NegoA.17127.a

glucose-6-phosphate isomerase 1

CENTER ID: NegoA.17127.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17127.a.B1.GE40059 full length 1 548
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.17127.a.B1.PS37930 full length 1 548
Molecular models
METHOD RESULTS
comparative modelling Robetta_85045
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|521006.8.peg.1254
UniProt: B4RM09
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKHLHDLPAW SKLWNHFDDS KTLHMREMFE QDPQRAERYW LQVGGLTLDY SKNRINDETM SLLFELAREA GVPERMRQMF HGEKINTTEN RAVLHVALRN RTNSPIMVDG EDVMPKVNRV LQRMGEFAHE VRSGSWLGYT NQVITDVVNI GIGGSDLGPL TMCTALKPFG HPRLNMHFVS NVDGSQLRDV LSKVHPETTL FIIASKTFTT QETLTNALTA REWFLNHAGD EEAVAKHFAA VSTNRKAVAE FGIDIANMFE FWDWVGGRYS LWSAIGLPIM LYLGEENFIE MLNGAHLMDQ HFINTPLERN LPVILALIGI WYINYYGGGS HVIAPYDQHL HRLPKFIQQL DMESNGKQVT LDGKAVGHET SPIIWGETGI NGQHAFFQLL HQGTHITPID LIASLEKRSN LPGHHEILLA NVFAQAEAFM CGKTPDEVRA ELKAQGMDEA RIEELVPHKT FSGNRPTNLI LMDKVNPRNM GSLIAMYEHK TFVQGIIWGI NSFDQWGVEL GKQLAKTILG ELTGETEPQK HDSSTERLIN LYLQTNRK
NT Sequence
ATGAAACACC TTCACGACTT ACCGGCCTGG TCAAAATTGT GGAACCACTT TGACGACAGC AAAACATTGC ATATGCGCGA AATGTTCGAG CAAGACCCGC AGCGGGCGGA ACGCTACTGG CTGCAAGTCG GCGGACTGAC GCTGGACTAC TCCAAAAACC GCATCAACGA CGAAACCATG TCCCTACTGT TCGAGTTGGC GCGTGAAGCA GGCGTGCCGG AGCGGATGCG GCAGATGTTC CACGGCGAAA AAATCAATAC CACCGAAAAC CGCGCCGTCC TGCATGTCGC CCTTCGCAAC CGCACCAATT CGCCGATTAT GGTTGACGGT GAAGATGTGA TGCCCAAAGT CAACCGCGTT TTGCAACGTA TGGGCGAATT TGCACACGAA GTCCGCAGCG GAAGCTGGCT GGGCTATACC AACCAAGTCA TTACCGACGT TGTCAACATC GGCATCGGCG GATCGGATTT GGGCCCGCTG ACGATGTGTA CCGCGCTCAA ACCTTTCGGT CATCCGCGCC TCAATATGCA CTTCGTCTCC AACGTGGACG GCTCGCAACT GCGCGACGTA TTGTCCAAAG TCCACCCGGA AACCACGCTG TTCATCATCG CCTCCAAAAC ATTTACCACG CAGGAAACGC TGACCAACGC GCTAACCGCG CGCGAATGGT TTTTGAATCA TGCGGGTGAC GAAGAAGCCG TTGCCAAACA CTTCGCCGCC GTTTCCACCA ATCGAAAAGC CGTCGCCGAA TTCGGCATCG ACATCGCCAA TATGTTTGAA TTTTGGGATT GGGTCGGCGG TCGGTACAGC CTGTGGTCCG CCATCGGATT GCCGATTATG CTGTATCTCG GCGAAGAAAA CTTCATCGAA ATGCTCAACG GCGCGCACCT GATGGACCAA CACTTCATCA ACACCCCGCT CGAGCGCAAC CTGCCCGTCA TCCTCGCCCT CATCGGCATC TGGTATATCA ACTACTACGG TGGCGGCAGC CACGTCATCG CGCCTTACGA CCAACATTTG CACCGCCTGC CCAAATTCAT CCAGCAGCTC GATATGGAAA GCAACGGCAA ACAGGTTACG TTGGACGGCA AAGCAGTCGG ACACGAAACC TCGCCGATTA TCTGGGGAGA AACGGGCATT AACGGTCAGC ACGCCTTCTT CCAACTGCTG CATCAAGGCA CGCACATCAC CCCCATCGAC CTGATTGCCT CGCTTGAAAA ACGCAGCAAC CTGCCCGGAC ACCACGAAAT CCTGCTTGCC AACGTCTTTG CACAAGCAGA AGCCTTTATG TGCGGCAAAA CCCCCGACGA AGTCCGCGCC GAACTCAAAG CGCAGGGTAT GGATGAGGCG CGCATCGAAG AGCTGGTTCC GCACAAAACC TTCTCCGGCA ACCGCCCGAC CAACCTCATC CTGATGGATA AGGTCAACCC CCGCAATATG GGCAGCCTGA TTGCCATGTA CGAACACAAA ACCTTCGTCC AAGGCATCAT TTGGGGCATC AACAGCTTCG ACCAGTGGGG CGTGGAACTC GGCAAACAAC TGGCTAAAAC CATTTTGGGC GAACTGACAG GCGAAACCGA ACCTCAAAAA CACGACAGCT CGACCGAACG CCTGATTAAC CTCTACCTGC AGACCAACCG CAAATAA
Details for NegoA.17127.a.B1.GE40059
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 63kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MKHLHDLPAW SKLWNHFDDS KTLHMREMFE QDPQRAERYW LQVGGLTLDY SKNRINDETM SLLFELAREA GVPERMRQMF HGEKINTTEN RAVLHVALRN RTNSPIMVDG EDVMPKVNRV LQRMGEFAHE VRSGSWLGYT NQVITDVVNI GIGGSDLGPL TMCTALKPFG HPRLNMHFVS NVDGSQLRDV LSKVHPETTL FIIASKTFTT QETLTNALTA REWFLNHAGD EEAVAKHFAA VSTNRKAVAE FGIDIANMFE FWDWVGGRYS LWSAIGLPIM LYLGEENFIE MLNGAHLMDQ HFINTPLERN LPVILALIGI WYINYYGGGS HVIAPYDQHL HRLPKFIQQL DMESNGKQVT LDGKAVGHET SPIIWGETGI NGQHAFFQLL HQGTHITPID LIASLEKRSN LPGHHEILLA NVFAQAEAFM CGKTPDEVRA ELKAQGMDEA RIEELVPHKT FSGNRPTNLI LMDKVNPRNM GSLIAMYEHK TFVQGIIWGI NSFDQWGVEL GKQLAKTILG ELTGETEPQK HDSSTERLIN
Validated NT Sequence
ggttaatcag gcgttcggtc gagctgtcgt gtttttgagg ttcggtttcg cctgtcagtt cgcccaaaat ggttttagcc agttgtttgc cgagttccac gccccactgg tcgaagctgt tgatgcccca aatgatgcct tggacgaagg ttttgtgttc gtacatggca atcaggctgc ccatattgcg ggggttgacc ttatccatca ggatgaggtt ggtcgggcgg ttgccggaga aggttttgtg cggaaccagc tcttcgatgc gcgcctcatc cataccctgc gctttgagtt cggcgcggac ttcgtcgggg gttttgccgc acataaaggc ttctgcttgt gcaaagacgt tggcaagcag gatttcgtgg tgtccgggca ggttgctgcg tttttcaagc gaggcaatca ggtcgatggg ggtgatgtgc gtgccttgat gcagcagttg gaagaaggcg tgctgaccgt taatgcccgt ttctccccag ataatcggcg aggtttcgtg tccgactgct ttgccgtcca acgtaacctg tttgccgttg ctttccatat cgagctgctg gatgaatttg ggcaggcggt gcaaatgttg gtcgtaaggc gcgatgacgt ggctgccgcc accgtagtag ttgatatacc agatgccgat gagggcgagg atgacgggca ggttgcgctc gagcggggtg ttgatgaagt gttggtccat caggtgcgcg ccgttgagca tttcgatgaa gttttcttcg ccgagataca gcataatcgg caatccgatg gcggaccaca ggctgtaccg accgccgacc caatcccaaa attcaaacat attggcgatg tcgatgccga attcggcgac ggcttttcga ttggtggaaa cggcggcgaa gtgtttggca acggcttctt cgtcacccgc atgattcaaa aaccattcgc gcgcggttag cgcgttggtc agcgtttcct gcgtggtaaa tgttttggag gcgatgatga acagcgtggt ttccgggtgg actttggaca atacgtcgcg cagttgcgag ccgtccacgt tggagacgaa gtgcatattg aggcgcggat gaccgaaagg tttgagcgcg gtacacatcg tcagcgggcc caaatccgat ccgccgatgc cgatgttgac aacgtcggta atgacttggt tggtatagcc cagccagctt ccgctgcgga cttcgtgtgc aaattcgccc atacgttgca aaacgcggtt gactttgggc atcacatctt caccgtcaac cataatcggc gaattggtgc ggttgcgaag ggcgacatgc aggacggcgc ggttttcggt ggtattgatt ttttcgccgt ggaacatctg ccgcatccgc tccggcacgc ctgcttcacg cgccaactcg aacagtaggg acatggtttc gtcgttgatg cggtttttgg agtagtccag cgtcagtccg ccgacttgca gccagtagcg ttccgcccgc tgcgggtctt gctcgaacat ttcgcgcata tgcaatgttt tgctgtcgtc aaagtggttc cacaattttg accaggccgg taagtcgtga aggtgtttca tatggtggtg gtggtggtga
Expected Protein Sequence
MAHHHHHHMK HLHDLPAWSK LWNHFDDSKT LHMREMFEQD PQRAERYWLQ VGGLTLDYSK NRINDETMSL LFELAREAGV PERMRQMFHG EKINTTENRA VLHVALRNRT NSPIMVDGED VMPKVNRVLQ RMGEFAHEVR SGSWLGYTNQ VITDVVNIGI GGSDLGPLTM CTALKPFGHP RLNMHFVSNV DGSQLRDVLS KVHPETTLFI IASKTFTTQE TLTNALTARE WFLNHAGDEE AVAKHFAAVS TNRKAVAEFG IDIANMFEFW DWVGGRYSLW SAIGLPIMLY LGEENFIEML NGAHLMDQHF INTPLERNLP VILALIGIWY INYYGGGSHV IAPYDQHLHR LPKFIQQLDM ESNGKQVTLD GKAVGHETSP IIWGETGING QHAFFQLLHQ GTHITPIDLI ASLEKRSNLP GHHEILLANV FAQAEAFMCG KTPDEVRAEL KAQGMDEARI EELVPHKTFS GNRPTNLILM DKVNPRNMGS LIAMYEHKTF VQGIIWGINS FDQWGVELGK QLAKTILGEL TGETEPQKHD SSTERLINLY LQTNRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac accttcacga cttaccggcc tggtcaaaat tgtggaacca ctttgacgac agcaaaacat tgcatatgcg cgaaatgttc gagcaagacc cgcagcgggc ggaacgctac tggctgcaag tcggcggact gacgctggac tactccaaaa accgcatcaa cgacgaaacc atgtccctac tgttcgagtt ggcgcgtgaa gcaggcgtgc cggagcggat gcggcagatg ttccacggcg aaaaaatcaa taccaccgaa aaccgcgccg tcctgcatgt cgcccttcgc aaccgcacca attcgccgat tatggttgac ggtgaagatg tgatgcccaa agtcaaccgc gttttgcaac gtatgggcga atttgcacac gaagtccgca gcggaagctg gctgggctat accaaccaag tcattaccga cgttgtcaac atcggcatcg gcggatcgga tttgggcccg ctgacgatgt gtaccgcgct caaacctttc ggtcatccgc gcctcaatat gcacttcgtc tccaacgtgg acggctcgca actgcgcgac gtattgtcca aagtccaccc ggaaaccacg ctgttcatca tcgcctccaa aacatttacc acgcaggaaa cgctgaccaa cgcgctaacc gcgcgcgaat ggtttttgaa tcatgcgggt gacgaagaag ccgttgccaa acacttcgcc gccgtttcca ccaatcgaaa agccgtcgcc gaattcggca tcgacatcgc caatatgttt gaattttggg attgggtcgg cggtcggtac agcctgtggt ccgccatcgg attgccgatt atgctgtatc tcggcgaaga aaacttcatc gaaatgctca acggcgcgca cctgatggac caacacttca tcaacacccc gctcgagcgc aacctgcccg tcatcctcgc cctcatcggc atctggtata tcaactacta cggtggcggc agccacgtca tcgcgcctta cgaccaacat ttgcaccgcc tgcccaaatt catccagcag ctcgatatgg aaagcaacgg caaacaggtt acgttggacg gcaaagcagt cggacacgaa acctcgccga ttatctgggg agaaacgggc attaacggtc agcacgcctt cttccaactg ctgcatcaag gcacgcacat cacccccatc gacctgattg cctcgcttga aaaacgcagc aacctgcccg gacaccacga aatcctgctt gccaacgtct ttgcacaagc agaagccttt atgtgcggca aaacccccga cgaagtccgc gccgaactca aagcgcaggg tatggatgag gcgcgcatcg aagagctggt tccgcacaaa accttctccg gcaaccgccc gaccaacctc atcctgatgg ataaggtcaa cccccgcaat atgggcagcc tgattgccat gtacgaacac aaaaccttcg tccaaggcat catttggggc atcaacagct tcgaccagtg gggcgtggaa ctcggcaaac aactggctaa aaccattttg ggcgaactga caggcgaaac cgaacctcaa aaacacgaca gctcgaccga acgcctgatt aacctctacc tgcagaccaa ccgcaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for NegoA.17127.a.B1.PS37930
PURIFICATION DATe: 5/11/2016
CONCENTRATION: 30mg/ml
OBSERVED MW: 66kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MKHLHDLPAW SKLWNHFDDS KTLHMREMFE QDPQRAERYW LQVGGLTLDY SKNRINDETM SLLFELAREA GVPERMRQMF HGEKINTTEN RAVLHVALRN RTNSPIMVDG EDVMPKVNRV LQRMGEFAHE VRSGSWLGYT NQVITDVVNI GIGGSDLGPL TMCTALKPFG HPRLNMHFVS NVDGSQLRDV LSKVHPETTL FIIASKTFTT QETLTNALTA REWFLNHAGD EEAVAKHFAA VSTNRKAVAE FGIDIANMFE FWDWVGGRYS LWSAIGLPIM LYLGEENFIE MLNGAHLMDQ HFINTPLERN LPVILALIGI WYINYYGGGS HVIAPYDQHL HRLPKFIQQL DMESNGKQVT LDGKAVGHET SPIIWGETGI NGQHAFFQLL HQGTHITPID LIASLEKRSN LPGHHEILLA NVFAQAEAFM CGKTPDEVRA ELKAQGMDEA RIEELVPHKT FSGNRPTNLI LMDKVNPRNM GSLIAMYEHK TFVQGIIWGI NSFDQWGVEL GKQLAKTILG ELTGETEPQK HDSSTERLIN
Validated NT Sequence
ggttaatcag gcgttcggtc gagctgtcgt gtttttgagg ttcggtttcg cctgtcagtt cgcccaaaat ggttttagcc agttgtttgc cgagttccac gccccactgg tcgaagctgt tgatgcccca aatgatgcct tggacgaagg ttttgtgttc gtacatggca atcaggctgc ccatattgcg ggggttgacc ttatccatca ggatgaggtt ggtcgggcgg ttgccggaga aggttttgtg cggaaccagc tcttcgatgc gcgcctcatc cataccctgc gctttgagtt cggcgcggac ttcgtcgggg gttttgccgc acataaaggc ttctgcttgt gcaaagacgt tggcaagcag gatttcgtgg tgtccgggca ggttgctgcg tttttcaagc gaggcaatca ggtcgatggg ggtgatgtgc gtgccttgat gcagcagttg gaagaaggcg tgctgaccgt taatgcccgt ttctccccag ataatcggcg aggtttcgtg tccgactgct ttgccgtcca acgtaacctg tttgccgttg ctttccatat cgagctgctg gatgaatttg ggcaggcggt gcaaatgttg gtcgtaaggc gcgatgacgt ggctgccgcc accgtagtag ttgatatacc agatgccgat gagggcgagg atgacgggca ggttgcgctc gagcggggtg ttgatgaagt gttggtccat caggtgcgcg ccgttgagca tttcgatgaa gttttcttcg ccgagataca gcataatcgg caatccgatg gcggaccaca ggctgtaccg accgccgacc caatcccaaa attcaaacat attggcgatg tcgatgccga attcggcgac ggcttttcga ttggtggaaa cggcggcgaa gtgtttggca acggcttctt cgtcacccgc atgattcaaa aaccattcgc gcgcggttag cgcgttggtc agcgtttcct gcgtggtaaa tgttttggag gcgatgatga acagcgtggt ttccgggtgg actttggaca atacgtcgcg cagttgcgag ccgtccacgt tggagacgaa gtgcatattg aggcgcggat gaccgaaagg tttgagcgcg gtacacatcg tcagcgggcc caaatccgat ccgccgatgc cgatgttgac aacgtcggta atgacttggt tggtatagcc cagccagctt ccgctgcgga cttcgtgtgc aaattcgccc atacgttgca aaacgcggtt gactttgggc atcacatctt caccgtcaac cataatcggc gaattggtgc ggttgcgaag ggcgacatgc aggacggcgc ggttttcggt ggtattgatt ttttcgccgt ggaacatctg ccgcatccgc tccggcacgc ctgcttcacg cgccaactcg aacagtaggg acatggtttc gtcgttgatg cggtttttgg agtagtccag cgtcagtccg ccgacttgca gccagtagcg ttccgcccgc tgcgggtctt gctcgaacat ttcgcgcata tgcaatgttt tgctgtcgtc aaagtggttc cacaattttg accaggccgg taagtcgtga aggtgtttca tatggtggtg gtggtggtga
Expressed Protein Sequence
MAHHHHHHMK HLHDLPAWSK LWNHFDDSKT LHMREMFEQD PQRAERYWLQ VGGLTLDYSK NRINDETMSL LFELAREAGV PERMRQMFHG EKINTTENRA VLHVALRNRT NSPIMVDGED VMPKVNRVLQ RMGEFAHEVR SGSWLGYTNQ VITDVVNIGI GGSDLGPLTM CTALKPFGHP RLNMHFVSNV DGSQLRDVLS KVHPETTLFI IASKTFTTQE TLTNALTARE WFLNHAGDEE AVAKHFAAVS TNRKAVAEFG IDIANMFEFW DWVGGRYSLW SAIGLPIMLY LGEENFIEML NGAHLMDQHF INTPLERNLP VILALIGIWY INYYGGGSHV IAPYDQHLHR LPKFIQQLDM ESNGKQVTLD GKAVGHETSP IIWGETGING QHAFFQLLHQ GTHITPIDLI ASLEKRSNLP GHHEILLANV FAQAEAFMCG KTPDEVRAEL KAQGMDEARI EELVPHKTFS GNRPTNLILM DKVNPRNMGS LIAMYEHKTF VQGIIWGINS FDQWGVELGK QLAKTILGEL TGETEPQKHD SSTERLINLY LQTNRK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAC ACCTTCACGA CTTACCGGCC TGGTCAAAAT TGTGGAACCA CTTTGACGAC AGCAAAACAT TGCATATGCG CGAAATGTTC GAGCAAGACC CGCAGCGGGC GGAACGCTAC TGGCTGCAAG TCGGCGGACT GACGCTGGAC TACTCCAAAA ACCGCATCAA CGACGAAACC ATGTCCCTAC TGTTCGAGTT GGCGCGTGAA GCAGGCGTGC CGGAGCGGAT GCGGCAGATG TTCCACGGCG AAAAAATCAA TACCACCGAA AACCGCGCCG TCCTGCATGT CGCCCTTCGC AACCGCACCA ATTCGCCGAT TATGGTTGAC GGTGAAGATG TGATGCCCAA AGTCAACCGC GTTTTGCAAC GTATGGGCGA ATTTGCACAC GAAGTCCGCA GCGGAAGCTG GCTGGGCTAT ACCAACCAAG TCATTACCGA CGTTGTCAAC ATCGGCATCG GCGGATCGGA TTTGGGCCCG CTGACGATGT GTACCGCGCT CAAACCTTTC GGTCATCCGC GCCTCAATAT GCACTTCGTC TCCAACGTGG ACGGCTCGCA ACTGCGCGAC GTATTGTCCA AAGTCCACCC GGAAACCACG CTGTTCATCA TCGCCTCCAA AACATTTACC ACGCAGGAAA CGCTGACCAA CGCGCTAACC GCGCGCGAAT GGTTTTTGAA TCATGCGGGT GACGAAGAAG CCGTTGCCAA ACACTTCGCC GCCGTTTCCA CCAATCGAAA AGCCGTCGCC GAATTCGGCA TCGACATCGC CAATATGTTT GAATTTTGGG ATTGGGTCGG CGGTCGGTAC AGCCTGTGGT CCGCCATCGG ATTGCCGATT ATGCTGTATC TCGGCGAAGA AAACTTCATC GAAATGCTCA ACGGCGCGCA CCTGATGGAC CAACACTTCA TCAACACCCC GCTCGAGCGC AACCTGCCCG TCATCCTCGC CCTCATCGGC ATCTGGTATA TCAACTACTA CGGTGGCGGC AGCCACGTCA TCGCGCCTTA CGACCAACAT TTGCACCGCC TGCCCAAATT CATCCAGCAG CTCGATATGG AAAGCAACGG CAAACAGGTT ACGTTGGACG GCAAAGCAGT CGGACACGAA ACCTCGCCGA TTATCTGGGG AGAAACGGGC ATTAACGGTC AGCACGCCTT CTTCCAACTG CTGCATCAAG GCACGCACAT CACCCCCATC GACCTGATTG CCTCGCTTGA AAAACGCAGC AACCTGCCCG GACACCACGA AATCCTGCTT GCCAACGTCT TTGCACAAGC AGAAGCCTTT ATGTGCGGCA AAACCCCCGA CGAAGTCCGC GCCGAACTCA AAGCGCAGGG TATGGATGAG GCGCGCATCG AAGAGCTGGT TCCGCACAAA ACCTTCTCCG GCAACCGCCC GACCAACCTC ATCCTGATGG ATAAGGTCAA CCCCCGCAAT ATGGGCAGCC TGATTGCCAT GTACGAACAC AAAACCTTCG TCCAAGGCAT CATTTGGGGC ATCAACAGCT TCGACCAGTG GGGCGTGGAA CTCGGCAAAC AACTGGCTAA AACCATTTTG GGCGAACTGA CAGGCGAAAC CGAACCTCAA AAACACGACA GCTCGACCGA ACGCCTGATT AACCTCTACC TGCAGACCAA CCGCAAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT