NegoA.01561.a

glucose-6-phosphate dehydrogenase

CENTER ID: NegoA.01561.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.01561.a.B1.GE40053 full length 1 481
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.1258
UniProt: B4RM14

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTQTNFDLV LFGATGDLAM RKLLPCLYQA HVAGLLHPEG RILGVSRSEL DTEGFLAKVE TSSKIHVKEN FSGEAWASFV ERLAYLKVDV TQPDDFAALG DLVKARKETD NVVIYLSTAP KFFAQACENL AAIGLNADNV RVVLEKPLGT DLASSQQINT DVARYFKEGQ IYRIDHYLGK ESLQNLLALR FANVMFEPLW NNKYIESVQL TIAEQLGVEE RGEFYDITGA LRDMVQNHLM QMLCMTAMEA PAGLDADAVR DEKVKVIKSL KPLTIESVNE NVVRGQYTAA KGMNGYLEEI NVPQDSFTET YVAIKAEIEN ERWKGVPFYL RTGKRMAGKV AEIVLNFRPL QNHIFDNSQT APNRLVIELQ PNESVRLYTQ VKTPGAGNKV EVTPLGVDLG KAVEGRRAEA YERLLLDVIN GKLALFNRRD ELEAAWEYVM PILENWANNT TPPHGYGAHS WGPEAARELL ARDGNKWHEE Q
NT Sequence
ATGAGTACAC AGACAAATTT TGATTTGGTG TTGTTCGGTG CGACCGGCGA TTTGGCAATG CGCAAACTTT TGCCCTGCCT GTATCAGGCG CATGTTGCCG GTCTGCTTCA TCCCGAAGGG CGTATTTTGG GGGTCAGCCG CAGCGAGTTG GATACCGAAG GTTTTCTGGC GAAGGTGGAA ACCAGTTCCA AAATCCATGT GAAAGAAAAT TTTTCCGGTG AGGCGTGGGC TTCGTTTGTT GAACGTCTCG CATATCTCAA AGTCGATGTT ACGCAGCCGG ACGATTTTGC CGCTTTGGGC GATTTGGTCA AAGCACGCAA AGAAACCGAC AACGTCGTCA TCTATCTGTC CACCGCGCCG AAATTCTTCG CGCAAGCCTG CGAAAACCTT GCTGCGATCG GTTTGAATGC CGACAATGTG CGCGTGGTTT TGGAAAAACC GCTGGGTACG GATTTGGCTT CTTCCCAACA AATCAATACC GATGTGGCGC GTTATTTCAA AGAAGGGCAA ATTTACCGCA TCGACCACTA CCTTGGCAAA GAAAGCCTGC AAAACCTGCT TGCGCTGCGT TTTGCCAATG TAATGTTCGA GCCGCTGTGG AACAATAAAT ACATTGAAAG CGTGCAGCTG ACCATCGCCG AACAGCTCGG TGTGGAAGAG CGCGGCGAGT TTTACGACAT TACCGGCGCG TTGCGCGATA TGGTGCAAAA CCATTTGATG CAGATGCTGT GCATGACTGC GATGGAAGCC CCCGCCGGCT TGGATGCCGA CGCGGTGCGC GATGAAAAAG TCAAAGTCAT CAAGTCATTG AAGCCGCTGA CCATCGAATC TGTCAATGAA AATGTCGTGC GCGGACAATA TACCGCTGCC AAAGGCATGA ACGGCTATCT TGAAGAAATC AACGTTCCGC AAGACAGCTT TACCGAAACC TACGTCGCCA TTAAAGCCGA AATCGAAAAC GAACGCTGGA AGGGCGTTCC CTTCTACCTG CGTACCGGCA AACGCATGGC GGGCAAAGTG GCGGAAATCG TTTTGAACTT CCGCCCGCTG CAAAACCATA TTTTCGACAA CAGCCAAACC GCGCCGAACC GTTTGGTTAT CGAGCTTCAA CCGAATGAAT CCGTCCGCCT TTACACGCAG GTGAAAACCC CGGGTGCGGG CAACAAAGTG GAAGTAACGC CTTTGGGCGT GGATTTGGGC AAGGCCGTTG AAGGCCGCCG CGCCGAGGCT TACGAACGCC TGCTGTTGGA TGTGATTAAC GGCAAACTCG CTTTGTTCAA CCGCCGCGAC GAACTCGAAG CCGCGTGGGA ATATGTGATG CCGATTTTGG AAAACTGGGC AAATAACACC ACGCCGCCGC ACGGTTACGG CGCACACTCT TGGGGTCCTG AAGCCGCCCG CGAACTGTTG GCGCGCGACG GCAACAAGTG GCACGAAGAA CAGTAA
Details for NegoA.01561.a.B1.GE40053
HARVESTED ON: data unavailable
SEQUENCED ON: 3/25/2016
EXPECTED MW: 55kDa
OBSERVED MW: 8kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS TQTNFDLVLF GATGDLAMRK LLPCLYQAHV AGLLHPEGRI LGVSRXELDT EGFLAKVETS SKIHVKENFS GEAWASFVER LAYLKVDVTQ PXXFAALGDL VKARKETDNV XIYLSTAPKF FAQACENLAA IGLNADNVRV VLEKPLGTDL ASSQQINTDV ARYFKEGQIY RIDHYLGKES LQNLLALRFA NVMFEPLWNN KYIESVQLTI AEQLGVEERG EXXDITGALR DMVQNHLMQM LCMTAMEAPA GLDADAVRDE KVKVIKSLKP LTXXSVNENV VRGQYTAAKG MNGYLEEINV PQDSFTETYV AIKAEIENER WKGVPFYLRT GKRMAGKVAE IVLNFRPLQN HIFDNSQTAP NRLVIELQPN ESVRLYTQVK TPGAGNKVEV TPLGVDLGKA VEGRRAEAYE RLLLDVINGK LALFNRRDEL EAAWEYVMPI LENWANNTTP PHGYGAHSWG XEAARELLAR
Validated NT Sequence
cgcgcgccaa cagttcgcgg gcggcttcan naccccaaga gtgtgcgccg taaccgtgcg gcggcgtggt gttatttgcc cagttttcca aaatcggcat cacatattcc cacgcggctt cgagttcgtc gcggcggttg aacaaagcga gtttgccgtt aatcacatcc aacagcaggc gttcgtaagc ctcggcgcgg cggccttcaa cggccttgcc caaatccacg cccaaaggcg ttacttccac tttgttgccc gcacccgggg ttttcacctg cgtgtaaagg cggacggatt cattcggttg aagctcgata accaaacggt tcggcgcggt ttggctgttg tcgaaaatat ggttttgcag cgggcggaag ttcaaaacga tttccgccac tttgcccgcc atgcgtttgc cggtacgcag gtagaaggga acgcccttcc agcgttcgtt ttcgatttcg gctttaatgg cgacgtaggt ttcggtaaag ctgtcttgcg gaacgttgat ttcttcaaga tagccgttca tgcctttggc agcggtatat tgtccgcgca cgacattttc attgacagat tnnntggtca gcggcttcaa tgacttgatg actttgactt tttcatcgcg caccgcgtcg gcatccaagc cggcgggggc ttccatcgca gtcatgcaca gcatctgcat caaatggttt tgcaccatat cgcgcaacgc gccggtaatg tcgtnaanct cgccgcgctc ttccacaccg agctgttcgg cgatggtcag ctgcacgctt tcgatgtatt tattgttcca cagcggctcg aacattacat tggcaaaacg cagcgcaagc aggttttgca ggctttcttt gccaaggtag tggtcgatgc ggtaaatttg cccttctttg aaataacgcg ccacatcggt attgatttgt tgggaagaag ccaaatccgt acccagcggt ttttccaaaa ccacgcgcac attgtcggca ttcaaaccga tcgcagcaag gttttcgcag gcttgcgcga agaatttcgg cgcggtggac agatagatga nnacgttgtc ggtttctttg cgtgctttga ccaaatcgcc caaagcggca aaatnntccg gctgcgtaac atcgactttg agatatgcga gacgttcaac aaacgaagcc cacgcctcac cggaaaaatt ttctttcaca tggattttgg aactggtttc caccttcgcc agaaaacctt cggtatccaa ctcnnngcgg ctgaccccca aaatacgccc ttcgggatga agcagaccgg caacatgcgc ctgatacagg cagggcaaaa gtttgcgcat tgccaaatcg ccggtcgcac cgaacaacac caaatcaaaa tttgtctgtg tactcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS TQTNFDLVLF GATGDLAMRK LLPCLYQAHV AGLLHPEGRI LGVSRSELDT EGFLAKVETS SKIHVKENFS GEAWASFVER LAYLKVDVTQ PDDFAALGDL VKARKETDNV VIYLSTAPKF FAQACENLAA IGLNADNVRV VLEKPLGTDL ASSQQINTDV ARYFKEGQIY RIDHYLGKES LQNLLALRFA NVMFEPLWNN KYIESVQLTI AEQLGVEERG EFYDITGALR DMVQNHLMQM LCMTAMEAPA GLDADAVRDE KVKVIKSLKP LTIESVNENV VRGQYTAAKG MNGYLEEINV PQDSFTETYV AIKAEIENER WKGVPFYLRT GKRMAGKVAE IVLNFRPLQN HIFDNSQTAP NRLVIELQPN ESVRLYTQVK TPGAGNKVEV TPLGVDLGKA VEGRRAEAYE RLLLDVINGK LALFNRRDEL EAAWEYVMPI LENWANNTTP PHGYGAHSWG PEAARELLAR DGNKWHEEQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta cacagacaaa ttttgatttg gtgttgttcg gtgcgaccgg cgatttggca atgcgcaaac ttttgccctg cctgtatcag gcgcatgttg ccggtctgct tcatcccgaa gggcgtattt tgggggtcag ccgcagcgag ttggataccg aaggttttct ggcgaaggtg gaaaccagtt ccaaaatcca tgtgaaagaa aatttttccg gtgaggcgtg ggcttcgttt gttgaacgtc tcgcatatct caaagtcgat gttacgcagc cggacgattt tgccgctttg ggcgatttgg tcaaagcacg caaagaaacc gacaacgtcg tcatctatct gtccaccgcg ccgaaattct tcgcgcaagc ctgcgaaaac cttgctgcga tcggtttgaa tgccgacaat gtgcgcgtgg ttttggaaaa accgctgggt acggatttgg cttcttccca acaaatcaat accgatgtgg cgcgttattt caaagaaggg caaatttacc gcatcgacca ctaccttggc aaagaaagcc tgcaaaacct gcttgcgctg cgttttgcca atgtaatgtt cgagccgctg tggaacaata aatacattga aagcgtgcag ctgaccatcg ccgaacagct cggtgtggaa gagcgcggcg agttttacga cattaccggc gcgttgcgcg atatggtgca aaaccatttg atgcagatgc tgtgcatgac tgcgatggaa gcccccgccg gcttggatgc cgacgcggtg cgcgatgaaa aagtcaaagt catcaagtca ttgaagccgc tgaccatcga atctgtcaat gaaaatgtcg tgcgcggaca atataccgct gccaaaggca tgaacggcta tcttgaagaa atcaacgttc cgcaagacag ctttaccgaa acctacgtcg ccattaaagc cgaaatcgaa aacgaacgct ggaagggcgt tcccttctac ctgcgtaccg gcaaacgcat ggcgggcaaa gtggcggaaa tcgttttgaa cttccgcccg ctgcaaaacc atattttcga caacagccaa accgcgccga accgtttggt tatcgagctt caaccgaatg aatccgtccg cctttacacg caggtgaaaa ccccgggtgc gggcaacaaa gtggaagtaa cgcctttggg cgtggatttg ggcaaggccg ttgaaggccg ccgcgccgag gcttacgaac gcctgctgtt ggatgtgatt aacggcaaac tcgctttgtt caaccgccgc gacgaactcg aagccgcgtg ggaatatgtg atgccgattt tggaaaactg ggcaaataac accacgccgc cgcacggtta cggcgcacac tcttggggtc ctgaagccgc ccgcgaactg ttggcgcgcg acggcaacaa gtggcacgaa gaacagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t