NegoA.01294.a
transketolase
| CENTER ID: | NegoA.01294.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.01294.a.B1.GE40048 | full length | 1 | 659 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.01294.a.B1.PS37968 | full length | 1 | 659 |
Structures
5VRB
DEPOSITED: 5/10/2017
DETERMINATION: XRay
CLONE: NegoA.01294.a.B1.GE40048
PROTEIN: NegoA.01294.a.B1.PS37968
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.822 |
| UniProt: | B4RKU9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQLANVIRF LSADAVQKAN SGHPGAPMGM AEMAETLWTK FLNHNPANPK FYNRDRFVLS NGHASMLLYS LLHLTGYNLS IEDLKNFRQL HSKTPGHPEY GYTDGVETTT GPLGQGIANA VGMALAEKIL AAEFNKDGLN IVDHYTYVFM GDGCLMEGVS HEACSLAGTL GLGKLIVLYD DNNISIDGKV DGWFTENIPQ RFESYGWHVV PNVNGHDTAA IQTAIEAARA ETGKPSIICC KTLIGKGSAN KEGSHKTHGA PLGADEIEAT RKHLGWAYPA FEIPQEIYDA WNAKEKGAKL EAGWNELFAQ YQAKYPAEAA EFVRRMDKKL PENFDEYVQT ALKEVCAKAE TVATRKASQN SIEILAKELP ELVGGSADLT PSNLTDWSNS VSVTRDKGGN YIHYGVREFG MGAIMNGLVL HGGVKPFGAT FLMFSEYERN ALRMAALMKI NPVFVFTHDS IGLGEDGPTH QPIEQTATLR LIPNMDVWRP CDTAESLVAW AEAAKAEDHP SCLIFSRQNL KFQARSEQQL NDIKRGAYVI SEAQGNAQAV IIATGSEVGL AVEAQKVLAG QGIAVRVVSM PSTSVFDRQD AAYQAAVLPE GLPRIAVEAG HTNGWYKYVG LNGAVVGINR FGESAPADLL FKAFGFTVDN VVDTVKSVL
NT Sequence
ATGTCTCAAC TGGCAAACGT AATCCGCTTC CTCTCGGCCG ATGCCGTTCA AAAAGCCAAT TCCGGCCACC CCGGCGCGCC TATGGGTATG GCGGAAATGG CGGAAACATT GTGGACGAAA TTCCTCAATC ACAATCCCGC CAATCCCAAA TTCTACAACC GCGACCGCTT CGTCCTCTCC AACGGTCATG CGTCTATGCT GTTGTACAGC CTGCTGCACC TGACCGGCTA CAACCTGAGC ATTGAAGATC TGAAAAACTT CCGCCAGCTG CACAGCAAAA CCCCCGGCCA TCCCGAATAC GGCTACACCG ACGGCGTGGA AACCACGACC GGCCCGTTGG GGCAAGGTAT TGCCAACGCA GTGGGCATGG CATTGGCAGA AAAAATCCTT GCCGCCGAAT TTAATAAAGA CGGTTTGAAC ATCGTCGATC ACTACACTTA CGTCTTTATG GGCGACGGCT GCCTGATGGA AGGCGTATCG CACGAAGCCT GCTCACTCGC CGGCACCTTG GGCTTGGGCA AACTGATTGT TTTGTATGAC GATAACAATA TCTCCATCGA CGGCAAAGTG GACGGCTGGT TTACCGAAAA CATCCCGCAA CGCTTTGAAA GCTACGGCTG GCACGTCGTT CCCAACGTAA ACGGCCACGA CACCGCCGCC ATTCAGACGG CCATCGAAGC CGCCCGCGCC GAAACCGGCA AGCCGTCCAT CATCTGCTGC AAAACCTTAA TCGGCAAAGG CAGCGCCAAC AAAGAAGGCA GCCACAAAAC CCACGGCGCG CCTTTGGGTG CGGACGAAAT CGAAGCCACG CGCAAACATT TGGGTTGGGC TTATCCTGCG TTTGAAATCC CGCAAGAAAT TTACGATGCG TGGAATGCCA AAGAAAAAGG CGCGAAACTG GAAGCCGGAT GGAACGAACT GTTCGCGCAA TATCAAGCCA AATATCCTGC CGAAGCCGCA GAATTCGTGC GCCGCATGGA TAAAAAGCTG CCGGAAAACT TTGACGAGTA CGTTCAGACG GCATTGAAAG AAGTGTGCGC CAAAGCGGAA ACCGTCGCCA CCCGCAAAGC CAGCCAAAAC AGCATCGAAA TCTTGGCAAA AGAGCTGCCC GAACTGGTGG GCGGTTCTGC CGACCTGACC CCGTCCAACC TGACCGACTG GTCAAACAGC GTCTCCGTTA CCCGCGACAA AGGCGGCAAC TACATCCACT ACGGCGTGCG CGAGTTCGGT ATGGGTGCGA TTATGAACGG TTTGGTATTG CACGGCGGTG TAAAACCCTT CGGCGCGACT TTCCTGATGT TCAGCGAATA CGAACGCAAC GCCTTACGCA TGGCGGCACT GATGAAAATC AACCCCGTCT TCGTATTCAC CCACGATTCC ATCGGTTTGG GCGAGGACGG CCCAACCCAC CAGCCTATCG AGCAAACCGC CACCCTGCGC CTGATTCCGA ATATGGACGT ATGGCGGCCG TGCGACACCG CAGAATCTCT GGTCGCTTGG GCAGAAGCGG CAAAAGCCGA AGACCACCCG TCCTGCCTGA TTTTCAGCCG TCAAAACCTG AAATTCCAAG CACGCAGCGA GCAACAACTG AACGACATCA AACGCGGTGC TTACGTAATC AGTGAAGCCC AAGGCAACGC CCAAGCCGTC ATCATTGCCA CCGGTTCCGA GGTAGGATTG GCTGTAGAAG CGCAAAAAGT ATTGGCAGGA CAAGGTATTG CCGTGCGCGT CGTTTCCATG CCGTCCACCA GCGTCTTCGA CCGCCAAGAC GCCGCCTATC AAGCCGCCGT CCTACCGGAA GGCCTGCCGC GCATCGCCGT AGAAGCCGGA CACACCAACG GCTGGTACAA ATATGTCGGA CTGAACGGCG CAGTCGTCGG CATCAACCGC TTCGGCGAAT CCGCCCCTGC CGATCTGCTG TTCAAAGCAT TCGGCTTTAC CGTGGACAAT GTGGTTGATA CGGTGAAATC CGTGCTGTAA
Details for NegoA.01294.a.B1.GE40048
| HARVESTED ON: | 3/24/2016 |
| SEQUENCED ON: | 3/25/2016 |
| EXPECTED MW: | 73kDa |
| OBSERVED MW: | 73kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS QLANVIRFLS ADAVQKANSG HPGAPMGMAE MAETLWTKFL NHNPANPKFY NRDRFVLSNG HASMLLYSLL HLTGYNLSIE DLKNFRQLHS KTPGHPEYGY TDGVETTTGP LGQGIANAVG MALAEKILAA EFNKDGLNIV DHYTYVFMGD GCLMEGVSHE ACSLAGTLGL GKLIVLYDDN NISIDGKVDG WFTENIPQRF ESYGWHVVPN VNGHDTAAIQ TAIEAARAET GKPSIICCKT LIGKGSANKE GSHKTHGAPL GADEIEATRK HLGWAYPAFE IPQEIYDAWN AKEKGAKLEA GWNELFAQYQ AKYPAEAAEF VRRMDKKLPE NFDEYVQTAL KEVCAKAETV ATRKASQNSI EILAKELPEL VGGSADLTPS NLTDWSNSVS VTRDKGGNYI HYGVREFGMG AIMNGLVLHG GVKPFGATFL MFSEYERNAL RMAALMKINP VFVFTHDSIG LGEDGPTHQP IEQTATLRLI PNMDVWRPCD TAESLVAWAE AAKAEDHPSC LIFSRQNLKF QARSEQQLND IKRGAYVISE AQGNAQAVII ATGSEVGLAV EAQKVLAGQG IAVRVVSMPS TSVFDRQDAA YQAAVLPEGL PRIAVEAGHT NGWYKYVGLN GAVVGINRFG ESAPADLLFK AFGFTVDNVV DTVKSVL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc aactggcaaa cgtaatccgc ttcctctcgg ccgatgccgt tcaaaaagcc aattccggcc accccggcgc gcctatgggt atggcggaaa tggcggaaac attgtggacg aaattcctca atcacaatcc cgccaatccc aaattctaca accgcgaccg cttcgtcctc tccaacggtc atgcgtctat gctgttgtac agcctgctgc acctgaccgg ctacaacctg agcattgaag atctgaaaaa cttccgccag ctgcacagca aaacccccgg ccatcccgaa tacggctaca ccgacggcgt ggaaaccacg accggcccgt tggggcaagg tattgccaac gcagtgggca tggcattggc agaaaaaatc cttgccgccg aatttaataa agacggtttg aacatcgtcg atcactacac ttacgtcttt atgggcgacg gctgcctgat ggaaggcgta tcgcacgaag cctgctcact cgccggcacc ttgggcttgg gcaaactgat tgttttgtat gacgataaca atatctccat cgacggcaaa gtggacggct ggtttaccga aaacatcccg caacgctttg aaagctacgg ctggcacgtc gttcccaacg taaacggcca cgacaccgcc gccattcaga cggccatcga agccgcccgc gccgaaaccg gcaagccgtc catcatctgc tgcaaaacct taatcggcaa aggcagcgcc aacaaagaag gcagccacaa aacccacggc gcgcctttgg gtgcggacga aatcgaagcc acgcgcaaac atttgggttg ggcttatcct gcgtttgaaa tcccgcaaga aatttacgat gcgtggaatg ccaaagaaaa aggcgcgaaa ctggaagccg gatggaacga actgttcgcg caatatcaag ccaaatatcc tgccgaagcc gcagaattcg tgcgccgcat ggataaaaag ctgccggaaa actttgacga gtacgttcag acggcattga aagaagtgtg cgccaaagcg gaaaccgtcg ccacccgcaa agccagccaa aacagcatcg aaatcttggc aaaagagctg cccgaactgg tgggcggttc tgccgacctg accccgtcca acctgaccga ctggtcaaac agcgtctccg ttacccgcga caaaggcggc aactacatcc actacggcgt gcgcgagttc ggtatgggtg cgattatgaa cggtttggta ttgcacggcg gtgtaaaacc cttcggcgcg actttcctga tgttcagcga atacgaacgc aacgccttac gcatggcggc actgatgaaa atcaaccccg tcttcgtatt cacccacgat tccatcggtt tgggcgagga cggcccaacc caccagccta tcgagcaaac cgccaccctg cgcctgattc cgaatatgga cgtatggcgg ccgtgcgaca ccgcagaatc tctggtcgct tgggcagaag cggcaaaagc cgaagaccac ccgtcctgcc tgattttcag ccgtcaaaac ctgaaattcc aagcacgcag cgagcaacaa ctgaacgaca tcaaacgcgg tgcttacgta atcagtgaag cccaaggcaa cgcccaagcc gtcatcattg ccaccggttc cgaggtagga ttggctgtag aagcgcaaaa agtattggca ggacaaggta ttgccgtgcg cgtcgtttcc atgccgtcca ccagcgtctt cgaccgccaa gacgccgcct atcaagccgc cgtcctaccg gaaggcctgc cgcgcatcgc cgtagaagcc ggacacacca acggctggta caaatatgtc ggactgaacg gcgcagtcgt cggcatcaac cgcttcggcg aatccgcccc tgccgatctg ctgttcaaag cattcggctt taccgtggac aatgtggttg atacggtgaa atccgtgctg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for NegoA.01294.a.B1.PS37968
| PURIFICATION DATe: | 7/7/2016 |
| CONCENTRATION: | 22.9mg/ml |
| OBSERVED MW: | 75kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS QLANVIRFLS ADAVQKANSG HPGAPMGMAE MAETLWTKFL NHNPANPKFY NRDRFVLSNG HASMLLYSLL HLTGYNLSIE DLKNFRQLHS KTPGHPEYGY TDGVETTTGP LGQGIANAVG MALAEKILAA EFNKDGLNIV DHYTYVFMGD GCLMEGVSHE ACSLAGTLGL GKLIVLYDDN NISIDGKVDG WFTENIPQRF ESYGWHVVPN VNGHDTAAIQ TAIEAARAET GKPSIICCKT LIGKGSANKE GSHKTHGAPL GADEIEATRK HLGWAYPAFE IPQEIYDAWN AKEKGAKLEA GWNELFAQYQ AKYPAEAAEF VRRMDKKLPE NFDEYVQTAL KEVCAKAETV ATRKASQNSI EILAKELPEL VGGSADLTPS NLTDWSNSVS VTRDKGGNYI HYGVREFGMG AIMNGLVLHG GVKPFGATFL MFSEYERNAL RMAALMKINP VFVFTHDSIG LGEDGPTHQP IEQTATLRLI PNMDVWRPCD TAESLVAWAE AAKAEDHPSC LIFSRQNLKF QARSEQQLND IKRGAYVISE AQGNAQAVII ATGSEVGLAV EAQKVLAGQG IAVRVVSMPS TSVFDRQDAA YQAAVLPEGL PRIAVEAGHT NGWYKYVGLN GAVVGINRFG ESAPADLLFK AFGFTVDNVV DTVKSVL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTC AACTGGCAAA CGTAATCCGC TTCCTCTCGG CCGATGCCGT TCAAAAAGCC AATTCCGGCC ACCCCGGCGC GCCTATGGGT ATGGCGGAAA TGGCGGAAAC ATTGTGGACG AAATTCCTCA ATCACAATCC CGCCAATCCC AAATTCTACA ACCGCGACCG CTTCGTCCTC TCCAACGGTC ATGCGTCTAT GCTGTTGTAC AGCCTGCTGC ACCTGACCGG CTACAACCTG AGCATTGAAG ATCTGAAAAA CTTCCGCCAG CTGCACAGCA AAACCCCCGG CCATCCCGAA TACGGCTACA CCGACGGCGT GGAAACCACG ACCGGCCCGT TGGGGCAAGG TATTGCCAAC GCAGTGGGCA TGGCATTGGC AGAAAAAATC CTTGCCGCCG AATTTAATAA AGACGGTTTG AACATCGTCG ATCACTACAC TTACGTCTTT ATGGGCGACG GCTGCCTGAT GGAAGGCGTA TCGCACGAAG CCTGCTCACT CGCCGGCACC TTGGGCTTGG GCAAACTGAT TGTTTTGTAT GACGATAACA ATATCTCCAT CGACGGCAAA GTGGACGGCT GGTTTACCGA AAACATCCCG CAACGCTTTG AAAGCTACGG CTGGCACGTC GTTCCCAACG TAAACGGCCA CGACACCGCC GCCATTCAGA CGGCCATCGA AGCCGCCCGC GCCGAAACCG GCAAGCCGTC CATCATCTGC TGCAAAACCT TAATCGGCAA AGGCAGCGCC AACAAAGAAG GCAGCCACAA AACCCACGGC GCGCCTTTGG GTGCGGACGA AATCGAAGCC ACGCGCAAAC ATTTGGGTTG GGCTTATCCT GCGTTTGAAA TCCCGCAAGA AATTTACGAT GCGTGGAATG CCAAAGAAAA AGGCGCGAAA CTGGAAGCCG GATGGAACGA ACTGTTCGCG CAATATCAAG CCAAATATCC TGCCGAAGCC GCAGAATTCG TGCGCCGCAT GGATAAAAAG CTGCCGGAAA ACTTTGACGA GTACGTTCAG ACGGCATTGA AAGAAGTGTG CGCCAAAGCG GAAACCGTCG CCACCCGCAA AGCCAGCCAA AACAGCATCG AAATCTTGGC AAAAGAGCTG CCCGAACTGG TGGGCGGTTC TGCCGACCTG ACCCCGTCCA ACCTGACCGA CTGGTCAAAC AGCGTCTCCG TTACCCGCGA CAAAGGCGGC AACTACATCC ACTACGGCGT GCGCGAGTTC GGTATGGGTG CGATTATGAA CGGTTTGGTA TTGCACGGCG GTGTAAAACC CTTCGGCGCG ACTTTCCTGA TGTTCAGCGA ATACGAACGC AACGCCTTAC GCATGGCGGC ACTGATGAAA ATCAACCCCG TCTTCGTATT CACCCACGAT TCCATCGGTT TGGGCGAGGA CGGCCCAACC CACCAGCCTA TCGAGCAAAC CGCCACCCTG CGCCTGATTC CGAATATGGA CGTATGGCGG CCGTGCGACA CCGCAGAATC TCTGGTCGCT TGGGCAGAAG CGGCAAAAGC CGAAGACCAC CCGTCCTGCC TGATTTTCAG CCGTCAAAAC CTGAAATTCC AAGCACGCAG CGAGCAACAA CTGAACGACA TCAAACGCGG TGCTTACGTA ATCAGTGAAG CCCAAGGCAA CGCCCAAGCC GTCATCATTG CCACCGGTTC CGAGGTAGGA TTGGCTGTAG AAGCGCAAAA AGTATTGGCA GGACAAGGTA TTGCCGTGCG CGTCGTTTCC ATGCCGTCCA CCAGCGTCTT CGACCGCCAA GACGCCGCCT ATCAAGCCGC CGTCCTACCG GAAGGCCTGC CGCGCATCGC CGTAGAAGCC GGACACACCA ACGGCTGGTA CAAATATGTC GGACTGAACG GCGCAGTCGT CGGCATCAAC CGCTTCGGCG AATCCGCCCC TGCCGATCTG CTGTTCAAAG CATTCGGCTT TACCGTGGAC AATGTGGTTG ATACGGTGAA ATCCGTGCTG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT