NegoA.17885.a
aspartate-semialdehyde dehydrogenase
| CENTER ID: | NegoA.17885.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.17885.a.B1.GE40071 | full length | 1 | 371 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.17885.a.B1.PW37925 | full length | 1 | 371 |
Structures
6BAC
DEPOSITED: 10/12/2017
DETERMINATION: XRay
CLONE: NegoA.17885.a.B1.GE40071
PROTEIN: NegoA.17885.a.B1.PW37925
Publications by SSGCID
Structural Characterization of Aspartate-Semialdehyde Dehydrogenase from Pseudomonas aeruginosa and Neisseria gonorrhoeae
Abendroth J, Dranow DM, Edwards TE, Fairman JW, Lorimer DD, Muruthi MM, Forwood JK, Teakel S
Sci Rep - 2022
volume 12, issue 1, pages 14010
PMID: 35977963; PMCID: PMC9385607
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.2420 |
| UniProt: | B4RQN7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVGFVGWRG MVGSVLMQRM KEENDFAHIP EAFFFTSSNV GGAAPDFGQA AKTLLDANDV AELAKMDIIV TCQGGDYTKS VFQALRDSGW NGYWIDAASS LRMKDDAIIA LDPVNRNVID NGLKNGVKNY IGGNCTVSLM LMALGGLFQN DLVEWATSMT YQAASGAGAK NMRELISGMG AIHAQVADEL ADPSSAILDI DRKVSDFLRS EDYPKANFGV PLAGSLIPWI DVDLGNGQSK EEWKGGVETN KILGRSGNPT VIDGLCVRIG AMRCHSQAIT LKLKKDLPVS EIEAILAGAN DWVKVVPNEK EAGIRELTPA KVTGTLSVPV GRIRKLGMGG EYISAFTVGD QLLWGAAEPM RRVLRIVLGS L
NT Sequence
ATGAAAGTAG GTTTCGTCGG CTGGCGCGGT ATGGTCGGTT CGGTTTTGAT GCAGCGTATG AAAGAAGAAA ACGACTTCGC CCACATTCCC GAAGCGTTTT TCTTTACCTC TTCCAACGTC GGCGGCGCGG CACCTGATTT CGGTCAGGCG GCTAAAACAT TATTGGACGC GAACGACGTT GCCGAGCTGG CGAAAATGGA CATTATCGTC ACCTGCCAAG GCGGCGATTA CACCAAATCC GTCTTCCAAG CCCTGCGCGA CAGCGGCTGG AACGGCTACT GGATTGACGC GGCGTCCTCA CTGCGCATGA AAGACGACGC GATTATCGCC CTCGACCCCG TCAACCGCAA CGTCATCGAC AACGGTCTCA AAAACGGCGT GAAAAACTAC ATCGGCGGCA ACTGTACCGT CTCCCTGATG CTGATGGCTT TGGGCGGCCT GTTCCAAAAC GATTTGGTCG AATGGGCGAC CAGCATGACC TACCAAGCCG CTTCCGGCGC GGGCGCGAAA AATATGCGCG AACTCATCAG CGGCATGGGC GCGATTCACG CCCAAGTGGC GGACGAGCTT GCCGATCCTT CCAGCGCGAT TCTCGATATC GACCGCAAAG TGTCCGATTT CCTCCGCAGC GAAGACTATC CGAAAGCCAA CTTCGGCGTA CCGCTCGCCG GCAGCCTGAT TCCGTGGATT GACGTGGATT TGGGCAACGG CCAGTCCAAA GAAGAATGGA AGGGCGGCGT GGAAACCAAC AAAATCCTCG GACGCAGCGG CAATCCGACC GTCATCGACG GCCTGTGCGT CCGCATCGGC GCGATGCGCT GCCACAGCCA AGCCATCACT CTGAAGTTGA AAAAAGACCT GCCTGTTTCC GAAATCGAAG CGATTTTGGC AGGCGCGAAC GACTGGGTGA AAGTCGTCCC CAACGAAAAA GAAGCCGGCA TCCGCGAGCT GACGCCCGCC AAAGTTACCG GCACGCTGTC CGTCCCTGTC GGACGCATCC GCAAACTGGG CATGGGCGGC GAATACATCA GCGCGTTCAC CGTCGGCGAC CAACTTTTGT GGGGTGCAGC CGAACCGATG CGCCGCGTGT TGCGTATCGT GTTGGGCAGC CTGTAA
Details for NegoA.17885.a.B1.GE40071
| HARVESTED ON: | 3/24/2016 |
| SEQUENCED ON: | 3/25/2016 |
| EXPECTED MW: | 41kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMK VGFVGWRGMV GSVLMQRMKE ENDFAHIPEA FFFTSSNVGG AAPDFGQAAK TLLDANDVAE LAKMDIIVTC QGGDYTKSVF QALRDSGWNG YWIDAASSLR MKDDAIIALD PVNRNVIDNG LKNGVKNYIG GNCTVSLMLM ALGGLFQNDL VEWATSMTYQ AASGAGAKNM RELISGMGAI HAQVADELAD PSSAILDIDR KVSDFLRSED YPKANFGVPL AGSLIPWIDV DLGNGQSKEE WKGGVETNKI LGRSGNPTVI DGLCVRIGAM RCHSQAITLK LKKDLPVSEI EAILAGANDW VKVVPNEKEA GIRELTPAKV TGTLSVPVGR IRKLGMGGEY ISAFTVGDQL LWGAAEPMR
Validated NT Sequence
gcggcgcatc ggttcggctg caccccacaa aagttggtcg ccgacggtga acgcgctgat gtattcgccg cccatgccca gtttgcggat gcgtccgaca gggacggaca gcgtgccggt aactttggcg ggcgtcagct cgcggatgcc ggcttctttt tcgttgggga cgactttcac ccagtcgttc gcgcctgcca aaatcgcttc gatttcggaa acaggcaggt cttttttcaa cttcagagtg atggcttggc tgtggcagcg catcgcgccg atgcggacgc acaggccgtc gatgacggtc ggattgccgc tgcgtccgag gattttgttg gtttccacgc cgcccttcca ttcttctttg gactggccgt tgcccaaatc cacgtcaatc cacggaatca ggctgccggc gagcggtacg ccgaagttgg ctttcggata gtcttcgctg cggaggaaat cggacacttt gcggtcgata tcgagaatcg cgctggaagg atcggcaagc tcgtccgcca cttgggcgtg aatcgcgccc atgccgctga tgagttcgcg catatttttc gcgcccgcgc cggaagcggc ttggtaggtc atgctggtcg cccattcgac caaatcgttt tggaacaggc cgcccaaagc catcagcatc agggagacgg tacagttgcc gccgatgtag tttttcacgc cgtttttgag accgttgtcg atgacgttgc ggttgacggg gtcgagggcg ataatcgcgt cgtctttcat gcgcagtgag gacgccgcgt caatccagta gccgttccag ccgctgtcgc gcagggcttg gaagacggat ttggtgtaat cgccgccttg gcaggtgacg ataatgtcca ttttcgccag ctcggcaacg tcgttcgcgt ccaataatgt tttagccgcc tgaccgaaat caggtgccgc gccgccgacg ttggaagagg taaagaaaaa cgcttcggga atgtgggcga agtcgttttc ttctttcata cgctgcatca aaaccgaacc gaccataccg cgccagccga cgaaacctac tttcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMK VGFVGWRGMV GSVLMQRMKE ENDFAHIPEA FFFTSSNVGG AAPDFGQAAK TLLDANDVAE LAKMDIIVTC QGGDYTKSVF QALRDSGWNG YWIDAASSLR MKDDAIIALD PVNRNVIDNG LKNGVKNYIG GNCTVSLMLM ALGGLFQNDL VEWATSMTYQ AASGAGAKNM RELISGMGAI HAQVADELAD PSSAILDIDR KVSDFLRSED YPKANFGVPL AGSLIPWIDV DLGNGQSKEE WKGGVETNKI LGRSGNPTVI DGLCVRIGAM RCHSQAITLK LKKDLPVSEI EAILAGANDW VKVVPNEKEA GIRELTPAKV TGTLSVPVGR IRKLGMGGEY ISAFTVGDQL LWGAAEPMRR VLRIVLGSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag taggtttcgt cggctggcgc ggtatggtcg gttcggtttt gatgcagcgt atgaaagaag aaaacgactt cgcccacatt cccgaagcgt ttttctttac ctcttccaac gtcggcggcg cggcacctga tttcggtcag gcggctaaaa cattattgga cgcgaacgac gttgccgagc tggcgaaaat ggacattatc gtcacctgcc aaggcggcga ttacaccaaa tccgtcttcc aagccctgcg cgacagcggc tggaacggct actggattga cgcggcgtcc tcactgcgca tgaaagacga cgcgattatc gccctcgacc ccgtcaaccg caacgtcatc gacaacggtc tcaaaaacgg cgtgaaaaac tacatcggcg gcaactgtac cgtctccctg atgctgatgg ctttgggcgg cctgttccaa aacgatttgg tcgaatgggc gaccagcatg acctaccaag ccgcttccgg cgcgggcgcg aaaaatatgc gcgaactcat cagcggcatg ggcgcgattc acgcccaagt ggcggacgag cttgccgatc cttccagcgc gattctcgat atcgaccgca aagtgtccga tttcctccgc agcgaagact atccgaaagc caacttcggc gtaccgctcg ccggcagcct gattccgtgg attgacgtgg atttgggcaa cggccagtcc aaagaagaat ggaagggcgg cgtggaaacc aacaaaatcc tcggacgcag cggcaatccg accgtcatcg acggcctgtg cgtccgcatc ggcgcgatgc gctgccacag ccaagccatc actctgaagt tgaaaaaaga cctgcctgtt tccgaaatcg aagcgatttt ggcaggcgcg aacgactggg tgaaagtcgt ccccaacgaa aaagaagccg gcatccgcga gctgacgccc gccaaagtta ccggcacgct gtccgtccct gtcggacgca tccgcaaact gggcatgggc ggcgaataca tcagcgcgtt caccgtcggc gaccaacttt tgtggggtgc agccgaaccg atgcgccgcg tgttgcgtat cgtgttgggc agcctgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NegoA.17885.a.B1.PW37925
| PURIFICATION DATe: | 6/21/2016 |
| CONCENTRATION: | 21.5mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK VGFVGWRGMV GSVLMQRMKE ENDFAHIPEA FFFTSSNVGG AAPDFGQAAK TLLDANDVAE LAKMDIIVTC QGGDYTKSVF QALRDSGWNG YWIDAASSLR MKDDAIIALD PVNRNVIDNG LKNGVKNYIG GNCTVSLMLM ALGGLFQNDL VEWATSMTYQ AASGAGAKNM RELISGMGAI HAQVADELAD PSSAILDIDR KVSDFLRSED YPKANFGVPL AGSLIPWIDV DLGNGQSKEE WKGGVETNKI LGRSGNPTVI DGLCVRIGAM RCHSQAITLK LKKDLPVSEI EAILAGANDW VKVVPNEKEA GIRELTPAKV TGTLSVPVGR IRKLGMGGEY ISAFTVGDQL LWGAAEPMR
Validated NT Sequence
gcggcgcatc ggttcggctg caccccacaa aagttggtcg ccgacggtga acgcgctgat gtattcgccg cccatgccca gtttgcggat gcgtccgaca gggacggaca gcgtgccggt aactttggcg ggcgtcagct cgcggatgcc ggcttctttt tcgttgggga cgactttcac ccagtcgttc gcgcctgcca aaatcgcttc gatttcggaa acaggcaggt cttttttcaa cttcagagtg atggcttggc tgtggcagcg catcgcgccg atgcggacgc acaggccgtc gatgacggtc ggattgccgc tgcgtccgag gattttgttg gtttccacgc cgcccttcca ttcttctttg gactggccgt tgcccaaatc cacgtcaatc cacggaatca ggctgccggc gagcggtacg ccgaagttgg ctttcggata gtcttcgctg cggaggaaat cggacacttt gcggtcgata tcgagaatcg cgctggaagg atcggcaagc tcgtccgcca cttgggcgtg aatcgcgccc atgccgctga tgagttcgcg catatttttc gcgcccgcgc cggaagcggc ttggtaggtc atgctggtcg cccattcgac caaatcgttt tggaacaggc cgcccaaagc catcagcatc agggagacgg tacagttgcc gccgatgtag tttttcacgc cgtttttgag accgttgtcg atgacgttgc ggttgacggg gtcgagggcg ataatcgcgt cgtctttcat gcgcagtgag gacgccgcgt caatccagta gccgttccag ccgctgtcgc gcagggcttg gaagacggat ttggtgtaat cgccgccttg gcaggtgacg ataatgtcca ttttcgccag ctcggcaacg tcgttcgcgt ccaataatgt tttagccgcc tgaccgaaat caggtgccgc gccgccgacg ttggaagagg taaagaaaaa cgcttcggga atgtgggcga agtcgttttc ttctttcata cgctgcatca aaaccgaacc gaccataccg cgccagccga cgaaacctac tttcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMK VGFVGWRGMV GSVLMQRMKE ENDFAHIPEA FFFTSSNVGG AAPDFGQAAK TLLDANDVAE LAKMDIIVTC QGGDYTKSVF QALRDSGWNG YWIDAASSLR MKDDAIIALD PVNRNVIDNG LKNGVKNYIG GNCTVSLMLM ALGGLFQNDL VEWATSMTYQ AASGAGAKNM RELISGMGAI HAQVADELAD PSSAILDIDR KVSDFLRSED YPKANFGVPL AGSLIPWIDV DLGNGQSKEE WKGGVETNKI LGRSGNPTVI DGLCVRIGAM RCHSQAITLK LKKDLPVSEI EAILAGANDW VKVVPNEKEA GIRELTPAKV TGTLSVPVGR IRKLGMGGEY ISAFTVGDQL LWGAAEPMRR VLRIVLGSL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG TAGGTTTCGT CGGCTGGCGC GGTATGGTCG GTTCGGTTTT GATGCAGCGT ATGAAAGAAG AAAACGACTT CGCCCACATT CCCGAAGCGT TTTTCTTTAC CTCTTCCAAC GTCGGCGGCG CGGCACCTGA TTTCGGTCAG GCGGCTAAAA CATTATTGGA CGCGAACGAC GTTGCCGAGC TGGCGAAAAT GGACATTATC GTCACCTGCC AAGGCGGCGA TTACACCAAA TCCGTCTTCC AAGCCCTGCG CGACAGCGGC TGGAACGGCT ACTGGATTGA CGCGGCGTCC TCACTGCGCA TGAAAGACGA CGCGATTATC GCCCTCGACC CCGTCAACCG CAACGTCATC GACAACGGTC TCAAAAACGG CGTGAAAAAC TACATCGGCG GCAACTGTAC CGTCTCCCTG ATGCTGATGG CTTTGGGCGG CCTGTTCCAA AACGATTTGG TCGAATGGGC GACCAGCATG ACCTACCAAG CCGCTTCCGG CGCGGGCGCG AAAAATATGC GCGAACTCAT CAGCGGCATG GGCGCGATTC ACGCCCAAGT GGCGGACGAG CTTGCCGATC CTTCCAGCGC GATTCTCGAT ATCGACCGCA AAGTGTCCGA TTTCCTCCGC AGCGAAGACT ATCCGAAAGC CAACTTCGGC GTACCGCTCG CCGGCAGCCT GATTCCGTGG ATTGACGTGG ATTTGGGCAA CGGCCAGTCC AAAGAAGAAT GGAAGGGCGG CGTGGAAACC AACAAAATCC TCGGACGCAG CGGCAATCCG ACCGTCATCG ACGGCCTGTG CGTCCGCATC GGCGCGATGC GCTGCCACAG CCAAGCCATC ACTCTGAAGT TGAAAAAAGA CCTGCCTGTT TCCGAAATCG AAGCGATTTT GGCAGGCGCG AACGACTGGG TGAAAGTCGT CCCCAACGAA AAAGAAGCCG GCATCCGCGA GCTGACGCCC GCCAAAGTTA CCGGCACGCT GTCCGTCCCT GTCGGACGCA TCCGCAAACT GGGCATGGGC GGCGAATACA TCAGCGCGTT CACCGTCGGC GACCAACTTT TGTGGGGTGC AGCCGAACCG ATGCGCCGCG TGTTGCGTAT CGTGTTGGGC AGCCTGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T