SSGCID: Target Page

NegoA.17932.a

glutamyl-tRNA reductase

CENTER ID:	NegoA.17932.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17932.a.B1.GE40079	full length		1	415

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.1764
UniProt:	B4RNE5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQLTAVGLNH QTAPLSIREK LAFAAAALPE AVRNLARSNA ATEAVILSTC NRTELYCVGD SEEIIRWLAD YHSLPIEEIR PYLYTLDMQE TVRHAFRVAC GLDSMVLGEP QILGQIKDAV RAAQEQESMG AKLNALFQKT FSVAKEVRTD TAVGENSVSM ASASVKLAEQ IFPDIGDLNV LFIGAGEMIE LVATYFAAKN PRLMTVANRT LARAQELCDK LGVNAEPCLL SDLPAILHDY DVVVSSTASQ LPIVGKGMVE RALKQRQSMP LFMLDLAVPR DIEAEVGDLN DAYLYTVDDM VNIVQSGKEA RQKAAAAAET LVSEKVAEFV RQQQGRQSVP LIKALRDEGE KARKQVLENA MKQLAKGATA EEVLERLSVQ LTNKLLHSPT QTLNKAGEED KDLVHAVAQI YHLDK

NT Sequence

ATGCAACTTA CCGCTGTCGG ACTCAATCAT CAAACCGCAC CTTTAAGCAT ACGGGAAAAG CTGGCGTTTG CCGCCGCCGC CCTGCCAGAA GCCGTCCGCA ATCTTGCCCG AAGCAATGCG GCAACGGAGG CGGTAATCCT TTCTACCTGC AACCGCACCG AGCTTTACTG CGTCGGCGAT TCGGAAGAAA TCATCCGATG GCTTGCCGAT TACCACAGTT TGCCGATTGA AGAAATCCGT CCGTATCTGT ACACGCTGGA TATGCAGGAA ACCGTGCGCC ACGCCTTCCG CGTTGCCTGC GGCTTGGATT CGATGGTTTT GGGCGAGCCG CAGATTTTGG GGCAGATTAA AGATGCGGTG CGTGCGGCTC AAGAACAGGA AAGTATGGGG GCAAAACTCA ATGCCCTGTT CCAAAAAACC TTTTCCGTTG CTAAAGAAGT CCGTACCGAT ACCGCTGTCG GCGAAAATTC GGTTTCGATG GCTTCCGCGT CCGTCAAGTT GGCGGAACAG ATTTTTCCCG ACATCGGCGA TTTGAACGTA TTGTTTATCG GCGCAGGCGA AATGATTGAG CTGGTTGCCA CTTATTTTGC CGCCAAAAAT CCCCGGCTGA TGACGGTTGC CAACCGGACG CTGGCGCGTG CACAGGAGTT GTGCGACAAG CTCGGTGTTA ACGCCGAACC GTGCCTGCTG TCCGATCTGC CTGCCATTCT GCACGATTAC GACGTGGTGG TTTCTTCAAC GGCGAGCCAG CTTCCGATAG TCGGCAAAGG CATGGTCGAA CGCGCATTGA AACAGCGTCA GAGTATGCCG TTGTTCATGC TTGACTTGGC CGTGCCGCGC GATATTGAAG CGGAAGTCGG CGATTTGAAC GATGCGTATC TTTATACGGT GGACGATATG GTCAACATCG TCCAAAGCGG CAAGGAGGCA AGGCAGAAGG CCGCCGCCGC CGCCGAAACG CTGGTGTCCG AGAAGGTTGC CGAATTTGTC AGGCAGCAGC AGGGCAGGCA GAGCGTTCCG CTGATTAAGG CCTTGCGGGA CGAGGGCGAG AAAGCGCGCA AGCAGGTGTT GGAAAATGCG ATGAAACAGC TTGCCAAAGG CGCAACGGCG GAAGAGGTTT TGGAACGGCT GTCCGTCCAA CTGACCAACA AGCTGCTGCA TTCGCCAACT CAAACCTTGA ATAAGGCGGG GGAAGAAGAT AAAGATTTGG TTCATGCCGT CGCGCAGATT TATCATTTGG ACAAATAA

Details for NegoA.17932.a.B1.GE40079

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/25/2016
EXPECTED MW:	46kDa
OBSERVED MW:	67kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ LTAVGLNHQT APLSIREKLA FAAAALPEAV RNLARSNAAT EAVILSTCNR TELYCVGDSE EIIRWLADYH SLPIEEIRPY LYTLDMQETV RHAFRVACGL DSMVLGEPQI LGQIKDAVRA AQEQESMGAK LNALFQKTFS VAKEVRTDTA VGENSVSMAS ASVKLAEQIF PDIGDLNVLF IGAGEMIELV ATYFAAKNPR LMTVANRTLA RAQELCDKLG VNAEPCLLSD LPAILHDYDV VVSSTASQLP IVGKGMVERA LKQRQSMPLF MLDLAVPRDI EAEVGDLNDA YLYTVDDMVN IVQSGKEARQ KAAAAAETLV SEKVAEFVRQ QQGRQSVPLI KALRDEGEKA RKQVLENAMK QLAKGATAEE VLERLSVQLT NKLLHSPTQT LNKAGEEDKD LVHA

Validated NT Sequence

acggcatgaa ccaaatcttt atcttcttcc cccgccttat tcaaggtttg agttggcgaa tgcagcagct tgttggtcag ttggacggac agccgttcca aaacctcttc cgccgttgcg cctttggcaa gctgtttcat cgcattttcc aacacctgct tgcgcgcttt ctcgccctcg tcccgcaagg ccttaatcag cggaacgctc tgcctgccct gctgctgcct gacaaattcg gcaacctttt cggacaccag cgtttcggcg gcggcggcgg ccttctgcct tgcctccttg ccgctttgga cgatgttgac catatcgtcc accgtataaa gatacgcatc gttcaaatcg ccgacttccg cttcaatatc gcgcggcacg gccaagtcaa gcatgaacaa cggcatactc tgacgctgtt tcaatgcgcg ttcgaccatg cctttgccga ctatcggaag ctggctcgcc gttgaagaaa ccaccacgtc gtaatcgtgc agaatggcag gcagatcgga cagcaggcac ggttcggcgt taacaccgag cttgtcgcac aactcctgtg cacgcgccag cgtccggttg gcaaccgtca tcagccgggg atttttggcg gcaaaataag tggcaaccag ctcaatcatt tcgcctgcgc cgataaacaa tacgttcaaa tcgccgatgt cgggaaaaat ctgttccgcc aacttgacgg acgcggaagc catcgaaacc gaattttcgc cgacagcggt atcggtacgg acttctttag caacggaaaa ggttttttgg aacagggcat tgagttttgc ccccatactt tcctgttctt gagccgcacg caccgcatct ttaatctgcc ccaaaatctg cggctcgccc aaaaccatcg aatccaagcc gcaggcaacg cggaaggcgt ggcgcacggt ttcctgcata tccagcgtgt acagatacgg acggatttct tcaatcggca aactgtggta atcggcaagc catcggatga tttcttccga atcgccgacg cagtaaagct cggtgcggtt gcaggtagaa aggattaccg cctccgttgc cgcattgctt cgggcaagat tgcggacggc ttctggcagg gcggcggcgg caaacgccag cttttcccgt atgcttaaag gtgcggtttg atgattgagt ccgacagcgg taagttgcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac ttaccgctgt cggactcaat catcaaaccg cacctttaag catacgggaa aagctggcgt ttgccgccgc cgccctgcca gaagccgtcc gcaatcttgc ccgaagcaat gcggcaacgg aggcggtaat cctttctacc tgcaaccgca ccgagcttta ctgcgtcggc gattcggaag aaatcatccg atggcttgcc gattaccaca gtttgccgat tgaagaaatc cgtccgtatc tgtacacgct ggatatgcag gaaaccgtgc gccacgcctt ccgcgttgcc tgcggcttgg attcgatggt tttgggcgag ccgcagattt tggggcagat taaagatgcg gtgcgtgcgg ctcaagaaca ggaaagtatg ggggcaaaac tcaatgccct gttccaaaaa accttttccg ttgctaaaga agtccgtacc gataccgctg tcggcgaaaa ttcggtttcg atggcttccg cgtccgtcaa gttggcggaa cagatttttc ccgacatcgg cgatttgaac gtattgttta tcggcgcagg cgaaatgatt gagctggttg ccacttattt tgccgccaaa aatccccggc tgatgacggt tgccaaccgg acgctggcgc gtgcacagga gttgtgcgac aagctcggtg ttaacgccga accgtgcctg ctgtccgatc tgcctgccat tctgcacgat tacgacgtgg tggtttcttc aacggcgagc cagcttccga tagtcggcaa aggcatggtc gaacgcgcat tgaaacagcg tcagagtatg ccgttgttca tgcttgactt ggccgtgccg cgcgatattg aagcggaagt cggcgatttg aacgatgcgt atctttatac ggtggacgat atggtcaaca tcgtccaaag cggcaaggag gcaaggcaga aggccgccgc cgccgccgaa acgctggtgt ccgagaaggt tgccgaattt gtcaggcagc agcagggcag gcagagcgtt ccgctgatta aggccttgcg ggacgagggc gagaaagcgc gcaagcaggt gttggaaaat gcgatgaaac agcttgccaa aggcgcaacg gcggaagagg ttttggaacg gctgtccgtc caactgacca acaagctgct gcattcgcca actcaaacct tgaataaggc gggggaagaa gataaagatt tggttcatgc cgtcgcgcag atttatcatt tggacaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat