NegoA.01300.a

riboflavin biosynthesis protein RibF

CENTER ID: NegoA.01300.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.01300.a.B1.GE40049 full length 1 306
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIRPGRHNA PDFPHGAAVT IGNFDGVHLG HKHILQKLRL EADARGLPVV AVIFEPQPKE FFAARTGKTP PCRISPLRTK LGLLEKTGCV DAAWVLRFDR NFSEISAQAF IDRLLRQTLN TRYLLVGDDF RFGAGREGCF ELLAQQPDMQ TERTPSVIVE DIRTSSTTVR QALSDGNLAY AKKLLGHDYV LGGRVVHGRK LGRTLNAPTA NIRLPGHRYA LGGVFVVEAD GAFGTRRGVA SFGFNPTVDG GCSQKLEVHL FDFQGDLYGQ RLNVRFLHKL RDEEKFDGME ELKRRIEADM EAAKCW
NT Sequence
ATGAAAATCA GGCCGGGGCG GCACAACGCG CCCGACTTTC CGCACGGGGC CGCCGTAACC ATAGGCAATT TCGACGGCGT GCACCTCGGA CACAAACACA TCCTCCAAAA ACTCCGCCTC GAAGCCGACG CACGCGGATT GCCCGTCGTT GCCGTCATTT TCGAGCCGCA ACCCAAAGAA TTTTTTGCCG CCCGCACAGG CAAAACCCCG CCGTGCCGTA TCAGCCCCCT GCGTACCAAA CTCGGGCTGC TCGAAAAAAC CGGTTGCGTC GATGCCGCCT GGGTTTTGCG TTTCGATCGG AATTTTTCCG AAATATCCGC GCAAGCATTT ATCGACCGCC TGCTGCGTCA AACCTTGAAT ACGCGCTATT TGCTCGTCGG CGACGATTTC CGTTTCGGTG CGGGGCGGGA AGGCTGTTTT GAACTTTTGG CACAACAGCC CGATATGCAA ACCGAGCGCA CGCCTTCCGT CATCGTCGAA GACATCCGCA CCAGCAGCAC CACCGTCCGC CAAGCCCTTT CAGACGGCAA CCTTGCCTAT GCGAAGAAAC TTTTGGGGCA CGACTACGTT TTGGGGGGCA GGGTGGTGCA CGGCAGAAAA CTCGGGCGCA CCTTAAACGC CCCGACCGCC AACATCCGAC TGCCCGGCCA CCGTTATGCC CTCGGCGGCG TGTTTGTCGT CGAAGCGGAC GGCGCATTCG GCACGCGGCG CGGCGTGGCG AGCTTCGGCT TCAATCCCAC CGTTGATGGC GGCTGTTCTC AAAAGCTTGA AGTCCACCTG TTCGACTTTC AAGGCGATTT GTACGGACAA CGGTTGAACG TCCGCTTCCT GCACAAACTG CGCGACGAGG AAAAGTTTGA CGGTATGGAA GAACTGAAAA GGCGGATTGA AGCCGATATG GAAGCCGCAA AGTGTTGGTA G
Details for NegoA.01300.a.B1.GE40049
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK IRPGRHNAPD FPHGAAVTIG NFDGVHLGHK HILQKLRLEA DARGLPVVAV VFEPQPKEFF ALRTGKTPPC RISPLRTKLE LLEGTGCVDA AWVLRFDRNF SEISAQAFID RLLRQTLNTR YLLVGDDFRF GAGREGCFEL LAQQPDMQTE RTPSVIVEDI RTSSTAVRQA LSDGNLAYAK KLLGHDYVLG GRVVHGRKLG RTLNAPTANI RLPGHRYALG GVFVVEADGA FGTRRGVASF GFNPTVDGGC SQKLEVHLFD FQGDLYGQRL NVRFLHKLRD EEKFDGMEEL KRRIEXXXEA XXVLVSE
Validated NT Sequence
ttactcactt accaacactn nnnnggcttc cannnnnnnt tcaatccgcc ttttcagttc ttccataccg tcaaactttt cctcgtcgcg cagtttgtgc aggaagcgga cgttcaaccg ttgtccgtac aaatcgcctt gaaagtcgaa caaatggact tccagctttt gagaacagcc gccatcaacg gtgggattga agccgaagct cgccacgccg cgccgcgtgc cgaatgcgcc gtccgcctcg acgacgaaca cgccgccgag tgcataacgg tggccgggca gtcggatgtt ggcggtcggg gcgtttaagg tgcgcccgag ttttctgccg tgcaccaccc tgccccccaa aacgtagtcg tgccccaaaa gttttttcgc ataggcaagg ttgccgtctg aaagggcttg gcggacggcg gtactgctgg tgcggatgtc ttcgacaatg acagaaggcg tgcgctcggt ctgcatatcg ggctgttgtg ccaaaagttc aaaacagcct tcccgccccg ccccgaaacg gaaatcatcg ccgacgagca aatagcgcgt attcaaggtt tgacgcagca ggcggtcgat aaatgcttgc gcggatattt cggaaaaatt ccgatcgaaa cgcaaaaccc aggcggcatc gacgcaaccc gtcccttcca gcaattccag tttggtacgc agggggctga tacggcaggg cggggttttg ccggtacgga gtgcgaaaaa ttctttgggt tggggttcga aaacgacggc cacgacgggc aatccgcgtg cgtcggcttc gaggcggagt ttttggagga tgtgtttgtg tccgaggtgt acgccgtcga aattgcctat ggttacggcg gccccgtgcg gaaagtcggg cgcgttgtgc cgccccggcc tgattttcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMK IRPGRHNAPD FPHGAAVTIG NFDGVHLGHK HILQKLRLEA DARGLPVVAV IFEPQPKEFF AARTGKTPPC RISPLRTKLG LLEKTGCVDA AWVLRFDRNF SEISAQAFID RLLRQTLNTR YLLVGDDFRF GAGREGCFEL LAQQPDMQTE RTPSVIVEDI RTSSTTVRQA LSDGNLAYAK KLLGHDYVLG GRVVHGRKLG RTLNAPTANI RLPGHRYALG GVFVVEADGA FGTRRGVASF GFNPTVDGGC SQKLEVHLFD FQGDLYGQRL NVRFLHKLRD EEKFDGMEEL KRRIEADMEA AKCW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tcaggccggg gcggcacaac gcgcccgact ttccgcacgg ggccgccgta accataggca atttcgacgg cgtgcacctc ggacacaaac acatcctcca aaaactccgc ctcgaagccg acgcacgcgg attgcccgtc gttgccgtca ttttcgagcc gcaacccaaa gaattttttg ccgcccgcac aggcaaaacc ccgccgtgcc gtatcagccc cctgcgtacc aaactcgggc tgctcgaaaa aaccggttgc gtcgatgccg cctgggtttt gcgtttcgat cggaattttt ccgaaatatc cgcgcaagca tttatcgacc gcctgctgcg tcaaaccttg aatacgcgct atttgctcgt cggcgacgat ttccgtttcg gtgcggggcg ggaaggctgt tttgaacttt tggcacaaca gcccgatatg caaaccgagc gcacgccttc cgtcatcgtc gaagacatcc gcaccagcag caccaccgtc cgccaagccc tttcagacgg caaccttgcc tatgcgaaga aacttttggg gcacgactac gttttggggg gcagggtggt gcacggcaga aaactcgggc gcaccttaaa cgccccgacc gccaacatcc gactgcccgg ccaccgttat gccctcggcg gcgtgtttgt cgtcgaagcg gacggcgcat tcggcacgcg gcgcggcgtg gcgagcttcg gcttcaatcc caccgttgat ggcggctgtt ctcaaaagct tgaagtccac ctgttcgact ttcaaggcga tttgtacgga caacggttga acgtccgctt cctgcacaaa ctgcgcgacg aggaaaagtt tgacggtatg gaagaactga aaaggcggat tgaagccgat atggaagccg caaagtgttg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat