SSGCID: Target Page

NegoA.17298.a

oxygen-independent coproporphyrinogen III oxidase

CENTER ID:	NegoA.17298.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17298.a.B1.GE40063	full length		1	473

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17298.a.B1.PS37964	full length		1	473

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.2013
UniProt:	B4RQI3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIIQIQNNH NVNDDRPEFD RALIASLPAS GPRYTSYPTA DRFHDGFREG EYIKVLHLRG MGALNKPLSL YIHIPFCNTI CYYCGCNKII TKDKSRADTY IEYLEKEMEL LAPHLNGRHQ LAQLHFGGGT PTFLSDEQIE RVFRMIRKHF ELIPSGEYSI EIDPRKVSRD TVLMLGRLGF NRMSVGIQDF DPKVQAAVNR IQSYEETKEV IDAAREAGFK SVSVDLIYGL PHQTSESIKT TIDTVLSLDP DRLALYHYAH LPHVFKPQRR IDTAAVPDSE EKLDMLQYCV QTLTERGYVF IGMDHFAKPG DELSIALKEG FLQRNFQGYS TYADCDLVAI GVSSIGKIGS TYSQNERDID AYYAAIDEGR LPIMRGYQLN QDDILRRNII QDLMCRFALD YRIYESMFGI PFDRYFKDEL ADLEKLAGLG LVRLNSHGLT VTPKGRFLIR NIAMVFDYHL RHKETKAKYS QTV

NT Sequence

ATGAAAATCA TTCAGATACA GAACAATCAC AATGTAAACG ATGACCGCCC CGAGTTTGAC CGCGCGCTGA TTGCCAGCCT GCCCGCCAGC GGTCCGCGCT ATACTTCCTA CCCTACCGCC GACCGTTTCC ATGACGGTTT CCGCGAAGGC GAATATATCA AAGTTTTACA TTTGCGCGGT ATGGGCGCGT TAAACAAACC GCTTTCCCTT TACATTCACA TTCCGTTTTG CAACACCATC TGCTACTACT GCGGCTGCAA CAAAATCATC ACCAAAGACA AAAGCCGCGC CGATACCTAC ATCGAATATC TTGAAAAAGA AATGGAACTG CTCGCTCCAC ATCTGAACGG ACGGCACCAG CTTGCCCAAC TGCACTTCGG CGGCGGCACA CCGACCTTTT TGAGCGACGA ACAGATCGAA CGTGTCTTCC GCATGATCCG CAAACATTTC GAACTGATAC CGTCCGGCGA ATATTCCATC GAAATCGACC CGCGCAAAGT CAGCCGCGAC ACCGTCCTCA TGCTCGGCAG ACTCGGCTTC AACCGCATGA GCGTCGGCAT TCAGGATTTC GACCCCAAAG TGCAGGCGGC AGTCAACCGC ATCCAAAGCT ACGAAGAAAC CAAAGAAGTC ATCGATGCCG CCCGTGAGGC AGGGTTCAAA TCCGTCAGCG TCGATTTGAT TTACGGCTTG CCGCATCAGA CTTCCGAAAG CATCAAAACC ACCATAGATA CCGTTTTGTC GCTCGATCCC GACCGCCTCG CCCTTTACCA CTACGCCCAC CTGCCGCACG TGTTCAAACC GCAACGCCGC ATCGATACCG CCGCCGTCCC CGACAGCGAA GAGAAGCTCG ATATGCTGCA ATACTGCGTC CAAACCCTGA CAGAGCGCGG CTACGTCTTC ATCGGCATGG ACCATTTCGC CAAACCTGGC GACGAACTCT CCATCGCCCT CAAAGAAGGC TTCCTCCAAC GCAACTTCCA AGGCTATTCG ACCTACGCGG ATTGCGATTT GGTCGCCATC GGCGTGTCGT CCATCGGCAA AATCGGCAGC ACCTATTCCC AAAACGAACG CGACATCGAT GCCTACTATG CCGCCATCGA CGAAGGCAGA CTGCCCATCA TGCGCGGCTA CCAGCTCAAT CAGGACGACA TCCTGCGCCG CAACATCATT CAGGATTTAA TGTGCCGCTT CGCGCTCGAC TACAGGATTT ACGAAAGTAT GTTCGGCATC CCGTTCGACC GCTACTTCAA AGACGAACTG GCGGATTTGG AAAAACTCGC CGGTTTGGGA TTGGTGCGCC TGAACAGCCA CGGGCTGACC GTTACACCGA AAGGACGCTT CCTCATCCGC AACATCGCCA TGGTCTTCGA CTACCACCTG CGCCACAAAG AAACCAAAGC GAAATATTCG CAAACGGTGT GA

Details for NegoA.17298.a.B1.GE40063

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMK IIQIQNNHNV NDDRPEFDRA LIASLPASGP RYTSYPTADR FHDGFREGEY IKVLHLRGMG ALNKPLSLYI HIPFCNTICY YCGCNKIITK DKSRADTYIE YLEKEMELLA PHLNGRHQLA QLHFGGGTPT FLSDEQIERV FRMIRKHFEL IPSGEYSIEI DPRKVSRDTV LMLGRLGFNR MSVGIQDFDP KVQAAVNRIQ SYEETKEVID AAREAGFKSV SVDLIYGLPH QTSESIKTTI DTVLSLDPDR LALYHYAHLP HVFKPQRRID TAAVPDSEEK LDMLQYCVQT LTERGYVFIG MDHFAKPDDE LSIALKEGFL QRNFQGYSTY ADCDLVAIGV SSIGKIGSTY SQNERDIDAY YAAIDEGRLP IMRGYQLNQD DILRRNIIQD LMCRFALDYR IYESMFGIPF DRYFKDELAD LEKLAGLGLV RLNSHGLTVT PKGRFLIRNI AMVFDYHLRH KET

Validated NT Sequence

tggtttcttt gtggcgcagg tggtagtcga agaccatggc gatgttgcgg atgaggaagc gtcctttcgg tgtaacggtc agcccgtggc tgttcaggcg caccaatccc aaaccggcga gtttttccaa atccgccagt tcgtctttga agtagcggtc gaacgggatg ccgaacatac tttcgtaaat cctgtagtcg agcgcgaagc ggcacattaa atcctgaatg atgttgcggc gcaggatgtc gtcctgattg agctggtagc cgcgcatgat gggcagtctg ccttcgtcga tggcggcata gtaggcatcg atgtcgcgtt cgttttggga ataggtgctg ccgattttgc cgatggacga cacgccgatg gcgaccaaat cgcaatccgc gtaggtcgaa tagccttgga agttgcgttg gaggaagcct tctttgaggg cgatggagag ttcgtcgtca ggtttggcga aatggtccat gccgatgaag acgtagccgc gctctgtcag ggtttggacg cagtattgca gcatatcgag cttctcttcg ctgtcgggga cggcggcggt atcgatgcgg cgttgcggtt tgaacacgtg cggcaggtgg gcgtagtggt aaagggcgag gcggtcggga tcgagcgaca aaacggtatc tatggtggtt ttgatgcttt cggaagtctg atgcggcaag ccgtaaatca aatcgacgct gacggatttg aaccctgcct cacgggcggc atcgatgact tctttggttt cttcgtagct ttggatgcgg ttgactgccg cctgcacttt ggggtcgaaa tcctgaatgc cgacgctcat gcggttgaag ccgagtctgc cgagcatgag gacggtgtcg cggctgactt tgcgcgggtc gatttcgatg gaatattcgc cggacggtat cagttcgaaa tgtttgcgga tcatgcggaa gacacgttcg atctgttcgt cgctcaaaaa ggtcggcgtg ccgccgccga agtgcagttg ggcaagctgg tgccgtccgt tcagatgtgg agcgagcagt tccatttctt tttcaagata ttcgatgtag gtatcggcgc ggcttttgtc tttggtgatg attttgttgc agccgcagta gtagcagatg gtgttgcaaa acggaatgtg aatgtaaagg gaaagcggtt tgtttaacgc gcccataccg cgcaaatgta aaactttgat atattcgcct tcgcggaaac cgtcatggaa acggtcggcg gtagggtagg aagtatagcg cgggccgctg gcgggcaggc tggcaatcag cgcgcggtca aactcggggc ggtcatcgtt tacattgtga ttgttctgta tctgaatgat tttcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHMK IIQIQNNHNV NDDRPEFDRA LIASLPASGP RYTSYPTADR FHDGFREGEY IKVLHLRGMG ALNKPLSLYI HIPFCNTICY YCGCNKIITK DKSRADTYIE YLEKEMELLA PHLNGRHQLA QLHFGGGTPT FLSDEQIERV FRMIRKHFEL IPSGEYSIEI DPRKVSRDTV LMLGRLGFNR MSVGIQDFDP KVQAAVNRIQ SYEETKEVID AAREAGFKSV SVDLIYGLPH QTSESIKTTI DTVLSLDPDR LALYHYAHLP HVFKPQRRID TAAVPDSEEK LDMLQYCVQT LTERGYVFIG MDHFAKPGDE LSIALKEGFL QRNFQGYSTY ADCDLVAIGV SSIGKIGSTY SQNERDIDAY YAAIDEGRLP IMRGYQLNQD DILRRNIIQD LMCRFALDYR IYESMFGIPF DRYFKDELAD LEKLAGLGLV RLNSHGLTVT PKGRFLIRNI AMVFDYHLRH KETKAKYSQT V

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tcattcagat acagaacaat cacaatgtaa acgatgaccg ccccgagttt gaccgcgcgc tgattgccag cctgcccgcc agcggtccgc gctatacttc ctaccctacc gccgaccgtt tccatgacgg tttccgcgaa ggcgaatata tcaaagtttt acatttgcgc ggtatgggcg cgttaaacaa accgctttcc ctttacattc acattccgtt ttgcaacacc atctgctact actgcggctg caacaaaatc atcaccaaag acaaaagccg cgccgatacc tacatcgaat atcttgaaaa agaaatggaa ctgctcgctc cacatctgaa cggacggcac cagcttgccc aactgcactt cggcggcggc acaccgacct ttttgagcga cgaacagatc gaacgtgtct tccgcatgat ccgcaaacat ttcgaactga taccgtccgg cgaatattcc atcgaaatcg acccgcgcaa agtcagccgc gacaccgtcc tcatgctcgg cagactcggc ttcaaccgca tgagcgtcgg cattcaggat ttcgacccca aagtgcaggc ggcagtcaac cgcatccaaa gctacgaaga aaccaaagaa gtcatcgatg ccgcccgtga ggcagggttc aaatccgtca gcgtcgattt gatttacggc ttgccgcatc agacttccga aagcatcaaa accaccatag ataccgtttt gtcgctcgat cccgaccgcc tcgcccttta ccactacgcc cacctgccgc acgtgttcaa accgcaacgc cgcatcgata ccgccgccgt ccccgacagc gaagagaagc tcgatatgct gcaatactgc gtccaaaccc tgacagagcg cggctacgtc ttcatcggca tggaccattt cgccaaacct ggcgacgaac tctccatcgc cctcaaagaa ggcttcctcc aacgcaactt ccaaggctat tcgacctacg cggattgcga tttggtcgcc atcggcgtgt cgtccatcgg caaaatcggc agcacctatt cccaaaacga acgcgacatc gatgcctact atgccgccat cgacgaaggc agactgccca tcatgcgcgg ctaccagctc aatcaggacg acatcctgcg ccgcaacatc attcaggatt taatgtgccg cttcgcgctc gactacagga tttacgaaag tatgttcggc atcccgttcg accgctactt caaagacgaa ctggcggatt tggaaaaact cgccggtttg ggattggtgc gcctgaacag ccacgggctg accgttacac cgaaaggacg cttcctcatc cgcaacatcg ccatggtctt cgactaccac ctgcgccaca aagaaaccaa agcgaaatat tcgcaaacgg tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for NegoA.17298.a.B1.PS37964

PURIFICATION DATe:	6/29/2016
CONCENTRATION:	19.58mg/ml
OBSERVED MW:	52kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMK IIQIQNNHNV NDDRPEFDRA LIASLPASGP RYTSYPTADR FHDGFREGEY IKVLHLRGMG ALNKPLSLYI HIPFCNTICY YCGCNKIITK DKSRADTYIE YLEKEMELLA PHLNGRHQLA QLHFGGGTPT FLSDEQIERV FRMIRKHFEL IPSGEYSIEI DPRKVSRDTV LMLGRLGFNR MSVGIQDFDP KVQAAVNRIQ SYEETKEVID AAREAGFKSV SVDLIYGLPH QTSESIKTTI DTVLSLDPDR LALYHYAHLP HVFKPQRRID TAAVPDSEEK LDMLQYCVQT LTERGYVFIG MDHFAKPGDE LSIALKEGFL QRNFQGYSTY ADCDLVAIGV SSIGKIGSTY SQNERDIDAY YAAIDEGRLP IMRGYQLNQD DILRRNIIQD LMCRFALDYR IYESMFGIPF DRYFKDELAD LEKLAGLGLV RLNSHGLTVT PKGRFLIRNI AMVFDYHLRH KETKAKYSQT V

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA TCATTCAGAT ACAGAACAAT CACAATGTAA ACGATGACCG CCCCGAGTTT GACCGCGCGC TGATTGCCAG CCTGCCCGCC AGCGGTCCGC GCTATACTTC CTACCCTACC GCCGACCGTT TCCATGACGG TTTCCGCGAA GGCGAATATA TCAAAGTTTT ACATTTGCGC GGTATGGGCG CGTTAAACAA ACCGCTTTCC CTTTACATTC ACATTCCGTT TTGCAACACC ATCTGCTACT ACTGCGGCTG CAACAAAATC ATCACCAAAG ACAAAAGCCG CGCCGATACC TACATCGAAT ATCTTGAAAA AGAAATGGAA CTGCTCGCTC CACATCTGAA CGGACGGCAC CAGCTTGCCC AACTGCACTT CGGCGGCGGC ACACCGACCT TTTTGAGCGA CGAACAGATC GAACGTGTCT TCCGCATGAT CCGCAAACAT TTCGAACTGA TACCGTCCGG CGAATATTCC ATCGAAATCG ACCCGCGCAA AGTCAGCCGC GACACCGTCC TCATGCTCGG CAGACTCGGC TTCAACCGCA TGAGCGTCGG CATTCAGGAT TTCGACCCCA AAGTGCAGGC GGCAGTCAAC CGCATCCAAA GCTACGAAGA AACCAAAGAA GTCATCGATG CCGCCCGTGA GGCAGGGTTC AAATCCGTCA GCGTCGATTT GATTTACGGC TTGCCGCATC AGACTTCCGA AAGCATCAAA ACCACCATAG ATACCGTTTT GTCGCTCGAT CCCGACCGCC TCGCCCTTTA CCACTACGCC CACCTGCCGC ACGTGTTCAA ACCGCAACGC CGCATCGATA CCGCCGCCGT CCCCGACAGC GAAGAGAAGC TCGATATGCT GCAATACTGC GTCCAAACCC TGACAGAGCG CGGCTACGTC TTCATCGGCA TGGACCATTT CGCCAAACCT GGCGACGAAC TCTCCATCGC CCTCAAAGAA GGCTTCCTCC AACGCAACTT CCAAGGCTAT TCGACCTACG CGGATTGCGA TTTGGTCGCC ATCGGCGTGT CGTCCATCGG CAAAATCGGC AGCACCTATT CCCAAAACGA ACGCGACATC GATGCCTACT ATGCCGCCAT CGACGAAGGC AGACTGCCCA TCATGCGCGG CTACCAGCTC AATCAGGACG ACATCCTGCG CCGCAACATC ATTCAGGATT TAATGTGCCG CTTCGCGCTC GACTACAGGA TTTACGAAAG TATGTTCGGC ATCCCGTTCG ACCGCTACTT CAAAGACGAA CTGGCGGATT TGGAAAAACT CGCCGGTTTG GGATTGGTGC GCCTGAACAG CCACGGGCTG ACCGTTACAC CGAAAGGACG CTTCCTCATC CGCAACATCG CCATGGTCTT CGACTACCAC CTGCGCCACA AAGAAACCAA AGCGAAATAT TCGCAAACGG TGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT