NegoA.17926.a

glucosamine-fructose-6-phosphate aminotransferase [isomerizing]

CENTER ID: NegoA.17926.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17926.a.B1.GE40078 full length 1 612
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17926.a.B1.PW37936 full length 1 612

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.2362
UniProt: B4RPL9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCGIVGAIRA HHNVVDFLTD GLKRLEYRGY DSSGIAVNTD GKIKRVRRVG RVQLMEDAAR EKGISGGIGI GHTRWATHGG VTEPNAHPHI SGGMIAVVHN GIIENFESER KRLEGLGYRF ESQTDTEVIA HSINHEYAQN GGRLFEAVQE AVKRFHGAYA IAVIAQDKPD ELVVARMGCP LLVALGDDET FIASDVSAVI AFTRRVAYLE DGDIALLASD GIKRLTDKNG LPAERKVKVS ELSPASLEPG PHSHFMQKEI HEQPRAIADT AEVFLDGGFI PENFGKNAKS VFESIRSVKI LACGTSYYAA LTAKYWLESI AKIPADVEIA SEYRYRSVIA DPDQLVITIS QSGETLDTME ALKYAKSLGH RHSLSVCNVM ESALPRESSL VLYTRAGAEI GVASTKAFTT QLVVLFGLAV TLAKVRGVVS GEDEARYTEE LRQLPGSVQH ALNLEPQIAA WAQQFAKKTS ALFLGRGIHY PIALEGALKL KEITYIHAEA YPAGELKHGP LALVDENMPV VVIAPNDSLL DKVKANMQEV GARGGELFVF ADLDSNFNAA EGVHVIRAPR HVGELSPVVH TVPVQLLAYH TALARGTDVD KPRNLAKSVT VE
NT Sequence
ATGTGCGGTA TCGTCGGCGC CATCCGCGCC CATCACAACG TCGTCGATTT TCTGACCGAC GGCCTCAAAC GCCTCGAATA CCGGGGTTAT GACTCATCAG GCATTGCCGT CAACACCGAC GGCAAAATCA AACGCGTGCG CCGCGTCGGA CGCGTGCAGC TTATGGAGGA CGCGGCACGC GAAAAAGGCA TCAGCGGCGG CATCGGCATC GGGCATACGC GCTGGGCGAC ACACGGCGGC GTTACCGAGC CGAACGCCCA CCCGCACATC AGCGGCGGTA TGATTGCCGT CGTCCACAAC GGCATCATCG AAAACTTTGA AAGCGAACGC AAACGCTTGG AAGGTTTGGG ATACCGGTTT GAATCGCAAA CGGATACCGA AGTCATCGCA CACAGCATCA ACCACGAATA CGCGCAAAAC GGCGGCAGGC TGTTTGAAGC CGTGCAGGAA GCGGTCAAAC GTTTCCACGG CGCATACGCC ATCGCGGTTA TTGCCCAAGA CAAGCCTGAT GAATTAGTTG TGGCGCGTAT GGGCTGCCCG CTTTTGGTCG CTTTGGGCGA CGATGAAACC TTTATCGCTT CGGACGTATC CGCCGTCATC GCCTTTACGC GCCGCGTGGC GTACCTCGAA GACGGCGACA TCGCGCTGCT GGCTTCAGAC GGCATCAAAC GGCTGACCGA TAAAAACGGC CTGCCTGCCG AACGCAAAGT CAAGGTATCC GAACTCTCGC CCGCCTCTTT GGAACCGGGT CCGCACAGCC ACTTTATGCA AAAAGAAATC CACGAGCAGC CGCGCGCGAT TGCGGACACG GCGGAAGTTT TCCTCGACGG CGGATTCATC CCCGAAAACT TCGGCAAAAA TGCCAAAAGC GTGTTTGAAA GCATCCGCAG CGTCAAAATC CTTGCCTGCG GCACGTCCTA TTACGCCGCG CTGACCGCCA AATATTGGTT GGAATCCATC GCCAAAATCC CGGCCGACGT CGAAATCGCA AGCGAATACC GGTACCGCAG CGTGATTGCC GACCCCGACC AACTGGTCAT TACCATTTCC CAATCCGGCG AAACGCTGGA CACGATGGAG GCTTTGAAAT ACGCCAAATC TTTGGGACAC CGCCACAGCC TCTCCGTCTG CAACGTGATG GAATCCGCCC TGCCGCGCGA AAGCAGCCTT GTGCTTTATA CCCGTGCCGG TGCGGAAATC GGCGTCGCCT CGACTAAAGC ATTTACCACG CAACTGGTCG TGCTGTTCGG TTTGGCGGTA ACGCTGGCGA AAGTGCGCGG CGTGGTATCC GGCGAAGATG AAGCACGTTA CACCGAAGAA CTCCGCCAGC TTCCCGGCAG CGTGCAGCAC GCTTTGAACC TCGAACCGCA AATTGCCGCT TGGGCGCAAC AGTTTGCCAA GAAAACCAGC GCGCTGTTTT TGGGGCGCGG CATCCATTAC CCGATTGCCC TTGAAGGTGC GTTGAAGCTA AAGGAAATCA CCTACATCCA CGCCGAAGCC TATCCTGCCG GCGAACTGAA ACACGGGCCG CTTGCCTTGG TGGACGAAAA CATGCCCGTC GTCGTCATCG CGCCCAACGA CAGCCTGTTG GACAAAGTCA AAGCCAATAT GCAGGAAGTC GGCGCGCGCG GCGGCGAACT TTTCGTCTTT GCCGACCTCG ACAGCAATTT TAATGCCGCC GAAGGCGTGC ACGTTATCCG CGCACCTCGC CACGTCGGCG AACTGTCCCC CGTCGTGCAT ACCGTCCCCG TGCAGCTCCT CGCCTACCAC ACCGCCCTCG CGCGCGGCAC GGATGTGGAC AAGCCGCGCA ACCTGGCGAA ATCCGTAACC GTCGAATAA
Details for NegoA.17926.a.B1.GE40078
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 67kDa
OBSERVED MW: 67kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMC GIVGAIRAHH NVVDFLTDGL KRLEYRGYDS SGIAVYSDGK IKRVRRVGRV QLMEDAAREK GISGGIGIGH TRWATHGGVT EPNAHPHISG GMIAVVHNGI IENFESERKR LEGLGYRFES QTDTEVIAHS INHEYAQNGG RLFEAVQEAV KRFHGAYAIA VIAQDKPDEL VVARMGCPLL VALGDDETFI ASDVSAVIAF TRRVAYLEDG DIALLASDGI KRLTDKNGLP AERKVKVSEL SPASLEPGPH SHFMQKEIHE QPRAIADTXX XFLDGGFIPE TSAKCQSVFE SXXSVKIXAC GTSYYAALXA KYWLESIAKI PADVEIASEY RYRSVIADPD QLVITISQSG ETLDTMEALK YAKSLGHRHS LSVCNVMESA LPRESSLVLY TRAGAEIGVA STKAFTTQLV VLFGLAVTLA KVRGVVSGED EARYTEELRQ LPGSVQHALN LEPQIAAWAQ QFAKKTSALF LGRGIHYPIA LEGALKLKEI TYIHAEAYPA GELKHGPLAL VDENMPVVVI APNDSLLDKV KANMQEVGAR GGELFVFADL DSNFNATEGV HVIRAPRHVG ELSPVVHTVP VQLLAYHTAL ARGTDVDKPR NLAKSVTVE
Validated NT Sequence
atggctcacc accaccacca ccatatgtgc ggtatcgtcg gcgccatccg cgcccatcac aacgtcgtcg attttctgac cgacggcctc aaacgcctcg aataccgggg ttatgactca tcaggcattg ctgtgtattc agacggcaaa atcaaacgcg tgcgccgcgt cggacgcgtg cagcttatgg aggacgcggc acgcgaaaaa ggcatcagcg gcggcatcgg catcgggcat acgcgctggg cgacacacgg cggcgttacc gagccgaacg cccacccgca catcagcggc ggtatgattg ccgtcgtcca caacggcatc atcgaaaact ttgaaagcga acgcaaacgc ttggaaggtt tgggataccg gtttgaatcg caaacggata ccgaagtcat cgcacacagc atcaaccacg aatacgcgca aaacggcggc aggctgtttg aagccgtgca ggaagcggtc aaacgtttcc acggcgcata cgccatcgcg gttattgccc aagacaagcc tgatgaatta gttgtggcgc gtatgggctg cccgcttttg gtcgctttgg gcgacgatga aacctttatc gcttcggacg tatccgccgt catcgccttt acgcgccgcg tggcgtacct cgaagacggc gacatcgcgc tgctggcttc agacggcatc aaacggctga ccgataaaaa cggcctgcct gccgaacgca aagtcaaggt atccgaactc tcgcccgcct ctttggaacc gggtccgcac agccacttta tgcaaaaaga aatccacgag cagccgcgcg cgattgcgga cacggnggna nntttcctcg acggcggatt catccccgaa a-cttcggca aaa-tgccaa a-gcgtgttt gaaagcatnn gcagcgtcaa aatccnngcc tgcggcacgt cctattacgc cgcgctgncc gccaaatatt ggttggaatc catcgccaaa atcccggcng acgtcgaaat cgcaagcgaa taccggtacc gcagcgtgat tgccgacccc gaccaactgg tcattaccat ttcccaatcc ggcgaaacgc tggacacgat ggaggctttg aaatacgcca aatctttggg acaccgccac agcctctccg tctgcaacgt gatggaatcc gccctgccgc gcgaaagcag ccttgtgctt tatacccgtg ccggtgcgga aatcggcgtc gcctcgacta aagcatttac cacgcaactg gtcgtgctgt tcggtttggc ggtaacgctg gcgaaagtgc gcggcgtggt atccggcgaa gatgaagcac gttacaccga agaactccgc cagcttcccg gcagcgtgca gcacgctttg aacctcgaac cgcaaattgc cgcttgggcg caacagtttg ccaagaaaac cagcgcgctg tttttggggc gcggcatcca ttacccgatt gcccttgaag gtgcgttgaa gctaaaggaa atcacctaca tccacgccga agcctatcct gccggcgaac tgaaacacgg gccgcttgcc ttggtggacg aaaacatgcc cgtcgtcgtc atcgcgccca acgacagcct gttggacaaa gtcaaagcca atatgcagga agtcggcgcg cgcggcggcg aacttttcgt ctttgccgac ctcgacagca attttaatgc caccgaaggc gtacacgtta tccgcgcacc tcgccacgtc ggcgaactgt cccccgtcgt gcataccgtc cccgtgcagc tcctcgccta ccacaccgcc ctcgcgcgcg gcacggatgt ggacaagccg cgcaacctgg cgaaatccgt aaccgtcgaa taa
Expected Protein Sequence
MAHHHHHHMC GIVGAIRAHH NVVDFLTDGL KRLEYRGYDS SGIAVNTDGK IKRVRRVGRV QLMEDAAREK GISGGIGIGH TRWATHGGVT EPNAHPHISG GMIAVVHNGI IENFESERKR LEGLGYRFES QTDTEVIAHS INHEYAQNGG RLFEAVQEAV KRFHGAYAIA VIAQDKPDEL VVARMGCPLL VALGDDETFI ASDVSAVIAF TRRVAYLEDG DIALLASDGI KRLTDKNGLP AERKVKVSEL SPASLEPGPH SHFMQKEIHE QPRAIADTAE VFLDGGFIPE NFGKNAKSVF ESIRSVKILA CGTSYYAALT AKYWLESIAK IPADVEIASE YRYRSVIADP DQLVITISQS GETLDTMEAL KYAKSLGHRH SLSVCNVMES ALPRESSLVL YTRAGAEIGV ASTKAFTTQL VVLFGLAVTL AKVRGVVSGE DEARYTEELR QLPGSVQHAL NLEPQIAAWA QQFAKKTSAL FLGRGIHYPI ALEGALKLKE ITYIHAEAYP AGELKHGPLA LVDENMPVVV IAPNDSLLDK VKANMQEVGA RGGELFVFAD LDSNFNAAEG VHVIRAPRHV GELSPVVHTV PVQLLAYHTA LARGTDVDKP RNLAKSVTVE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtgcg gtatcgtcgg cgccatccgc gcccatcaca acgtcgtcga ttttctgacc gacggcctca aacgcctcga ataccggggt tatgactcat caggcattgc cgtcaacacc gacggcaaaa tcaaacgcgt gcgccgcgtc ggacgcgtgc agcttatgga ggacgcggca cgcgaaaaag gcatcagcgg cggcatcggc atcgggcata cgcgctgggc gacacacggc ggcgttaccg agccgaacgc ccacccgcac atcagcggcg gtatgattgc cgtcgtccac aacggcatca tcgaaaactt tgaaagcgaa cgcaaacgct tggaaggttt gggataccgg tttgaatcgc aaacggatac cgaagtcatc gcacacagca tcaaccacga atacgcgcaa aacggcggca ggctgtttga agccgtgcag gaagcggtca aacgtttcca cggcgcatac gccatcgcgg ttattgccca agacaagcct gatgaattag ttgtggcgcg tatgggctgc ccgcttttgg tcgctttggg cgacgatgaa acctttatcg cttcggacgt atccgccgtc atcgccttta cgcgccgcgt ggcgtacctc gaagacggcg acatcgcgct gctggcttca gacggcatca aacggctgac cgataaaaac ggcctgcctg ccgaacgcaa agtcaaggta tccgaactct cgcccgcctc tttggaaccg ggtccgcaca gccactttat gcaaaaagaa atccacgagc agccgcgcgc gattgcggac acggcggaag ttttcctcga cggcggattc atccccgaaa acttcggcaa aaatgccaaa agcgtgtttg aaagcatccg cagcgtcaaa atccttgcct gcggcacgtc ctattacgcc gcgctgaccg ccaaatattg gttggaatcc atcgccaaaa tcccggccga cgtcgaaatc gcaagcgaat accggtaccg cagcgtgatt gccgaccccg accaactggt cattaccatt tcccaatccg gcgaaacgct ggacacgatg gaggctttga aatacgccaa atctttggga caccgccaca gcctctccgt ctgcaacgtg atggaatccg ccctgccgcg cgaaagcagc cttgtgcttt atacccgtgc cggtgcggaa atcggcgtcg cctcgactaa agcatttacc acgcaactgg tcgtgctgtt cggtttggcg gtaacgctgg cgaaagtgcg cggcgtggta tccggcgaag atgaagcacg ttacaccgaa gaactccgcc agcttcccgg cagcgtgcag cacgctttga acctcgaacc gcaaattgcc gcttgggcgc aacagtttgc caagaaaacc agcgcgctgt ttttggggcg cggcatccat tacccgattg cccttgaagg tgcgttgaag ctaaaggaaa tcacctacat ccacgccgaa gcctatcctg ccggcgaact gaaacacggg ccgcttgcct tggtggacga aaacatgccc gtcgtcgtca tcgcgcccaa cgacagcctg ttggacaaag tcaaagccaa tatgcaggaa gtcggcgcgc gcggcggcga acttttcgtc tttgccgacc tcgacagcaa ttttaatgcc gccgaaggcg tgcacgttat ccgcgcacct cgccacgtcg gcgaactgtc ccccgtcgtg cataccgtcc ccgtgcagct cctcgcctac cacaccgccc tcgcgcgcgg cacggatgtg gacaagccgc gcaacctggc gaaatccgta accgtcgaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for NegoA.17926.a.B1.PW37936
PURIFICATION DATe: 8/8/2016
CONCENTRATION: 28.5mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMC GIVGAIRAHH NVVDFLTDGL KRLEYRGYDS SGIAVYSDGK IKRVRRVGRV QLMEDAAREK GISGGIGIGH TRWATHGGVT EPNAHPHISG GMIAVVHNGI IENFESERKR LEGLGYRFES QTDTEVIAHS INHEYAQNGG RLFEAVQEAV KRFHGAYAIA VIAQDKPDEL VVARMGCPLL VALGDDETFI ASDVSAVIAF TRRVAYLEDG DIALLASDGI KRLTDKNGLP AERKVKVSEL SPASLEPGPH SHFMQKEIHE QPRAIADTXX XFLDGGFIPE TSAKCQSVFE SXXSVKIXAC GTSYYAALXA KYWLESIAKI PADVEIASEY RYRSVIADPD QLVITISQSG ETLDTMEALK YAKSLGHRHS LSVCNVMESA LPRESSLVLY TRAGAEIGVA STKAFTTQLV VLFGLAVTLA KVRGVVSGED EARYTEELRQ LPGSVQHALN LEPQIAAWAQ QFAKKTSALF LGRGIHYPIA LEGALKLKEI TYIHAEAYPA GELKHGPLAL VDENMPVVVI APNDSLLDKV KANMQEVGAR GGELFVFADL DSNFNATEGV HVIRAPRHVG ELSPVVHTVP VQLLAYHTAL ARGTDVDKPR NLAKSVTVE
Validated NT Sequence
atggctcacc accaccacca ccatatgtgc ggtatcgtcg gcgccatccg cgcccatcac aacgtcgtcg attttctgac cgacggcctc aaacgcctcg aataccgggg ttatgactca tcaggcattg ctgtgtattc agacggcaaa atcaaacgcg tgcgccgcgt cggacgcgtg cagcttatgg aggacgcggc acgcgaaaaa ggcatcagcg gcggcatcgg catcgggcat acgcgctggg cgacacacgg cggcgttacc gagccgaacg cccacccgca catcagcggc ggtatgattg ccgtcgtcca caacggcatc atcgaaaact ttgaaagcga acgcaaacgc ttggaaggtt tgggataccg gtttgaatcg caaacggata ccgaagtcat cgcacacagc atcaaccacg aatacgcgca aaacggcggc aggctgtttg aagccgtgca ggaagcggtc aaacgtttcc acggcgcata cgccatcgcg gttattgccc aagacaagcc tgatgaatta gttgtggcgc gtatgggctg cccgcttttg gtcgctttgg gcgacgatga aacctttatc gcttcggacg tatccgccgt catcgccttt acgcgccgcg tggcgtacct cgaagacggc gacatcgcgc tgctggcttc agacggcatc aaacggctga ccgataaaaa cggcctgcct gccgaacgca aagtcaaggt atccgaactc tcgcccgcct ctttggaacc gggtccgcac agccacttta tgcaaaaaga aatccacgag cagccgcgcg cgattgcgga cacggnggna nntttcctcg acggcggatt catccccgaa a-cttcggca aaa-tgccaa a-gcgtgttt gaaagcatnn gcagcgtcaa aatccnngcc tgcggcacgt cctattacgc cgcgctgncc gccaaatatt ggttggaatc catcgccaaa atcccggcng acgtcgaaat cgcaagcgaa taccggtacc gcagcgtgat tgccgacccc gaccaactgg tcattaccat ttcccaatcc ggcgaaacgc tggacacgat ggaggctttg aaatacgcca aatctttggg acaccgccac agcctctccg tctgcaacgt gatggaatcc gccctgccgc gcgaaagcag ccttgtgctt tatacccgtg ccggtgcgga aatcggcgtc gcctcgacta aagcatttac cacgcaactg gtcgtgctgt tcggtttggc ggtaacgctg gcgaaagtgc gcggcgtggt atccggcgaa gatgaagcac gttacaccga agaactccgc cagcttcccg gcagcgtgca gcacgctttg aacctcgaac cgcaaattgc cgcttgggcg caacagtttg ccaagaaaac cagcgcgctg tttttggggc gcggcatcca ttacccgatt gcccttgaag gtgcgttgaa gctaaaggaa atcacctaca tccacgccga agcctatcct gccggcgaac tgaaacacgg gccgcttgcc ttggtggacg aaaacatgcc cgtcgtcgtc atcgcgccca acgacagcct gttggacaaa gtcaaagcca atatgcagga agtcggcgcg cgcggcggcg aacttttcgt ctttgccgac ctcgacagca attttaatgc caccgaaggc gtacacgtta tccgcgcacc tcgccacgtc ggcgaactgt cccccgtcgt gcataccgtc cccgtgcagc tcctcgccta ccacaccgcc ctcgcgcgcg gcacggatgt ggacaagccg cgcaacctgg cgaaatccgt aaccgtcgaa taa
Expressed Protein Sequence
MAHHHHHHMC GIVGAIRAHH NVVDFLTDGL KRLEYRGYDS SGIAVNTDGK IKRVRRVGRV QLMEDAAREK GISGGIGIGH TRWATHGGVT EPNAHPHISG GMIAVVHNGI IENFESERKR LEGLGYRFES QTDTEVIAHS INHEYAQNGG RLFEAVQEAV KRFHGAYAIA VIAQDKPDEL VVARMGCPLL VALGDDETFI ASDVSAVIAF TRRVAYLEDG DIALLASDGI KRLTDKNGLP AERKVKVSEL SPASLEPGPH SHFMQKEIHE QPRAIADTAE VFLDGGFIPE NFGKNAKSVF ESIRSVKILA CGTSYYAALT AKYWLESIAK IPADVEIASE YRYRSVIADP DQLVITISQS GETLDTMEAL KYAKSLGHRH SLSVCNVMES ALPRESSLVL YTRAGAEIGV ASTKAFTTQL VVLFGLAVTL AKVRGVVSGE DEARYTEELR QLPGSVQHAL NLEPQIAAWA QQFAKKTSAL FLGRGIHYPI ALEGALKLKE ITYIHAEAYP AGELKHGPLA LVDENMPVVV IAPNDSLLDK VKANMQEVGA RGGELFVFAD LDSNFNAAEG VHVIRAPRHV GELSPVVHTV PVQLLAYHTA LARGTDVDKP RNLAKSVTVE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTGCG GTATCGTCGG CGCCATCCGC GCCCATCACA ACGTCGTCGA TTTTCTGACC GACGGCCTCA AACGCCTCGA ATACCGGGGT TATGACTCAT CAGGCATTGC CGTCAACACC GACGGCAAAA TCAAACGCGT GCGCCGCGTC GGACGCGTGC AGCTTATGGA GGACGCGGCA CGCGAAAAAG GCATCAGCGG CGGCATCGGC ATCGGGCATA CGCGCTGGGC GACACACGGC GGCGTTACCG AGCCGAACGC CCACCCGCAC ATCAGCGGCG GTATGATTGC CGTCGTCCAC AACGGCATCA TCGAAAACTT TGAAAGCGAA CGCAAACGCT TGGAAGGTTT GGGATACCGG TTTGAATCGC AAACGGATAC CGAAGTCATC GCACACAGCA TCAACCACGA ATACGCGCAA AACGGCGGCA GGCTGTTTGA AGCCGTGCAG GAAGCGGTCA AACGTTTCCA CGGCGCATAC GCCATCGCGG TTATTGCCCA AGACAAGCCT GATGAATTAG TTGTGGCGCG TATGGGCTGC CCGCTTTTGG TCGCTTTGGG CGACGATGAA ACCTTTATCG CTTCGGACGT ATCCGCCGTC ATCGCCTTTA CGCGCCGCGT GGCGTACCTC GAAGACGGCG ACATCGCGCT GCTGGCTTCA GACGGCATCA AACGGCTGAC CGATAAAAAC GGCCTGCCTG CCGAACGCAA AGTCAAGGTA TCCGAACTCT CGCCCGCCTC TTTGGAACCG GGTCCGCACA GCCACTTTAT GCAAAAAGAA ATCCACGAGC AGCCGCGCGC GATTGCGGAC ACGGCGGAAG TTTTCCTCGA CGGCGGATTC ATCCCCGAAA ACTTCGGCAA AAATGCCAAA AGCGTGTTTG AAAGCATCCG CAGCGTCAAA ATCCTTGCCT GCGGCACGTC CTATTACGCC GCGCTGACCG CCAAATATTG GTTGGAATCC ATCGCCAAAA TCCCGGCCGA CGTCGAAATC GCAAGCGAAT ACCGGTACCG CAGCGTGATT GCCGACCCCG ACCAACTGGT CATTACCATT TCCCAATCCG GCGAAACGCT GGACACGATG GAGGCTTTGA AATACGCCAA ATCTTTGGGA CACCGCCACA GCCTCTCCGT CTGCAACGTG ATGGAATCCG CCCTGCCGCG CGAAAGCAGC CTTGTGCTTT ATACCCGTGC CGGTGCGGAA ATCGGCGTCG CCTCGACTAA AGCATTTACC ACGCAACTGG TCGTGCTGTT CGGTTTGGCG GTAACGCTGG CGAAAGTGCG CGGCGTGGTA TCCGGCGAAG ATGAAGCACG TTACACCGAA GAACTCCGCC AGCTTCCCGG CAGCGTGCAG CACGCTTTGA ACCTCGAACC GCAAATTGCC GCTTGGGCGC AACAGTTTGC CAAGAAAACC AGCGCGCTGT TTTTGGGGCG CGGCATCCAT TACCCGATTG CCCTTGAAGG TGCGTTGAAG CTAAAGGAAA TCACCTACAT CCACGCCGAA GCCTATCCTG CCGGCGAACT GAAACACGGG CCGCTTGCCT TGGTGGACGA AAACATGCCC GTCGTCGTCA TCGCGCCCAA CGACAGCCTG TTGGACAAAG TCAAAGCCAA TATGCAGGAA GTCGGCGCGC GCGGCGGCGA ACTTTTCGTC TTTGCCGACC TCGACAGCAA TTTTAATGCC GCCGAAGGCG TGCACGTTAT CCGCGCACCT CGCCACGTCG GCGAACTGTC CCCCGTCGTG CATACCGTCC CCGTGCAGCT CCTCGCCTAC CACACCGCCC TCGCGCGCGG CACGGATGTG GACAAGCCGC GCAACCTGGC GAAATCCGTA ACCGTCGAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT