SSGCID: Target Page

NegoA.10537.a

cell division protein ftsA

CENTER ID:	NegoA.10537.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.10537.a.B1.GE40057	full length		1	414

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.1940
UniProt:	B4RQC1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEQQKRYISV LDIGTSKVLA LIGEVQDDDK INIIGLGQAP SRGLRAGMVT NIDATVQAIR QAVNDAELMA DTKITHVTTG IAGNHIRSLN SQGVVKIKDG EVTQADIDRA IETAKAINIP PDQKILDAVV QDYIIDTQLG VREPIGMSGV RLDTRVHIIT GASTAVQNVQ KCIELCGLKS DQIMLQPLAS GQAVLTEDEK DLGVCVIDIG GGTTDIAVYM NGAIRHTSVI PAGGNLITKD LSKSLRTPLD AAEYIKIHYG VASCDTEGLG EMIEVPGVGD RTSRQVSSKV LAAIISARIQ EIFGVVLGEL QKSGFPKEVL NAGIVLTGGV SMMTGIVEFA EKIFDLPVRT GAPQEMGGLS DRVRTPRFST AIGLLHAACK LEGNLPQPEN GTVQEREGGG GLLARLKRWI ENNL

NT Sequence

ATGGAACAGC AGAAAAGATA CATCAGCGTA CTGGATATCG GTACGTCTAA AGTCCTCGCA CTGATCGGGG AAGTTCAAGA TGACGACAAA ATCAACATCA TCGGTTTGGG GCAGGCTCCT TCACGGGGCT TGCGCGCGGG CATGGTAACC AATATCGATG CCACCGTCCA AGCCATCAGG CAGGCGGTCA ATGATGCCGA GCTGATGGCG GATACCAAAA TTACCCACGT TACCACAGGT ATCGCAGGCA ACCACATCCG CAGCCTTAAT TCGCAAGGTG TGGTTAAAAT CAAAGACGGA GAAGTAACAC AGGCAGACAT CGACCGCGCC ATTGAAACGG CAAAGGCAAT CAATATCCCG CCCGATCAAA AAATTCTCGA TGCCGTGGTT CAAGACTACA TTATCGACAC CCAACTTGGT GTAAGGGAGC CCATCGGTAT GAGCGGTGTG CGTCTGGATA CGCGGGTGCA CATCATTACC GGTGCAAGTA CGGCAGTGCA GAATGTCCAA AAATGTATCG AGCTGTGCGG CTTGAAAAGC GATCAGATCA TGCTTCAACC GTTGGCAAGC GGGCAGGCGG TGCTGACTGA AGATGAAAAA GACCTCGGCG TATGCGTCAT CGACATTGGT GGCGGAACGA CCGATATTGC CGTTTATATG AACGGCGCCA TCCGCCATAC GTCCGTCATT CCGGCCGGTG GTAATCTGAT TACCAAAGAT TTGTCCAAAT CGTTGAGAAC ACCTCTCGAT GCCGCCGAGT ACATTAAAAT CCATTATGGC GTGGCATCAT GCGATACGGA AGGCTTGGGC GAGATGATTG AAGTTCCGGG CGTGGGTGAC CGGACATCGC GTCAGGTTTC CAGTAAGGTT TTGGCAGCAA TCATCAGCGC ACGTATTCAG GAGATTTTTG GCGTAGTGCT GGGCGAACTG CAAAAATCGG GTTTCCCCAA AGAAGTGCTG AATGCGGGTA TCGTTCTGAC CGGCGGTGTG TCCATGATGA CCGGGATTGT GGAATTTGCC GAAAAAATCT TCGATTTGCC TGTACGCACC GGTGCGCCCC AAGAAATGGG CGGTTTGTCC GACCGTGTCC GCACACCGCG CTTTTCTACC GCTATCGGGC TGCTTCATGC AGCATGCAAG CTGGAAGGAA ACTTGCCGCA ACCGGAAAAC GGTACAGTGC AAGAGAGAGA AGGGGGCGGC GGTTTGTTGG CAAGATTGAA GCGGTGGATT GAAAACAACC TCTGA

Details for NegoA.10537.a.B1.GE40057

HARVESTED ON:	data unavailable
SEQUENCED ON:	3/25/2016
EXPECTED MW:	45kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHME QQKRYISVLD IGTSKVLALI GEVQDDDKIN IIGLGQAPSR GLRAGMVTNI DATVQAIRQA VNDAELMADT KITHVTTGIA GNHIRSLNSQ GVVKIKDGEV TQADIDRAIE TAKAINIPPD QKILDAVVQD YIIDTQLGVR EPIGMSGVRL DTRVHIITGA STAVQNVQKC IELCGLKSDQ IMLQPLASGQ AVLTEDEKDL GVCVIDIGGG TTDIAVYMNG AIRHTSVIPA GGNLITKDLS KSLRTPLDAA EYIKIHYGVA SCDTEGLGEM IEVPGVGDRT SRQVSSKVLA AIISARIQEI FGVVLGELQK SGFPKEVLNA GIVLTGGVSM MTGIVEFAEK IFDLPVRTGA PQEMGGLSDR VRTPRFSTAI GLLHAACKLE GNLPQPENGT VQEREGGGGL LARL

Validated NT Sequence

cttcaatctt gccaacaaac cgccgccccc ttctctctct tgcactgtac cgttttccgg ttgcggcaag tttccttcca gcttgcatgc tgcatgaagc agcccgatag cggtagaaaa gcgcggtgtg cggacacggt cggacaaacc gcccatttct tggggcgcac cggtgcgtac aggcaaatcg aagatttttt cggcaaattc cacaatcccg gtcatcatgg acacaccgcc ggtcagaacg atacccgcat tcagcacttc tttggggaaa cccgattttt gcagttcgcc cagcactacg ccaaaaatct cctgaatacg tgcgctgatg attgctgcca aaaccttact ggaaacctga cgcgatgtcc ggtcacccac gcccggaact tcaatcatct cgcccaagcc ttccgtatcg catgatgcca cgccataatg gattttaatg tactcggcgg catcgagagg tgttctcaac gatttggaca aatctttggt aatcagatta ccaccggccg gaatgacgga cgtatggcgg atggcgccgt tcatataaac ggcaatatcg gtcgttccgc caccaatgtc gatgacgcat acgccgaggt ctttttcatc ttcagtcagc accgcctgcc cgcttgccaa cggttgaagc atgatctgat cgcttttcaa gccgcacagc tcgatacatt tttggacatt ctgcactgcc gtacttgcac cggtaatgat gtgcacccgc gtatccagac gcacaccgct cataccgatg ggctccctta caccaagttg ggtgtcgata atgtagtctt gaaccacggc atcgagaatt ttttgatcgg gcgggatatt gattgccttt gccgtttcaa tggcgcggtc gatgtctgcc tgtgttactt ctccgtcttt gattttaacc acaccttgcg aattaaggct gcggatgtgg ttgcctgcga tacctgtggt aacgtgggta attttggtat ccgccatcag ctcggcatca ttgaccgcct gcctgatggc ttggacggtg gcatcgatat tggttaccat gcccgcgcgc aagccccgtg aaggagcctg ccccaaaccg atgatgttga ttttgtcgtc atcttgaact tccccgatca gtgcgaggac tttagacgta ccgatatcca gtacgctgat gtatcttttc tgctgttcca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaac agcagaaaag atacatcagc gtactggata tcggtacgtc taaagtcctc gcactgatcg gggaagttca agatgacgac aaaatcaaca tcatcggttt ggggcaggct ccttcacggg gcttgcgcgc gggcatggta accaatatcg atgccaccgt ccaagccatc aggcaggcgg tcaatgatgc cgagctgatg gcggatacca aaattaccca cgttaccaca ggtatcgcag gcaaccacat ccgcagcctt aattcgcaag gtgtggttaa aatcaaagac ggagaagtaa cacaggcaga catcgaccgc gccattgaaa cggcaaaggc aatcaatatc ccgcccgatc aaaaaattct cgatgccgtg gttcaagact acattatcga cacccaactt ggtgtaaggg agcccatcgg tatgagcggt gtgcgtctgg atacgcgggt gcacatcatt accggtgcaa gtacggcagt gcagaatgtc caaaaatgta tcgagctgtg cggcttgaaa agcgatcaga tcatgcttca accgttggca agcgggcagg cggtgctgac tgaagatgaa aaagacctcg gcgtatgcgt catcgacatt ggtggcggaa cgaccgatat tgccgtttat atgaacggcg ccatccgcca tacgtccgtc attccggccg gtggtaatct gattaccaaa gatttgtcca aatcgttgag aacacctctc gatgccgccg agtacattaa aatccattat ggcgtggcat catgcgatac ggaaggcttg ggcgagatga ttgaagttcc gggcgtgggt gaccggacat cgcgtcaggt ttccagtaag gttttggcag caatcatcag cgcacgtatt caggagattt ttggcgtagt gctgggcgaa ctgcaaaaat cgggtttccc caaagaagtg ctgaatgcgg gtatcgttct gaccggcggt gtgtccatga tgaccgggat tgtggaattt gccgaaaaaa tcttcgattt gcctgtacgc accggtgcgc cccaagaaat gggcggtttg tccgaccgtg tccgcacacc gcgcttttct accgctatcg ggctgcttca tgcagcatgc aagctggaag gaaacttgcc gcaaccggaa aacggtacag tgcaagagag agaagggggc ggcggtttgt tggcaagatt gaagcggtgg attgaaaaca acctctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat