NegoA.12707.a

squalene synthase HpnC

CENTER ID: NegoA.12707.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.12707.a.B1.GE40058 full length 1 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.878
UniProt: B4RL03

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVGHYENFP VGSLVLPRRL RKPVHAVYAF ARTADDMADE GSMPSEARLS GLEGLRRELD VLASGGRSAH PLIARLDAEA VVPFGLDLQP FYDLLSAFSQ DVVKTRYAHF GDLTDYCRRS ANPVGRIMLA LYGKTDAVCV AQSDGICTAL QLVNFWQDVA VDWQKGRVYI PQDDLLKFGV SEEQIAAGRA DAAFQRLMAY ECRRAFRMLK AGSPLARELK GRIGLELRMI VLGAQLILQK LDACRYDVFA QRPVLDKKDW LIMLKRALWK
NT Sequence
ATGTCGGTTG GGCATTATGA GAATTTTCCC GTCGGTTCGC TGGTTTTGCC GCGCAGGTTG AGGAAGCCGG TTCATGCGGT GTATGCGTTT GCACGGACGG CGGACGATAT GGCGGACGAG GGCAGTATGC CGTCTGAAGC CAGGTTGTCG GGGTTGGAGG GTTTGCGGCG CGAGTTGGAC GTGTTGGCAT CGGGCGGCCG GTCGGCGCAT CCTTTGATTG CACGATTGGA TGCCGAGGCG GTTGTGCCGT TCGGTTTGGA TTTGCAGCCG TTTTATGATC TGCTCTCGGC GTTTTCGCAG GATGTGGTTA AAACGCGGTA CGCGCATTTC GGCGATCTGA CCGATTATTG CCGGCGTTCC GCCAACCCTG TCGGACGCAT TATGCTGGCT TTATACGGGA AAACGGATGC GGTGTGCGTA GCGCAAAGCG ACGGTATTTG TACGGCTTTG CAACTGGTGA ATTTTTGGCA GGATGTAGCT GTGGATTGGC AAAAGGGCAG GGTTTATATC CCGCAGGACG ATTTGTTGAA ATTCGGTGTT TCTGAGGAAC AGATCGCAGC AGGACGGGCG GATGCGGCGT TTCAGCGGCT GATGGCGTAT GAGTGCCGGC GTGCATTCCG TATGCTGAAG GCGGGTTCGC CTTTGGCGCG CGAATTGAAA GGGCGTATCG GTTTGGAACT CCGTATGATT GTGTTGGGGG CGCAGTTGAT TTTGCAGAAA CTGGACGCGT GCCGATACGA TGTGTTTGCA CAACGCCCCG TTTTGGATAA GAAGGATTGG TTGATTATGT TGAAACGCGC GTTGTGGAAA TGA
Details for NegoA.12707.a.B1.GE40058
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS VGHYENFPVG SLILPRRLRK PVHAVYAFAR TADDMADEGS MPSEARLSGL EGLRRELDVL ASGGRSAHPL IARLDAEAVV PFGLDLQPFY DLLSAFSQDV VKTRYAHFGD LTDYCRRSAN PVGRIMLALY GKTDAVCVAQ SDGICTALQL VNFWQDVAVD WQKGRVYIPQ DDLLKFGVSE EQIAAGRADA AFQRLMAYEC RRAFRMLKAG SPLARELKGR IGLELRMIVL GAQLILQKLD ACRYDVFAQR PVLDKKDWLI MLKRALWK
Validated NT Sequence
atcttactca cttatttcca caacgcgcgt ttcaacataa tcaaccaatc cttcttatcc aaaacggggc gttgtgcaaa cacatcgtat cggcacgcgt ccagtttctg caaaatcaac tgcgccccca acacaatcat acggagttcc aaaccgatac gccctttcaa ttcgcgcgcc aaaggcgaac ccgccttcag catacggaat gcacgccggc actcatacgc catcagccgc tgaaacgccg catccgcccg tcctgctgcg atctgttcct cagaaacacc gaatttcaac aaatcgtcct gcgggatata aaccctgccc ttttgccaat ccacagctac atcctgccaa aaattcacca gttgcaaagc cgtacaaata ccgtcgcttt gcgctacgca caccgcatcc gttttcccgt ataaagccag cataatgcgt ccgacagggt tggcggaacg ccggcaataa tcggtcagat cgccgaaatg cgcgtaccgc gttttaacca catcctgcga aaacgccgag agcagatcat aaaacggctg caaatccaaa ccgaacggca caaccgcctc ggcatccaat cgtgcaatca aaggatgcgc cgaccggccg cccgatgcca acacgtccaa ctcgcgccgc aaaccctcca accccgacaa cctggcttca gacggcatac tgccctcgtc cgccatatcg tccgccgtcc gtgcaaacgc atacaccgca tgaaccggct tcctcaacct gcgcggcaaa atcagcgaac cgacgggaaa attctcataa tgcccaaccg acatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS VGHYENFPVG SLVLPRRLRK PVHAVYAFAR TADDMADEGS MPSEARLSGL EGLRRELDVL ASGGRSAHPL IARLDAEAVV PFGLDLQPFY DLLSAFSQDV VKTRYAHFGD LTDYCRRSAN PVGRIMLALY GKTDAVCVAQ SDGICTALQL VNFWQDVAVD WQKGRVYIPQ DDLLKFGVSE EQIAAGRADA AFQRLMAYEC RRAFRMLKAG SPLARELKGR IGLELRMIVL GAQLILQKLD ACRYDVFAQR PVLDKKDWLI MLKRALWK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgg ttgggcatta tgagaatttt cccgtcggtt cgctggtttt gccgcgcagg ttgaggaagc cggttcatgc ggtgtatgcg tttgcacgga cggcggacga tatggcggac gagggcagta tgccgtctga agccaggttg tcggggttgg agggtttgcg gcgcgagttg gacgtgttgg catcgggcgg ccggtcggcg catcctttga ttgcacgatt ggatgccgag gcggttgtgc cgttcggttt ggatttgcag ccgttttatg atctgctctc ggcgttttcg caggatgtgg ttaaaacgcg gtacgcgcat ttcggcgatc tgaccgatta ttgccggcgt tccgccaacc ctgtcggacg cattatgctg gctttatacg ggaaaacgga tgcggtgtgc gtagcgcaaa gcgacggtat ttgtacggct ttgcaactgg tgaatttttg gcaggatgta gctgtggatt ggcaaaaggg cagggtttat atcccgcagg acgatttgtt gaaattcggt gtttctgagg aacagatcgc agcaggacgg gcggatgcgg cgtttcagcg gctgatggcg tatgagtgcc ggcgtgcatt ccgtatgctg aaggcgggtt cgcctttggc gcgcgaattg aaagggcgta tcggtttgga actccgtatg attgtgttgg gggcgcagtt gattttgcag aaactggacg cgtgccgata cgatgtgttt gcacaacgcc ccgttttgga taagaaggat tggttgatta tgttgaaacg cgcgttgtgg aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat