SSGCID: Target Page

NegoA.01328.a

3-deoxy-manno-octulosonate cytidylyltransferase

CENTER ID:	NegoA.01328.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.01328.a.B1.GE40050	full length		1	253

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.425
UniProt:	B4RJR9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTEFVVLIPA RLDSSRLPGK ALADIHGKPM VVRVAEQAAK SKAARVVVAT DHPDIQTACQ AHGIEVVMTS NRHESGTTRL AEAAAALKLP PHLIVVNVQG DEPLIAPELI DRTAEVLVEN NVQMATAGHE LHDFDELMNP NAVKVVLDKN GNAIYFSRAP IPYPRDAMRA GKREMPSETA VLRHIGIYAY RVGFLQRYAE MSVSPLETIE SLEQLRVLWH GYPIAVETAK EAPAAGVDTQ EDLDRVRAVF QTV

NT Sequence

ATGACCGAAT TCGTCGTATT GATTCCGGCG CGGCTGGATT CGTCGCGCCT GCCCGGAAAA GCCTTGGCGG ACATTCACGG CAAACCGATG GTCGTGCGCG TTGCCGAACA GGCGGCAAAA AGCAAAGCCG CGCGCGTCGT CGTCGCCACC GATCATCCCG ACATTCAGAC GGCCTGTCAG GCGCACGGTA TCGAAGTCGT CATGACTTCA AACCGGCACG AAAGCGGCAC GACGCGCCTT GCCGAAGCCG CCGCCGCGCT GAAGCTGCCG CCGCATTTGA TTGTTGTGAA CGTACAGGGC GACGAGCCGC TGATTGCCCC CGAACTCATC GACCGCACCG CCGAAGTACT CGTCGAAAAC AACGTCCAAA TGGCGACCGC CGGCCACGAA TTGCACGATT TCGACGAATT GATGAATCCC AATGCCGTCA AAGTCGTCCT CGACAAAAAC GGCAACGCCA TCTACTTCAG CCGTGCCCCG ATTCCCTATC CGCGCGATGC GATGCGTGCC GGAAAACGCG AAATGCCGTC TGAAACCGCC GTCCTACGCC ATATCGGCAT TTACGCCTAC CGCGTCGGCT TCCTGCAACG CTATGCCGAA ATGAGCGTCT CGCCGCTGGA AACCATCGAA TCGCTGGAAC AACTGCGCGT CCTGTGGCAC GGCTACCCCA TCGCCGTCGA AACCGCCAAA GAAGCCCCCG CCGCCGGTGT GGATACGCAG GAAGATTTGG ACAGGGTTCG CGCTGTATTT CAGACCGTAT AA

Details for NegoA.01328.a.B1.GE40050

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	29kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT EFVVLIPARL DSSRLPGKAL ADIHGKPMVV RVAEQAAKSK AARVVVATDH PDIQTACQAH GIEVVMTSNR HESGTTRLAE AAAALKLPPH LIVVNVQGDE PLIAPELIDR TAEVLVENNV QMATAGHELH DFDELMNPNA VKVVLDKNGN AIYFSRAPIP YPRDAMRAGK REMPSETAVL RHIGIYAYRV GFLQRYAEMS VSPLETIESL EQLRVLWHGY PIAVETAKEA PAAGVDTQED LDRVRAVFQT V

Validated NT Sequence

atcttactca cttatacggt ctgaaataca gcgcgaaccc tgtccaaatc ttcctgcgta tccacaccgg cggcgggggc ttctttggcg gtttcgacgg cgatggggta gccgtgccac aggacgcgca gttgttccag cgattcgatg gtttccagcg gcgagacgct catttcggca tagcgttgca ggaagccgac gcggtaggcg taaatgccga tatggcgtag gacggcggtt tcagacggca tttcgcgttt tccggcacgc atcgcatcgc gcggataggg aatcggggca cggctgaagt agatggcgtt gccgtttttg tcgaggacga ctttgacggc attgggattc atcaattcgt cgaaatcgtg caattcgtgg ccggcggtcg ccatttggac gttgttttcg acgagtactt cggcggtgcg gtcgatgagt tcgggggcaa tcagcggctc gtcgccctgt acgttcacaa caatcaaatg cggcggcagc ttcagcgcgg cggcggcttc ggcaaggcgc gtcgtgccgc tttcgtgccg gtttgaagtc atgacgactt cgataccgtg cgcctgacag gccgtctgaa tgtcgggatg atcggtggcg acgacgacgc gcgcggcttt gctttttgcc gcctgttcgg caacgcgcac gaccatcggt ttgccgtgaa tgtccgccaa ggcttttccg ggcaggcgcg acgaatccag ccgcgccgga atcaatacga cgaattcggt catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg aattcgtcgt attgattccg gcgcggctgg attcgtcgcg cctgcccgga aaagccttgg cggacattca cggcaaaccg atggtcgtgc gcgttgccga acaggcggca aaaagcaaag ccgcgcgcgt cgtcgtcgcc accgatcatc ccgacattca gacggcctgt caggcgcacg gtatcgaagt cgtcatgact tcaaaccggc acgaaagcgg cacgacgcgc cttgccgaag ccgccgccgc gctgaagctg ccgccgcatt tgattgttgt gaacgtacag ggcgacgagc cgctgattgc ccccgaactc atcgaccgca ccgccgaagt actcgtcgaa aacaacgtcc aaatggcgac cgccggccac gaattgcacg atttcgacga attgatgaat cccaatgccg tcaaagtcgt cctcgacaaa aacggcaacg ccatctactt cagccgtgcc ccgattccct atccgcgcga tgcgatgcgt gccggaaaac gcgaaatgcc gtctgaaacc gccgtcctac gccatatcgg catttacgcc taccgcgtcg gcttcctgca acgctatgcc gaaatgagcg tctcgccgct ggaaaccatc gaatcgctgg aacaactgcg cgtcctgtgg cacggctacc ccatcgccgt cgaaaccgcc aaagaagccc ccgccgccgg tgtggatacg caggaagatt tggacagggt tcgcgctgta tttcagaccg tatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat