NegoA.10100.a

peptidase Do

CENTER ID: NegoA.10100.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.10100.a.B2.GE40237 mature protein 25 499
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.206
UniProt: B4RQ70

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFKKYQYFAL AALCAALLAG CEKAGSFFGA DKKEASFVER IEHTKDDGSV SMLLPDFAQL VQSEGPAVVN IQAAPAPRTQ NGSGNAETDS DPLADSDPFY EFFKRLVPNM PEIPQEEADD GGLNFGSGFI ISKNGYILTN THVVAGMGSI KVLLNDKREY TAKLIGSDVQ SDVALLKIDA TEELPVVKIG NPKNLKPGEW VAAIGAPFGF DNSVTAGIVS AKGRSLPNES YTPFIQTDVA INPGNSGGPL FNLKGQVVGI NSQIYSRSGG FMGISFAIPI DVAMNVAEQL KNTGKVQRGQ LGVIIQEVSY GLAQSFGLDK ASGALIAKIL PGSPAERAGL QAGDIVLSLD GGEIRSSGDL PVMVGAITPG KEVSLGVWRK GEEITIKAKL GNAAEHTGAS SKTDEAPYTE QQSGTFSVES AGITLQTHTD SSGKHLVVVR VSDAAERAGL RRGDEILAVG QVPVNDEAGF RKAMDKAGKN VPLLVMRRGN TLFIALNLQ
NT Sequence
GTGTTCAAAA AATACCAATA CTTCGCTTTG GCGGCACTGT GTGCCGCCTT GCTGGCAGGC TGCGAAAAGG CAGGCAGCTT TTTCGGTGCG GACAAAAAAG AAGCATCCTT CGTAGAACGC ATCGAACACA CCAAAGACGA CGGCAGTGTC AGTATGCTGC TGCCCGACTT TGCCCAACTG GTTCAAAGCG AAGGCCCGGC AGTCGTCAAT ATTCAGGCAG CCCCCGCCCC GCGCACCCAA AACGGCAGCG GCAATGCCGA AACCGATTCC GACCCGCTTG CCGACAGCGA CCCGTTCTAC GAATTTTTCA AACGCCTCGT CCCGAACATG CCCGAAATCC CCCAAGAAGA AGCAGATGAC GGCGGATTGA ACTTCGGTTC GGGCTTCATC ATCAGCAAAA ACGGCTACAT CCTGACCAAT ACCCACGTCG TTGCCGGTAT GGGCAGTATC AAAGTCCTGC TCAACGACAA GCGCGAATAT ACCGCCAAAC TCATCGGTTC GGATGTCCAA TCCGATGTCG CCCTTCTGAA AATCGACGCA ACGGAAGAGC TACCCGTCGT CAAAATCGGC AATCCCAAAA ATTTGAAACC GGGCGAATGG GTCGCTGCCA TCGGCGCGCC CTTCGGCTTT GACAACAGCG TGACCGCCGG CATCGTGTCC GCCAAAGGCA GAAGCCTGCC CAACGAAAGC TACACACCCT TCATCCAAAC CGACGTTGCC ATCAATCCGG GCAATTCCGG CGGCCCGCTG TTCAACTTAA AAGGACAGGT CGTCGGCATC AATTCGCAAA TATACAGCCG CAGCGGCGGA TTCATGGGCA TCTCCTTTGC CATCCCGATT GACGTTGCCA TGAATGTCGC CGAACAGCTG AAAAACACCG GCAAAGTCCA ACGCGGACAA CTGGGCGTGA TTATTCAGGA AGTATCCTAC GGTTTGGCAC AGTCGTTCGG TCTGGATAAA GCCAGCGGCG CATTGATTGC CAAAATCCTT CCCGGCAGCC CCGCAGAACG TGCCGGCCTG CAGGCGGGCG ACATCGTCCT CAGCCTCGAC GGCGGAGAAA TACGTTCTTC CGGCGACCTT CCCGTCATGG TCGGCGCCAT TACGCCGGGA AAAGAAGTCA GCCTCGGCGT ATGGCGCAAA GGCGAAGAAA TCACAATCAA AGCCAAGCTG GGCAACGCCG CCGAGCATAC CGGCGCATCA TCCAAAACAG ATGAAGCCCC CTACACCGAA CAGCAATCCG GTACGTTCTC GGTCGAATCC GCAGGCATTA CCCTTCAGAC ACATACCGAC AGCAGCGGCA AACACCTCGT CGTCGTACGG GTTTCCGACG CGGCAGAACG CGCAGGCTTA AGGCGCGGCG ACGAAATCCT CGCCGTCGGG CAAGTCCCCG TCAATGACGA AGCCGGTTTC CGCAAAGCTA TGGACAAGGC AGGCAAAAAC GTCCCCCTGC TGGTCATGCG CCGTGGCAAC ACGCTGTTCA TCGCATTAAA CCTGCAATAA
Details for NegoA.10100.a.B2.GE40237
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG SFFGADKKEA SFVERIEHTK DDGSVSMLLP DFAQLVQSEG PAVVNIQAAP APRTQNGSGN AETDSDPLAD SDPFYEFFKR LVPNMPEIPQ EEADDGGLNF GSGFIISKNG YILTNTHVVA GMGSIKVLLN DKREYTAKLI GSDVQSDVAL LKIDATEELP VVKIGNPKNL KPGEWVAAIG APFGFDNSVT AGIVSAKGRS LPNESYTPFI QTDVAINPGN SGGPLFNLKG QVVGINSQIY SRSGGFMGIS FAIPIDVAMN VAEQLKNTGK VQRGQLGVII QEVSYGLAQS FGLDKASGAL IAKILPGSPA ERAGLQAGDI VLSLDGGEIR SSGDLPVMVG AITPGKEVSL GVWRKGEEIT IKAKLGNAAE HTGASSKTDE APYTEQQSGT FSVESAGITL QTHTDSSGKH LVVVRVSDAA ERAGLRRGDE ILAVGQVPVN DEAGFRKAMD KAGKNVPLLV MRRGN
Validated NT Sequence
tgttgccacg gcgcatgacc agcaggggga cgtttttgcc tgccttgtcc atagctttgc ggaaaccggc ttcgtcattg acggggactt gcccgacggc gaggatttcg tcgccgcgcc ttaagcctgc gcgttctgcc gcgtcggaaa cccgtacgac gacgaggtgt ttgccgctgc tgtcggtatg tgtctgaagg gtaatgcctg cggattcgac cgagaacgta ccggattgct gttcggtgta gggggcttca tctgttttgg atgatgcgcc ggtatgctcg gcggcgttgc ccagcttggc tttgattgtg atttcttcgc ctttgcgcca tacgccgagg ctgacttctt ttcccggcgt aatggcgccg accatgacgg gaaggtcgcc ggaagaacgt atttctccgc cgtcgaggct gaggacgatg tcgcccgcct gcaggccggc acgttctgcg gggctgccgg gaaggatttt ggcaatcaat gcgccgctgg ctttatccag accgaacgac tgtgccaaac cgtaggatac ttcctgaata atcacgccca gttgtccgcg ttggactttg ccggtgtttt tcagctgttc ggcgacattc atggcaacgt caatcgggat ggcaaaggag atgcccatga atccgccgct gcggctgtat atttgcgaat tgatgccgac gacctgtcct tttaagttga acagcgggcc gccggaattg cccggattga tggcaacgtc ggtttggatg aagggtgtgt agctttcgtt gggcaggctt ctgcctttgg cggacacgat gccggcggtc acgctgttgt caaagccgaa gggcgcgccg atggcagcga cccattcgcc cggtttcaaa tttttgggat tgccgatttt gacgacgggt agctcttccg ttgcgtcgat tttcagaagg gcgacatcgg attggacatc cgaaccgatg agtttggcgg tatattcgcg cttgtcgttg agcaggactt tgatactgcc cataccggca acgacgtggg tattggtcag gatgtagccg tttttgctga tgatgaagcc cgaaccgaag ttcaatccgc cgtcatctgc ttcttcttgg gggatttcgg gcatgttcgg gacgaggcgt ttgaaaaatt cgtagaacgg gtcgctgtcg gcaagcgggt cggaatcggt ttcggcattg ccgctgccgt tttgggtgcg cggggcgggg gctgcctgaa tattgacgac tgccgggcct tcgctttgaa ccagttgggc aaagtcgggc agcagcatac tgacactgcc gtcgtctttg gtgtgttcga tgcgttctac gaaggatgct tcttttttgt ccgcaccgaa aaagctgccc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHGS FFGADKKEAS FVERIEHTKD DGSVSMLLPD FAQLVQSEGP AVVNIQAAPA PRTQNGSGNA ETDSDPLADS DPFYEFFKRL VPNMPEIPQE EADDGGLNFG SGFIISKNGY ILTNTHVVAG MGSIKVLLND KREYTAKLIG SDVQSDVALL KIDATEELPV VKIGNPKNLK PGEWVAAIGA PFGFDNSVTA GIVSAKGRSL PNESYTPFIQ TDVAINPGNS GGPLFNLKGQ VVGINSQIYS RSGGFMGISF AIPIDVAMNV AEQLKNTGKV QRGQLGVIIQ EVSYGLAQSF GLDKASGALI AKILPGSPAE RAGLQAGDIV LSLDGGEIRS SGDLPVMVGA ITPGKEVSLG VWRKGEEITI KAKLGNAAEH TGASSKTDEA PYTEQQSGTF SVESAGITLQ THTDSSGKHL VVVRVSDAAE RAGLRRGDEI LAVGQVPVND EAGFRKAMDK AGKNVPLLVM RRGNTLFIAL NLQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggcagct ttttcggtgc ggacaaaaaa gaagcatcct tcgtagaacg catcgaacac accaaagacg acggcagtgt cagtatgctg ctgcccgact ttgcccaact ggttcaaagc gaaggcccgg cagtcgtcaa tattcaggca gcccccgccc cgcgcaccca aaacggcagc ggcaatgccg aaaccgattc cgacccgctt gccgacagcg acccgttcta cgaatttttc aaacgcctcg tcccgaacat gcccgaaatc ccccaagaag aagcagatga cggcggattg aacttcggtt cgggcttcat catcagcaaa aacggctaca tcctgaccaa tacccacgtc gttgccggta tgggcagtat caaagtcctg ctcaacgaca agcgcgaata taccgccaaa ctcatcggtt cggatgtcca atccgatgtc gcccttctga aaatcgacgc aacggaagag ctacccgtcg tcaaaatcgg caatcccaaa aatttgaaac cgggcgaatg ggtcgctgcc atcggcgcgc ccttcggctt tgacaacagc gtgaccgccg gcatcgtgtc cgccaaaggc agaagcctgc ccaacgaaag ctacacaccc ttcatccaaa ccgacgttgc catcaatccg ggcaattccg gcggcccgct gttcaactta aaaggacagg tcgtcggcat caattcgcaa atatacagcc gcagcggcgg attcatgggc atctcctttg ccatcccgat tgacgttgcc atgaatgtcg ccgaacagct gaaaaacacc ggcaaagtcc aacgcggaca actgggcgtg attattcagg aagtatccta cggtttggca cagtcgttcg gtctggataa agccagcggc gcattgattg ccaaaatcct tcccggcagc cccgcagaac gtgccggcct gcaggcgggc gacatcgtcc tcagcctcga cggcggagaa atacgttctt ccggcgacct tcccgtcatg gtcggcgcca ttacgccggg aaaagaagtc agcctcggcg tatggcgcaa aggcgaagaa atcacaatca aagccaagct gggcaacgcc gccgagcata ccggcgcatc atccaaaaca gatgaagccc cctacaccga acagcaatcc ggtacgttct cggtcgaatc cgcaggcatt acccttcaga cacataccga cagcagcggc aaacacctcg tcgtcgtacg ggtttccgac gcggcagaac gcgcaggctt aaggcgcggc gacgaaatcc tcgccgtcgg gcaagtcccc gtcaatgacg aagccggttt ccgcaaagct atggacaagg caggcaaaaa cgtccccctg ctggtcatgc gccgtggcaa cacgctgttc atcgcattaa acctgcaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat