NegoA.01161.a

pyruvate kinase

CENTER ID: NegoA.01161.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
NegoA.01161.a.B1.GE40045 full length 1 490
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
Molecular models
METHOD RESULTS
comparative modelling Robetta_85042
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|521006.8.peg.2556
UniProt: B4RPV5
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNQTSRDLTR ISHNTKIVAT LGPGSNNVEL LEDMIRVGGL NVVRFNFSHG TPEFHQENAR IVREAAKRAG QEIAIIADLQ GPKIRVGKIA GGGIELNKGE TLVLDAALEG EGTREAVGLD YRDLPDDVAA GDVLWLDDGL LTLTVESVEG SRIITRVENS HILKSNKGIN KRGGGLSAGA LTEKDFRDLK TAIAIGCDYL AISFVKSAED LHIARAKVEE EMKGSTAVRP GLVSKIERVE AIENLDEIIL AGDGIMVARG DLAVEVGHAA VPALQKRMIR RARELRRFSI TATQMMESMI TNPVPTRAEV SDVANAVLDG TDAVMCSAET AVGAYPFETV SQMAIICAAA EKEQDSLNGV AEQTEYPEAV STNLAIAGGA VSVARAVHAK AIVALTESGS TAFEISRHNI TLPIFALTPS VSAQRRMAMY RGVRPLILAT STDHDTALNE VETMLVEHNI LHSGDQYIIT SGSQMRESGS TNTLEVLRVK
NT Sequence
ATGAACCAGA CAAGCCGCGA TCTGACCCGC ATCAGCCACA ACACCAAAAT CGTCGCCACC CTTGGGCCGG GCAGCAACAA CGTCGAACTG TTGGAAGACA TGATCCGCGT CGGCGGCCTG AACGTCGTCC GCTTCAACTT CAGCCACGGC ACGCCCGAAT TCCATCAGGA AAACGCCCGC ATCGTGCGCG AGGCGGCAAA ACGCGCCGGA CAGGAAATCG CCATCATTGC CGATCTGCAG GGCCCGAAAA TCCGCGTGGG CAAAATCGCC GGCGGCGGCA TCGAATTGAA CAAAGGCGAA ACATTGGTAC TCGATGCCGC GCTCGAAGGC GAAGGCACGC GCGAGGCGGT CGGTTTGGAC TACCGCGACC TGCCCGACGA CGTTGCCGCA GGCGATGTCT TGTGGCTGGA CGACGGCCTG CTGACCCTGA CCGTGGAATC CGTCGAAGGC AGCAGGATTA TCACAAGGGT GGAAAACAGC CACATCCTGA AAAGCAACAA AGGCATCAAC AAACGCGGCG GCGGTTTGTC CGCAGGCGCG TTGACCGAAA AAGACTTCCG CGACCTGAAA ACCGCGATTG CCATCGGTTG CGACTATCTC GCCATCAGCT TTGTGAAATC CGCCGAAGAC CTGCACATCG CGCGCGCCAA AGTCGAAGAG GAAATGAAGG GCAGCACTGC CGTCCGCCCC GGTTTGGTTT CCAAAATCGA ACGCGTAGAA GCGATTGAAA ACCTTGATGA AATCATCCTT GCCGGCGACG GCATTATGGT TGCGCGCGGC GACTTGGCGG TCGAAGTCGG ACACGCCGCC GTCCCCGCCC TGCAAAAACG GATGATCCGC CGCGCCCGCG AGTTGCGCCG CTTCAGCATT ACGGCGACGC AAATGATGGA ATCGATGATT ACCAACCCCG TGCCGACCCG CGCGGAAGTC AGCGATGTGG CAAACGCGGT ATTGGACGGC ACCGATGCGG TGATGTGTTC CGCCGAAACC GCCGTCGGCG CGTATCCGTT TGAAACCGTC AGCCAAATGG CGATTATCTG CGCGGCTGCG GAAAAAGAAC AGGATTCGCT CAACGGCGTT GCCGAACAGA CAGAGTATCC CGAAGCCGTC AGCACCAACT TAGCGATTGC CGGCGGCGCG GTCAGCGTGG CGCGCGCGGT TCACGCCAAA GCCATCGTCG CCCTGACCGA AAGCGGTTCG ACCGCCTTTG AAATCAGCCG CCACAATATT ACCCTGCCGA TTTTCGCGCT GACCCCGAGC GTTTCCGCCC AACGCCGTAT GGCGATGTAC CGGGGCGTGC GCCCGCTGAT TTTGGCAACC AGCACCGACC ACGACACGGC GTTGAACGAA GTCGAAACGA TGCTGGTGGA ACACAACATC CTGCATTCCG GCGACCAGTA CATCATCACC AGCGGCTCGC AAATGCGCGA ATCCGGTTCG ACCAATACGC TGGAAGTGCT GCGCGTCAAA TAA
Details for NegoA.01161.a.B1.GE40045
HARVESTED ON: data unavailable
SEQUENCED ON: 3/25/2016
EXPECTED MW: 54kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMN QTSRDLTRIS HNTKIVATLG PGSNNVELLE DMIRVGGLNV VRFNFSHGTP EFHQENARIV REAAKRAGQE IAIIADLQGP KIRVGKIAGG GIELNKGETL VLDAALEGEG TREAVGLDYR DLPDDVAAGD VLWLDDGLLT LTVESVEGSR IITRVENSHI LKSNKGINKR GGGLSAGALT EKDFRDLKTA IAIGCDYLAI SFVKSAEDLH IARAKVEEEM KGSTAVRPGL VSKIERVEAI ENLDEIILAG DGIMVARGDL AVEVGHAAVP ALQKRMIRRA RELRRFSITA TQMMESMITN PVPTRAEVSD VANAVLDGTD AVMCSAETAV GAYPFETVSQ MAIICAAAEK EQDSLNGVAE QTEYPEAVST NLAIAGGAVS VARAVHAKAI VALTESGSTA FEISRHNITL PIFALTPSVS AQRRMAMYRG VRPLILATST DHDTALNEVE TMLVEHNILH SGDQYIITSG SQMRESGSTN
Validated NT Sequence
gtattggtcg aaccggattc gcgcatttgc gagccgctgg tgatgatgta ctggtcgccg gaatgcagga tgttgtgttc caccagcatc gtttcgactt cgttcaacgc cgtgtcgtgg tcggtgctgg ttgccaaaat cagcgggcgc acgccccggt acatcgccat acggcgttgg gcggaaacgc tcggggtcag cgcgaaaatc ggtagggtaa tattgtggcg gctgatttca aaggcggtcg aaccgctttc ggtcagggcg acgatggctt tggcgtgaac cgcgcgcgcc acgctgaccg cgccgccggc aatcgctaag ttggtgctga cggcttcggg atactccgtc tgttcggcaa cgccgttgag cgaatcctgc tctttttccg cagccgcgca gataatcgcc atttggctga cggtttcaaa cggatacgcg ccgacggcgg tttcggcgga acacatcacc gcatcggtgc cgtccaatac cgcgtttgcc acatcgctga cttccgcgcg ggtcggcacg gggttggtaa tcatcgattc catcatttgc gtcgccgtaa tgctgaagcg gcgcaactcg cgggcgcggc ggatcatccg tttttgcagg gcggggacgg cggcgtgtcc gacttcgacc gccaagtcgc cgcgcgcaac cataatgccg tcgccggcaa ggatgatttc atcaaggttt tcaatcgctt ctacgcgttc gattttggaa accaaaccgg ggcggacggc agtgctgccc ttcatttcct cttcgacttt ggcgcgcgcg atgtgcaggt cttcggcgga tttcacaaag ctgatggcga ggtagtcgca accgatggca atcgcggttt tcaggtcgcg gaagtctttt tcggtcaacg cgcctgcgga caaaccgccg ccgcgtttgt tgatgccttt gttgcttttc aggatgtggc tgttttccac ccttgtgata atcctgctgc cttcgacgga ttccacggtc agggtcagca ggccgtcgtc cagccacaag acatcgcctg cggcaacgtc gtcgggcagg tcgcggtagt ccaaaccgac cgcctcgcgc gtgccttcgc cttcgagcgc ggcatcgagt accaatgttt cgcctttgtt caattcgatg ccgccgccgg cgattttgcc cacgcggatt ttcgggccct gcagatcggc aatgatggcg atttcctgtc cggcgcgttt tgccgcctcg cgcacgatgc gggcgttttc ctgatggaat tcgggcgtgc cgtggctgaa gttgaagcgg acgacgttca ggccgccgac gcggatcatg tcttccaaca gttcgacgtt gttgctgccc ggcccaaggg tggcgacgat tttggtgttg tggctgatgc gggtcagatc gcggcttgtc tggttcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMN QTSRDLTRIS HNTKIVATLG PGSNNVELLE DMIRVGGLNV VRFNFSHGTP EFHQENARIV REAAKRAGQE IAIIADLQGP KIRVGKIAGG GIELNKGETL VLDAALEGEG TREAVGLDYR DLPDDVAAGD VLWLDDGLLT LTVESVEGSR IITRVENSHI LKSNKGINKR GGGLSAGALT EKDFRDLKTA IAIGCDYLAI SFVKSAEDLH IARAKVEEEM KGSTAVRPGL VSKIERVEAI ENLDEIILAG DGIMVARGDL AVEVGHAAVP ALQKRMIRRA RELRRFSITA TQMMESMITN PVPTRAEVSD VANAVLDGTD AVMCSAETAV GAYPFETVSQ MAIICAAAEK EQDSLNGVAE QTEYPEAVST NLAIAGGAVS VARAVHAKAI VALTESGSTA FEISRHNITL PIFALTPSVS AQRRMAMYRG VRPLILATST DHDTALNEVE TMLVEHNILH SGDQYIITSG SQMRESGSTN TLEVLRVK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc agacaagccg cgatctgacc cgcatcagcc acaacaccaa aatcgtcgcc acccttgggc cgggcagcaa caacgtcgaa ctgttggaag acatgatccg cgtcggcggc ctgaacgtcg tccgcttcaa cttcagccac ggcacgcccg aattccatca ggaaaacgcc cgcatcgtgc gcgaggcggc aaaacgcgcc ggacaggaaa tcgccatcat tgccgatctg cagggcccga aaatccgcgt gggcaaaatc gccggcggcg gcatcgaatt gaacaaaggc gaaacattgg tactcgatgc cgcgctcgaa ggcgaaggca cgcgcgaggc ggtcggtttg gactaccgcg acctgcccga cgacgttgcc gcaggcgatg tcttgtggct ggacgacggc ctgctgaccc tgaccgtgga atccgtcgaa ggcagcagga ttatcacaag ggtggaaaac agccacatcc tgaaaagcaa caaaggcatc aacaaacgcg gcggcggttt gtccgcaggc gcgttgaccg aaaaagactt ccgcgacctg aaaaccgcga ttgccatcgg ttgcgactat ctcgccatca gctttgtgaa atccgccgaa gacctgcaca tcgcgcgcgc caaagtcgaa gaggaaatga agggcagcac tgccgtccgc cccggtttgg tttccaaaat cgaacgcgta gaagcgattg aaaaccttga tgaaatcatc cttgccggcg acggcattat ggttgcgcgc ggcgacttgg cggtcgaagt cggacacgcc gccgtccccg ccctgcaaaa acggatgatc cgccgcgccc gcgagttgcg ccgcttcagc attacggcga cgcaaatgat ggaatcgatg attaccaacc ccgtgccgac ccgcgcggaa gtcagcgatg tggcaaacgc ggtattggac ggcaccgatg cggtgatgtg ttccgccgaa accgccgtcg gcgcgtatcc gtttgaaacc gtcagccaaa tggcgattat ctgcgcggct gcggaaaaag aacaggattc gctcaacggc gttgccgaac agacagagta tcccgaagcc gtcagcacca acttagcgat tgccggcggc gcggtcagcg tggcgcgcgc ggttcacgcc aaagccatcg tcgccctgac cgaaagcggt tcgaccgcct ttgaaatcag ccgccacaat attaccctgc cgattttcgc gctgaccccg agcgtttccg cccaacgccg tatggcgatg taccggggcg tgcgcccgct gattttggca accagcaccg accacgacac ggcgttgaac gaagtcgaaa cgatgctggt ggaacacaac atcctgcatt ccggcgacca gtacatcatc accagcggct cgcaaatgcg cgaatccggt tcgaccaata cgctggaagt gctgcgcgtc aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat