgc aacccgtatt cgtcggcggc gtaaccgtta ccaacgccac cctgcacaat caggacgaag tatcgcgcaa agacgtgcgc gtcggcgata cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for NegoA.17946.a.B6.GE40399
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHSN IPLRLHGENV PELIEVRGEV LMLKADFAGL NKRQAENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSIARQQGGF EAEEHIQELA YFQELGFSLP NGNFGCFKNI GEVLAFYEHM QQKRPELPYE IDGMVVKVNS LAQQRELGFI SRAPRWAVAH KFPAEEALTI VEAIDVQIGR TGAVTPVARL QPVFVGGVTV TNATLHNQDE VSRKDVRVGD TVVVRRAGDV IPEVVRVIFE RRPMQETAVA VSDGIGHQQD DLFAETPSAK QTESVPLHKP YRLPARCPIC RSEIEREEGE AVARCSGGML CQAQRAQGLI HFASRKAMDI DGLGEKQIEQ LVAQDLVRHF ADLYRIDIPT LQKMKETADK GSS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccaaca tccccctgcg gctgcacggc gaaaacgtgc ccgaactcat cgaggtacgc ggcgaagtgc tgatgctcaa agccgacttt gccgggctca ataaacgcca agccgaaaac gggcaaaaac cctttgccaa tccgcgcaac gccgccgccg gcagcctgcg ccaactcgat tcgcgcatca ccgcgcaacg caaactgcac tttttcccct attcgattgc ccgtcagcaa ggcggtttcg aagctgaaga acacatccaa gaactcgcct atttccaaga actcggcttc agtctgccca atggcaattt cggctgtttc aaaaatatcg gcgaagtatt ggcgttttac gaacacatgc agcaaaaacg ccccgaactg ccctacgaaa tcgacggcat ggtggtcaaa gtcaacagct tggcgcaaca gcgcgaactc ggcttcatct cccgcgcgcc gcgttgggcg gttgcccaca aattccctgc cgaagaagcc ctgaccattg tcgaagcgat tgacgtgcaa atcggcagga cgggcgcggt aacgccggtt gcccgcctgc aacccgtatt cgtcggcggc gtaaccgtta ccaacgccac cctgcacaat caggacgaag tatcgcgcaa agacgtgcgc gtcggcgata ccgtcgtcgt gcgccgcgcc ggagacgtga ttcccgaagt cgtgcgcgtg attttcgaac gccgcccgat gcaggaaacc gccgttgccg tttcagacgg catcgggcat cagcaggacg acctgtttgc cgaaacaccg tccgccaagc aaaccgaatc cgttccgctc cataagccct accgcctgcc ggcccgctgc cccatctgcc gcagcgaaat cgaacgcgaa gaaggcgaag ccgtcgcccg atgcagcggc ggtatgcttt gtcaggcaca acgcgcgcaa ggcttaatcc acttcgcctc gcgcaaagcg atggacatcg acggcttggg tgaaaaacaa atcgaacagc tggtcgccca agacctcgtc cgccatttcg ccgacctcta ccgcatcgat attccgaccc tgcaaaaaat gaaggaaacg gcggataaag ggtcatcttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for NegoA.17946.a.B7.GE40400
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 73kDa
OBSERVED MW: 73kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHSN IPLRLHGENV PELIEVRGEV LMLKADFAGL NKRQAENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSIARQQGGF EAEEHIQELA YFQELGFSLP NGNFGCFKNI GEVLAFYEHM QQKRPELPYE IDGMVVKVNS LAQQRELGFI SRAPRWAVAH KFPAEEALTI VEAIDVQIGR TGAVTPVARL QPVFVGGVTV TNATLHNQDE VSRKDVRVGD TVVVRRAGDV IPEVVRVIFE RRPMQETAVA VSDGIGHQQD DLFAETPSAK QTESVPLHKP YRLPARCPIC RSEIEREEGE AVARCSGGML CQAQRAQGLI HFASRKAMDI DGLGEKQIEQ LVAQDLVRHF ADLYRIDIPT LQKMKETADK GSSENENGDA ETVSGDLSKY NTQNGKKQPT KWAQNILAGI ESGKTPELAR FLFALGIRHV GERTAKTLAQ AFGTLERVRR APEPVLACLP DIGTVVARSI AHFFAQAEQQ AMIDELLAAG VAPQAQAVSL PAAQYAGPQR WITRLPGFKI SENKAQALWE LAGQSIEGLQ NDKALPADWQ AWRSKAQNTA LLENLKTFFA QMPSEDEAAQ GSDGINKAVA GKTFVLTGTL PTFKRDQAQA LIEAAGGKVS GSVSKKTDYV VAGEAAGSKL EKANALGVSV LSEAELLTLL C
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattccaaca tccccctgcg gctgcacggc gaaaacgtgc ccgaactcat cgaggtacgc ggcgaagtgc tgatgctcaa agccgacttt gccgggctca ataaacgcca agccgaaaac gggcaaaaac cctttgccaa tccgcgcaac gccgccgccg gcagcctgcg ccaactcgat tcgcgcatca ccgcgcaacg caaactgcac tttttcccct attcgattgc ccgtcagcaa ggcggtttcg aagctgaaga acacatccaa gaactcgcct atttccaaga actcggcttc agtctgccca atggcaattt cggctgtttc aaaaatatcg gcgaagtatt ggcgttttac gaacacatgc agcaaaaacg ccccgaactg ccctacgaaa tcgacggcat ggtggtcaaa gtcaacagct tggcgcaaca gcgcgaactc ggcttcatct cccgcgcgcc gcgttgggcg gttgcccaca aattccctgc cgaagaagcc ctgaccattg tcgaagcgat tgacgtgcaa atcggcagga cgggcgcggt aacgccggtt gcccgcctgc aacccgtatt cgtcggcggc gtaaccgtta ccaacgccac cctgcacaat caggacgaag tatcgcgcaa agacgtgcgc gtcggcgata ccgtcgtcgt gcgccgcgcc ggagacgtga ttcccgaagt cgtgcgcgtg attttcgaac gccgcccgat gcaggaaacc gccgttgccg tttcagacgg catcgggcat cagcaggacg acctgtttgc cgaaacaccg tccgccaagc aaaccgaatc cgttccgctc cataagccct accgcctgcc ggcccgctgc cccatctgcc gcagcgaaat cgaacgcgaa gaaggcgaag ccgtcgcccg atgcagcggc ggtatgcttt gtcaggcaca acgcgcgcaa ggcttaatcc acttcgcctc gcgcaaagcg atggacatcg acggcttggg tgaaaaacaa atcgaacagc tggtcgccca agacctcgtc cgccatttcg ccgacctcta ccgcatcgat attccgaccc tgcaaaaaat gaaggaaacg gcggataaag ggtcatctga aaacgaaaac ggcgacgcag aaacggtttc aggcgacctg tctaaataca atacccaaaa cggcaaaaaa caaccgacca agtgggcgca aaacatcctc gcaggcatag aatccggcaa aacgcccgaa cttgcccgct tcctgttcgc gctcggcatc cgccacgtcg gcgaacgcac cgccaaaacg ctggcgcagg cattcggcac attggaacgc gtccgccgcg cccccgaacc cgtcctcgcc tgcctgcccg acatcggcac ggtagtggcg cgttccattg cccacttctt cgcccaagcc gaacagcagg cgatgataga cgagctgctc gccgcaggcg ttgccccgca agcccaagcc gtcagcctgc ctgccgcaca atatgccggg ccgcaacgct ggattacccg cctgcccggt ttcaaaatca gcgaaaacaa agcccaagcc ttatgggaac tcgccggaca aagtatagaa ggactgcaaa acgacaaagc cctccctgcc gactggcagg cgtggcgcag caaagcgcaa aacaccgccc tgctcgaaaa cctaaaaacc ttcttcgcgc aaatgccgtc tgaagacgag gcggcgcaag gttcagacgg catcaataaa gccgtggcag gcaaaacctt tgtgttaacc ggcaccctgc ccaccttcaa acgcgaccaa gcccaagccc taatcgaagc cgcaggcggc aaagtgtccg gcagcgtgtc caaaaaaacc gactacgtcg tcgccggaga agccgcgggc agcaagctgg aaaaagccaa tgccttgggc gtttccgtcc tcagcgaagc cgaactgctt accctgctct gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for NegoA.17946.a.B4.PS38107
PURIFICATION DATe: 11/8/2016
CONCENTRATION: 40.11mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN PTAQRIHELT DLLNRYAYEY YTLDAPSIPD AEYDRLFREL EALERNHPEL KLPDSPTQRV GGEPLAGFAE VRHEVPMLSL TNAFSPQDEN GVFDHAEMYA FDQRVRDGLD GGNPEYVIEP KFDGLAISLL YRDGVLVQAA TRGDGTTGED VTRNVKTVSN IPLRLHGENV PELIEVRGEV LMLKADFAAL NQRQTENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSVARQQGGL IAEEHIQELA YFQALGFSLP NGNFGCFKNI GEVLAFYEHM QQKRPELPYE IDGTVVKVNS LAQQHELGFI SRAPRWAVAH KFPAEEALTI VEAIDVQIGR TGAVTPVARL QPVFVGGVTV TNATLHNQDE VSR
Validated NT Sequence
ttgcgcgata cttcgtcctg attgtgcagg gtggcgttgg taacggttac gccgccgacg aatacgggtt gcaggcgggc aaccggcgtt accgcgcccg tcctgccgat ttgcacgtca atcgcttcga caatggtcag ggcttcttcg gcagggaatt tgtgggcaac cgcccagcgc ggcgcgcggg agatgaagcc gagttcgtgc tgttgcgcca agctgttgac tttgacgacc gtgccgtcga tttcgtaggg cagttcgggg cgtttttgct gcatgtgttc gtaaaacgcc aatacttcgc cgatattttt gaaacagccg aaattgccat tgggcagact gaagccgagt gcttggaaat aggcgagttc ttggatgtgt tcttccgcaa tcaaaccgcc ttgctggcgg gcgacggagt aggggaaaaa gtgcagtttg cgttgcgcgg tgatgcgcga atcgagttgg cgcaggctgc cggcggcggc attgcgcgga ttggcgaaag gtttttgccc gttttcggtc tgacgttggt ttaaagcggc aaaatcggct ttgagcatca gcacttcgcc gcgtacttcg atgagttcgg gcacgttttc gccgtgcagc cgcaggggga tgttggatac ggttttgacg tttcgggtaa cgtcttcgcc cgtcgtgccg tcgccgcgcg ttgccgcctg caccaatacg ccgtcgcggt agagcaggct gatggcgagg ccgtcgaatt tgggttcgat aacgtattcg ggattgccgc cgtccaagcc gtcgcgcacg cgttggtcga aggcgtacat ttcggcatgg tcgaacacgc cgttttcatc ttgcggggaa aaggcgttgg tcagcgacag catcggcact tcgtggcgca cttcggcaaa gcctgccaaa ggctcgccgc cgacgcgctg ggtcgggctg tcgggcagct tgagctcggg atggtttcgt tccaacgctt cgagttcgcg gaacaggcgg tcgtattcgg catcgggtat gctgggcgcg tcgagggtgt agtattcgta ggcgtagcgg ttgaggaggt cggtgagttc gtgtatgcgt tgtgcggtcg ggttcatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMN PTAQRIHELT DLLNRYAYEY YTLDAPSIPD AEYDRLFREL EALERNHPEL KLPDSPTQRV GGEPLAGFAE VRHEVPMLSL TNAFSPQDEN GVFDHAEMYA FDQRVRDGLD GGNPEYVIEP KFDGLAISLL YRDGVLVQAA TRGDGTTGED VTRNVKTVSN IPLRLHGENV PELIEVRGEV LMLKADFAGL NKRQAENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSIARQQGGF EAEEHIQELA YFQELGFSLP NGNFGCFKNI GEVLAFYEHM QQKRPELPYE IDGMVVKVNS LAQQRELGFI SRAPRWAVAH KFPAEEALTI VEAIDVQIGR TGAVTPVARL QPVFVGGVTV TNATLHNQDE VSRKDVRVGD T
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC CGACCGCACA ACGCATACAC GAACTCACCG ACCTCCTCAA CCGCTACGCC TACGAATACT ACACCCTCGA CGCGCCCAGC ATACCCGATG CCGAATACGA CCGCCTGTTC CGCGAACTCG AAGCGTTGGA ACGAAACCAT CCCGAGCTCA AGCTGCCCGA CAGCCCGACC CAGCGCGTCG GCGGCGAGCC TTTGGCAGGC TTTGCCGAAG TGCGCCACGA AGTGCCGATG CTGTCGCTGA CCAACGCCTT TTCCCCGCAA GATGAAAACG GCGTGTTCGA CCATGCCGAA ATGTACGCCT TCGACCAACG CGTGCGCGAC GGCTTGGACG GCGGCAATCC CGAATACGTT ATCGAACCCA AATTCGACGG CCTCGCCATC AGCCTGCTCT ACCGCGACGG CGTATTGGTG CAGGCGGCAA CGCGCGGCGA CGGCACGACG GGCGAAGACG TTACCCGAAA CGTCAAAACC GTATCCAACA TCCCCCTGCG GCTGCACGGC GAAAACGTGC CCGAACTCAT CGAGGTACGC GGCGAAGTGC TGATGCTCAA AGCCGACTTT GCCGGGCTCA ATAAACGCCA AGCCGAAAAC GGGCAAAAAC CCTTTGCCAA TCCGCGCAAC GCCGCCGCCG GCAGCCTGCG CCAACTCGAT TCGCGCATCA CCGCGCAACG CAAACTGCAC TTTTTCCCCT ATTCGATTGC CCGTCAGCAA GGCGGTTTCG AAGCTGAAGA ACACATCCAA GAACTCGCCT ATTTCCAAGA ACTCGGCTTC AGTCTGCCCA ATGGCAATTT CGGCTGTTTC AAAAATATCG GCGAAGTATT GGCGTTTTAC GAACACATGC AGCAAAAACG CCCCGAACTG CCCTACGAAA TCGACGGCAT GGTGGTCAAA GTCAACAGCT TGGCGCAACA GCGCGAACTC GGCTTCATCT CCCGCGCGCC GCGTTGGGCG GTTGCCCACA AATTCCCTGC CGAAGAAGCC CTGACCATTG TCGAAGCGAT TGACGTGCAA ATCGGCAGGA CGGGCGCGGT AACGCCGGTT GCCCGCCTGC AACCCGTATT CGTCGGCGGC GTAACCGTTA CCAACGCCAC CCTGCACAAT CAGGACGAAG TATCGCGCAA AGACGTGCGC GTCGGCGATA CCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for NegoA.17946.a.B1.PW37842
PURIFICATION DATe: 1/27/2016
CONCENTRATION: 24mg/ml
OBSERVED MW: 90kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 86
PERCENT COVERAGE: 37
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN PTAQRIHELT DLLNRYAYEY YTLDAPSIPD AEYDRLFREL EALERNHPEL KLPDSPTQRV GGEPLAGFAE VRHEVPMLSL TNAFSPQDEN GVFDHAEMYA FDQRVRDGLD GGNPEYVIEP KFDGLAISLL YRDGVLVQAA TRGDGTTGED VTRNVKTVSN IPLRLHGENV PELIEVRGEV LMLKADFAAL NQRQTENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSVARQQXGL IAEEHIQELA YFQALGFSLP XXXXGXXKXI GXXXXXXXXX XXKXXXXXLX XXXXXXXXX
Validated NT Sequence
atggctcacc accaccacca ccatatgaac ccgaccgcac aacgcataca cgaactcacc gacctcctca accgctacgc ctacgaatac tacaccctcg acgcgcccag catacccgat gccgaatacg accgcctgtt ccgcgaactc gaagcgttgg aacgaaacca tcccgagctc aagctgcccg acagcccgac ccagcgcgtc ggcggcgagc ctttggcagg ctttgccgaa gtgcgccacg aagtgccgat gctgtcgctg accaacgcct tttccccgca agatgaaaac ggcgtgttcg accatgccga aatgtacgcc ttcgaccaac gcgtgcgcga cggcttggac ggcggcaatc ccgaatacgt tatcgaaccc aaattcgacg gcctcgccat cagcctgctc taccgcgacg gcgtattggt gcaggcggca acgcgcggcg acggcacgac gggcgaagac gttacccgaa acgtcaaaac cgtatccaac atccccctgc ggctgcacgg cgaaaacgtg cccgaactca tcgaagtacg cggcgaagtg ctgatgctca aagccgattt tgccgcttta aaccaacgtc agaccgaaaa cgggcaaaaa cctttcgcca atccgcgcaa tgccgccgcc ggcagcctgc gccaactcga ttcgcgcatc accgcgcaac gcaaactgca ctttttcccc tactccgtcg cccgccagca angcggtttg attgcggaag aacacatcca agaactcgcc tatttccaag cactcggctt cagtctgccn nangnnnnnn nnggcnnnnt caaannnatc ggcgnnntnn nnnngtnnnn nnnnnnnnnn cnnnnaaaan nnnnnnnnnn nnnnctnnnn nntcnnnnnn nnnnnnnngn nnnnnncc
Expressed Protein Sequence
MAHHHHHHMN PTAQRIHELT DLLNRYAYEY YTLDAPSIPD AEYDRLFREL EALERNHPEL KLPDSPTQRV GGEPLAGFAE VRHEVPMLSL TNAFSPQDEN GVFDHAEMYA FDQRVRDGLD GGNPEYVIEP KFDGLAISLL YRDGVLVQAA TRGDGTTGED VTRNVKTVSN IPLRLHGENV PELIEVRGEV LMLKADFAGL NKRQAENGQK PFANPRNAAA GSLRQLDSRI TAQRKLHFFP YSIARQQGGF EAEEHIQELA YFQELGFSLP NGNFGCFKNI GEVLAFYEHM QQKRPELPYE IDGMVVKVNS LAQQRELGFI SRAPRWAVAH KFPAEEALTI VEAIDVQIGR TGAVTPVARL QPVFVGGVTV TNATLHNQDE VSRKDVRVGD TVVVRRAGDV IPEVVRVIFE RRPMQETAVA VSDGIGHQQD DLFAETPSAK QTESVPLHKP YRLPARCPIC RSEIEREEGE AVARCSGGML CQAQRAQGLI HFASRKAMDI DGLGEKQIEQ LVAQDLVRHF ADLYRIDIPT LQKMKETADK GSSENENGDA ETVSGDLSKY NTQNGKKQPT KWAQNILAGI ESGKTPELAR FLFALGIRHV GERTAKTLAQ AFGTLERVRR APEPVLACLP DIGTVVARSI AHFFAQAEQQ AMIDELLAAG VAPQAQAVSL PAAQYAGPQR WITRLPGFKI SENKAQALWE LAGQSIEGLQ NDKALPADWQ AWRSKAQNTA LLENLKTFFA QMPSEDEAAQ GSDGINKAVA GKTFVLTGTL PTFKRDQAQA LIEAAGGKVS GSVSKKTDYV VAGEAAGSKL EKANALGVSV LSEAELLTLL C
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC CGACCGCACA ACGCATACAC GAACTCACCG ACCTCCTCAA CCGCTACGCC TACGAATACT ACACCCTCGA CGCGCCCAGC ATACCCGATG CCGAATACGA CCGCCTGTTC CGCGAACTCG AAGCGTTGGA ACGAAACCAT CCCGAGCTCA AGCTGCCCGA CAGCCCGACC CAGCGCGTCG GCGGCGAGCC TTTGGCAGGC TTTGCCGAAG TGCGCCACGA AGTGCCGATG CTGTCGCTGA CCAACGCCTT TTCCCCGCAA GATGAAAACG GCGTGTTCGA CCATGCCGAA ATGTACGCCT TCGACCAACG CGTGCGCGAC GGCTTGGACG GCGGCAATCC CGAATACGTT ATCGAACCCA AATTCGACGG CCTCGCCATC AGCCTGCTCT ACCGCGACGG CGTATTGGTG CAGGCGGCAA CGCGCGGCGA CGGCACGACG GGCGAAGACG TTACCCGAAA CGTCAAAACC GTATCCAACA TCCCCCTGCG GCTGCACGGC GAAAACGTGC CCGAACTCAT CGAGGTACGC GGCGAAGTGC TGATGCTCAA AGCCGACTTT GCCGGGCTCA ATAAACGCCA AGCCGAAAAC GGGCAAAAAC CCTTTGCCAA TCCGCGCAAC GCCGCCGCCG GCAGCCTGCG CCAACTCGAT TCGCGCATCA CCGCGCAACG CAAACTGCAC TTTTTCCCCT ATTCGATTGC CCGTCAGCAA GGCGGTTTCG AAGCTGAAGA ACACATCCAA GAACTCGCCT ATTTCCAAGA ACTCGGCTTC AGTCTGCCCA ATGGCAATTT CGGCTGTTTC AAAAATATCG GCGAAGTATT GGCGTTTTAC GAACACATGC AGCAAAAACG CCCCGAACTG CCCTACGAAA TCGACGGCAT GGTGGTCAAA GTCAACAGCT TGGCGCAACA GCGCGAACTC GGCTTCATCT CCCGCGCGCC GCGTTGGGCG GTTGCCCACA AATTCCCTGC CGAAGAAGCC CTGACCATTG TCGAAGCGAT TGACGTGCAA ATCGGCAGGA CGGGCGCGGT AACGCCGGTT GCCCGCCTGC AACCCGTATT CGTCGGCGGC GTAACCGTTA CCAACGCCAC CCTGCACAAT CAGGACGAAG TATCGCGCAA AGACGTGCGC GTCGGCGATA CCGTCGTCGT GCGCCGCGCC GGAGACGTGA TTCCCGAAGT CGTGCGCGTG ATTTTCGAAC GCCGCCCGAT GCAGGAAACC GCCGTTGCCG TTTCAGACGG CATCGGGCAT CAGCAGGACG ACCTGTTTGC CGAAACACCG TCCGCCAAGC AAACCGAATC CGTTCCGCTC CATAAGCCCT ACCGCCTGCC GGCCCGCTGC CCCATCTGCC GCAGCGAAAT CGAACGCGAA GAAGGCGAAG CCGTCGCCCG ATGCAGCGGC GGTATGCTTT GTCAGGCACA ACGCGCGCAA GGCTTAATCC ACTTCGCCTC GCGCAAAGCG ATGGACATCG ACGGCTTGGG TGAAAAACAA ATCGAACAGC TGGTCGCCCA AGACCTCGTC CGCCATTTCG CCGACCTCTA CCGCATCGAT ATTCCGACCC TGCAAAAAAT GAAGGAAACG GCGGATAAAG GGTCATCTGA AAACGAAAAC GGCGACGCAG AAACGGTTTC AGGCGACCTG TCTAAATACA ATACCCAAAA CGGCAAAAAA CAACCGACCA AGTGGGCGCA AAACATCCTC GCAGGCATAG AATCCGGCAA AACGCCCGAA CTTGCCCGCT TCCTGTTCGC GCTCGGCATC CGCCACGTCG GCGAACGCAC CGCCAAAACG CTGGCGCAGG CATTCGGCAC ATTGGAACGC GTCCGCCGCG CCCCCGAACC CGTCCTCGCC TGCCTGCCCG ACATCGGCAC GGTAGTGGCG CGTTCCATTG CCCACTTCTT CGCCCAAGCC GAACAGCAGG CGATGATAGA CGAGCTGCTC GCCGCAGGCG TTGCCCCGCA AGCCCAAGCC GTCAGCCTGC CTGCCGCACA ATATGCCGGG CCGCAACGCT GGATTACCCG CCTGCCCGGT TTCAAAATCA GCGAAAACAA AGCCCAAGCC TTATGGGAAC TCGCCGGACA AAGTATAGAA GGACTGCAAA ACGACAAAGC CCTCCCTGCC GACTGGCAGG CGTGGCGCAG CAAAGCGCAA AACACCGCCC TGCTCGAAAA CCTAAAAACC TTCTTCGCGC AAATGCCGTC TGAAGACGAG GCGGCGCAAG GTTCAGACGG CATCAATAAA GCCGTGGCAG GCAAAACCTT TGTGTTAACC GGCACCCTGC CCACCTTCAA ACGCGACCAA GCCCAAGCCC TAATCGAAGC CGCAGGCGGC AAAGTGTCCG GCAGCGTGTC CAAAAAAACC GACTACGTCG TCGCCGGAGA AGCCGCGGGC AGCAAGCTGG AAAAAGCCAA TGCCTTGGGC GTTTCCGTCC TCAGCGAAGC CGAACTGCTT ACCCTGCTCT GCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT