NegoA.17899.a

FolC protein

CENTER ID: NegoA.17899.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17899.a.B1.GE40073 full length 1 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17899.a.B1.PS37965 full length 1 424

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.455
UniProt: B4RJU9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTLQDWLSH LETAHSGGLI DMGLERVSEV KKRMNLTPQC PVVVVAGTNG KGSVCAYLTQ IYKQAGFKTG TLTSPHLLHY NERIAINAEP VSDDTIIASF ERIEAARGEI SLTYFEFNAL AAVDIFIREQ VDVMILEVGL GGRLDAVNAF DGDCAVVTSV DLDHQAFLGD TVEQVGFEKA GVFRSGKPAI CGQNPAPASL VAHAEAIGAK LLMVQRDFEF HAMENIQWNY RFRPQHSDGP ARNRNALPFP ALRGAYQLSN AACALTVLEC LDDRLPVDIG AIKRGLLLVE NPGRFQVLPG RPLTVLDVGH NPHAARALRR NLINLAYAQK RTAVFSMLSD KDIDGVSETV KDQFDEWYIA PLDVPRGMTA DALKAKLEQH HIENIQTFAA VRDAYRAAAS KAGEDDRIVV FGSFHTVADV MSVL
NT Sequence
ATGAAAACAC TACAAGACTG GCTCTCGCAT TTGGAAACCG CCCACAGCGG CGGTTTGATC GACATGGGTT TGGAGCGCGT AAGCGAAGTA AAAAAGCGCA TGAACCTGAC ACCGCAATGC CCCGTCGTCG TCGTCGCGGG AACAAACGGC AAAGGTTCGG TCTGCGCCTA CCTGACACAG ATTTACAAAC AGGCAGGCTT CAAAACCGGC ACGCTGACCA GCCCGCATTT ATTGCATTAC AACGAACGCA TCGCCATCAA CGCCGAACCC GTTTCGGACG ATACGATTAT TGCCTCTTTC GAGCGCATCG AAGCCGCGCG CGGCGAAATA TCGCTGACTT ATTTTGAATT CAATGCCTTG GCGGCTGTCG ACATCTTCAT ACGCGAACAA GTGGATGTAA TGATATTGGA AGTCGGCTTG GGCGGACGTT TGGACGCGGT CAACGCGTTT GACGGCGATT GCGCGGTGGT TACTAGTGTG GATTTGGACC ATCAGGCATT TTTGGGCGAT ACGGTCGAAC AGGTCGGCTT TGAAAAGGCA GGCGTGTTCC GCAGCGGCAA ACCCGCCATC TGCGGTCAAA ACCCCGCGCC CGCATCATTG GTCGCACACG CCGAAGCCAT AGGCGCGAAA CTGCTGATGG TACAGCGCGA TTTCGAGTTT CACGCGATGG AAAACATCCA ATGGAACTAC CGCTTCCGGC CGCAGCATTC AGACGGTCCC GCACGCAACC GCAATGCCCT GCCCTTCCCC GCATTGCGCG GCGCATACCA GCTTTCTAAC GCCGCCTGCG CGTTGACCGT ATTGGAATGC CTCGACGACA GACTGCCGGT GGACATTGGT GCAATCAAAC GCGGGCTGCT GCTGGTTGAA AATCCCGGAC GCTTCCAAGT CCTGCCCGGC CGGCCGCTGA CCGTATTGGA TGTCGGACAC AACCCCCACG CCGCCCGCGC CCTGCGCCGC AACCTGATCA ACCTTGCCTA CGCGCAAAAG CGCACCGCCG TGTTCAGTAT GCTGTCCGAC AAAGACATAG ACGGCGTGTC GGAAACCGTT AAAGACCAGT TTGACGAGTG GTATATCGCG CCTTTGGACG TGCCGCGCGG TATGACGGCG GATGCACTGA AAGCCAAATT GGAACAACAC CACATCGAAA ACATACAGAC ATTCGCCGCC GTCCGCGACG CGTACCGGGC GGCAGCCTCA AAAGCGGGCG AAGACGACAG AATCGTCGTA TTCGGCTCAT TTCATACCGT CGCCGACGTA ATGTCGGTAC TGTGA
Details for NegoA.17899.a.B1.GE40073
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 47kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMK TLQDWLSHLE TAHSGGLIDM GLERVSEVKK RMNLTPQCPV VVVAGTNGKG SVCAYLTQIY KQAGFKTGTL TSPHLLHYNE RIAINAEPVS DDTIIASFER IEAARGEISL TYFEFNALAA VDIFIREQVD VMILEVGLGG RLDAVNAFDG DCAVVTSVDL DHQAFLGDTV EQVGFEKAGV FRSGKPAICG QNPAPASLVA HAEAIGAKLL MVQRDFEFHA MENIQWNYRF RPQHSDGPAR NRNALPFPAL RGAYQLSNAA CALTVLECLD DRLPVDIGAI KRGLLLVENP GRFQVLPGRP LTVLDVGHNP HAARALRRNL INLAYAQKRT AVFSMLSDKD IDGVSETVKD QFDEWYIAPL DVPRGMTADA LKAKLEQHHI ENIQTFAAVR DAYRAAASKA GEDDRIVVFG SFH
Validated NT Sequence
gtatgaaatg agccgaatac gacgattctg tcgtcttcgc ccgcttttga ggctgccgcc cggtacgcgt cgcggacggc ggcgaatgtc tgtatgtttt cgatgtggtg ttgttccaat ttggctttca gtgcatccgc cgtcataccg cgcggcacgt ccaaaggcgc gatataccac tcgtcaaact ggtctttaac ggtttccgac acgccgtcta tgtctttgtc ggacagcata ctgaacacgg cggtgcgctt ttgcgcgtag gcaaggttga ttaggttgcg gcgcagggcg cgggcggcgt gggggttgtg tccgacatcc aatacggtca gcggccggcc gggcaggact tggaagcgtc cgggattttc aaccagcagc agcccgcgtt tgattgcacc aatgtccacc ggcagtctgt cgtcgaggca ttccaatacg gtcaacgcgc aggcggcgtt agaaagctgg tatgcgccgc gcaatgcggg gaagggcagg gcattgcggt tgcgtgcggg gccgtctgaa tgctgcggcc ggaagcggta gttccattgg atgttttcca tcgcgtgaaa ctcgaaatcg cgctgtacca tcagcagttt cgcgcctatg gcttcggcgt gtgcgaccaa tgatgcgggc gcggggtttt gaccgcagat ggcgggtttg ccgctgcgga acacgcctgc cttttcaaag ccgacctgtt cgaccgtatc gcccaaaaat gcctgatggt ccaaatccac actagtaacc accgcgcaat cgccgtcaaa cgcgttgacc gcgtccaaac gtccgcccaa gccgacttcc aatatcatta catccacttg ttcgcgtatg aaaatgtcga cagccgccaa ggcattgaat tcaaaataag tcagcgatat ttcgccgcgc gcggcttcga tgcgctcgaa agaggcaata atcgtatcgt ccgaaacggg ttcggcgttg atggcgatgc gttcgttgta atgcaataaa tgcgggctgg tcagcgtgcc ggttttgaag cctgcctgtt tgtaaatctg tgtcaggtag gcgcagaccg aacctttgcc gtttgttccc gcgacgacga cgacggggca ttgcggtgtc aggttcatgc gcttttttac ttcgcttacg cgctccaaac ccatgtcgat caaaccgccg ctgtgggcgg tttccaaatg cgagagccag tcttgtagtg ttttcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMK TLQDWLSHLE TAHSGGLIDM GLERVSEVKK RMNLTPQCPV VVVAGTNGKG SVCAYLTQIY KQAGFKTGTL TSPHLLHYNE RIAINAEPVS DDTIIASFER IEAARGEISL TYFEFNALAA VDIFIREQVD VMILEVGLGG RLDAVNAFDG DCAVVTSVDL DHQAFLGDTV EQVGFEKAGV FRSGKPAICG QNPAPASLVA HAEAIGAKLL MVQRDFEFHA MENIQWNYRF RPQHSDGPAR NRNALPFPAL RGAYQLSNAA CALTVLECLD DRLPVDIGAI KRGLLLVENP GRFQVLPGRP LTVLDVGHNP HAARALRRNL INLAYAQKRT AVFSMLSDKD IDGVSETVKD QFDEWYIAPL DVPRGMTADA LKAKLEQHHI ENIQTFAAVR DAYRAAASKA GEDDRIVVFG SFHTVADVMS VL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa cactacaaga ctggctctcg catttggaaa ccgcccacag cggcggtttg atcgacatgg gtttggagcg cgtaagcgaa gtaaaaaagc gcatgaacct gacaccgcaa tgccccgtcg tcgtcgtcgc gggaacaaac ggcaaaggtt cggtctgcgc ctacctgaca cagatttaca aacaggcagg cttcaaaacc ggcacgctga ccagcccgca tttattgcat tacaacgaac gcatcgccat caacgccgaa cccgtttcgg acgatacgat tattgcctct ttcgagcgca tcgaagccgc gcgcggcgaa atatcgctga cttattttga attcaatgcc ttggcggctg tcgacatctt catacgcgaa caagtggatg taatgatatt ggaagtcggc ttgggcggac gtttggacgc ggtcaacgcg tttgacggcg attgcgcggt ggttactagt gtggatttgg accatcaggc atttttgggc gatacggtcg aacaggtcgg ctttgaaaag gcaggcgtgt tccgcagcgg caaacccgcc atctgcggtc aaaaccccgc gcccgcatca ttggtcgcac acgccgaagc cataggcgcg aaactgctga tggtacagcg cgatttcgag tttcacgcga tggaaaacat ccaatggaac taccgcttcc ggccgcagca ttcagacggt cccgcacgca accgcaatgc cctgcccttc cccgcattgc gcggcgcata ccagctttct aacgccgcct gcgcgttgac cgtattggaa tgcctcgacg acagactgcc ggtggacatt ggtgcaatca aacgcgggct gctgctggtt gaaaatcccg gacgcttcca agtcctgccc ggccggccgc tgaccgtatt ggatgtcgga cacaaccccc acgccgcccg cgccctgcgc cgcaacctga tcaaccttgc ctacgcgcaa aagcgcaccg ccgtgttcag tatgctgtcc gacaaagaca tagacggcgt gtcggaaacc gttaaagacc agtttgacga gtggtatatc gcgcctttgg acgtgccgcg cggtatgacg gcggatgcac tgaaagccaa attggaacaa caccacatcg aaaacataca gacattcgcc gccgtccgcg acgcgtaccg ggcggcagcc tcaaaagcgg gcgaagacga cagaatcgtc gtattcggct catttcatac cgtcgccgac gtaatgtcgg tactgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for NegoA.17899.a.B1.PS37965
PURIFICATION DATe: 6/30/2016
CONCENTRATION: 12.7mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK TLQDWLSHLE TAHSGGLIDM GLERVSEVKK RMNLTPQCPV VVVAGTNGKG SVCAYLTQIY KQAGFKTGTL TSPHLLHYNE RIAINAEPVS DDTIIASFER IEAARGEISL TYFEFNALAA VDIFIREQVD VMILEVGLGG RLDAVNAFDG DCAVVTSVDL DHQAFLGDTV EQVGFEKAGV FRSGKPAICG QNPAPASLVA HAEAIGAKLL MVQRDFEFHA MENIQWNYRF RPQHSDGPAR NRNALPFPAL RGAYQLSNAA CALTVLECLD DRLPVDIGAI KRGLLLVENP GRFQVLPGRP LTVLDVGHNP HAARALRRNL INLAYAQKRT AVFSMLSDKD IDGVSETVKD QFDEWYIAPL DVPRGMTADA LKAKLEQHHI ENIQTFAAVR DAYRAAASKA GEDDRIVVFG SFH
Validated NT Sequence
gtatgaaatg agccgaatac gacgattctg tcgtcttcgc ccgcttttga ggctgccgcc cggtacgcgt cgcggacggc ggcgaatgtc tgtatgtttt cgatgtggtg ttgttccaat ttggctttca gtgcatccgc cgtcataccg cgcggcacgt ccaaaggcgc gatataccac tcgtcaaact ggtctttaac ggtttccgac acgccgtcta tgtctttgtc ggacagcata ctgaacacgg cggtgcgctt ttgcgcgtag gcaaggttga ttaggttgcg gcgcagggcg cgggcggcgt gggggttgtg tccgacatcc aatacggtca gcggccggcc gggcaggact tggaagcgtc cgggattttc aaccagcagc agcccgcgtt tgattgcacc aatgtccacc ggcagtctgt cgtcgaggca ttccaatacg gtcaacgcgc aggcggcgtt agaaagctgg tatgcgccgc gcaatgcggg gaagggcagg gcattgcggt tgcgtgcggg gccgtctgaa tgctgcggcc ggaagcggta gttccattgg atgttttcca tcgcgtgaaa ctcgaaatcg cgctgtacca tcagcagttt cgcgcctatg gcttcggcgt gtgcgaccaa tgatgcgggc gcggggtttt gaccgcagat ggcgggtttg ccgctgcgga acacgcctgc cttttcaaag ccgacctgtt cgaccgtatc gcccaaaaat gcctgatggt ccaaatccac actagtaacc accgcgcaat cgccgtcaaa cgcgttgacc gcgtccaaac gtccgcccaa gccgacttcc aatatcatta catccacttg ttcgcgtatg aaaatgtcga cagccgccaa ggcattgaat tcaaaataag tcagcgatat ttcgccgcgc gcggcttcga tgcgctcgaa agaggcaata atcgtatcgt ccgaaacggg ttcggcgttg atggcgatgc gttcgttgta atgcaataaa tgcgggctgg tcagcgtgcc ggttttgaag cctgcctgtt tgtaaatctg tgtcaggtag gcgcagaccg aacctttgcc gtttgttccc gcgacgacga cgacggggca ttgcggtgtc aggttcatgc gcttttttac ttcgcttacg cgctccaaac ccatgtcgat caaaccgccg ctgtgggcgg tttccaaatg cgagagccag tcttgtagtg ttttcatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMK TLQDWLSHLE TAHSGGLIDM GLERVSEVKK RMNLTPQCPV VVVAGTNGKG SVCAYLTQIY KQAGFKTGTL TSPHLLHYNE RIAINAEPVS DDTIIASFER IEAARGEISL TYFEFNALAA VDIFIREQVD VMILEVGLGG RLDAVNAFDG DCAVVTSVDL DHQAFLGDTV EQVGFEKAGV FRSGKPAICG QNPAPASLVA HAEAIGAKLL MVQRDFEFHA MENIQWNYRF RPQHSDGPAR NRNALPFPAL RGAYQLSNAA CALTVLECLD DRLPVDIGAI KRGLLLVENP GRFQVLPGRP LTVLDVGHNP HAARALRRNL INLAYAQKRT AVFSMLSDKD IDGVSETVKD QFDEWYIAPL DVPRGMTADA LKAKLEQHHI ENIQTFAAVR DAYRAAASKA GEDDRIVVFG SFHTVADVMS VL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA CACTACAAGA CTGGCTCTCG CATTTGGAAA CCGCCCACAG CGGCGGTTTG ATCGACATGG GTTTGGAGCG CGTAAGCGAA GTAAAAAAGC GCATGAACCT GACACCGCAA TGCCCCGTCG TCGTCGTCGC GGGAACAAAC GGCAAAGGTT CGGTCTGCGC CTACCTGACA CAGATTTACA AACAGGCAGG CTTCAAAACC GGCACGCTGA CCAGCCCGCA TTTATTGCAT TACAACGAAC GCATCGCCAT CAACGCCGAA CCCGTTTCGG ACGATACGAT TATTGCCTCT TTCGAGCGCA TCGAAGCCGC GCGCGGCGAA ATATCGCTGA CTTATTTTGA ATTCAATGCC TTGGCGGCTG TCGACATCTT CATACGCGAA CAAGTGGATG TAATGATATT GGAAGTCGGC TTGGGCGGAC GTTTGGACGC GGTCAACGCG TTTGACGGCG ATTGCGCGGT GGTTACTAGT GTGGATTTGG ACCATCAGGC ATTTTTGGGC GATACGGTCG AACAGGTCGG CTTTGAAAAG GCAGGCGTGT TCCGCAGCGG CAAACCCGCC ATCTGCGGTC AAAACCCCGC GCCCGCATCA TTGGTCGCAC ACGCCGAAGC CATAGGCGCG AAACTGCTGA TGGTACAGCG CGATTTCGAG TTTCACGCGA TGGAAAACAT CCAATGGAAC TACCGCTTCC GGCCGCAGCA TTCAGACGGT CCCGCACGCA ACCGCAATGC CCTGCCCTTC CCCGCATTGC GCGGCGCATA CCAGCTTTCT AACGCCGCCT GCGCGTTGAC CGTATTGGAA TGCCTCGACG ACAGACTGCC GGTGGACATT GGTGCAATCA AACGCGGGCT GCTGCTGGTT GAAAATCCCG GACGCTTCCA AGTCCTGCCC GGCCGGCCGC TGACCGTATT GGATGTCGGA CACAACCCCC ACGCCGCCCG CGCCCTGCGC CGCAACCTGA TCAACCTTGC CTACGCGCAA AAGCGCACCG CCGTGTTCAG TATGCTGTCC GACAAAGACA TAGACGGCGT GTCGGAAACC GTTAAAGACC AGTTTGACGA GTGGTATATC GCGCCTTTGG ACGTGCCGCG CGGTATGACG GCGGATGCAC TGAAAGCCAA ATTGGAACAA CACCACATCG AAAACATACA GACATTCGCC GCCGTCCGCG ACGCGTACCG GGCGGCAGCC TCAAAAGCGG GCGAAGACGA CAGAATCGTC GTATTCGGCT CATTTCATAC CGTCGCCGAC GTAATGTCGG TACTGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT