NegoA.17669.a

dTDP-4-dehydrorhamnose reductase

CENTER ID:	NegoA.17669.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17669.a.B1.GE40066	full length		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17669.a.B1.PS37966	full length		1	287

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|521006.8.peg.530
UniProt:	B4RK26

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRILLTGSKS QLAHCLRDRL PEDWETIATD SASLDITDAD AVCNMVKSFQ PDAIVNTAAY TAVDKAEGDA AAAFAVNASA VYNLALAAHR AHARFIHIST DYVFDGKGKI PHQESDFTNP SNVYGQSKTA GELLALSANP DSLILRTSWL FSEYGDNFVR TMLNLARERS PLSAVHNQIG CPTYAGDLSA AIIRLLQQSN PVRGIYHYAG GKSVSWYEFA RHIFQTALQQ DASFPVPELK AVSDEGSAAA APRPAYSILD CRKIENDFGI KPSDWQKALA QVVSKLL

NT Sequence

ATGAGAATCC TATTGACAGG CTCGAAAAGC CAACTGGCAC ACTGCCTGCG CGACCGTCTT CCTGAAGACT GGGAAACCAT TGCGACGGAT TCCGCATCCC TAGACATTAC CGATGCCGAT GCCGTCTGCA ACATGGTCAA AAGTTTCCAA CCCGACGCTA TTGTCAACAC GGCTGCCTAT ACTGCCGTCG ACAAAGCGGA AGGCGATGCG GCAGCGGCAT TTGCCGTCAA TGCTTCCGCC GTTTACAACC TTGCCTTGGC AGCACATCGC GCCCATGCCC GATTCATCCA CATCTCAACC GACTATGTCT TTGACGGAAA AGGGAAAATA CCCCATCAGG AAAGCGACTT TACCAACCCT TCCAATGTAT ACGGACAATC CAAAACCGCC GGCGAACTGC TCGCACTGTC TGCCAATCCC GACAGCCTTA TCCTGCGGAC TTCTTGGCTG TTTAGCGAAT ACGGGGACAA CTTTGTCCGC ACGATGCTGA ACCTTGCGCG GGAACGTTCC CCGCTGTCCG CCGTCCACAA CCAAATCGGC TGCCCGACCT ATGCCGGCGA CTTGTCCGCC GCCATCATCC GCCTGTTGCA GCAGTCCAAT CCCGTTCGCG GCATTTACCA CTACGCCGGC GGCAAATCCG TATCCTGGTA CGAATTTGCC CGGCACATCT TTCAGACGGC ATTGCAACAG GATGCCTCTT TTCCCGTTCC CGAACTGAAA GCCGTTTCGG ATGAAGGCTC TGCGGCTGCC GCCCCCAGGC CCGCATACAG CATTTTGGAC TGCCGCAAAA TCGAAAACGA CTTCGGCATC AAACCGTCAG ACTGGCAAAA AGCCCTTGCA CAGGTCGTTT CCAAGCTGCT CTGA

Details for NegoA.17669.a.B1.GE40066

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR ILLTGSKSQL AHCLRDRLPE DWETIATDSA SLDITDADAV CNMVKSFQPD AIVNTAAYTA VDKAEGDAAA AFAVNASAVY NLALAAHRAH ARFIHISTDY VFDGKGKIPY QESDFTNPSN VYGQSKTAGE LLALSANPDS LILRTSWLFS EYGDNFVRTM LNLARERSPL SAVHNQIGCP TYAGDLSAAI IRLLQQSNPV RGIYHYAGGK SVSWYEFARH IFQTALQQDA SFPVPELKAV SDEGSAAAAP RPAYSILDCR KIENDFGIKP SDWQKALXXX VSKLL

Validated NT Sequence

ttactcactt agagcagctt ggaaacganc tnnncnaggg ctttttgcca gtctgacggt ttgatgccga agtcgttttc gattttgcgg cagtccaaaa tgctgtatgc gggcctgggg gcggcagccg cagagccttc atccgaaacg gctttcagtt cgggaacggg aaaagaggca tcctgttgca atgccgtctg aaagatgtgc cgggcaaatt cgtaccagga tacggatttg ccgccggcgt agtggtaaat gccgcgaacg ggattggact gctgcaacag gcggatgatg gcggcggaca agtcgccggc ataggtcggg cagccgattt ggttgtggac ggcggacagc ggggaacgtt cccgcgcaag gttcagcatc gtgcggacaa agttgtcccc gtattcgcta aacagccaag aagtccgcag gataaggctg tcgggattgg cagacagtgc gagcagttcg ccggcggttt tggattgtcc gtatacattg gaagggttgg taaagtcgct ttcctgatag ggtattttcc cttttccgtc aaagacatag tcggttgaga tgtggatgaa tcgggcatgg gcgcgatgtg ctgccaaggc aaggttgtaa acggcggaag cattgacggc aaatgccgct gccgcatcgc cttccgcttt gtcgacggca gtataggcag ccgtgttgac aatagcgtcg ggttggaaac ttttgaccat gttgcagacg gcatcggcat cggtaatgtc tagggatgcg gaatccgtcg caatggtttc ccagtcttca ggaagacggt cgcgcaggca gtgtgccagt tggcttttcg agcctgtcaa taggattctc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMR ILLTGSKSQL AHCLRDRLPE DWETIATDSA SLDITDADAV CNMVKSFQPD AIVNTAAYTA VDKAEGDAAA AFAVNASAVY NLALAAHRAH ARFIHISTDY VFDGKGKIPH QESDFTNPSN VYGQSKTAGE LLALSANPDS LILRTSWLFS EYGDNFVRTM LNLARERSPL SAVHNQIGCP TYAGDLSAAI IRLLQQSNPV RGIYHYAGGK SVSWYEFARH IFQTALQQDA SFPVPELKAV SDEGSAAAAP RPAYSILDCR KIENDFGIKP SDWQKALAQV VSKLL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagaa tcctattgac aggctcgaaa agccaactgg cacactgcct gcgcgaccgt cttcctgaag actgggaaac cattgcgacg gattccgcat ccctagacat taccgatgcc gatgccgtct gcaacatggt caaaagtttc caacccgacg ctattgtcaa cacggctgcc tatactgccg tcgacaaagc ggaaggcgat gcggcagcgg catttgccgt caatgcttcc gccgtttaca accttgcctt ggcagcacat cgcgcccatg cccgattcat ccacatctca accgactatg tctttgacgg aaaagggaaa ataccccatc aggaaagcga ctttaccaac ccttccaatg tatacggaca atccaaaacc gccggcgaac tgctcgcact gtctgccaat cccgacagcc ttatcctgcg gacttcttgg ctgtttagcg aatacgggga caactttgtc cgcacgatgc tgaaccttgc gcgggaacgt tccccgctgt ccgccgtcca caaccaaatc ggctgcccga cctatgccgg cgacttgtcc gccgccatca tccgcctgtt gcagcagtcc aatcccgttc gcggcattta ccactacgcc ggcggcaaat ccgtatcctg gtacgaattt gcccggcaca tctttcagac ggcattgcaa caggatgcct cttttcccgt tcccgaactg aaagccgttt cggatgaagg ctctgcggct gccgccccca ggcccgcata cagcattttg gactgccgca aaatcgaaaa cgacttcggc atcaaaccgt cagactggca aaaagccctt gcacaggtcg tttccaagct gctctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for NegoA.17669.a.B1.PS37966

PURIFICATION DATe:	7/6/2016
CONCENTRATION:	16.75mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	23
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGAA TCCTATTGAC AGGCTCGAAA AGCCAACTGG CACACTGCCT GCGCGACCGT CTTCCTGAAG ACTGGGAAAC CATTGCGACG GATTCCGCAT CCCTAGACAT TACCGATGCC GATGCCGTCT GCAACATGGT CAAAAGTTTC CAACCCGACG CTATTGTCAA CACGGCTGCC TATACTGCCG TCGACAAAGC GGAAGGCGAT GCGGCAGCGG CATTTGCCGT CAATGCTTCC GCCGTTTACA ACCTTGCCTT GGCAGCACAT CGCGCCCATG CCCGATTCAT CCACATCTCA ACCGACTATG TCTTTGACGG AAAAGGGAAA ATACCCCATC AGGAAAGCGA CTTTACCAAC CCTTCCAATG TATACGGACA ATCCAAAACC GCCGGCGAAC TGCTCGCACT GTCTGCCAAT CCCGACAGCC TTATCCTGCG GACTTCTTGG CTGTTTAGCG AATACGGGGA CAACTTTGTC CGCACGATGC TGAACCTTGC GCGGGAACGT TCCCCGCTGT CCGCCGTCCA CAACCAAATC GGCTGCCCGA CCTATGCCGG CGACTTGTCC GCCGCCATCA TCCGCCTGTT GCAGCAGTCC AATCCCGTTC GCGGCATTTA CCACTACGCC GGCGGCAAAT CCGTATCCTG GTACGAATTT GCCCGGCACA TCTTTCAGAC GGCATTGCAA CAGGATGCCT CTTTTCCCGT TCCCGAACTG AAAGCCGTTT CGGATGAAGG CTCTGCGGCT GCCGCCCCCA GGCCCGCATA CAGCATTTTG GACTGCCGCA AAATCGAAAA CGACTTCGGC ATCAAACCGT CAGACTGGCA AAAAGCCCTT GCACAGGTCG TTTCCAAGCT GCTCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT