NegoA.01623.a
inorganic pyrophosphatase
| CENTER ID: | NegoA.01623.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.01623.a.B1.GE40055 | full length | 1 | 177 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5TEA
DEPOSITED: 9/20/2016
DETERMINATION: XRay
CLONE: NegoA.01623.a.B1.GE40055
PROTEIN: NegoA.01623.a.B1.PS37944
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.396 |
| UniProt: | B4RJP8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADFNQILTP GDVDGGIINV VNEIPAGSNH KIEWNRKLAA FQLDRIEPAI FAKPTNYGFI PQTLDEDGDE LDVLLVTEQP LATGVFLEAR VIGVMKFVDD GEVDDKIVCV PADDRNNGNA YKTLSDLPQQ LIKQIEFHFN HYKDLKKAGT TKVESWGGAE EAKKVIKESI ERWNKQA
NT Sequence
ATGGCAGACT TCAACCAAAT CCTGACCCCG GGCGACGTGG ACGGCGGCAT CATCAACGTC GTCAACGAAA TCCCCGCCGG CAGCAACCAC AAAATCGAGT GGAACCGCAA ACTCGCCGCA TTCCAACTCG ACCGCATCGA ACCCGCCATC TTCGCCAAAC CGACCAACTA CGGCTTCATC CCCCAAACTT TGGACGAAGA CGGCGATGAA TTGGACGTGT TGCTCGTTAC CGAACAACCT CTGGCAACCG GCGTATTCTT GGAAGCGCGC GTTATCGGCG TGATGAAATT CGTCGACGAC GGCGAAGTGG ACGACAAAAT CGTCTGCGTA CCTGCCGACG ACCGCAATAA CGGCAACGCC TACAAAACTT TGTCCGATTT GCCGCAACAG CTCATCAAAC AAATCGAGTT CCACTTCAAC CACTACAAAG ACCTGAAAAA AGCGGGTACG ACCAAAGTCG AATCCTGGGG CGGTGCGGAA GAAGCGAAAA AAGTCATCAA AGAATCCATC GAACGTTGGA ACAAACAGGC ATAA
Details for NegoA.01623.a.B1.GE40055
| HARVESTED ON: | 3/24/2016 |
| SEQUENCED ON: | 3/25/2016 |
| EXPECTED MW: | 21kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA DFNQILTPGD VDGGIINVVN EIPAGSNHKI EWNRKLAAFQ LDRIEPAIFA KPTNYGFIPQ TLDEDGDELD VLLVTEQPLA TGVFLEARVI GVMKFVDDGE VDDKIVCVPA DDRNNGNAYK TLSDLPQQLI KQIEFHFNHY KDLKKAGTTK VESWGGAEEA KKVIKESIER WNKQA
Validated NT Sequence
atcttactca cttatgcctg tttgttccaa cgttcgatgg attctttgat gacttttttc gcttcttccg caccgcccca ggattcgact ttggtcgtac ccgctttttt caggtctttg tagtggttga agtggaactc gatttgtttg atgagctgtt gcggcaaatc ggacaaagtt ttgtaggcgt tgccgttatt gcggtcgtcg gcaggtacgc agacgatttt gtcgtccact tcgccgtcgt cgacgaattt catcacgccg ataacgcgcg cttccaagaa tacgccggtt gccagaggtt gttcggtaac gagcaacacg tccaattcat cgccgtcttc gtccaaagtt tgggggatga agccgtagtt ggtcggtttg gcgaagatgg cgggttcgat gcggtcgagt tggaatgcgg cgagtttgcg gttccactcg attttgtggt tgctgccggc ggggatttcg ttgacgacgt tgatgatgcc gccgtccacg tcgcccgggg tcaggatttg gttgaagtct gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMA DFNQILTPGD VDGGIINVVN EIPAGSNHKI EWNRKLAAFQ LDRIEPAIFA KPTNYGFIPQ TLDEDGDELD VLLVTEQPLA TGVFLEARVI GVMKFVDDGE VDDKIVCVPA DDRNNGNAYK TLSDLPQQLI KQIEFHFNHY KDLKKAGTTK VESWGGAEEA KKVIKESIER WNKQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcag acttcaacca aatcctgacc ccgggcgacg tggacggcgg catcatcaac gtcgtcaacg aaatccccgc cggcagcaac cacaaaatcg agtggaaccg caaactcgcc gcattccaac tcgaccgcat cgaacccgcc atcttcgcca aaccgaccaa ctacggcttc atcccccaaa ctttggacga agacggcgat gaattggacg tgttgctcgt taccgaacaa cctctggcaa ccggcgtatt cttggaagcg cgcgttatcg gcgtgatgaa attcgtcgac gacggcgaag tggacgacaa aatcgtctgc gtacctgccg acgaccgcaa taacggcaac gcctacaaaa ctttgtccga tttgccgcaa cagctcatca aacaaatcga gttccacttc aaccactaca aagacctgaa aaaagcgggt acgaccaaag tcgaatcctg gggcggtgcg gaagaagcga aaaaagtcat caaagaatcc atcgaacgtt ggaacaaaca ggcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat