SSGCID: Target Page

NegoA.01474.a

50S ribosomal protein L10

CENTER ID:	NegoA.01474.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.01474.a.B1.GE40052	full length		1	166

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLNIETKKV AVEEISAAIA NAQTLVVAEY RGISVSSMTE LRANARKEGV YLRVLKNTLA RRAVQGTSFV ELADQMVGPL VYAASEDAVA AAKVLHQFAK KDDKIVVKAG SYNGEVMNAA QVAELASIPS REELLSKLLF VMQAPVSGFA RSLAALAEKK AGEEAA

NT Sequence

TTGAGTCTCA ATATTGAAAC CAAGAAAGTG GCGGTCGAGG AAATTAGCGC GGCAATTGCT AATGCTCAAA CCCTCGTAGT CGCTGAATAT CGCGGTATCA GTGTTTCCAG TATGACTGAG CTTCGTGCGA ATGCACGTAA AGAAGGCGTT TATTTGCGCG TTCTGAAAAA TACTCTGGCT CGTCGCGCAG TGCAAGGTAC TTCATTTGTA GAATTGGCCG ATCAAATGGT TGGTCCGTTG GTTTACGCTG CTTCTGAAGA TGCTGTTGCT GCTGCTAAAG TGTTGCACCA ATTCGCGAAA AAAGATGACA AAATTGTCGT TAAAGCCGGT TCTTACAATG GCGAAGTAAT GAATGCTGCT CAGGTTGCTG AGTTGGCTTC TATTCCGAGC CGCGAAGAGC TGTTGTCCAA ACTGTTGTTC GTTATGCAAG CTCCTGTATC GGGCTTTGCG CGCAGTTTGG CTGCTTTGGC AGAGAAAAAA GCCGGCGAAG AAGCTGCTTA A

Details for NegoA.01474.a.B1.GE40052

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	19kDa
OBSERVED MW:	17kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML SLNIETKKVA VEEISAAIAN AQTLVVAEYR GISVSSMTEL RANARKEGVY LRVLKNTLAR RAVQGTSFVE LADQMVGPLV YAASEDAVAA AKVLHQFAKK DDKIVVKAGS YNGEVMNAAQ VAELASIPSR EELLSKLLFV MQAPVSGFAR GLAALAEKKA GEEAA

Validated NT Sequence

atcttactca cttaagcagc ttcttcgccg gcttttttct ctgccaaagc agccaaaccg cgcgcaaagc ccgatacagg agcttgcata acgaacaaca gtttggacaa cagctcttcg cggctcggaa tagaagccaa ctcagcaacc tgagcagcat tcattacttc gccattgtaa gaaccggctt taacgacaat tttgtcatct tttttcgcga attggtgcaa cactttagca gcagcaacag catcttcaga agcagcgtaa accaacggac caaccatttg atcggccaat tctacaaatg aagtaccttg cactgcgcga cgagccagag tatttttcag aacgcgcaaa taaacgcctt ctttacgtgc attcgcacga agctcagtca tactggaaac actgataccg cgatattcag cgactacgag ggtttgagca ttagcaattg ccgcgctaat ttcctcgacc gccactttct tggtttcaat attgagactc aacatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMS LNIETKKVAV EEISAAIANA QTLVVAEYRG ISVSSMTELR ANARKEGVYL RVLKNTLARR AVQGTSFVEL ADQMVGPLVY AASEDAVAAA KVLHQFAKKD DKIVVKAGSY NGEVMNAAQV AELASIPSRE ELLSKLLFVM QAPVSGFARS LAALAEKKAG EEAA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgagtc tcaatattga aaccaagaaa gtggcggtcg aggaaattag cgcggcaatt gctaatgctc aaaccctcgt agtcgctgaa tatcgcggta tcagtgtttc cagtatgact gagcttcgtg cgaatgcacg taaagaaggc gtttatttgc gcgttctgaa aaatactctg gctcgtcgcg cagtgcaagg tacttcattt gtagaattgg ccgatcaaat ggttggtccg ttggtttacg ctgcttctga agatgctgtt gctgctgcta aagtgttgca ccaattcgcg aaaaaagatg acaaaattgt cgttaaagcc ggttcttaca atggcgaagt aatgaatgct gctcaggttg ctgagttggc ttctattccg agccgcgaag agctgttgtc caaactgttg ttcgttatgc aagctcctgt atcgggcttt gcgcgcagtt tggctgcttt ggcagagaaa aaagccggcg aagaagctgc ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat