NegoA.01416.a
fructose-bisphosphate aldolase
| CENTER ID: | NegoA.01416.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.01416.a.B1.GE40051 | full length | 1 | 354 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.01416.a.B1.PS37962 | full length | 1 | 354 |
Structures
5U4N
DEPOSITED: 12/5/2016
DETERMINATION: XRay
CLONE: NegoA.01416.a.B1.GE40051
PROTEIN: NegoA.01416.a.B1.PS37962
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.52 |
| UniProt: | B4RNW4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALVSMRQLL DHAAENSYGL PAFNVNNLEQ MRAIMEAADQ VNAPVIVQAS AGARKYAGAP FLRHLILAAV EEFPHIPVVM HQDHGASPDV CQRSIQLGFS SVMMDGSLLE DGKTPSSYEY NVNATRTVVN FSHACGVSVE GEIGVLGNLE TGEAGEEDGV GAAGKLSHDQ MLTSVEDAVR FVKDTGVDAL AIAVGTSHGA YKFTRPPTGD VLRIDRIKEI HQALPNTHIV MHGSSSVPQE WLKVINEYGG NIGETYGVPV EEIVEGIKHG VRKVNIDTDL RLASTGAVRR YLAENPSDFD PRKYLGKTIE AMKQICLDRY LAFGCEGQAG KIKPVSLEKM ASRYAKGELN QIVK
NT Sequence
ATGGCACTCG TATCCATGCG CCAACTGCTC GACCACGCCG CCGAAAACAG CTACGGCCTG CCCGCGTTCA ACGTCAACAA CCTCGAACAA ATGCGCGCCA TTATGGAAGC CGCCGACCAA GTCAACGCGC CCGTCATCGT ACAGGCGAGC GCAGGTGCGC GCAAATACGC GGGCGCGCCG TTTTTGCGCC ACCTGATTCT GGCGGCAGTC GAAGAATTTC CGCACATCCC CGTCGTGATG CACCAAGACC ACGGCGCATC GCCCGACGTG TGCCAACGCT CCATCCAACT GGGCTTCTCC TCCGTGATGA TGGACGGCTC TTTGCTCGAA GACGGCAAAA CCCCTTCTTC TTACGAATAC AACGTCAACG CCACCCGTAC CGTCGTCAAC TTCTCCCACG CCTGCGGCGT GTCCGTCGAA GGCGAAATCG GCGTATTGGG CAACCTCGAA ACCGGCGAAG CAGGCGAAGA AGACGGAGTG GGCGCGGCAG GCAAACTCTC ACACGACCAA ATGCTCACCA GCGTTGAAGA TGCCGTGCGT TTCGTTAAAG ATACCGGCGT TGACGCATTG GCGATTGCCG TCGGCACCAG CCACGGCGCA TACAAATTCA CCCGTCCGCC CACAGGCGAC GTATTGCGTA TCGACCGCAT CAAGGAAATC CACCAAGCCC TGCCCAATAC ACACATCGTG ATGCACGGCT CCAGCTCCGT TCCGCAAGAA TGGCTGAAAG TCATCAACGA ATACGGCGGC AATATCGGCG AAACCTACGG CGTGCCGGTT GAAGAAATCG TCGAAGGCAT CAAACACGGC GTGCGCAAAG TCAACATCGA TACCGACCTG CGCCTCGCTT CCACCGGCGC GGTACGCCGC TACCTTGCCG AAAACCCGTC CGACTTTGAT CCGCGCAAAT ACTTGGGCAA AACCATTGAA GCGATGAAGC AAATCTGCCT CGACCGTTAT CTTGCGTTCG GTTGCGAAGG TCAGGCAGGC AAAATCAAAC CTGTTTCGTT GGAAAAAATG GCAAGCCGTT ATGCCAAGGG CGAATTGAAC CAAATCGTCA AATAA
Details for NegoA.01416.a.B1.GE40051
| HARVESTED ON: | 3/24/2016 |
| SEQUENCED ON: | 3/25/2016 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMA LVSMRQLLDH AAENSYGLPA FNVNNLEQMR AIMEAADQVN APVIVQASAG ARKYAGAPFL RHLILAAVEE FPHIPVVMHQ DHGASPDVCQ RSIQLGFSSV MMDGSLLEDG KTPSSYEYNV NATRTVVNFS HACGVSVEGE IGVLGNLETG EAGEEDGVGA AGKLSHDQML TSVEDAVRFV KDTGVDALAI AVGTSHGAYK FTRPPTGDVL RIDRIKEIHQ ALPNTHIVMH GSSSVPQEWL KVINEYGGNI GETYGVPVEE IVEGIKHGVR KVNIDTDLRL ASTGAVRRYL AENPSDFDPR KYLGKTIEAM KQICLDRYLA FGCEGQAGKI KPVSLEKMAS RY
Validated NT Sequence
cataacggct tgccattttt tccaacgaaa caggtttgat tttgcctgcc tgaccttcgc aaccgaacgc aagataacgg tcgaggcaga tttgcttcat cgcttcaatg gttttgccca agtatttgcg cggatcaaag tcggacgggt tttcggcaag gtagcggcgt accgcgccgg tggaagcgag gcgcaggtcg gtatcgatgt tgactttgcg cacgccgtgt ttgatgcctt cgacgatttc ttcaaccggc acgccgtagg tttcgccgat attgccgccg tattcgttga tgactttcag ccattcttgc ggaacggagc tggagccgtg catcacgatg tgtgtattgg gcagggcttg gtggatttcc ttgatgcggt cgatacgcaa tacgtcgcct gtgggcggac gggtgaattt gtatgcgccg tggctggtgc cgacggcaat cgccaatgcg tcaacgccgg tatctttaac gaaacgcacg gcatcttcaa cgctggtgag catttggtcg tgtgagagtt tgcctgccgc gcccactccg tcttcttcgc ctgcttcgcc ggtttcgagg ttgcccaata cgccgatttc gccttcgacg gacacgccgc aggcgtggga gaagttgacg acggtacggg tggcgttgac gttgtattcg taagaagaag gggttttgcc gtcttcgagc aaagagccgt ccatcatcac ggaggagaag cccagttgga tggagcgttg gcacacgtcg ggcgatgcgc cgtggtcttg gtgcatcacg acggggatgt gcggaaattc ttcgactgcc gccagaatca ggtggcgcaa aaacggcgcg cccgcgtatt tgcgcgcacc tgcgctcgcc tgtacgatga cgggcgcgtt gacttggtcg gcggcttcca taatggcgcg catttgttcg aggttgttga cgttgaacgc gggcaggccg tagctgtttt cggcggcgtg gtcaagcagt tggcgcatgg atacgagtgc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMA LVSMRQLLDH AAENSYGLPA FNVNNLEQMR AIMEAADQVN APVIVQASAG ARKYAGAPFL RHLILAAVEE FPHIPVVMHQ DHGASPDVCQ RSIQLGFSSV MMDGSLLEDG KTPSSYEYNV NATRTVVNFS HACGVSVEGE IGVLGNLETG EAGEEDGVGA AGKLSHDQML TSVEDAVRFV KDTGVDALAI AVGTSHGAYK FTRPPTGDVL RIDRIKEIHQ ALPNTHIVMH GSSSVPQEWL KVINEYGGNI GETYGVPVEE IVEGIKHGVR KVNIDTDLRL ASTGAVRRYL AENPSDFDPR KYLGKTIEAM KQICLDRYLA FGCEGQAGKI KPVSLEKMAS RYAKGELNQI VK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcac tcgtatccat gcgccaactg ctcgaccacg ccgccgaaaa cagctacggc ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac caagtcaacg cgcccgtcat cgtacaggcg agcgcaggtg cgcgcaaata cgcgggcgcg ccgtttttgc gccacctgat tctggcggca gtcgaagaat ttccgcacat ccccgtcgtg atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttc tcctccgtga tgatggacgg ctctttgctc gaagacggca aaaccccttc ttcttacgaa tacaacgtca acgccacccg taccgtcgtc aacttctccc acgcctgcgg cgtgtccgtc gaaggcgaaa tcggcgtatt gggcaacctc gaaaccggcg aagcaggcga agaagacgga gtgggcgcgg caggcaaact ctcacacgac caaatgctca ccagcgttga agatgccgtg cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc gcatacaaat tcacccgtcc gcccacaggc gacgtattgc gtatcgaccg catcaaggaa atccaccaag ccctgcccaa tacacacatc gtgatgcacg gctccagctc cgttccgcaa gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgataccgac ctgcgcctcg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttt gatccgcgca aatacttggg caaaaccatt gaagcgatga agcaaatctg cctcgaccgt tatcttgcgt tcggttgcga aggtcaggca ggcaaaatca aacctgtttc gttggaaaaa atggcaagcc gttatgccaa gggcgaattg aaccaaatcg tcaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for NegoA.01416.a.B1.PS37962
| PURIFICATION DATe: | 6/30/2016 |
| CONCENTRATION: | 63.56mg/ml |
| OBSERVED MW: | 39kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA LVSMRQLLDH AAENSYGLPA FNVNNLEQMR AIMEAADQVN APVIVQASAG ARKYAGAPFL RHLILAAVEE FPHIPVVMHQ DHGASPDVCQ RSIQLGFSSV MMDGSLLEDG KTPSSYEYNV NATRTVVNFS HACGVSVEGE IGVLGNLETG EAGEEDGVGA AGKLSHDQML TSVEDAVRFV KDTGVDALAI AVGTSHGAYK FTRPPTGDVL RIDRIKEIHQ ALPNTHIVMH GSSSVPQEWL KVINEYGGNI GETYGVPVEE IVEGIKHGVR KVNIDTDLRL ASTGAVRRYL AENPSDFDPR KYLGKTIEAM KQICLDRYLA FGCEGQAGKI KPVSLEKMAS RY
Validated NT Sequence
cataacggct tgccattttt tccaacgaaa caggtttgat tttgcctgcc tgaccttcgc aaccgaacgc aagataacgg tcgaggcaga tttgcttcat cgcttcaatg gttttgccca agtatttgcg cggatcaaag tcggacgggt tttcggcaag gtagcggcgt accgcgccgg tggaagcgag gcgcaggtcg gtatcgatgt tgactttgcg cacgccgtgt ttgatgcctt cgacgatttc ttcaaccggc acgccgtagg tttcgccgat attgccgccg tattcgttga tgactttcag ccattcttgc ggaacggagc tggagccgtg catcacgatg tgtgtattgg gcagggcttg gtggatttcc ttgatgcggt cgatacgcaa tacgtcgcct gtgggcggac gggtgaattt gtatgcgccg tggctggtgc cgacggcaat cgccaatgcg tcaacgccgg tatctttaac gaaacgcacg gcatcttcaa cgctggtgag catttggtcg tgtgagagtt tgcctgccgc gcccactccg tcttcttcgc ctgcttcgcc ggtttcgagg ttgcccaata cgccgatttc gccttcgacg gacacgccgc aggcgtggga gaagttgacg acggtacggg tggcgttgac gttgtattcg taagaagaag gggttttgcc gtcttcgagc aaagagccgt ccatcatcac ggaggagaag cccagttgga tggagcgttg gcacacgtcg ggcgatgcgc cgtggtcttg gtgcatcacg acggggatgt gcggaaattc ttcgactgcc gccagaatca ggtggcgcaa aaacggcgcg cccgcgtatt tgcgcgcacc tgcgctcgcc tgtacgatga cgggcgcgtt gacttggtcg gcggcttcca taatggcgcg catttgttcg aggttgttga cgttgaacgc gggcaggccg tagctgtttt cggcggcgtg gtcaagcagt tggcgcatgg atacgagtgc catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMA LVSMRQLLDH AAENSYGLPA FNVNNLEQMR AIMEAADQVN APVIVQASAG ARKYAGAPFL RHLILAAVEE FPHIPVVMHQ DHGASPDVCQ RSIQLGFSSV MMDGSLLEDG KTPSSYEYNV NATRTVVNFS HACGVSVEGE IGVLGNLETG EAGEEDGVGA AGKLSHDQML TSVEDAVRFV KDTGVDALAI AVGTSHGAYK FTRPPTGDVL RIDRIKEIHQ ALPNTHIVMH GSSSVPQEWL KVINEYGGNI GETYGVPVEE IVEGIKHGVR KVNIDTDLRL ASTGAVRRYL AENPSDFDPR KYLGKTIEAM KQICLDRYLA FGCEGQAGKI KPVSLEKMAS RYAKGELNQI VK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAC TCGTATCCAT GCGCCAACTG CTCGACCACG CCGCCGAAAA CAGCTACGGC CTGCCCGCGT TCAACGTCAA CAACCTCGAA CAAATGCGCG CCATTATGGA AGCCGCCGAC CAAGTCAACG CGCCCGTCAT CGTACAGGCG AGCGCAGGTG CGCGCAAATA CGCGGGCGCG CCGTTTTTGC GCCACCTGAT TCTGGCGGCA GTCGAAGAAT TTCCGCACAT CCCCGTCGTG ATGCACCAAG ACCACGGCGC ATCGCCCGAC GTGTGCCAAC GCTCCATCCA ACTGGGCTTC TCCTCCGTGA TGATGGACGG CTCTTTGCTC GAAGACGGCA AAACCCCTTC TTCTTACGAA TACAACGTCA ACGCCACCCG TACCGTCGTC AACTTCTCCC ACGCCTGCGG CGTGTCCGTC GAAGGCGAAA TCGGCGTATT GGGCAACCTC GAAACCGGCG AAGCAGGCGA AGAAGACGGA GTGGGCGCGG CAGGCAAACT CTCACACGAC CAAATGCTCA CCAGCGTTGA AGATGCCGTG CGTTTCGTTA AAGATACCGG CGTTGACGCA TTGGCGATTG CCGTCGGCAC CAGCCACGGC GCATACAAAT TCACCCGTCC GCCCACAGGC GACGTATTGC GTATCGACCG CATCAAGGAA ATCCACCAAG CCCTGCCCAA TACACACATC GTGATGCACG GCTCCAGCTC CGTTCCGCAA GAATGGCTGA AAGTCATCAA CGAATACGGC GGCAATATCG GCGAAACCTA CGGCGTGCCG GTTGAAGAAA TCGTCGAAGG CATCAAACAC GGCGTGCGCA AAGTCAACAT CGATACCGAC CTGCGCCTCG CTTCCACCGG CGCGGTACGC CGCTACCTTG CCGAAAACCC GTCCGACTTT GATCCGCGCA AATACTTGGG CAAAACCATT GAAGCGATGA AGCAAATCTG CCTCGACCGT TATCTTGCGT TCGGTTGCGA AGGTCAGGCA GGCAAAATCA AACCTGTTTC GTTGGAAAAA ATGGCAAGCC GTTATGCCAA GGGCGAATTG AACCAAATCG TCAAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT