NegoA.17901.b

peptide chain release factor 1

CENTER ID:	NegoA.17901.b
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17901.b.B1.GE40075	full length		1	358

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17901.b.B1.PS37971	full length		1	358

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_85047

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.1666
UniProt:	B4RN52

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPSILEKLQ QLGDRLEEVT HLLGQPEATS DMDNYRKLTR EHAELTPVVE VFQNYRLAQS DLADAEEMLS DPEMKDFAAE EIEAAKAKID ELDTELQKLL LPKDADDDKN IFIEIRAGTG GGEAALFAGD LLRMYSRYAE RNRWQVEIVS ANESELGGYK EVIARIVGLG AYSRLKFESG GHRVQRVPAT ESQGRIHTSA CTVAVMPEAD ELEDIELNPA DLRTDTFRAS GAGGQHINKT DSAVRITHLP TGMVVECQDG RSQHANKAQA MKVLAARLND AQKREVQAKE AAERKSLIGS GDRSERIRTY NYPQGRVTDH RINLTLHKLD FVMDGDLAEI TDALIAEHQA ELLAAMGD

NT Sequence

ATGAAGCCGT CTATCTTAGA AAAACTGCAA CAACTCGGCG ACCGGTTGGA AGAAGTTACC CACCTTTTAG GGCAACCCGA AGCCACATCC GATATGGACA ACTACCGCAA GCTCACGCGC GAACACGCCG AACTGACCCC CGTCGTCGAA GTATTCCAAA ACTATCGGCT TGCCCAAAGC GACTTGGCGG ATGCCGAAGA AATGTTGTCC GACCCCGAAA TGAAAGACTT TGCCGCCGAA GAAATCGAAG CGGCGAAAGC CAAAATCGAC GAACTCGATA CCGAATTGCA AAAACTGCTG CTGCCCAAAG ATGCCGACGA CGACAAAAAC ATCTTTATCG AAATACGCGC CGGCACGGGC GGCGGCGAAG CCGCGCTGTT TGCCGGCGAT TTGCTGCGTA TGTACAGCCG CTACGCCGAA CGCAACCGCT GGCAGGTTGA AATCGTGTCC GCCAACGAAA GCGAATTGGG CGGCTATAAA GAAGTCATCG CCCGTATTGT CGGACTCGGC GCGTACAGCC GTCTGAAATT TGAATCGGGC GGACACCGCG TGCAGCGCGT CCCCGCCACC GAAAGCCAAG GCCGCATCCA CACCTCCGCC TGTACCGTTG CCGTCATGCC CGAAGCGGAC GAGCTCGAAG ACATCGAACT GAACCCCGCC GACCTGCGCA CCGATACCTT CCGCGCATCC GGCGCGGGCG GTCAGCACAT TAACAAAACC GACTCCGCCG TCCGCATTAC CCACTTGCCG ACAGGCATGG TGGTCGAATG CCAAGACGGC CGCAGCCAAC ACGCCAACAA AGCGCAGGCG ATGAAAGTCC TCGCCGCCCG CCTGAACGAC GCGCAAAAAC GCGAAGTCCA AGCCAAAGAA GCCGCCGAAC GCAAATCCCT CATCGGCAGC GGCGACCGCA GCGAACGCAT CCGCACCTAC AACTATCCCC AAGGCAGGGT AACAGACCAC CGCATCAACC TCACCCTGCA CAAGCTGGAT TTTGTGATGG ACGGCGATTT GGCAGAAATC ACCGATGCCC TGATTGCCGA ACATCAGGCG GAGCTTCTGG CGGCGATGGG CGATTGA

Details for NegoA.17901.b.B1.GE40075

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	41kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMK PSILEKLQQL GDRLEEVTHL LGQPEATSDM DNYRKLTREH AELTPVVEVF QNYRLAQSDL ADAEEMLSDP EMKDFAAEEI EAAKAKIDEL DTELQKLLLP KDADDDKNIF IEIRAGTGGD EAALFAGDLL RMYSRYAERN RWQVEIVSAN ESELGGYKEV IARIVGLGAY SRLKFESGGH RVQRVPATES QGRIHTSACT VAVMPEADEL EDIELNPADL RTDTFRASGA GGQHINKTDS AVRITHLPTG MVVECQDGRS QHANKAQAMK VLAARLNDAQ KREVQAKEAA ERKSLIGSGD RSERIRTYNY PQGRVTDHRI NLTLHKLDFV MDGDLAEITD ALIAEHQA

Validated NT Sequence

ctcngcctga tgttcggcaa tcagggcatc ggtgatttct gccaaatcgc cgtccatcac aaaatccagc ttgtgcaggg tgaggttgat gcggtggtct gttaccctgc cttggggata gttgtaggtg cggatgcgtt cgctgcggtc gccgctgccg atgagggatt tgcgttcggc ggcttctttg gcttggactt cgcgtttttg cgcgtcgttc aggcgggcgg cgaggacttt catcgcctgc gctttgttgg cgtgttggct gcggccgtct tggcattcga ccaccatgcc tgtcggcaag tgggtaatgc ggacggcgga gtcggttttg ttaatgtgct gaccgcccgc gccggatgcg cggaaggtat cggtgcgcag gtcggcgggg ttcagttcga tgtcttcgag ctcgtccgct tcgggcatga cggcaacggt acaggcggag gtgtggatgc ggccttggct ttcggtggcg gggacgcgct gcacgcggtg tccgcccgat tcaaatttca gacggctgta cgcgccgagt ccgacaatac gggcgatgac ttctttatag ccgcccaatt cgctttcgtt ggcggacacg atttcaacct gccagcggtt gcgttcggcg tagcggctgt acatacgcag caaatcgccg gcaaacagcg cggcttcgtc gccgcccgtg ccggcgcgta tttcgataaa gatgtttttg tcgtcgtcgg catctttggg cagcagcagt ttttgcaatt cggtatccag ttcgtcgatt ttggctttcg ccgcttcgat ttcttcggcg gcaaagtctt tcatttcggg gtcggacaac atttcttcgg catccgccaa gtcgctttgg gcaagccgat agttttggaa tacttcgacg acgggggtca gttcggcgtg ttcgcgcgtg agcttgcggt agttgtccat atcggatgtg gcttcgggtt gccctaaaag gtgggtaact tcttccaacc ggtcgccgag ttgttgcagt ttttctaaga tagacggctt catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHMK PSILEKLQQL GDRLEEVTHL LGQPEATSDM DNYRKLTREH AELTPVVEVF QNYRLAQSDL ADAEEMLSDP EMKDFAAEEI EAAKAKIDEL DTELQKLLLP KDADDDKNIF IEIRAGTGGG EAALFAGDLL RMYSRYAERN RWQVEIVSAN ESELGGYKEV IARIVGLGAY SRLKFESGGH RVQRVPATES QGRIHTSACT VAVMPEADEL EDIELNPADL RTDTFRASGA GGQHINKTDS AVRITHLPTG MVVECQDGRS QHANKAQAMK VLAARLNDAQ KREVQAKEAA ERKSLIGSGD RSERIRTYNY PQGRVTDHRI NLTLHKLDFV MDGDLAEITD ALIAEHQAEL LAAMGD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagc cgtctatctt agaaaaactg caacaactcg gcgaccggtt ggaagaagtt acccaccttt tagggcaacc cgaagccaca tccgatatgg acaactaccg caagctcacg cgcgaacacg ccgaactgac ccccgtcgtc gaagtattcc aaaactatcg gcttgcccaa agcgacttgg cggatgccga agaaatgttg tccgaccccg aaatgaaaga ctttgccgcc gaagaaatcg aagcggcgaa agccaaaatc gacgaactcg ataccgaatt gcaaaaactg ctgctgccca aagatgccga cgacgacaaa aacatcttta tcgaaatacg cgccggcacg ggcggcggcg aagccgcgct gtttgccggc gatttgctgc gtatgtacag ccgctacgcc gaacgcaacc gctggcaggt tgaaatcgtg tccgccaacg aaagcgaatt gggcggctat aaagaagtca tcgcccgtat tgtcggactc ggcgcgtaca gccgtctgaa atttgaatcg ggcggacacc gcgtgcagcg cgtccccgcc accgaaagcc aaggccgcat ccacacctcc gcctgtaccg ttgccgtcat gcccgaagcg gacgagctcg aagacatcga actgaacccc gccgacctgc gcaccgatac cttccgcgca tccggcgcgg gcggtcagca cattaacaaa accgactccg ccgtccgcat tacccacttg ccgacaggca tggtggtcga atgccaagac ggccgcagcc aacacgccaa caaagcgcag gcgatgaaag tcctcgccgc ccgcctgaac gacgcgcaaa aacgcgaagt ccaagccaaa gaagccgccg aacgcaaatc cctcatcggc agcggcgacc gcagcgaacg catccgcacc tacaactatc cccaaggcag ggtaacagac caccgcatca acctcaccct gcacaagctg gattttgtga tggacggcga tttggcagaa atcaccgatg ccctgattgc cgaacatcag gcggagcttc tggcggcgat gggcgattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for NegoA.17901.b.B1.PS37971

PURIFICATION DATe:	7/6/2016
CONCENTRATION:	10.12mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGC CGTCTATCTT AGAAAAACTG CAACAACTCG GCGACCGGTT GGAAGAAGTT ACCCACCTTT TAGGGCAACC CGAAGCCACA TCCGATATGG ACAACTACCG CAAGCTCACG CGCGAACACG CCGAACTGAC CCCCGTCGTC GAAGTATTCC AAAACTATCG GCTTGCCCAA AGCGACTTGG CGGATGCCGA AGAAATGTTG TCCGACCCCG AAATGAAAGA CTTTGCCGCC GAAGAAATCG AAGCGGCGAA AGCCAAAATC GACGAACTCG ATACCGAATT GCAAAAACTG CTGCTGCCCA AAGATGCCGA CGACGACAAA AACATCTTTA TCGAAATACG CGCCGGCACG GGCGGCGGCG AAGCCGCGCT GTTTGCCGGC GATTTGCTGC GTATGTACAG CCGCTACGCC GAACGCAACC GCTGGCAGGT TGAAATCGTG TCCGCCAACG AAAGCGAATT GGGCGGCTAT AAAGAAGTCA TCGCCCGTAT TGTCGGACTC GGCGCGTACA GCCGTCTGAA ATTTGAATCG GGCGGACACC GCGTGCAGCG CGTCCCCGCC ACCGAAAGCC AAGGCCGCAT CCACACCTCC GCCTGTACCG TTGCCGTCAT GCCCGAAGCG GACGAGCTCG AAGACATCGA ACTGAACCCC GCCGACCTGC GCACCGATAC CTTCCGCGCA TCCGGCGCGG GCGGTCAGCA CATTAACAAA ACCGACTCCG CCGTCCGCAT TACCCACTTG CCGACAGGCA TGGTGGTCGA ATGCCAAGAC GGCCGCAGCC AACACGCCAA CAAAGCGCAG GCGATGAAAG TCCTCGCCGC CCGCCTGAAC GACGCGCAAA AACGCGAAGT CCAAGCCAAA GAAGCCGCCG AACGCAAATC CCTCATCGGC AGCGGCGACC GCAGCGAACG CATCCGCACC TACAACTATC CCCAAGGCAG GGTAACAGAC CACCGCATCA ACCTCACCCT GCACAAGCTG GATTTTGTGA TGGACGGCGA TTTGGCAGAA ATCACCGATG CCCTGATTGC CGAACATCAG GCGGAGCTTC TGGCGGCGAT GGGCGATTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT