NegoA.10154.a

Histone deacetylase family protein

CENTER ID: NegoA.10154.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.10154.a.B2.GE40238 mature protein 23 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.351
UniProt: B4RJK6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPLTRLFLKL YALLRLFLGK NAQTAWIFHP SCTGHDPGAN HPDSPDRILC IEQALRRAGI WQHLQTVEAE EISDTRLALV RSSKYLNRLE SCLPEDGKIC RLDDDTVISK NSLSAARFSA GSAVQAVDMV MKRKAWHAFC AARPPEHHAK SGKAGGFCLL NNVAAGVMHA IAEYRLKRIA VIDFDVHYGD GTAEIFKDDP RILFFNLFET DLFPFPENNG MPDGGNMVHL PLPSGTGSRT FREAVRRQWL PRLAAFKPEL VLLSAGFDAH RLDESGRLNL HEADFAWLTH KIIQTASSCP GKIVSVLEGG YTLEPLAQSA AEHIRVLAGL GKSDAATAYQ KTLNPTKKRF AKPKTGQVRQ PTQSDRYDIN AV
NT Sequence
ATGCCGCTGA CCCGCCTGTT CCTGAAACTC TACGCCCTGT TGCGCCTTTT TTTGGGCAAA AACGCCCAGA CCGCCTGGAT TTTCCACCCC TCCTGCACCG GGCACGACCC CGGGGCAAAC CATCCCGATT CGCCCGACCG CATCCTCTGC ATCGAGCAGG CATTGCGCCG CGCCGGTATT TGGCAGCACC TCCAAACCGT AGAGGCGGAA GAAATCAGCG ATACGCGCCT TGCACTTGTC CGCTCGAGCA AATATCTGAA CCGTTTGGAA TCTTGCCTAC CCGAAGACGG CAAGATTTGC CGTTTGGACG ACGACACCGT TATCAGCAAA AACTCCCTGT CCGCCGCCCG TTTTTCCGCC GGTTCGGCAG TTCAGGCAGT CGATATGGTC ATGAAACGCA AAGCGTGGCA CGCCTTTTGC GCCGCCCGCC CGCCGGAGCA TCACGCGAAA AGCGGCAAAG CCGGCGGATT CTGCCTGCTG AACAACGTTG CCGCCGGCGT CATGCATGCC ATTGCCGAAT ACCGCCTGAA ACGCATTGCC GTCATCGATT TCGATGTCCA CTACGGCGAC GGTACGGCAG AAATATTCAA AGACGATCCG CGTATCCTGT TTTTCAACCT GTTTGAAACC GACCTTTTCC CCTTCCCCGA AAACAACGGT ATGCCCGACG GCGGCAATAT GGTGCACCTG CCCTTGCCGT CGGGAACGGG CAGCCGCACA TTCCGCGAAG CCGTCCGCAG ACAGTGGCTG CCCCGGCTTG CCGCATTCAA ACCCGAACTG GTGCTGCTGT CGGCAGGATT CGACGCACAC CGTCTGGACG AATCGGGCAG GCTCAACCTG CACGAGGCGG ATTTTGCCTG GCTGACACAC AAAATCATTC AGACGGCATC CAGCTGCCCC GGCAAAATCG TATCCGTACT CGAAGGCGGC TACACCCTTG AACCTCTGGC ACAATCTGCT GCCGAGCATA TCCGCGTACT GGCGGGGCTG GGCAAATCCG ATGCCGCAAC CGCCTATCAG AAAACACTGA ACCCAACAAA AAAACGGTTT GCCAAACCGA AAACCGGACA GGTGCGCCAA CCTACCCAAT CCGACCGTTA CGATATAAAT GCCGTCTGA
Details for NegoA.10154.a.B2.GE40238
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMX XAWIFHPACT GHDPGANHPD SPDRILCIEQ ALRRAGIWQH LQTVEAEEIS DTRLALVRSS KYLNRLESCL PEDGKICRLD DDTVISKNSL SAARFSAGSA VQAVDMVMKR KAWHAFCAAR PPEHHAKSGK AGGFCLLNNV AAGVMHAIAE YRLKRIAVID FDVHYGDGTA EIFKDDPRIL FFNLFETDLF PFPENNGMPD GGNMVHLPLP SGTGSRTFRE AVRRQWLPRL AAFKPELVLL SAGFDAHRLD ESGRLNLHEA DFAWLTHKII QTASSCPGKI VSVLEGGYTL EPLAQSAAEH IRVLAGLGKS DAATAYQKTL NPTKKRFAKP KTGQVRQPTQ
Validated NT Sequence
attgggtagg ttggcgcacc tgtccggttt tcggtttggc aaaccgtttt tttgttgggt tcagtgtttt ctgataggcg gttgcggcat cggatttgcc cagccccgcc agtacgcgga tatgctcggc agcagattgt gccagaggtt caagggtgta gccgccttcg agtacggata cgattttgcc ggggcagctg gatgccgtct gaatgatttt gtgtgtcagc caggcaaaat ccgcctcgtg caggttgagc ctgcccgatt cgtccagacg gtgtgcgtcg aatcctgccg acagcagcac cagttcgggt ttgaatgcgg caagccgggg cagccactgt ctgcggacgg cttcgcggaa tgtgcggctg cccgttcccg acggcaaggg caggtgcacc atattgccgc cgtcgggcat accgttgttt tcggggaagg ggaaaaggtc ggtttcaaac aggttgaaaa acaggatgcg cggatcgtct ttgaatattt ctgccgtacc gtcgccgtag tggacatcga aatcgatgac ggcaatgcgt ttcaggcggt attcggcaat ggcatgcatg acgccggcgg caacgttgtt cagcaggcag aatccgccgg ctttgccgct tttcgcgtga tgctccggcg ggcgggcggc gcaaaaggcg tgccacgctt tgcgtttcat gaccatatcg actgcctgaa ctgccgaacc ggcggaaaaa cgggcggcgg acagggagtt tttgctgata acggtgtcgt cgtccaaacg gcaaatcttg ccgtcttcgg gtaggcaaga ttccaaacgg ttcagatatt tgctcgagcg gacaagtgca aggcgcgtat cgctgatttc ttccgcctct acggtttgga ggtgctgcca aataccggcg cggcgcaatg cctgctcgat gcagaggatg cggtcgggcg aatcgggatg gtttgccccg gggtcgtgcc cggtgcaggc ggggtggaaa atccaggcgg nnngcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHQT AWIFHPSCTG HDPGANHPDS PDRILCIEQA LRRAGIWQHL QTVEAEEISD TRLALVRSSK YLNRLESCLP EDGKICRLDD DTVISKNSLS AARFSAGSAV QAVDMVMKRK AWHAFCAARP PEHHAKSGKA GGFCLLNNVA AGVMHAIAEY RLKRIAVIDF DVHYGDGTAE IFKDDPRILF FNLFETDLFP FPENNGMPDG GNMVHLPLPS GTGSRTFREA VRRQWLPRLA AFKPELVLLS AGFDAHRLDE SGRLNLHEAD FAWLTHKIIQ TASSCPGKIV SVLEGGYTLE PLAQSAAEHI RVLAGLGKSD AATAYQKTLN PTKKRFAKPK TGQVRQPTQS DRYDINAV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagaccg cctggatttt ccacccctcc tgcaccgggc acgaccccgg ggcaaaccat cccgattcgc ccgaccgcat cctctgcatc gagcaggcat tgcgccgcgc cggtatttgg cagcacctcc aaaccgtaga ggcggaagaa atcagcgata cgcgccttgc acttgtccgc tcgagcaaat atctgaaccg tttggaatct tgcctacccg aagacggcaa gatttgccgt ttggacgacg acaccgttat cagcaaaaac tccctgtccg ccgcccgttt ttccgccggt tcggcagttc aggcagtcga tatggtcatg aaacgcaaag cgtggcacgc cttttgcgcc gcccgcccgc cggagcatca cgcgaaaagc ggcaaagccg gcggattctg cctgctgaac aacgttgccg ccggcgtcat gcatgccatt gccgaatacc gcctgaaacg cattgccgtc atcgatttcg atgtccacta cggcgacggt acggcagaaa tattcaaaga cgatccgcgt atcctgtttt tcaacctgtt tgaaaccgac cttttcccct tccccgaaaa caacggtatg cccgacggcg gcaatatggt gcacctgccc ttgccgtcgg gaacgggcag ccgcacattc cgcgaagccg tccgcagaca gtggctgccc cggcttgccg cattcaaacc cgaactggtg ctgctgtcgg caggattcga cgcacaccgt ctggacgaat cgggcaggct caacctgcac gaggcggatt ttgcctggct gacacacaaa atcattcaga cggcatccag ctgccccggc aaaatcgtat ccgtactcga aggcggctac acccttgaac ctctggcaca atctgctgcc gagcatatcc gcgtactggc ggggctgggc aaatccgatg ccgcaaccgc ctatcagaaa acactgaacc caacaaaaaa acggtttgcc aaaccgaaaa ccggacaggt gcgccaacct acccaatccg accgttacga tataaatgcc gtctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat