NegoA.17241.a

1-deoxy-D-xylulose-5-phosphate synthase

CENTER ID: NegoA.17241.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17241.a.B1.GE40061 full length 1 637
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17241.a.B1.PS37961 full length 1 637

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.55
UniProt: B4RNW6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPSPLLDLI DSPQDLRRLD KKQLPRLAGE LRAFLLESVG QTGGHFASNL GAVELTIALH YVYDTPEDKL VWDVGHQSYP HKILTGRKNQ MHTMRQYGGL AGFPKRCESE YDAFGVGHSS TSIGAALGMA AADKLLGGDR RSVAIIGDGA MTAGQAFEAL NCAGDMDVDL LVVLNDNEMS ISPNVGALPK YLASNVVRDM HGLLSTVKAQ TGKVLDKIPG AMEFAQKVEH KIKTLAEEAE HAKQSLSLFE NFGFRYTGPV DGHNVENLVD VLKDLRSRKG PQLLHVITKK GNGYKLAEND PVKYHAVANL PKEGGAQMPS EKEPKPAAKP TYTQVFGKWL CDRAAADSRL VAITPAMREG SGLVEFEQRF PDRYFDVGIA EQHAVTFAGG LACEGMKPVV AIYSTFLQRA YDQLVHDIAL QNLPVLFAVD RAGIVGADGP THAGLYDLSF LRCVPNMIVA APSDENECRL LLSTCYQADA PAAVRYPRGT GTGAPVSDGM ETVEIGKGII RREGEKTAFI AFGSMVAPAL AVAEKLNATV ADMRFVKPID EELIVRLARS HDRIVTLEEN AEQGGAGGAV LEVLAKHGIC KPVLLLGVAD TVTEHGDPKK LLDDLGLSAE AVERRVREWL PDRDAAN
NT Sequence
ATGAACCCAA GCCCCCTACT CGACCTGATT GACAGCCCGC AAGATTTGCG CCGCCTGGAC AAAAAACAGC TGCCGCGCCT TGCCGGCGAG TTGCGCGCCT TTCTGCTGGA ATCTGTCGGG CAGACCGGCG GGCATTTCGC CAGCAATCTG GGTGCGGTCG AACTGACCAT CGCCCTGCAC TATGTGTACG ACACGCCCGA AGACAAGCTG GTGTGGGATG TCGGACACCA AAGCTACCCG CACAAAATCC TGACAGGCAG GAAAAACCAG ATGCACACCA TGCGCCAATA CGGCGGTTTG GCGGGTTTTC CGAAACGTTG CGAGTCCGAG TACGACGCGT TCGGCGTGGG GCATTCCTCC ACCTCCATCG GCGCGGCTTT GGGCATGGCG GCGGCGGACA AACTCTTGGG CGGCGACCGC CGCAGCGTCG CCATCATCGG CGACGGCGCG ATGACGGCGG GGCAGGCGTT TGAAGCCTTG AATTGCGCGG GCGATATGGA TGTGGATTTG CTGGTCGTCC TCAACGACAA CGAAATGTCG ATTTCCCCCA ACGTCGGCGC GTTGCCCAAA TATCTTGCCA GCAACGTCGT GCGCGATATG CACGGACTGT TGAGTACCGT CAAAGCGCAA ACGGGCAAGG TATTAGACAA AATACCCGGC GCGATGGAGT TTGCCCAAAA AGTCGAACAC AAAATCAAAA CCCTTGCCGA AGAAGCCGAA CACGCCAAAC AGTCGCTGTC GCTGTTTGAA AATTTCGGCT TCCGCTACAC CGGCCCCGTG GACGGACACA ACGTCGAGAA TCTGGTGGAC GTATTGAAAG ACTTGCGCAG CCGCAAAGGC CCTCAGTTGC TGCACGTCAT CACCAAAAAG GGCAACGGCT ACAAACTCGC CGAAAACGAC CCCGTCAAAT ACCACGCCGT CGCCAACCTG CCTAAAGAAG GCGGGGCGCA AATGCCGTCT GAAAAAGAAC CCAAGCCCGC CGCCAAACCG ACCTATACCC AAGTATTCGG CAAATGGCTG TGCGACCGGG CGGCGGCAGA TTCCCGACTG GTTGCGATTA CCCCCGCCAT GCGCGAGGGC AGCGGACTGG TGGAGTTTGA ACAACGATTC CCCGACCGCT ATTTCGATGT CGGCATCGCC GAGCAGCACG CCGTTACCTT TGCCGGCGGT TTGGCGTGCG AAGGCATGAA GCCCGTCGTG GCGATTTATT CCACCTTTTT ACAACGCGCC TACGACCAAC TGGTGCACGA CATCGCCCTG CAAAACCTGC CCGTTTTGTT TGCCGTCGAC CGTGCGGGCA TCGTCGGCGC GGACGGTCCG ACCCATGCCG GCTTGTACGA TTTGAGCTTC TTGCGCTGTG TGCCGAACAT GATTGTTGCC GCGCCGAGCG ATGAAAACGA ATGCCGCCTG CTGCTTTCGA CCTGCTATCA GGCGGATGCG CCCGCCGCCG TCCGCTATCC GCGCGGCACG GGTACGGGCG CGCCGGTTTC AGACGGCATG GAAACCGTGG AAATCGGCAA GGGCATTATC CGCCGCGAAG GTGAGAAAAC CGCCTTCATT GCCTTCGGCA GTATGGTCGC CCCCGCATTG GCGGTTGCCG AAAAACTGAA CGCCACCGTC GCCGATATGC GCTTCGTCAA ACCGATAGAC GAAGAGTTGA TTGTCCGCCT TGCCCGAAGC CACGACCGCA TCGTTACCCT TGAAGAAAAC GCCGAACAGG GCGGCGCAGG CGGCGCGGTC TTGGAAGTGT TGGCGAAACA CGGCATCTGC AAACCCGTTT TGCTTTTGGG CGTTGCCGAT ACCGTAACCG AACACGGCGA TCCGAAAAAA CTTTTGGACG ATTTGGGTTT GAGTGCCGAA GCGGTGGAAC GCCGGGTGCG CGAGTGGCTG CCGGACCGTG ATGCGGCAAA TTAA
Details for NegoA.17241.a.B1.GE40061
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 70kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 69
PERCENT COVERAGE: 62
Validated AA Sequence
MAHHHHHHMN PSPLLHLIDS PQDLRRLDKK QLPRLAGELR AFLLESVGQT GGHFASNLGA VELTIALHYV YDTPEDKLVW DVGHQSYPHK ILTGRKNQMH TMRQYGGLAG FPKRCESEYD AFGVGHSSTS IGAALGMAAA DKLLGGDRRS VAIIGDGAMT AGQAFEALNC AGDMDVDLLV VLNDNEMSIS PNVGALPKYL ASNVVRDMHG LLSTVKAQTG KVLDKIPGAM EFAQKVEHKI KTLAXXAEHA KQXXSLFENF GFRYTGPVDG XXVXXXXXXX XXXXXXXGPX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXAXXXXX XXXXXXXXXR XXXXXXAXXX XXXRRXXXXX XXLXXXCARA ADXWSLNNDS PTAISMSASP SSTPLPLPAV WRAKA
Validated NT Sequence
cactcgcgca cccggcgttc caccgcttcg gcactcaaac ccaaatcgtc caaaagtttt ttcggatcgc cgtgttcggt tacggtatcg gcaacgccca aaagcaaaac gggtttgcag atgccgtgtt tcgccaacac ttccaagacc gcgccgcctg cgccgccctg ttcggcgttt tcttcaaggg taacgatgcg gtcgtggctt cgggcaaggc ggacaatcaa ctcttcgtct atcggtttga cgaagcgcat atcggcgacg gtggcgttca gtttttcggc aaccgccaat gcggtggcga ccatactgcc gaaggcaatg aaggcggttt tctcaccttc gcggcggata atgcccttgc cgatttccac ggtttccatg ccgtctgaaa ccggcgcgcc cgtacccgtg ccgcgcggat agcggacggc ggcgggcgca tccgcctgat agcaggtcga aagcagcagg cggcattcgt tttcatcgct cggcgcggca acaatcatgt tcggcacaca gcgcaagaag ctcaaatcgt acaagccggc atgggtcgga ccgtccgcgc cgacgatgcc cgcacggtcg acggcaaaca aaacgggcag gttttgcagg gcgatgtcgt gcaccagttg gtcgtaggcg cgttgtaaaa aggtggaata aatcgccacg acgggcttca tgccttcgca cgccaaaccg ccggcaaagg taacggcgtg ctgctcggcg atgccgacat cgaaatagcg gtcggggaat cgttgttcaa actccancag tccgctgccc tcgcgcatnn nnnnnntaat nnnannnagn cgnnnnnntg ccgccgncng nnnnnnnnnn nntttgcnnn nnnnnnnnnn nnnnntcgnn nnnnnnnnnn nnnnnnnnnn nnnnttnnnn nnnnnnnnnn ngcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntnnnnn nnnnnnnnnn ngnnnnnnag ggcctnnnnn nnnnnnnann nnnttcannn nnnnnnnnng atnnncnacg tnnngtccgt ccacggggcc ggtgtagcgg aagccgaaat tttcaaacag cgacannnac tgtttggcgt gttcggcnnc ntcggcaagg gttttgattt tgtgttcgac tttttgggca aactccatcg cgccgggtat tttgtctaat accttgcccg tttgcgcttt gacggtactc aacagtccgt gcatatcgcg cacgacgttg ctggcaagat atttgggcaa cgcgccgacg ttgggggaaa tcgacatttc gttgtcgttg aggacgacca gcaaatccac atccatatcg cccgcgcaat tcaaggcttc aaacgcctgc cccgccgtca tcgcgccgtc tccgatgatg gcgacgctgc ggcggtcgcc gcccaagagt ttgtccgccg ccgccatgcc caaagccgcg ccgatggagg tggaggaatg ccccacgccg aacgcgtcgt actcggactc gcaacgtttc ggaaaacccg ccaaaccgcc gtattggcgc atggtgtgca tctggttttt cctgcctgtc aggattttgt gcgggtagct ttggtgtccg acatcccaca ccagcttgtc ttcgggcgtg tcgtacacat agtgcagggc gatggtcagt tcgaccgcac ccagattgct ggcgaaatgc ccgccggtct gcccgacaga ttccagcaga aaggcgcgca actcgccggc aaggcgcggc agctgttttt tgtccaggcg gcgcaaatct tgcgggctgt caatcaggtg gagtaggggg cttgggttca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMN PSPLLDLIDS PQDLRRLDKK QLPRLAGELR AFLLESVGQT GGHFASNLGA VELTIALHYV YDTPEDKLVW DVGHQSYPHK ILTGRKNQMH TMRQYGGLAG FPKRCESEYD AFGVGHSSTS IGAALGMAAA DKLLGGDRRS VAIIGDGAMT AGQAFEALNC AGDMDVDLLV VLNDNEMSIS PNVGALPKYL ASNVVRDMHG LLSTVKAQTG KVLDKIPGAM EFAQKVEHKI KTLAEEAEHA KQSLSLFENF GFRYTGPVDG HNVENLVDVL KDLRSRKGPQ LLHVITKKGN GYKLAENDPV KYHAVANLPK EGGAQMPSEK EPKPAAKPTY TQVFGKWLCD RAAADSRLVA ITPAMREGSG LVEFEQRFPD RYFDVGIAEQ HAVTFAGGLA CEGMKPVVAI YSTFLQRAYD QLVHDIALQN LPVLFAVDRA GIVGADGPTH AGLYDLSFLR CVPNMIVAAP SDENECRLLL STCYQADAPA AVRYPRGTGT GAPVSDGMET VEIGKGIIRR EGEKTAFIAF GSMVAPALAV AEKLNATVAD MRFVKPIDEE LIVRLARSHD RIVTLEENAE QGGAGGAVLE VLAKHGICKP VLLLGVADTV TEHGDPKKLL DDLGLSAEAV ERRVREWLPD RDAAN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc caagccccct actcgacctg attgacagcc cgcaagattt gcgccgcctg gacaaaaaac agctgccgcg ccttgccggc gagttgcgcg cctttctgct ggaatctgtc gggcagaccg gcgggcattt cgccagcaat ctgggtgcgg tcgaactgac catcgccctg cactatgtgt acgacacgcc cgaagacaag ctggtgtggg atgtcggaca ccaaagctac ccgcacaaaa tcctgacagg caggaaaaac cagatgcaca ccatgcgcca atacggcggt ttggcgggtt ttccgaaacg ttgcgagtcc gagtacgacg cgttcggcgt ggggcattcc tccacctcca tcggcgcggc tttgggcatg gcggcggcgg acaaactctt gggcggcgac cgccgcagcg tcgccatcat cggcgacggc gcgatgacgg cggggcaggc gtttgaagcc ttgaattgcg cgggcgatat ggatgtggat ttgctggtcg tcctcaacga caacgaaatg tcgatttccc ccaacgtcgg cgcgttgccc aaatatcttg ccagcaacgt cgtgcgcgat atgcacggac tgttgagtac cgtcaaagcg caaacgggca aggtattaga caaaataccc ggcgcgatgg agtttgccca aaaagtcgaa cacaaaatca aaacccttgc cgaagaagcc gaacacgcca aacagtcgct gtcgctgttt gaaaatttcg gcttccgcta caccggcccc gtggacggac acaacgtcga gaatctggtg gacgtattga aagacttgcg cagccgcaaa ggccctcagt tgctgcacgt catcaccaaa aagggcaacg gctacaaact cgccgaaaac gaccccgtca aataccacgc cgtcgccaac ctgcctaaag aaggcggggc gcaaatgccg tctgaaaaag aacccaagcc cgccgccaaa ccgacctata cccaagtatt cggcaaatgg ctgtgcgacc gggcggcggc agattcccga ctggttgcga ttacccccgc catgcgcgag ggcagcggac tggtggagtt tgaacaacga ttccccgacc gctatttcga tgtcggcatc gccgagcagc acgccgttac ctttgccggc ggtttggcgt gcgaaggcat gaagcccgtc gtggcgattt attccacctt tttacaacgc gcctacgacc aactggtgca cgacatcgcc ctgcaaaacc tgcccgtttt gtttgccgtc gaccgtgcgg gcatcgtcgg cgcggacggt ccgacccatg ccggcttgta cgatttgagc ttcttgcgct gtgtgccgaa catgattgtt gccgcgccga gcgatgaaaa cgaatgccgc ctgctgcttt cgacctgcta tcaggcggat gcgcccgccg ccgtccgcta tccgcgcggc acgggtacgg gcgcgccggt ttcagacggc atggaaaccg tggaaatcgg caagggcatt atccgccgcg aaggtgagaa aaccgccttc attgccttcg gcagtatggt cgcccccgca ttggcggttg ccgaaaaact gaacgccacc gtcgccgata tgcgcttcgt caaaccgata gacgaagagt tgattgtccg ccttgcccga agccacgacc gcatcgttac ccttgaagaa aacgccgaac agggcggcgc aggcggcgcg gtcttggaag tgttggcgaa acacggcatc tgcaaacccg ttttgctttt gggcgttgcc gataccgtaa ccgaacacgg cgatccgaaa aaacttttgg acgatttggg tttgagtgcc gaagcggtgg aacgccgggt gcgcgagtgg ctgccggacc gtgatgcggc aaattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for NegoA.17241.a.B1.PS37961
PURIFICATION DATe: 6/29/2016
CONCENTRATION: 59.27mg/ml
OBSERVED MW: 74kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 69
PERCENT COVERAGE: 62
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN PSPLLHLIDS PQDLRRLDKK QLPRLAGELR AFLLESVGQT GGHFASNLGA VELTIALHYV YDTPEDKLVW DVGHQSYPHK ILTGRKNQMH TMRQYGGLAG FPKRCESEYD AFGVGHSSTS IGAALGMAAA DKLLGGDRRS VAIIGDGAMT AGQAFEALNC AGDMDVDLLV VLNDNEMSIS PNVGALPKYL ASNVVRDMHG LLSTVKAQTG KVLDKIPGAM EFAQKVEHKI KTLAXXAEHA KQXXSLFENF GFRYTGPVDG XXVXXXXXXX XXXXXXXGPX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXAXXXXX XXXXXXXXXR XXXXXXAXXX XXXRRXXXXX XXLXXXCARA ADXWSLNNDS PTAISMSASP SSTPLPLPAV WRAKA
Validated NT Sequence
cactcgcgca cccggcgttc caccgcttcg gcactcaaac ccaaatcgtc caaaagtttt ttcggatcgc cgtgttcggt tacggtatcg gcaacgccca aaagcaaaac gggtttgcag atgccgtgtt tcgccaacac ttccaagacc gcgccgcctg cgccgccctg ttcggcgttt tcttcaaggg taacgatgcg gtcgtggctt cgggcaaggc ggacaatcaa ctcttcgtct atcggtttga cgaagcgcat atcggcgacg gtggcgttca gtttttcggc aaccgccaat gcggtggcga ccatactgcc gaaggcaatg aaggcggttt tctcaccttc gcggcggata atgcccttgc cgatttccac ggtttccatg ccgtctgaaa ccggcgcgcc cgtacccgtg ccgcgcggat agcggacggc ggcgggcgca tccgcctgat agcaggtcga aagcagcagg cggcattcgt tttcatcgct cggcgcggca acaatcatgt tcggcacaca gcgcaagaag ctcaaatcgt acaagccggc atgggtcgga ccgtccgcgc cgacgatgcc cgcacggtcg acggcaaaca aaacgggcag gttttgcagg gcgatgtcgt gcaccagttg gtcgtaggcg cgttgtaaaa aggtggaata aatcgccacg acgggcttca tgccttcgca cgccaaaccg ccggcaaagg taacggcgtg ctgctcggcg atgccgacat cgaaatagcg gtcggggaat cgttgttcaa actccancag tccgctgccc tcgcgcatnn nnnnnntaat nnnannnagn cgnnnnnntg ccgccgncng nnnnnnnnnn nntttgcnnn nnnnnnnnnn nnnnntcgnn nnnnnnnnnn nnnnnnnnnn nnnnttnnnn nnnnnnnnnn ngcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntnnnnn nnnnnnnnnn ngnnnnnnag ggcctnnnnn nnnnnnnann nnnttcannn nnnnnnnnng atnnncnacg tnnngtccgt ccacggggcc ggtgtagcgg aagccgaaat tttcaaacag cgacannnac tgtttggcgt gttcggcnnc ntcggcaagg gttttgattt tgtgttcgac tttttgggca aactccatcg cgccgggtat tttgtctaat accttgcccg tttgcgcttt gacggtactc aacagtccgt gcatatcgcg cacgacgttg ctggcaagat atttgggcaa cgcgccgacg ttgggggaaa tcgacatttc gttgtcgttg aggacgacca gcaaatccac atccatatcg cccgcgcaat tcaaggcttc aaacgcctgc cccgccgtca tcgcgccgtc tccgatgatg gcgacgctgc ggcggtcgcc gcccaagagt ttgtccgccg ccgccatgcc caaagccgcg ccgatggagg tggaggaatg ccccacgccg aacgcgtcgt actcggactc gcaacgtttc ggaaaacccg ccaaaccgcc gtattggcgc atggtgtgca tctggttttt cctgcctgtc aggattttgt gcgggtagct ttggtgtccg acatcccaca ccagcttgtc ttcgggcgtg tcgtacacat agtgcagggc gatggtcagt tcgaccgcac ccagattgct ggcgaaatgc ccgccggtct gcccgacaga ttccagcaga aaggcgcgca actcgccggc aaggcgcggc agctgttttt tgtccaggcg gcgcaaatct tgcgggctgt caatcaggtg gagtaggggg cttgggttca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMN PSPLLDLIDS PQDLRRLDKK QLPRLAGELR AFLLESVGQT GGHFASNLGA VELTIALHYV YDTPEDKLVW DVGHQSYPHK ILTGRKNQMH TMRQYGGLAG FPKRCESEYD AFGVGHSSTS IGAALGMAAA DKLLGGDRRS VAIIGDGAMT AGQAFEALNC AGDMDVDLLV VLNDNEMSIS PNVGALPKYL ASNVVRDMHG LLSTVKAQTG KVLDKIPGAM EFAQKVEHKI KTLAEEAEHA KQSLSLFENF GFRYTGPVDG HNVENLVDVL KDLRSRKGPQ LLHVITKKGN GYKLAENDPV KYHAVANLPK EGGAQMPSEK EPKPAAKPTY TQVFGKWLCD RAAADSRLVA ITPAMREGSG LVEFEQRFPD RYFDVGIAEQ HAVTFAGGLA CEGMKPVVAI YSTFLQRAYD QLVHDIALQN LPVLFAVDRA GIVGADGPTH AGLYDLSFLR CVPNMIVAAP SDENECRLLL STCYQADAPA AVRYPRGTGT GAPVSDGMET VEIGKGIIRR EGEKTAFIAF GSMVAPALAV AEKLNATVAD MRFVKPIDEE LIVRLARSHD RIVTLEENAE QGGAGGAVLE VLAKHGICKP VLLLGVADTV TEHGDPKKLL DDLGLSAEAV ERRVREWLPD RDAAN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC CAAGCCCCCT ACTCGACCTG ATTGACAGCC CGCAAGATTT GCGCCGCCTG GACAAAAAAC AGCTGCCGCG CCTTGCCGGC GAGTTGCGCG CCTTTCTGCT GGAATCTGTC GGGCAGACCG GCGGGCATTT CGCCAGCAAT CTGGGTGCGG TCGAACTGAC CATCGCCCTG CACTATGTGT ACGACACGCC CGAAGACAAG CTGGTGTGGG ATGTCGGACA CCAAAGCTAC CCGCACAAAA TCCTGACAGG CAGGAAAAAC CAGATGCACA CCATGCGCCA ATACGGCGGT TTGGCGGGTT TTCCGAAACG TTGCGAGTCC GAGTACGACG CGTTCGGCGT GGGGCATTCC TCCACCTCCA TCGGCGCGGC TTTGGGCATG GCGGCGGCGG ACAAACTCTT GGGCGGCGAC CGCCGCAGCG TCGCCATCAT CGGCGACGGC GCGATGACGG CGGGGCAGGC GTTTGAAGCC TTGAATTGCG CGGGCGATAT GGATGTGGAT TTGCTGGTCG TCCTCAACGA CAACGAAATG TCGATTTCCC CCAACGTCGG CGCGTTGCCC AAATATCTTG CCAGCAACGT CGTGCGCGAT ATGCACGGAC TGTTGAGTAC CGTCAAAGCG CAAACGGGCA AGGTATTAGA CAAAATACCC GGCGCGATGG AGTTTGCCCA AAAAGTCGAA CACAAAATCA AAACCCTTGC CGAAGAAGCC GAACACGCCA AACAGTCGCT GTCGCTGTTT GAAAATTTCG GCTTCCGCTA CACCGGCCCC GTGGACGGAC ACAACGTCGA GAATCTGGTG GACGTATTGA AAGACTTGCG CAGCCGCAAA GGCCCTCAGT TGCTGCACGT CATCACCAAA AAGGGCAACG GCTACAAACT CGCCGAAAAC GACCCCGTCA AATACCACGC CGTCGCCAAC CTGCCTAAAG AAGGCGGGGC GCAAATGCCG TCTGAAAAAG AACCCAAGCC CGCCGCCAAA CCGACCTATA CCCAAGTATT CGGCAAATGG CTGTGCGACC GGGCGGCGGC AGATTCCCGA CTGGTTGCGA TTACCCCCGC CATGCGCGAG GGCAGCGGAC TGGTGGAGTT TGAACAACGA TTCCCCGACC GCTATTTCGA TGTCGGCATC GCCGAGCAGC ACGCCGTTAC CTTTGCCGGC GGTTTGGCGT GCGAAGGCAT GAAGCCCGTC GTGGCGATTT ATTCCACCTT TTTACAACGC GCCTACGACC AACTGGTGCA CGACATCGCC CTGCAAAACC TGCCCGTTTT GTTTGCCGTC GACCGTGCGG GCATCGTCGG CGCGGACGGT CCGACCCATG CCGGCTTGTA CGATTTGAGC TTCTTGCGCT GTGTGCCGAA CATGATTGTT GCCGCGCCGA GCGATGAAAA CGAATGCCGC CTGCTGCTTT CGACCTGCTA TCAGGCGGAT GCGCCCGCCG CCGTCCGCTA TCCGCGCGGC ACGGGTACGG GCGCGCCGGT TTCAGACGGC ATGGAAACCG TGGAAATCGG CAAGGGCATT ATCCGCCGCG AAGGTGAGAA AACCGCCTTC ATTGCCTTCG GCAGTATGGT CGCCCCCGCA TTGGCGGTTG CCGAAAAACT GAACGCCACC GTCGCCGATA TGCGCTTCGT CAAACCGATA GACGAAGAGT TGATTGTCCG CCTTGCCCGA AGCCACGACC GCATCGTTAC CCTTGAAGAA AACGCCGAAC AGGGCGGCGC AGGCGGCGCG GTCTTGGAAG TGTTGGCGAA ACACGGCATC TGCAAACCCG TTTTGCTTTT GGGCGTTGCC GATACCGTAA CCGAACACGG CGATCCGAAA AAACTTTTGG ACGATTTGGG TTTGAGTGCC GAAGCGGTGG AACGCCGGGT GCGCGAGTGG CTGCCGGACC GTGATGCGGC AAATTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT