SSGCID: Target Page

NegoA.17208.a

TIGR02943 family RNA polymerase sigma-70 factor

CENTER ID:	NegoA.17208.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.17208.a.B1.GE40060	full length		1	195

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	B4RPN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPLPDLTDAE LMESRKLLLH FARLQLPDHP DLAEDLVQET LLSAYSAGDS FQGRALVNSW LFAILKNKII DALRQIGRQR KVFTALDDEL LDEAFESHFS QNGHWTPEGQ PQHWNTPEKS LNNNEFQKIL QSCLYNLPEN TARVFTLKEI LGFSSNEIQQ MCGISTSNYH TIMHRARESL RQCLQIKWFN QENPK

NT Sequence

ATGCCGCTAC CCGACCTGAC CGATGCCGAA TTAATGGAGT CGCGCAAACT GCTTCTGCAT TTTGCGCGAC TCCAGTTGCC CGACCACCCT GATTTGGCTG AAGATTTAGT GCAGGAAACA TTGCTGTCCG CATACAGCGC AGGCGACAGT TTTCAAGGCA GGGCACTTGT CAACAGCTGG CTTTTTGCCA TATTGAAAAA CAAAATTATT GACGCATTAC GTCAAATCGG GAGGCAGAGG AAAGTCTTTA CCGCACTGGA TGACGAGCTG CTGGATGAAG CATTTGAAAG CCATTTTTCC CAAAACGGGC ATTGGACGCC GGAAGGGCAG CCGCAACATT GGAACACTCC GGAAAAATCA TTAAATAACA ACGAATTCCA AAAAATTCTG CAAAGCTGCC TATACAACCT GCCTGAAAAC ACCGCACGGG TATTCACCCT GAAGGAAATA CTCGGTTTTT CATCCAACGA AATACAACAA ATGTGCGGTA TCAGCACGTC CAACTACCAC ACCATTATGC ACCGCGCCCG AGAATCATTG CGCCAATGCC TGCAAATCAA ATGGTTCAAC CAAGAAAACC CGAAGTAA

Details for NegoA.17208.a.B1.GE40060

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	24kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMP LPDLTDAELM ESRKLLLHFA RLQLPDHPDL AEDLVQETLL SAYSAGDSFQ GRALVNSWLF AILKNKIIDA LRQIGRQRKV FTALDDELLD EAFESHFSQN GHWTPEGQPQ HWNTPEKSLN NNEFQKILQS CLYNLPENTA RVFTLKEILG FSSNEIQQMC GISTSNYHTI MHRARESLRQ CLQIKWFNQE NPK

Validated NT Sequence

atcttactca cttacttcgg gttttcttgg ttgaaccatt tgatttgcag gcattggcgc aatgattctc gggcgcggtg cataatggtg tggtagttgg acgtgctgat accgcacatt tgttgtattt cgttggatga aaaaccgagt atttccttca gggtgaatac ccgtgcggtg ttttcaggca ggttgtatag gcagctttgc agaatttttt ggaattcgtt gttatttaat gatttttccg gagtgttcca atgttgcggc tgcccttccg gcgtccaatg cccgttttgg gaaaaatggc tttcaaatgc ttcatccagc agctcgtcat ccagtgcggt aaagactttc ctctgcctcc cgatttgacg taatgcgtca ataattttgt ttttcaatat ggcaaaaagc cagctgttga caagtgccct gccttgaaaa ctgtcgcctg cgctgtatgc ggacagcaat gtttcctgca ctaaatcttc agccaaatca gggtggtcgg gcaactggag ccgcgcaaaa tgcagaagca gtttgcgcga ctccattaat tcggcatcgg tcaggtcggg tagcggcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc tacccgacct gaccgatgcc gaattaatgg agtcgcgcaa actgcttctg cattttgcgc gactccagtt gcccgaccac cctgatttgg ctgaagattt agtgcaggaa acattgctgt ccgcatacag cgcaggcgac agttttcaag gcagggcact tgtcaacagc tggctttttg ccatattgaa aaacaaaatt attgacgcat tacgtcaaat cgggaggcag aggaaagtct ttaccgcact ggatgacgag ctgctggatg aagcatttga aagccatttt tcccaaaacg ggcattggac gccggaaggg cagccgcaac attggaacac tccggaaaaa tcattaaata acaacgaatt ccaaaaaatt ctgcaaagct gcctatacaa cctgcctgaa aacaccgcac gggtattcac cctgaaggaa atactcggtt tttcatccaa cgaaatacaa caaatgtgcg gtatcagcac gtccaactac cacaccatta tgcaccgcgc ccgagaatca ttgcgccaat gcctgcaaat caaatggttc aaccaagaaa acccgaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat