SSGCID: Target Page

NegoA.17868.a

polyribonucleotide nucleotidyltransferase

CENTER ID:	NegoA.17868.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17868.a.B1.GE40067	full length		1	706

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17868.a.B1.PW37924	full length		1	706

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.533
UniProt:	B4RK29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFNKYVKTFQ YGNQTVTLET GEIARQAAAA VKVSMGDTVV FVAVTTNKEV KEGQDFFPLA VDYLERTYAA GKIPGGFFKR EGKQSEKEIL TSRLIDRPIR PLFPEGFYHD IQIVAMVVSV DPEIDSDIPA MLGASAALVL SGVPFAGPIG AARVGYINGV YVLNPTKAEL AKSQLDLVVA GTSKAVLMVE SEAKILPEDV MLGAVVYGHD QMQVAINAIN EFADEVNPEV WDWKAPETNE ELVAKVRGIA GETIKEAFKI RQKQARSAKL DEAWNAVKEA LITEETDTLA ANEIKGIFKH LEADVVRSQI LDGQPRIDGR DTRTVRPLNI QTGVLPRTHG SALFTRGETQ ALAVATLGTS RDEQIIDALS GEYTDRFMLH YNFPPYSTGE VGRMGAPKRR EIGHGRLAKR ALLAVLPKPE DFSYTMRVVS EITESNGSSS MASVCGGCLS LLSAGVPLKA HVAGIAMGLI LEGNKFAVLT DILGDEDHLG DMDFKVAGTT EGVTALQMDI KIQGITKEIM QIALAQAKEA RLHILDQMKA AVAGPQELSA HAPRLFTMKI SQDKIRDVIG KGGETIRSIT AETGTEINIA EDGTITIAAT TQEAGDAAKK RIEEITAEVE VGKVYEGTVV KILDNNVGAI VSVMPGKDGL VHISQIAHER VRNVGDYLQV GQVVNVKALE VDDRGRVRLS IKALLDAPVR EENAAE

NT Sequence

ATGTTCAATA AATACGTTAA GACCTTCCAA TACGGTAATC AAACCGTTAC TTTGGAAACC GGCGAAATTG CCCGCCAAGC TGCCGCTGCC GTTAAAGTAT CGATGGGCGA CACTGTTGTT TTTGTTGCCG TTACCACCAA CAAAGAGGTC AAAGAAGGTC AGGACTTCTT CCCGTTGGCC GTCGATTATT TGGAACGCAC TTATGCCGCA GGCAAAATCC CCGGCGGCTT CTTCAAACGC GAAGGCAAAC AAAGCGAAAA AGAAATCCTG ACCAGCCGTC TGATCGACCG CCCGATTCGT CCGCTGTTCC CCGAAGGTTT CTACCACGAC ATCCAAATCG TAGCGATGGT TGTGTCCGTC GATCCCGAAA TCGATTCCGA CATTCCTGCA ATGTTGGGCG CGTCCGCCGC GCTGGTGTTG AGCGGCGTAC CGTTCGCCGG CCCGATTGGC GCGGCGCGCG TGGGTTATAT AAACGGCGTG TACGTTTTGA ACCCGACTAA AGCCGAGTTG GCGAAATCAC AATTGGACTT GGTGGTCGCC GGTACTTCCA AAGCCGTGTT GATGGTGGAA TCCGAAGCCA AAATCCTGCC CGAAGACGTG ATGTTGGGCG CGGTGGTTTA CGGCCACGAT CAAATGCAGG TTGCCATCAA TGCAATCAAT GAATTTGCCG ACGAAGTGAA TCCGGAAGTT TGGGATTGGA AAGCACCTGA AACCAATGAA GAACTGGTTG CCAAAGTACG CGGGATTGCC GGCGAAACCA TTAAAGAAGC GTTCAAAATC CGTCAAAAAC AAGCGCGTTC CGCCAAATTG GACGAGGCTT GGAATGCGGT AAAAGAAGCC TTGATTACCG AAGAAACCGA CACGCTGGCA GCCAACGAAA TCAAAGGTAT TTTCAAACAC TTGGAAGCCG ATGTCGTCCG CAGCCAAATT TTGGACGGAC AACCGCGCAT CGACGGCCGC GACACCCGCA CCGTCCGTCC GCTGAACATC CAAACCGGCG TATTGCCGCG CACGCACGGT TCCGCACTGT TTACCCGTGG CGAAACCCAA GCCCTTGCCG TAGCGACTTT GGGTACTTCG CGCGACGAAC AAATCATCGA CGCGTTGTCC GGCGAATATA CCGACCGCTT TATGCTGCAC TACAACTTTC CGCCGTACTC TACCGGCGAA GTGGGCCGCA TGGGCGCGCC GAAACGCCGT GAAATCGGTC ACGGCCGTTT AGCTAAACGT GCGTTGTTGG CCGTATTGCC GAAACCTGAA GATTTCAGCT ACACCATGCG CGTGGTCTCC GAAATTACCG AATCCAACGG CTCTTCCTCT ATGGCTTCTG TCTGCGGCGG CTGTTTGAGC CTGCTGTCTG CCGGCGTGCC TTTGAAAGCA CACGTTGCCG GTATCGCGAT GGGTCTGATT TTGGAAGGCA ATAAGTTTGC CGTCCTGACC GACATTTTGG GCGACGAAGA CCACTTGGGC GATATGGACT TTAAAGTGGC CGGTACGACC GAAGGCGTTA CCGCGCTGCA AATGGACATC AAAATCCAAG GCATTACCAA AGAGATTATG CAAATTGCTT TGGCACAGGC CAAAGAAGCG CGTTTGCACA TCTTGGATCA GATGAAAGCT GCCGTTGCGG GTCCGCAAGA GCTGTCCGCA CACGCGCCGC GTTTGTTCAC CATGAAAATC AGCCAAGACA AAATCCGTGA TGTGATTGGT AAAGGCGGCG AAACCATTCG TTCGATTACC GCTGAAACCG GTACTGAAAT CAATATCGCT GAAGACGGTA CCATCACTAT CGCTGCGACT ACTCAAGAAG CGGGCGATGC AGCGAAAAAA CGCATCGAAG AAATCACTGC CGAAGTGGAA GTGGGCAAAG TGTACGAAGG TACTGTGGTT AAAATCCTCG ACAACAACGT CGGCGCGATT GTCAGCGTGA TGCCGGGCAA AGACGGTTTG GTACACATCA GCCAAATCGC ACACGAGCGC GTACGCAATG TCGGCGATTA CCTGCAAGTC GGTCAGGTGG TGAACGTGAA AGCATTGGAA GTGGATGACA GAGGCCGTGT CCGTCTGTCC ATCAAAGCCC TGCTGGATGC GCCTGTCCGT GAAGAAAATG CCGCCGAGTA A

Details for NegoA.17868.a.B1.GE40067

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	77kDa
OBSERVED MW:	77kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMF NKYVKTFQYG NQTVTLETGE IARQAAAAVK VSMGDTVVFV AVTTNKEVKE GQDFFPLAVD YLERTYAAGK IPGGFFKREG KQSEKEILTS RLIDRPIRPL FPEGFYHDIQ IVAMVVSVDP EIDSDIPAML GASAALVLSG VPFAGPIGAA RVGYINGVYV LNPTKAELAK SQLDLVVAGT SKAVLMVESE AKILPEDVML GAVVYGHDQM QVAINAINEF ADEVNPEVWD WKAPETNEEL VAKVRGIAGE TIKEAFKIRQ KQARSAKLDE AWNAVKEALI TEETDTLAAN EIKGIFKHLE ADVVRSQILD GQPRIDGRDT RTVRPLNIQT GVLPRTHGSA LFTRGETQAL AVATLGTSRD EQIIDALSGE YTDRFMLHYN FPPYSTGEVG RMGAPKRREI GHGRLAKRAL LAVLPKPEDF SYTMRVVSEI TESNGSSSMA SVCGGCLSLL SAGVPLKAHV AGIAMGLILE GNKFAVLTDI LGDEDHLGDM DFKVAGTTEG VTALQMDIKI QGITKEIMQI ALAQAKEARL HILDQMKAAV AGPQELSAHA PRLFTMKISQ DKIRDVIGKG GETIRSITAE TGTEINIAED GTITIAATTQ EAGDAAKKRI EEITAEVEVG KVYEGTVVKI LDNNVGAIVS VMPGKDGLVH ISQIAHERVR NVGDYLQVGQ VVNVKALEVD DRGRVRLSIK ALLDAPVREE NAAE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttca ataaatacgt taagaccttc caatacggta atcaaaccgt tactttggaa accggcgaaa ttgcccgcca agctgccgct gccgttaaag tatcgatggg cgacactgtt gtttttgttg ccgttaccac caacaaagag gtcaaagaag gtcaggactt cttcccgttg gccgtcgatt atttggaacg cacttatgcc gcaggcaaaa tccccggcgg cttcttcaaa cgcgaaggca aacaaagcga aaaagaaatc ctgaccagcc gtctgatcga ccgcccgatt cgtccgctgt tccccgaagg tttctaccac gacatccaaa tcgtagcgat ggttgtgtcc gtcgatcccg aaatcgattc cgacattcct gcaatgttgg gcgcgtccgc cgcgctggtg ttgagcggcg taccgttcgc cggcccgatt ggcgcggcgc gcgtgggtta tataaacggc gtgtacgttt tgaacccgac taaagccgag ttggcgaaat cacaattgga cttggtggtc gccggtactt ccaaagccgt gttgatggtg gaatccgaag ccaaaatcct gcccgaagac gtgatgttgg gcgcggtggt ttacggccac gatcaaatgc aggttgccat caatgcaatc aatgaatttg ccgacgaagt gaatccggaa gtttgggatt ggaaagcacc tgaaaccaat gaagaactgg ttgccaaagt acgcgggatt gccggcgaaa ccattaaaga agcgttcaaa atccgtcaaa aacaagcgcg ttccgccaaa ttggacgagg cttggaatgc ggtaaaagaa gccttgatta ccgaagaaac cgacacgctg gcagccaacg aaatcaaagg tattttcaaa cacttggaag ccgatgtcgt ccgcagccaa attttggacg gacaaccgcg catcgacggc cgcgacaccc gcaccgtccg tccgctgaac atccaaaccg gcgtattgcc gcgcacgcac ggttccgcac tgtttacccg tggcgaaacc caagcccttg ccgtagcgac tttgggtact tcgcgcgacg aacaaatcat cgacgcgttg tccggcgaat ataccgaccg ctttatgctg cactacaact ttccgccgta ctctaccggc gaagtgggcc gcatgggcgc gccgaaacgc cgtgaaatcg gtcacggccg tttagctaaa cgtgcgttgt tggccgtatt gccgaaacct gaagatttca gctacaccat gcgcgtggtc tccgaaatta ccgaatccaa cggctcttcc tctatggctt ctgtctgcgg cggctgtttg agcctgctgt ctgccggcgt gcctttgaaa gcacacgttg ccggtatcgc gatgggtctg attttggaag gcaataagtt tgccgtcctg accgacattt tgggcgacga agaccacttg ggcgatatgg actttaaagt ggccggtacg accgaaggcg ttaccgcgct gcaaatggac atcaaaatcc aaggcattac caaagagatt atgcaaattg ctttggcaca ggccaaagaa gcgcgtttgc acatcttgga tcagatgaaa gctgccgttg cgggtccgca agagctgtcc gcacacgcgc cgcgtttgtt caccatgaaa atcagccaag acaaaatccg tgatgtgatt ggtaaaggcg gcgaaaccat tcgttcgatt accgctgaaa ccggtactga aatcaatatc gctgaagacg gtaccatcac tatcgctgcg actactcaag aagcgggcga tgcagcgaaa aaacgcatcg aagaaatcac tgccgaagtg gaagtgggca aagtgtacga aggtactgtg gttaaaatcc tcgacaacaa cgtcggcgcg attgtcagcg tgatgccggg caaagacggt ttggtacaca tcagccaaat cgcacacgag cgcgtacgca atgtcggcga ttacctgcaa gtcggtcagg tggtgaacgt gaaagcattg gaagtggatg acagaggccg tgtccgtctg tccatcaaag ccctgctgga tgcgcctgtc cgtgaagaaa atgccgccga gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for NegoA.17868.a.B1.PW37924

PURIFICATION DATe:	6/21/2016
CONCENTRATION:	24mg/ml
OBSERVED MW:	80kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	24
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTCA ATAAATACGT TAAGACCTTC CAATACGGTA ATCAAACCGT TACTTTGGAA ACCGGCGAAA TTGCCCGCCA AGCTGCCGCT GCCGTTAAAG TATCGATGGG CGACACTGTT GTTTTTGTTG CCGTTACCAC CAACAAAGAG GTCAAAGAAG GTCAGGACTT CTTCCCGTTG GCCGTCGATT ATTTGGAACG CACTTATGCC GCAGGCAAAA TCCCCGGCGG CTTCTTCAAA CGCGAAGGCA AACAAAGCGA AAAAGAAATC CTGACCAGCC GTCTGATCGA CCGCCCGATT CGTCCGCTGT TCCCCGAAGG TTTCTACCAC GACATCCAAA TCGTAGCGAT GGTTGTGTCC GTCGATCCCG AAATCGATTC CGACATTCCT GCAATGTTGG GCGCGTCCGC CGCGCTGGTG TTGAGCGGCG TACCGTTCGC CGGCCCGATT GGCGCGGCGC GCGTGGGTTA TATAAACGGC GTGTACGTTT TGAACCCGAC TAAAGCCGAG TTGGCGAAAT CACAATTGGA CTTGGTGGTC GCCGGTACTT CCAAAGCCGT GTTGATGGTG GAATCCGAAG CCAAAATCCT GCCCGAAGAC GTGATGTTGG GCGCGGTGGT TTACGGCCAC GATCAAATGC AGGTTGCCAT CAATGCAATC AATGAATTTG CCGACGAAGT GAATCCGGAA GTTTGGGATT GGAAAGCACC TGAAACCAAT GAAGAACTGG TTGCCAAAGT ACGCGGGATT GCCGGCGAAA CCATTAAAGA AGCGTTCAAA ATCCGTCAAA AACAAGCGCG TTCCGCCAAA TTGGACGAGG CTTGGAATGC GGTAAAAGAA GCCTTGATTA CCGAAGAAAC CGACACGCTG GCAGCCAACG AAATCAAAGG TATTTTCAAA CACTTGGAAG CCGATGTCGT CCGCAGCCAA ATTTTGGACG GACAACCGCG CATCGACGGC CGCGACACCC GCACCGTCCG TCCGCTGAAC ATCCAAACCG GCGTATTGCC GCGCACGCAC GGTTCCGCAC TGTTTACCCG TGGCGAAACC CAAGCCCTTG CCGTAGCGAC TTTGGGTACT TCGCGCGACG AACAAATCAT CGACGCGTTG TCCGGCGAAT ATACCGACCG CTTTATGCTG CACTACAACT TTCCGCCGTA CTCTACCGGC GAAGTGGGCC GCATGGGCGC GCCGAAACGC CGTGAAATCG GTCACGGCCG TTTAGCTAAA CGTGCGTTGT TGGCCGTATT GCCGAAACCT GAAGATTTCA GCTACACCAT GCGCGTGGTC TCCGAAATTA CCGAATCCAA CGGCTCTTCC TCTATGGCTT CTGTCTGCGG CGGCTGTTTG AGCCTGCTGT CTGCCGGCGT GCCTTTGAAA GCACACGTTG CCGGTATCGC GATGGGTCTG ATTTTGGAAG GCAATAAGTT TGCCGTCCTG ACCGACATTT TGGGCGACGA AGACCACTTG GGCGATATGG ACTTTAAAGT GGCCGGTACG ACCGAAGGCG TTACCGCGCT GCAAATGGAC ATCAAAATCC AAGGCATTAC CAAAGAGATT ATGCAAATTG CTTTGGCACA GGCCAAAGAA GCGCGTTTGC ACATCTTGGA TCAGATGAAA GCTGCCGTTG CGGGTCCGCA AGAGCTGTCC GCACACGCGC CGCGTTTGTT CACCATGAAA ATCAGCCAAG ACAAAATCCG TGATGTGATT GGTAAAGGCG GCGAAACCAT TCGTTCGATT ACCGCTGAAA CCGGTACTGA AATCAATATC GCTGAAGACG GTACCATCAC TATCGCTGCG ACTACTCAAG AAGCGGGCGA TGCAGCGAAA AAACGCATCG AAGAAATCAC TGCCGAAGTG GAAGTGGGCA AAGTGTACGA AGGTACTGTG GTTAAAATCC TCGACAACAA CGTCGGCGCG ATTGTCAGCG TGATGCCGGG CAAAGACGGT TTGGTACACA TCAGCCAAAT CGCACACGAG CGCGTACGCA ATGTCGGCGA TTACCTGCAA GTCGGTCAGG TGGTGAACGT GAAAGCATTG GAAGTGGATG ACAGAGGCCG TGTCCGTCTG TCCATCAAAG CCCTGCTGGA TGCGCCTGTC CGTGAAGAAA ATGCCGCCGA GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT