NegoA.17262.a

exodeoxyribonuclease V. alpha subunit

CENTER ID: NegoA.17262.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17262.a.B1.GE40062 full length 1 581
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17262.a.B1.PS37955 full length 1 581

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.1188
UniProt: B4RLU6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MELQTDEFAQ AAARAAIRFL ERYAGSGNEV LANCTERLFQ ALQNGHSFIR LSGDEADALS ALAPVVGTSA APLILEGRRL FLGRMWQLEY DLAAEIKRLA AAGTSAPDAA GARQNLAKWF QGAGSEGQRD AAALALLQFF MVITGGPGTG KTTTVAKLLA LICGENENLP HIALAAPTGK AAAHMARALH RAINGFDAPE AVRRHLLKLE GQTVHRLLKL SPPKMQAAFD HIRPLPFDVL IVDEASMLDT ALMLQLLKAV KTGARVILLG DENQLPSVGI GAVLSVLSQK TVLDGETHQR LAGFLPEHGF SVSANPPVLA QNTAHLSFSH RFGDNSGIGC LARAAVSGDE GAWALFDRFP DELEHSECSP NARVERLYRA HKAYWQAVKD GNIEAAYAGI SDIVVLAAWR QDAEDFNEAY CSYVRRKMNI PEHLAYFAGE PIMIRQNDYA LELFNGDIGL IMEDVGRQGS LAAYFADADG FKKVAVSCLP EFEPAFAMTV HKSQGSEYRE VWLLPPSDAP SDEGDDALSG LSKELLYTAI TRAREKFVFF GGKKTFCQAV NTVKVRQTAL GSMLERVFSQ E
NT Sequence
ATGGAACTGC AGACAGATGA ATTTGCCCAA GCGGCGGCAA GGGCGGCAAT CCGTTTTTTG GAACGCTATG CGGGTTCGGG CAACGAAGTG CTTGCAAACT GTACCGAACG CCTGTTTCAG GCATTGCAAA ACGGTCATTC GTTTATCCGT TTGAGCGGGG ACGAGGCTGA CGCGCTGTCG GCACTCGCAC CCGTGGTCGG AACATCCGCC GCGCCTTTGA TTTTGGAAGG CAGAAGGCTG TTTTTGGGCA GGATGTGGCA GTTGGAATAC GATTTGGCTG CCGAAATAAA ACGCTTGGCG GCAGCCGGCA CGTCCGCCCC CGACGCGGCA GGCGCGCGGC AAAACCTCGC AAAATGGTTT CAAGGCGCGG GAAGCGAAGG ACAGCGCGAT GCGGCCGCCT TGGCACTGTT GCAGTTTTTT ATGGTGATTA CCGGAGGGCC GGGAACGGGC AAAACGACAA CGGTTGCCAA ACTGCTGGCA CTGATTTGCG GTGAAAACGA AAATCTTCCC CATATCGCGC TTGCCGCACC GACGGGCAAA GCGGCGGCAC ATATGGCGCG CGCGCTGCAC CGTGCAATCA ACGGTTTTGA CGCGCCGGAG GCCGTCCGCC GCCATTTGCT CAAACTGGAA GGGCAAACCG TCCACCGACT GCTTAAGCTG AGCCCGCCCA AAATGCAGGC GGCGTTCGAC CATATCCGTC CGCTGCCGTT CGACGTGCTG ATTGTCGATG AAGCCTCCAT GTTGGATACC GCATTGATGC TGCAACTTTT GAAAGCGGTC AAAACCGGCG CGCGCGTGAT TCTGCTGGGC GATGAAAACC AGCTCCCGTC CGTCGGAATC GGGGCGGTGC TGTCGGTTTT GTCGCAAAAA ACCGTTTTGG ACGGAGAAAC GCACCAAAGG CTGGCCGGTT TCCTTCCGGA ACACGGTTTC AGCGTCAGCG CAAATCCGCC CGTGTTGGCG CAAAACACCG CCCATCTGTC GTTCAGCCAC CGCTTCGGCG ACAACAGCGG CATCGGCTGC CTTGCCCGTG CCGCCGTATC GGGCGATGAA GGGGCGTGGG CATTGTTTGA CCGGTTTCCG GACGAACTGG AACATTCGGA ATGCAGTCCG AATGCTCGGG TCGAAAGGTT GTACCGGGCG CACAAAGCCT ATTGGCAGGC GGTAAAAGAC GGCAATATCG AAGCCGCATA CGCAGGCATC TCGGATATCG TGGTTCTGGC AGCTTGGCGG CAGGATGCGG AAGACTTCAA CGAAGCCTAT TGCAGCTATG TACGCCGCAA AATGAACATA CCGGAACATT TGGCCTATTT CGCCGGAGAG CCGATTATGA TCAGACAGAA CGACTACGCG CTTGAGCTGT TCAACGGCGA CATCGGACTG ATTATGGAAG ATGTCGGACG GCAGGGCAGC CTTGCCGCCT ATTTTGCCGA TGCGGACGGA TTTAAAAAGG TAGCGGTAAG CTGCTTGCCC GAATTTGAAC CCGCATTCGC CATGACCGTC CACAAAAGCC AAGGTTCGGA ATATCGGGAA GTCTGGCTGC TGCCGCCTTC CGACGCACCT TCGGACGAAG GGGACGATGC ATTGTCCGGA TTGAGTAAGG AGCTGTTATA TACCGCCATT ACCCGCGCCA GGGAAAAATT CGTATTTTTC GGCGGCAAAA AGACATTTTG TCAGGCGGTA AACACCGTCA AAGTCCGTCA GACGGCGTTG GGCAGTATGC TTGAGCGGGT ATTTTCACAA GAATAA
Details for NegoA.17262.a.B1.GE40062
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME LQTDEFAQAA ARAAIRFLER YAGSGNEVLA NCTERLFQAL QNGHSFIRLS GDEADALSAL APVVGTSAAP LILEGRRLFL GRMWQLEYDL AAEIKRLAAA GTSAPDAAGA RQNLAKWFQG AGSEGQRDAA ALALLQFFMV ITGGPGTGKT TTVAKLLALI CGENENLPHI ALAAPTGKAA AHMARALHRS INGFDAPEAV RRHLLKLEGQ TVHRLLKLSP PKMQAAFDHI RPLPFDVLIV DEASMLDTAL MLQLXXXXXX GXRVILLGDE NQLPSVXIGA VLSVLSQKXV LDGETHQRLA XXXXXHGXXV SANPXXLAQN TAHLXXSHRF GDNSGIGCLA RAAVSGDEGA WALFDRFPDE LEHSECSPNA RVERLYRAHK AYWQAVKDGN IEAAYAGISD IVVLAAWRQD AEDFNEAYCS YVRRKMNIPE HLAYFAGEPI MIRQNDYALE LFNGDIGLIM EDVGRQGSLA AYFADADGFK KVAVSCLPEF EPAFAMTVHK SQGSEYREVW LLPPSDAPSD EGDDALSGLS KELLYTAITR AREKFVFFGG KKTFCQAVNT VKVRQTALGS XX
Validated NT Sequence
cannnntact gcccaacgcc gtctgacgga ctttgacggt gtttaccgcc tgacaaaatg tctttttgcc gccgaaaaat acgaattttt ccctggcgcg ggtaatggcg gtatataaca gctccttact caatccggac aatgcatcgt ccccttcgtc cgaaggtgcg tcggaaggcg gcagcagcca gacttcccga tattccgaac cttggctttt gtggacggtc atggcgaatg cgggttcaaa ttcgggcagg cagcttaccg ctaccttttt aaatccgtcc gcatcggcaa aataggcggc aaggctgccc tgccgtccga catcttccat aatcagtccg atgtcgccgt tgaacagctc aagcgcgtag tcgttctgtc tgatcataat cggctctccg gcgaaatagg ccaaatgttc cggtatgttc attttgcggc gtacatagct gcaataggct tcgttgaagt cttccgcatc ctgccgccaa gctgccagaa ccacgatatc cgagatgcct gcgtatgcgg cttcgatatt gccgtctttt accgcctgcc aataggcttt gtgcgcccgg tacaaccttt cgacccgagc attcggactg cattccgaat gttccagttc gtccggaaac cggtcaaaca atgcccacgc cccttcatcg cccgatacgg cggcacgggc aaggcagccg atgccgctgt tgtcgccgaa gcggtggctg nnnnacagat gggcggtgtt ttgcgccaac annnncggat ttgcgctgac gcnnaaaccg tgttnnnnna nnnnnncggc cagcctttgg tgcgtttctc cgtccaaaac nnntttttgc gacaaaacgg acagcaccgc cccgatnncg acggacggga gctggttttc atcgcccagc agaatcacgc gcncgccgnn nnnnancgnn nnnnnaagtt gcagcatcaa tgcggtatcc aacatggagg cttcatcgac aatcagcacg tcgaacggca gcggacggat atggtcgaac gccgcctgca ttttgggcgg gctcagctta agcagtcggt ggacggtttg cccttccagt ttgagcaaat ggcggcggac ggcctccggc gcgtcaaaac cgttgattga acggtgcagt gcgcgcgcca tatgtgccgc cgctttgccc gtcggtgcgg caagcgcgat atggggaaga ttttcgtttt caccgcaaat cagtgccagc agtttggcaa ccgttgtcgt tttgcccgtt cccggccctc cggtaatcac cataaaaaac tgcaacagtg ccaaggcggc cgcatcgcgc tgtccttcgc ttcccgcgcc ttgaaaccat tttgcgaggt tttgccgcgc gcctgccgcg tcgggggcgg acgtgccggc tgccgccaag cgttttattt cggcagccaa atcgtattcc aactgccaca tcctgcccaa aaacagcctt ctgccttcca aaatcaaagg cgcggcggat gttccgacca cgggtgcgag tgccgacagc gcgtcagcct cgtccccgct caaacggata aacgaatgac cgttttgcaa tgcctgaaac aggcgttcgg tacagtttgc aagcacttcg ttgcccgaac ccgcatagcg ttccaaaaaa cggattgccg cccttgccgc cgcttgggca aattcatctg tctgcagttc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHME LQTDEFAQAA ARAAIRFLER YAGSGNEVLA NCTERLFQAL QNGHSFIRLS GDEADALSAL APVVGTSAAP LILEGRRLFL GRMWQLEYDL AAEIKRLAAA GTSAPDAAGA RQNLAKWFQG AGSEGQRDAA ALALLQFFMV ITGGPGTGKT TTVAKLLALI CGENENLPHI ALAAPTGKAA AHMARALHRA INGFDAPEAV RRHLLKLEGQ TVHRLLKLSP PKMQAAFDHI RPLPFDVLIV DEASMLDTAL MLQLLKAVKT GARVILLGDE NQLPSVGIGA VLSVLSQKTV LDGETHQRLA GFLPEHGFSV SANPPVLAQN TAHLSFSHRF GDNSGIGCLA RAAVSGDEGA WALFDRFPDE LEHSECSPNA RVERLYRAHK AYWQAVKDGN IEAAYAGISD IVVLAAWRQD AEDFNEAYCS YVRRKMNIPE HLAYFAGEPI MIRQNDYALE LFNGDIGLIM EDVGRQGSLA AYFADADGFK KVAVSCLPEF EPAFAMTVHK SQGSEYREVW LLPPSDAPSD EGDDALSGLS KELLYTAITR AREKFVFFGG KKTFCQAVNT VKVRQTALGS MLERVFSQE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac tgcagacaga tgaatttgcc caagcggcgg caagggcggc aatccgtttt ttggaacgct atgcgggttc gggcaacgaa gtgcttgcaa actgtaccga acgcctgttt caggcattgc aaaacggtca ttcgtttatc cgtttgagcg gggacgaggc tgacgcgctg tcggcactcg cacccgtggt cggaacatcc gccgcgcctt tgattttgga aggcagaagg ctgtttttgg gcaggatgtg gcagttggaa tacgatttgg ctgccgaaat aaaacgcttg gcggcagccg gcacgtccgc ccccgacgcg gcaggcgcgc ggcaaaacct cgcaaaatgg tttcaaggcg cgggaagcga aggacagcgc gatgcggccg ccttggcact gttgcagttt tttatggtga ttaccggagg gccgggaacg ggcaaaacga caacggttgc caaactgctg gcactgattt gcggtgaaaa cgaaaatctt ccccatatcg cgcttgccgc accgacgggc aaagcggcgg cacatatggc gcgcgcgctg caccgtgcaa tcaacggttt tgacgcgccg gaggccgtcc gccgccattt gctcaaactg gaagggcaaa ccgtccaccg actgcttaag ctgagcccgc ccaaaatgca ggcggcgttc gaccatatcc gtccgctgcc gttcgacgtg ctgattgtcg atgaagcctc catgttggat accgcattga tgctgcaact tttgaaagcg gtcaaaaccg gcgcgcgcgt gattctgctg ggcgatgaaa accagctccc gtccgtcgga atcggggcgg tgctgtcggt tttgtcgcaa aaaaccgttt tggacggaga aacgcaccaa aggctggccg gtttccttcc ggaacacggt ttcagcgtca gcgcaaatcc gcccgtgttg gcgcaaaaca ccgcccatct gtcgttcagc caccgcttcg gcgacaacag cggcatcggc tgccttgccc gtgccgccgt atcgggcgat gaaggggcgt gggcattgtt tgaccggttt ccggacgaac tggaacattc ggaatgcagt ccgaatgctc gggtcgaaag gttgtaccgg gcgcacaaag cctattggca ggcggtaaaa gacggcaata tcgaagccgc atacgcaggc atctcggata tcgtggttct ggcagcttgg cggcaggatg cggaagactt caacgaagcc tattgcagct atgtacgccg caaaatgaac ataccggaac atttggccta tttcgccgga gagccgatta tgatcagaca gaacgactac gcgcttgagc tgttcaacgg cgacatcgga ctgattatgg aagatgtcgg acggcagggc agccttgccg cctattttgc cgatgcggac ggatttaaaa aggtagcggt aagctgcttg cccgaatttg aacccgcatt cgccatgacc gtccacaaaa gccaaggttc ggaatatcgg gaagtctggc tgctgccgcc ttccgacgca ccttcggacg aaggggacga tgcattgtcc ggattgagta aggagctgtt atataccgcc attacccgcg ccagggaaaa attcgtattt ttcggcggca aaaagacatt ttgtcaggcg gtaaacaccg tcaaagtccg tcagacggcg ttgggcagta tgcttgagcg ggtattttca caagaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NegoA.17262.a.B1.PS37955
PURIFICATION DATe: 6/28/2016
CONCENTRATION: 49mg/ml
OBSERVED MW: 68kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME LQTDEFAQAA ARAAIRFLER YAGSGNEVLA NCTERLFQAL QNGHSFIRLS GDEADALSAL APVVGTSAAP LILEGRRLFL GRMWQLEYDL AAEIKRLAAA GTSAPDAAGA RQNLAKWFQG AGSEGQRDAA ALALLQFFMV ITGGPGTGKT TTVAKLLALI CGENENLPHI ALAAPTGKAA AHMARALHRS INGFDAPEAV RRHLLKLEGQ TVHRLLKLSP PKMQAAFDHI RPLPFDVLIV DEASMLDTAL MLQLXXXXXX GXRVILLGDE NQLPSVXIGA VLSVLSQKXV LDGETHQRLA XXXXXHGXXV SANPXXLAQN TAHLXXSHRF GDNSGIGCLA RAAVSGDEGA WALFDRFPDE LEHSECSPNA RVERLYRAHK AYWQAVKDGN IEAAYAGISD IVVLAAWRQD AEDFNEAYCS YVRRKMNIPE HLAYFAGEPI MIRQNDYALE LFNGDIGLIM EDVGRQGSLA AYFADADGFK KVAVSCLPEF EPAFAMTVHK SQGSEYREVW LLPPSDAPSD EGDDALSGLS KELLYTAITR AREKFVFFGG KKTFCQAVNT VKVRQTALGS XX
Validated NT Sequence
cannnntact gcccaacgcc gtctgacgga ctttgacggt gtttaccgcc tgacaaaatg tctttttgcc gccgaaaaat acgaattttt ccctggcgcg ggtaatggcg gtatataaca gctccttact caatccggac aatgcatcgt ccccttcgtc cgaaggtgcg tcggaaggcg gcagcagcca gacttcccga tattccgaac cttggctttt gtggacggtc atggcgaatg cgggttcaaa ttcgggcagg cagcttaccg ctaccttttt aaatccgtcc gcatcggcaa aataggcggc aaggctgccc tgccgtccga catcttccat aatcagtccg atgtcgccgt tgaacagctc aagcgcgtag tcgttctgtc tgatcataat cggctctccg gcgaaatagg ccaaatgttc cggtatgttc attttgcggc gtacatagct gcaataggct tcgttgaagt cttccgcatc ctgccgccaa gctgccagaa ccacgatatc cgagatgcct gcgtatgcgg cttcgatatt gccgtctttt accgcctgcc aataggcttt gtgcgcccgg tacaaccttt cgacccgagc attcggactg cattccgaat gttccagttc gtccggaaac cggtcaaaca atgcccacgc cccttcatcg cccgatacgg cggcacgggc aaggcagccg atgccgctgt tgtcgccgaa gcggtggctg nnnnacagat gggcggtgtt ttgcgccaac annnncggat ttgcgctgac gcnnaaaccg tgttnnnnna nnnnnncggc cagcctttgg tgcgtttctc cgtccaaaac nnntttttgc gacaaaacgg acagcaccgc cccgatnncg acggacggga gctggttttc atcgcccagc agaatcacgc gcncgccgnn nnnnancgnn nnnnnaagtt gcagcatcaa tgcggtatcc aacatggagg cttcatcgac aatcagcacg tcgaacggca gcggacggat atggtcgaac gccgcctgca ttttgggcgg gctcagctta agcagtcggt ggacggtttg cccttccagt ttgagcaaat ggcggcggac ggcctccggc gcgtcaaaac cgttgattga acggtgcagt gcgcgcgcca tatgtgccgc cgctttgccc gtcggtgcgg caagcgcgat atggggaaga ttttcgtttt caccgcaaat cagtgccagc agtttggcaa ccgttgtcgt tttgcccgtt cccggccctc cggtaatcac cataaaaaac tgcaacagtg ccaaggcggc cgcatcgcgc tgtccttcgc ttcccgcgcc ttgaaaccat tttgcgaggt tttgccgcgc gcctgccgcg tcgggggcgg acgtgccggc tgccgccaag cgttttattt cggcagccaa atcgtattcc aactgccaca tcctgcccaa aaacagcctt ctgccttcca aaatcaaagg cgcggcggat gttccgacca cgggtgcgag tgccgacagc gcgtcagcct cgtccccgct caaacggata aacgaatgac cgttttgcaa tgcctgaaac aggcgttcgg tacagtttgc aagcacttcg ttgcccgaac ccgcatagcg ttccaaaaaa cggattgccg cccttgccgc cgcttgggca aattcatctg tctgcagttc catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHME LQTDEFAQAA ARAAIRFLER YAGSGNEVLA NCTERLFQAL QNGHSFIRLS GDEADALSAL APVVGTSAAP LILEGRRLFL GRMWQLEYDL AAEIKRLAAA GTSAPDAAGA RQNLAKWFQG AGSEGQRDAA ALALLQFFMV ITGGPGTGKT TTVAKLLALI CGENENLPHI ALAAPTGKAA AHMARALHRA INGFDAPEAV RRHLLKLEGQ TVHRLLKLSP PKMQAAFDHI RPLPFDVLIV DEASMLDTAL MLQLLKAVKT GARVILLGDE NQLPSVGIGA VLSVLSQKTV LDGETHQRLA GFLPEHGFSV SANPPVLAQN TAHLSFSHRF GDNSGIGCLA RAAVSGDEGA WALFDRFPDE LEHSECSPNA RVERLYRAHK AYWQAVKDGN IEAAYAGISD IVVLAAWRQD AEDFNEAYCS YVRRKMNIPE HLAYFAGEPI MIRQNDYALE LFNGDIGLIM EDVGRQGSLA AYFADADGFK KVAVSCLPEF EPAFAMTVHK SQGSEYREVW LLPPSDAPSD EGDDALSGLS KELLYTAITR AREKFVFFGG KKTFCQAVNT VKVRQTALGS MLERVFSQE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAC TGCAGACAGA TGAATTTGCC CAAGCGGCGG CAAGGGCGGC AATCCGTTTT TTGGAACGCT ATGCGGGTTC GGGCAACGAA GTGCTTGCAA ACTGTACCGA ACGCCTGTTT CAGGCATTGC AAAACGGTCA TTCGTTTATC CGTTTGAGCG GGGACGAGGC TGACGCGCTG TCGGCACTCG CACCCGTGGT CGGAACATCC GCCGCGCCTT TGATTTTGGA AGGCAGAAGG CTGTTTTTGG GCAGGATGTG GCAGTTGGAA TACGATTTGG CTGCCGAAAT AAAACGCTTG GCGGCAGCCG GCACGTCCGC CCCCGACGCG GCAGGCGCGC GGCAAAACCT CGCAAAATGG TTTCAAGGCG CGGGAAGCGA AGGACAGCGC GATGCGGCCG CCTTGGCACT GTTGCAGTTT TTTATGGTGA TTACCGGAGG GCCGGGAACG GGCAAAACGA CAACGGTTGC CAAACTGCTG GCACTGATTT GCGGTGAAAA CGAAAATCTT CCCCATATCG CGCTTGCCGC ACCGACGGGC AAAGCGGCGG CACATATGGC GCGCGCGCTG CACCGTGCAA TCAACGGTTT TGACGCGCCG GAGGCCGTCC GCCGCCATTT GCTCAAACTG GAAGGGCAAA CCGTCCACCG ACTGCTTAAG CTGAGCCCGC CCAAAATGCA GGCGGCGTTC GACCATATCC GTCCGCTGCC GTTCGACGTG CTGATTGTCG ATGAAGCCTC CATGTTGGAT ACCGCATTGA TGCTGCAACT TTTGAAAGCG GTCAAAACCG GCGCGCGCGT GATTCTGCTG GGCGATGAAA ACCAGCTCCC GTCCGTCGGA ATCGGGGCGG TGCTGTCGGT TTTGTCGCAA AAAACCGTTT TGGACGGAGA AACGCACCAA AGGCTGGCCG GTTTCCTTCC GGAACACGGT TTCAGCGTCA GCGCAAATCC GCCCGTGTTG GCGCAAAACA CCGCCCATCT GTCGTTCAGC CACCGCTTCG GCGACAACAG CGGCATCGGC TGCCTTGCCC GTGCCGCCGT ATCGGGCGAT GAAGGGGCGT GGGCATTGTT TGACCGGTTT CCGGACGAAC TGGAACATTC GGAATGCAGT CCGAATGCTC GGGTCGAAAG GTTGTACCGG GCGCACAAAG CCTATTGGCA GGCGGTAAAA GACGGCAATA TCGAAGCCGC ATACGCAGGC ATCTCGGATA TCGTGGTTCT GGCAGCTTGG CGGCAGGATG CGGAAGACTT CAACGAAGCC TATTGCAGCT ATGTACGCCG CAAAATGAAC ATACCGGAAC ATTTGGCCTA TTTCGCCGGA GAGCCGATTA TGATCAGACA GAACGACTAC GCGCTTGAGC TGTTCAACGG CGACATCGGA CTGATTATGG AAGATGTCGG ACGGCAGGGC AGCCTTGCCG CCTATTTTGC CGATGCGGAC GGATTTAAAA AGGTAGCGGT AAGCTGCTTG CCCGAATTTG AACCCGCATT CGCCATGACC GTCCACAAAA GCCAAGGTTC GGAATATCGG GAAGTCTGGC TGCTGCCGCC TTCCGACGCA CCTTCGGACG AAGGGGACGA TGCATTGTCC GGATTGAGTA AGGAGCTGTT ATATACCGCC ATTACCCGCG CCAGGGAAAA ATTCGTATTT TTCGGCGGCA AAAAGACATT TTGTCAGGCG GTAAACACCG TCAAAGTCCG TCAGACGGCG TTGGGCAGTA TGCTTGAGCG GGTATTTTCA CAAGAATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T