NegoA.01136.a

1-deoxy-D-xylulose 5-phosphate reductoisomerase

CENTER ID: NegoA.01136.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.01136.a.B1.GE40044 full length 1 411
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.2675
UniProt: B4RR17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSENASDGI RYKVILIMTP QVLTILGSTG SIGESTLDVV SRHPEKFRVF ALAGHKQVEK LAAQCQTFRP EYAVVADAEH AARLEALLKR DGTATQVLHG AQALVDVASA DEVSGVMCAI VGAAGLPSAL AAAQKGKTIY LANKETLVVS GALFMETARA NGAAVLPVDS EHNAIFQVLP RDYTDRLNEH GIDSIILTAS GGPFLTTDLS TFDSITPEQA VKHPNWRMGR KISVDSATMA NKGLELIEAH WLFNCPPDKL EVVIHPQSVI HSMVRYRDGS VLAQLGNPDM RTPIAYCLGL PERIDSGVGK LDFGALSALT FQKPDFGRFP CLKFAYETMN AGGAAPCVLN AANETAVAAF LDGQIKFTDI AKTVAHCLAQ DFSNGMGDIE GLLAQDARTR AQARAFIGTL R
NT Sequence
ATGCCGTCTG AAAACGCTTC AGACGGCATC CGGTATAAAG TTATCCTCAT TATGACACCA CAAGTCCTGA CCATATTAGG CAGTACCGGC AGCATAGGCG AAAGCACGCT GGACGTTGTC TCCCGCCATC CCGAAAAATT CCGCGTATTC GCGCTGGCAG GGCATAAGCA GGTCGAGAAA CTGGCGGCTC AATGTCAAAC GTTCCGCCCC GAATATGCCG TCGTTGCCGA TGCCGAACAC GCCGCCCGGC TTGAAGCCCT GTTGAAACGC GACGGCACGG CGACTCAGGT TTTACACGGC GCGCAGGCAT TGGTTGACGT TGCGTCTGCC GACGAAGTCA GCGGCGTCAT GTGCGCCATC GTCGGGGCGG CGGGGCTGCC TTCCGCGCTC GCAGCGGCGC AAAAAGGCAA AACCATTTAT CTGGCAAACA AAGAAACGCT GGTGGTTTCC GGCGCGTTGT TTATGGAAAC CGCCCGCGCA AACGGCGCGG CAGTGTTGCC CGTCGACAGC GAACACAACG CCATTTTCCA AGTTTTGCCG CGCGATTACA CAGACCGTCT GAACGAACAC GGCATCGATT CGATTATCCT GACCGCTTCC GGCGGTCCGT TTTTAACAAC CGATTTAAGC ACGTTCGACA GCATTACGCC CGAGCAGGCG GTCAAACACC CCAATTGGCG TATGGGGCGC AAAATCTCCG TCGATTCAGC CACTATGGCA AACAAGGGCT TGGAACTGAT TGAAGCGCAT TGGCTGTTCA ACTGTCCGCC CGACAAACTC GAAGTCGTCA TCCATCCCCA ATCCGTGATA CACAGTATGG TGCGCTACCG CGACGGCTCC GTGCTGGCGC AACTGGGCAA TCCCGATATG CGAACGCCCA TCGCCTATTG TTTGGGCTTG CCCGAGCGCA TCGATTCGGG TGTCGGCAAA CTCGATTTCG GCGCATTGTC CGCGCTGACC TTCCAAAAGC CCGACTTCGG CCGCTTCCCC TGCCTGAAGT TCGCCTATGA AACCATGAAC GCAGGCGGAG CCGCGCCCTG CGTATTGAAC GCCGCCAACG AAACCGCCGT CGCCGCCTTT TTGGACGGAC AGATTAAGTT TACCGACATT GCCAAAACCG TCGCCCACTG TCTTGCACAA GACTTTTCAA ACGGCATGGG CGATATAGAA GGACTGTTGG CGCAAGATGC CCGGACACGC GCACAAGCGC GGGCATTTAT CGGCACACTG CGCTGA
Details for NegoA.01136.a.B1.GE40044
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMP SENASDGIRY KVILIMTPQV LTILGSTGSI GESTLDVVSR HPEKFRVFAL AGHKQVEKLA AQCQTFRPEY AVVADAEHAA RLEALLKRDG TATQVLHGAQ ALVDVASADE VSGVMCAIVG AAGLPSALAA AQKGKTIYLA NKETLVVSGA LFMETARANG AAVLPVDSEH NAIFQVLPRD YTDRLNEHGI DSIILTASGG PFLTTDLSTF DSITPEQAVK HPNWRMGRKI SVDSATMANK GLELIEAHWL FNCPPDKLEV VIHPQSVIHS MVRYRDGSVL AQLGNPDMRT PIAYCLGLPE RIDSGVGKLD FGALSALTFQ KPDFGRFPCL KFAYETINAG GAAPCVLNAA NETAVAAFLD GQIKFTDIAK TVAHCLAQDF SNGMGDIEGL LAQDARTRAQ A
Validated NT Sequence
cgcgcttgtg cgcgtgtccg ggcatcttgc gccaacagtc cttctatatc gcccatgccg tttgaaaagt cttgtgcaag acagtgggcg acggttttgg caatgtcggt aaacttaatc tgtccgtcca aaaaggcggc gacggcggtt tcgttggcgg cgttcaatac gcagggcgcg gctccgcctg cgtttatggt ttcataggcg aacttcaggc aggggaagcg gccgaagtcg ggcttttgga aggtcagcgc ggacaatgcg ccgaaatcga gtttgccgac acccgaatcg atgcgctcgg gcaagcccaa acaataggcg atgggcgttc gcatatcggg attgcccagt tgcgccagca cggagccgtc gcggtagcgc accatactgt gtatcacgga ttggggatgg atgacgactt cgagtttgtc gggcggacag ttgaacagcc aatgcgcttc aatcagttcc aagcccttgt ttgccatagt ggctgaatcg acggagattt tgcgccccat acgccaattg gggtgtttga ccgcctgctc gggcgtaatg ctgtcgaacg tgcttaaatc ggttgttaaa aacgggccgc cggaagcggt caggataatc gaatcgatgc cgtgttcgtt cagacggtct gtgtaatcgc gcggcaaaac ttggaaaatg gcgttgtgtt cgctgtcgac gggcaacact gccgcgccgt ttgcgcgggc ggtttccata aacaacgcgc cggaaaccac cagcgtttct ttgtttgcca gataaatggt tttgcctttt tgcgccgctg cgagcgcgga aggcagcccc gccgccccga cgatggcgca catgacgccg ctgacttcgt cggcagacgc aacgtcaacc aatgcctgcg cgccgtgtaa aacctgagtc gccgtgccgt cgcgtttcaa cagggcttca agccgggcgg cgtgttcggc atcggcaacg acggcatatt cggggcggaa cgtttgacat tgagccgcca gtttctcgac ctgcttatgc cctgccagcg cgaatacgcg gaatttttcg ggatggcggg agacaacgtc cagcgtgctt tcgcctatgc tgccggtact gcctaatatg gtcaggactt gtggtgtcat aatgaggata actttatacc ggatgccgtc tgaagcgttt tcagacggca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMP SENASDGIRY KVILIMTPQV LTILGSTGSI GESTLDVVSR HPEKFRVFAL AGHKQVEKLA AQCQTFRPEY AVVADAEHAA RLEALLKRDG TATQVLHGAQ ALVDVASADE VSGVMCAIVG AAGLPSALAA AQKGKTIYLA NKETLVVSGA LFMETARANG AAVLPVDSEH NAIFQVLPRD YTDRLNEHGI DSIILTASGG PFLTTDLSTF DSITPEQAVK HPNWRMGRKI SVDSATMANK GLELIEAHWL FNCPPDKLEV VIHPQSVIHS MVRYRDGSVL AQLGNPDMRT PIAYCLGLPE RIDSGVGKLD FGALSALTFQ KPDFGRFPCL KFAYETMNAG GAAPCVLNAA NETAVAAFLD GQIKFTDIAK TVAHCLAQDF SNGMGDIEGL LAQDARTRAQ ARAFIGTLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgt ctgaaaacgc ttcagacggc atccggtata aagttatcct cattatgaca ccacaagtcc tgaccatatt aggcagtacc ggcagcatag gcgaaagcac gctggacgtt gtctcccgcc atcccgaaaa attccgcgta ttcgcgctgg cagggcataa gcaggtcgag aaactggcgg ctcaatgtca aacgttccgc cccgaatatg ccgtcgttgc cgatgccgaa cacgccgccc ggcttgaagc cctgttgaaa cgcgacggca cggcgactca ggttttacac ggcgcgcagg cattggttga cgttgcgtct gccgacgaag tcagcggcgt catgtgcgcc atcgtcgggg cggcggggct gccttccgcg ctcgcagcgg cgcaaaaagg caaaaccatt tatctggcaa acaaagaaac gctggtggtt tccggcgcgt tgtttatgga aaccgcccgc gcaaacggcg cggcagtgtt gcccgtcgac agcgaacaca acgccatttt ccaagttttg ccgcgcgatt acacagaccg tctgaacgaa cacggcatcg attcgattat cctgaccgct tccggcggtc cgtttttaac aaccgattta agcacgttcg acagcattac gcccgagcag gcggtcaaac accccaattg gcgtatgggg cgcaaaatct ccgtcgattc agccactatg gcaaacaagg gcttggaact gattgaagcg cattggctgt tcaactgtcc gcccgacaaa ctcgaagtcg tcatccatcc ccaatccgtg atacacagta tggtgcgcta ccgcgacggc tccgtgctgg cgcaactggg caatcccgat atgcgaacgc ccatcgccta ttgtttgggc ttgcccgagc gcatcgattc gggtgtcggc aaactcgatt tcggcgcatt gtccgcgctg accttccaaa agcccgactt cggccgcttc ccctgcctga agttcgccta tgaaaccatg aacgcaggcg gagccgcgcc ctgcgtattg aacgccgcca acgaaaccgc cgtcgccgcc tttttggacg gacagattaa gtttaccgac attgccaaaa ccgtcgccca ctgtcttgca caagactttt caaacggcat gggcgatata gaaggactgt tggcgcaaga tgcccggaca cgcgcacaag cgcgggcatt tatcggcaca ctgcgctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t