NegoA.17876.a
GMP synthase [glutamine-hydrolyzing]
| CENTER ID: | NegoA.17876.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.17876.a.B1.GE40068 | full length | 1 | 521 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.17876.a.B1.PW37922 | full length | 1 | 521 |
Structures
5TW7
DEPOSITED: 11/11/2016
DETERMINATION: XRay
CLONE: NegoA.17876.a.B1.GE40068
PROTEIN: NegoA.17876.a.B1.PW37922
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.2863 |
| UniProt: | B4RJH7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQDKILILD FGSQVTRLIA RRVREAHVYC ELHSFDMPLD EIKAFNPKGI ILSGGPNSVY ESDYQADTGI FDLGIPVLGI CYGMQFMAHH LGGEVQPGNQ REFGYAQVKT IDSGLTRGIQ DDAPNTLDVW MSHGDKVSKL PDGFAVIGDT PSCPIAMMEN TEKQFYGIQF HPEVTHTKQG RALLNRFVLD ICGAQPGWTM PNYIEEAVAK IREQVGSDEV ILGLSGGVDS SVAAALIHRA IGDQLTCVFV DHGLLRLNEG KMVMDMFARN LGVKVIHVDA EGQFMAKLAG VTDPEKKRKI IGAEFIEVFD AEEKKLTNAK WLAQGTIYPD VIESAGAKTK KAHAIKSHHN VGGLPENMKL KLLEPLRDLF KDEVRELGVA LGLPREMVYR HPFPGPGLGV RILGEVKKEY ADLLRQADDI FIQELRNTTD ENGTSWYDLT SQAFAVFLPV KSVGVMGDGR TYYYVVALRA VITSDFMTAH WAELPYSLLG RVSNRIINEV KGINRVVYDV SGKPPATIEW E
NT Sequence
ATGACCCAAG ACAAAATCCT CATCCTCGAC TTCGGTTCGC AAGTTACCCG GCTGATCGCC CGCCGCGTGC GCGAAGCACA CGTTTACTGC GAGCTGCATT CCTTCGATAT GCCTTTGGAC GAAATCAAAG CCTTCAACCC CAAAGGCATC ATCCTTTCCG GCGGCCCTAA TTCTGTTTAC GAATCCGACT ATCAAGCCGA TACCGGTATT TTTGATTTGG GCATTCCGGT TTTGGGCATC TGCTACGGCA TGCAGTTTAT GGCGCACCAC TTGGGTGGCG AAGTGCAGCC CGGCAACCAG CGCGAATTCG GTTACGCGCA AGTCAAAACC ATCGACAGCG GACTGACACG CGGCATTCAA GACGACGCGC CCAACACACT CGACGTATGG ATGAGCCACG GCGACAAAGT GTCCAAACTG CCCGACGGTT TCGCCGTCAT CGGCGACACT CCGTCCTGCC CGATTGCCAT GATGGAAAAC ACCGAAAAAC AATTCTATGG CATCCAGTTC CACCCCGAAG TTACCCACAC CAAACAAGGC CGCGCCCTGT TGAACCGCTT TGTCTTGGAT ATTTGCGGCG CGCAACCGGG CTGGACGATG CCCAACTACA TCGAAGAAGC CGTTGCCAAA ATCCGCGAAC AAGTCGGCAG CGACGAAGTG ATTTTAGGTC TGTCCGGCGG CGTGGACTCT TCCGTAGCCG CCGCGCTGAT TCACCGCGCC ATCGGCGACC AACTGACCTG CGTGTTCGTC GATCACGGTT TGTTGCGCCT GAACGAAGGC AAAATGGTGA TGGATATGTT CGCCCGCAAC TTGGGTGTGA AAGTGATACA CGTCGATGCC GAAGGGCAGT TTATGGCGAA ACTCGCCGGC GTGACCGACC CTGAGAAAAA ACGCAAAATC ATCGGCGCGG AATTTATCGA AGTATTTGAT GCCGAAGAGA AAAAACTCAC CAACGCCAAA TGGCTGGCGC AAGGCACGAT TTACCCCGAC GTAATCGAAT CCGCCGGTGC GAAAACCAAA AAAGCCCACG CCATCAAATC CCACCACAAC GTCGGCGGCC TGCCTGAAAA TATGAAGCTC AAACTGCTTG AGCCCTTGCG CGACTTGTTC AAAGACGAAG TGCGCGAGTT GGGCGTGGCT TTGGGCCTGC CGCGCGAAAT GGTGTACCGC CACCCCTTCC CGGGCCCCGG TTTGGGTGTG CGCATCTTGG GCGAAGTGAA AAAAGAATAC GCCGACTTGC TGCGTCAGGC GGACGATATT TTCATCCAAG AATTACGCAA TACTACCGAC GAAAACGGCA CGTCTTGGTA TGACCTGACC AGCCAGGCAT TTGCCGTGTT CCTGCCCGTC AAATCCGTCG GCGTGATGGG CGACGGCCGC ACTTACTACT ACGTCGTCGC ACTGCGCGCA GTCATCACCA GCGACTTTAT GACTGCACAC TGGGCAGAGC TGCCATACTC ACTGCTCGGC CGCGTGTCCA ACCGCATCAT CAACGAAGTC AAAGGCATCA ACCGCGTGGT GTACGATGTC AGCGGCAAAC CGCCCGCCAC CATCGAGTGG GAATAA
Details for NegoA.17876.a.B1.GE40068
| HARVESTED ON: | 3/24/2016 |
| SEQUENCED ON: | 3/25/2016 |
| EXPECTED MW: | 59kDa |
| OBSERVED MW: | 59kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMT QDKILILDFG SQVTRLIARR VREAHVYCEL HSFDMPLDEI KAFNPKGIIL SGGPNSVYES DYQADTGIFD LGIPVLGICY GMQFMAHHLG GEVQPGNQRE FGYAQVKTID SGLTRGIQDD APNTLDVWMS HGDKVSKLPD GFAVIGDTPS CPIAMMENAE KQFYGIQFHP EVTHTKQGRA LLNRFVLDIC GAQPGWTMPN YIEEAVAKIR EQVGSDEVIL GLSGGVDSSV AAALIHRAIG DQLTCVFVDH GLLRLNEGKM VMDMFARNLG VKVIHVDAEG QFMAKLAGVT DPEKKRKIIG AEFIEVFDAE EKKLTNAKWL AQGTIYPDVI ESAGAKTKKA HAIKSHHNVG GLPENMKLKL LEPLRDLFKD EVRELGVALG LPREMVYRHP FPGPGLGVRI LGEVKKEYAD LLRQADDIFI QELRNTTDEN GTSWYDLTSQ AFAVFLPVKS VGVMGDGRTY DYVVALRAVI TSDFMTAHWA ELPYSLLGRV SNRIINEVKG INRVVYDVSG
Validated NT Sequence
tttgccgctg acatcgtaca ccacgcggtt gatgcctttg acttcgttga tgatgcggtt ggacacgcgg ccgagcagtg agtatggcag ctctgcccag tgtgcagtca taaagtcgct ggtgatgact gcgcgcagtg cgacgacgta gtcgtaagtg cggccgtcgc ccatcacgcc gacggatttg acgggcagga atacggcaaa tgcctggctg gtcaggtcat accaagacgt gccgttttcg tcggtagtat tgcgtaattc ttggatgaaa atatcgtccg cctgacgcag caagtcggcg tattcttttt tcacttcgcc caagatgcgc acacccaaac cggggcccgg gaaggggtgg cggtacacca tttcgcgcgg caggcccaaa gccacgccca actcgcgcac ttcgtctttg aacaagtcgc gcaagggctc aagcagtttg agcttcatat tttcaggcag gccgccgacg ttgtggtggg atttgatggc gtgggctttt ttggttttcg caccggcgga ttcgattacg tcggggtaaa tcgtgccttg cgccagccat ttggcgttgg tgagtttttt ctcttcggca tcaaatactt cgataaattc cgcgccgatg attttgcgtt ttttctcagg gtcggtcacg ccggcgagtt tcgccataaa ctgcccttcg gcatcgacgt gtatcacttt cacacccaag ttgcgggcga acatatccat caccattttg ccttcgttca ggcgcaacaa accgtgatcg acgaacacgc aggtcagttg gtcgccgatg gcgcggtgaa tcagcgcggc ggctacggaa gagtccacgc cgccggacag acctaaaatc acttcgtcgc tgccgacttg ttcgcggatt ttggcaacgg cttcttcgat gtagttgggc atcgtccagc ccggttgcgc gccgcaaata tccaagacaa agcggttcaa cagggcgcgg ccttgtttgg tgtgggtaac ttcggggtgg aactggatgc cgtagaattg tttttcggcg ttttccatca ttgcaatcgg gcaggacggg gtatcgccga tgacggcgaa accgtcgggc agtttggaca ctttgtcgcc gtggctcatc catacgtcga gtgtgttggg cgcgtcgtct tgaatgccgc gtgtcagtcc gctgtcgatg gttttgactt gcgcgtaacc gaattcgcgc tggttgccgg gctgcacttc gccacccaag tggtgcgcca taaactgcat gccgtagcag atgcccaaaa ccggaatgcc caaatcaaaa ataccggtat cggcttgata gtcggattcg taaacagaat tagggccgcc ggaaaggatg atgcctttgg ggttgaaggc tttgatttcg tccaaaggca tatcgaagga atgcagttcg cagtaaacgt gggcttcgcg cacgcggcgg gcaatcagcc gggtaacttg agaaccgaag tcgaggatga ggattttgtc ttgggtcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMT QDKILILDFG SQVTRLIARR VREAHVYCEL HSFDMPLDEI KAFNPKGIIL SGGPNSVYES DYQADTGIFD LGIPVLGICY GMQFMAHHLG GEVQPGNQRE FGYAQVKTID SGLTRGIQDD APNTLDVWMS HGDKVSKLPD GFAVIGDTPS CPIAMMENTE KQFYGIQFHP EVTHTKQGRA LLNRFVLDIC GAQPGWTMPN YIEEAVAKIR EQVGSDEVIL GLSGGVDSSV AAALIHRAIG DQLTCVFVDH GLLRLNEGKM VMDMFARNLG VKVIHVDAEG QFMAKLAGVT DPEKKRKIIG AEFIEVFDAE EKKLTNAKWL AQGTIYPDVI ESAGAKTKKA HAIKSHHNVG GLPENMKLKL LEPLRDLFKD EVRELGVALG LPREMVYRHP FPGPGLGVRI LGEVKKEYAD LLRQADDIFI QELRNTTDEN GTSWYDLTSQ AFAVFLPVKS VGVMGDGRTY YYVVALRAVI TSDFMTAHWA ELPYSLLGRV SNRIINEVKG INRVVYDVSG KPPATIEWE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc aagacaaaat cctcatcctc gacttcggtt cgcaagttac ccggctgatc gcccgccgcg tgcgcgaagc acacgtttac tgcgagctgc attccttcga tatgcctttg gacgaaatca aagccttcaa ccccaaaggc atcatccttt ccggcggccc taattctgtt tacgaatccg actatcaagc cgataccggt atttttgatt tgggcattcc ggttttgggc atctgctacg gcatgcagtt tatggcgcac cacttgggtg gcgaagtgca gcccggcaac cagcgcgaat tcggttacgc gcaagtcaaa accatcgaca gcggactgac acgcggcatt caagacgacg cgcccaacac actcgacgta tggatgagcc acggcgacaa agtgtccaaa ctgcccgacg gtttcgccgt catcggcgac actccgtcct gcccgattgc catgatggaa aacaccgaaa aacaattcta tggcatccag ttccaccccg aagttaccca caccaaacaa ggccgcgccc tgttgaaccg ctttgtcttg gatatttgcg gcgcgcaacc gggctggacg atgcccaact acatcgaaga agccgttgcc aaaatccgcg aacaagtcgg cagcgacgaa gtgattttag gtctgtccgg cggcgtggac tcttccgtag ccgccgcgct gattcaccgc gccatcggcg accaactgac ctgcgtgttc gtcgatcacg gtttgttgcg cctgaacgaa ggcaaaatgg tgatggatat gttcgcccgc aacttgggtg tgaaagtgat acacgtcgat gccgaagggc agtttatggc gaaactcgcc ggcgtgaccg accctgagaa aaaacgcaaa atcatcggcg cggaatttat cgaagtattt gatgccgaag agaaaaaact caccaacgcc aaatggctgg cgcaaggcac gatttacccc gacgtaatcg aatccgccgg tgcgaaaacc aaaaaagccc acgccatcaa atcccaccac aacgtcggcg gcctgcctga aaatatgaag ctcaaactgc ttgagccctt gcgcgacttg ttcaaagacg aagtgcgcga gttgggcgtg gctttgggcc tgccgcgcga aatggtgtac cgccacccct tcccgggccc cggtttgggt gtgcgcatct tgggcgaagt gaaaaaagaa tacgccgact tgctgcgtca ggcggacgat attttcatcc aagaattacg caatactacc gacgaaaacg gcacgtcttg gtatgacctg accagccagg catttgccgt gttcctgccc gtcaaatccg tcggcgtgat gggcgacggc cgcacttact actacgtcgt cgcactgcgc gcagtcatca ccagcgactt tatgactgca cactgggcag agctgccata ctcactgctc ggccgcgtgt ccaaccgcat catcaacgaa gtcaaaggca tcaaccgcgt ggtgtacgat gtcagcggca aaccgcccgc caccatcgag tgggaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NegoA.17876.a.B1.PW37922
| PURIFICATION DATe: | 6/13/2016 |
| CONCENTRATION: | 25.5mg/ml |
| OBSERVED MW: | 65kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT QDKILILDFG SQVTRLIARR VREAHVYCEL HSFDMPLDEI KAFNPKGIIL SGGPNSVYES DYQADTGIFD LGIPVLGICY GMQFMAHHLG GEVQPGNQRE FGYAQVKTID SGLTRGIQDD APNTLDVWMS HGDKVSKLPD GFAVIGDTPS CPIAMMENAE KQFYGIQFHP EVTHTKQGRA LLNRFVLDIC GAQPGWTMPN YIEEAVAKIR EQVGSDEVIL GLSGGVDSSV AAALIHRAIG DQLTCVFVDH GLLRLNEGKM VMDMFARNLG VKVIHVDAEG QFMAKLAGVT DPEKKRKIIG AEFIEVFDAE EKKLTNAKWL AQGTIYPDVI ESAGAKTKKA HAIKSHHNVG GLPENMKLKL LEPLRDLFKD EVRELGVALG LPREMVYRHP FPGPGLGVRI LGEVKKEYAD LLRQADDIFI QELRNTTDEN GTSWYDLTSQ AFAVFLPVKS VGVMGDGRTY DYVVALRAVI TSDFMTAHWA ELPYSLLGRV SNRIINEVKG INRVVYDVSG
Validated NT Sequence
tttgccgctg acatcgtaca ccacgcggtt gatgcctttg acttcgttga tgatgcggtt ggacacgcgg ccgagcagtg agtatggcag ctctgcccag tgtgcagtca taaagtcgct ggtgatgact gcgcgcagtg cgacgacgta gtcgtaagtg cggccgtcgc ccatcacgcc gacggatttg acgggcagga atacggcaaa tgcctggctg gtcaggtcat accaagacgt gccgttttcg tcggtagtat tgcgtaattc ttggatgaaa atatcgtccg cctgacgcag caagtcggcg tattcttttt tcacttcgcc caagatgcgc acacccaaac cggggcccgg gaaggggtgg cggtacacca tttcgcgcgg caggcccaaa gccacgccca actcgcgcac ttcgtctttg aacaagtcgc gcaagggctc aagcagtttg agcttcatat tttcaggcag gccgccgacg ttgtggtggg atttgatggc gtgggctttt ttggttttcg caccggcgga ttcgattacg tcggggtaaa tcgtgccttg cgccagccat ttggcgttgg tgagtttttt ctcttcggca tcaaatactt cgataaattc cgcgccgatg attttgcgtt ttttctcagg gtcggtcacg ccggcgagtt tcgccataaa ctgcccttcg gcatcgacgt gtatcacttt cacacccaag ttgcgggcga acatatccat caccattttg ccttcgttca ggcgcaacaa accgtgatcg acgaacacgc aggtcagttg gtcgccgatg gcgcggtgaa tcagcgcggc ggctacggaa gagtccacgc cgccggacag acctaaaatc acttcgtcgc tgccgacttg ttcgcggatt ttggcaacgg cttcttcgat gtagttgggc atcgtccagc ccggttgcgc gccgcaaata tccaagacaa agcggttcaa cagggcgcgg ccttgtttgg tgtgggtaac ttcggggtgg aactggatgc cgtagaattg tttttcggcg ttttccatca ttgcaatcgg gcaggacggg gtatcgccga tgacggcgaa accgtcgggc agtttggaca ctttgtcgcc gtggctcatc catacgtcga gtgtgttggg cgcgtcgtct tgaatgccgc gtgtcagtcc gctgtcgatg gttttgactt gcgcgtaacc gaattcgcgc tggttgccgg gctgcacttc gccacccaag tggtgcgcca taaactgcat gccgtagcag atgcccaaaa ccggaatgcc caaatcaaaa ataccggtat cggcttgata gtcggattcg taaacagaat tagggccgcc ggaaaggatg atgcctttgg ggttgaaggc tttgatttcg tccaaaggca tatcgaagga atgcagttcg cagtaaacgt gggcttcgcg cacgcggcgg gcaatcagcc gggtaacttg agaaccgaag tcgaggatga ggattttgtc ttgggtcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMT QDKILILDFG SQVTRLIARR VREAHVYCEL HSFDMPLDEI KAFNPKGIIL SGGPNSVYES DYQADTGIFD LGIPVLGICY GMQFMAHHLG GEVQPGNQRE FGYAQVKTID SGLTRGIQDD APNTLDVWMS HGDKVSKLPD GFAVIGDTPS CPIAMMENTE KQFYGIQFHP EVTHTKQGRA LLNRFVLDIC GAQPGWTMPN YIEEAVAKIR EQVGSDEVIL GLSGGVDSSV AAALIHRAIG DQLTCVFVDH GLLRLNEGKM VMDMFARNLG VKVIHVDAEG QFMAKLAGVT DPEKKRKIIG AEFIEVFDAE EKKLTNAKWL AQGTIYPDVI ESAGAKTKKA HAIKSHHNVG GLPENMKLKL LEPLRDLFKD EVRELGVALG LPREMVYRHP FPGPGLGVRI LGEVKKEYAD LLRQADDIFI QELRNTTDEN GTSWYDLTSQ AFAVFLPVKS VGVMGDGRTY YYVVALRAVI TSDFMTAHWA ELPYSLLGRV SNRIINEVKG INRVVYDVSG KPPATIEWE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC AAGACAAAAT CCTCATCCTC GACTTCGGTT CGCAAGTTAC CCGGCTGATC GCCCGCCGCG TGCGCGAAGC ACACGTTTAC TGCGAGCTGC ATTCCTTCGA TATGCCTTTG GACGAAATCA AAGCCTTCAA CCCCAAAGGC ATCATCCTTT CCGGCGGCCC TAATTCTGTT TACGAATCCG ACTATCAAGC CGATACCGGT ATTTTTGATT TGGGCATTCC GGTTTTGGGC ATCTGCTACG GCATGCAGTT TATGGCGCAC CACTTGGGTG GCGAAGTGCA GCCCGGCAAC CAGCGCGAAT TCGGTTACGC GCAAGTCAAA ACCATCGACA GCGGACTGAC ACGCGGCATT CAAGACGACG CGCCCAACAC ACTCGACGTA TGGATGAGCC ACGGCGACAA AGTGTCCAAA CTGCCCGACG GTTTCGCCGT CATCGGCGAC ACTCCGTCCT GCCCGATTGC CATGATGGAA AACACCGAAA AACAATTCTA TGGCATCCAG TTCCACCCCG AAGTTACCCA CACCAAACAA GGCCGCGCCC TGTTGAACCG CTTTGTCTTG GATATTTGCG GCGCGCAACC GGGCTGGACG ATGCCCAACT ACATCGAAGA AGCCGTTGCC AAAATCCGCG AACAAGTCGG CAGCGACGAA GTGATTTTAG GTCTGTCCGG CGGCGTGGAC TCTTCCGTAG CCGCCGCGCT GATTCACCGC GCCATCGGCG ACCAACTGAC CTGCGTGTTC GTCGATCACG GTTTGTTGCG CCTGAACGAA GGCAAAATGG TGATGGATAT GTTCGCCCGC AACTTGGGTG TGAAAGTGAT ACACGTCGAT GCCGAAGGGC AGTTTATGGC GAAACTCGCC GGCGTGACCG ACCCTGAGAA AAAACGCAAA ATCATCGGCG CGGAATTTAT CGAAGTATTT GATGCCGAAG AGAAAAAACT CACCAACGCC AAATGGCTGG CGCAAGGCAC GATTTACCCC GACGTAATCG AATCCGCCGG TGCGAAAACC AAAAAAGCCC ACGCCATCAA ATCCCACCAC AACGTCGGCG GCCTGCCTGA AAATATGAAG CTCAAACTGC TTGAGCCCTT GCGCGACTTG TTCAAAGACG AAGTGCGCGA GTTGGGCGTG GCTTTGGGCC TGCCGCGCGA AATGGTGTAC CGCCACCCCT TCCCGGGCCC CGGTTTGGGT GTGCGCATCT TGGGCGAAGT GAAAAAAGAA TACGCCGACT TGCTGCGTCA GGCGGACGAT ATTTTCATCC AAGAATTACG CAATACTACC GACGAAAACG GCACGTCTTG GTATGACCTG ACCAGCCAGG CATTTGCCGT GTTCCTGCCC GTCAAATCCG TCGGCGTGAT GGGCGACGGC CGCACTTACT ACTACGTCGT CGCACTGCGC GCAGTCATCA CCAGCGACTT TATGACTGCA CACTGGGCAG AGCTGCCATA CTCACTGCTC GGCCGCGTGT CCAACCGCAT CATCAACGAA GTCAAAGGCA TCAACCGCGT GGTGTACGAT GTCAGCGGCA AACCGCCCGC CACCATCGAG TGGGAATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T