SSGCID: Target Page

NegoA.01068.a

phosphoribosylaminoimidazole-succinocarboxamide synthase

CENTER ID:	NegoA.01068.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.01068.a.B1.GE40043	full length		1	287

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.01068.a.B1.PS37960	full length		1	287

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.531
UniProt:	B4RK27

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSEIGLVKIY SGKVRDLYEI DDKRMLMVAS DRLSAFDVIL DAPIPGKGEI LTQISNFWFK KLAHIMPNHF TGDTVYDVLP ENEAKALEKR AVVAKKLTPV KVEAIVRGYL AGSGWKDYQK TGSVCGIRLP EGMREAQQLP EVIFTPSTKA AVGDHDENIS FEECGRIIGK ELAEEVRDKA VRLYTEAAEY AKSRGIIICD TKFEFGLDEE GTLTLMDEVL TPDSSRFWPA DQYEVGINPP SFDKQFVRDW LEQSGWNKKA PAPKVPADVI RKTVEKYQEA LTLLTQD

NT Sequence

ATGAGTGAGA TTGGTTTGGT AAAGATTTAT TCCGGAAAAG TGCGCGATTT ATATGAAATC GACGATAAAC GTATGCTGAT GGTCGCTTCC GACCGCCTGT CCGCGTTTGA TGTGATTTTG GACGCCCCGA TTCCGGGTAA GGGCGAAATC CTGACGCAGA TTTCCAATTT TTGGTTTAAA AAACTGGCGC ATATTATGCC CAACCATTTC ACCGGCGATA CCGTGTACGA TGTTTTGCCT GAAAACGAAG CCAAAGCTTT GGAAAAACGC GCCGTTGTAG CTAAAAAACT CACTCCGGTG AAAGTGGAGG CCATTGTGCG CGGTTATCTG GCAGGCAGCG GTTGGAAAGA TTATCAAAAA ACCGGCTCGG TTTGCGGTAT TCGGCTGCCC GAAGGTATGC GGGAAGCGCA ACAACTACCC GAAGTGATTT TTACGCCCTC AACTAAAGCC GCAGTCGGCG ATCACGATGA AAACATCAGC TTTGAAGAAT GCGGACGCAT TATCGGTAAA GAATTGGCGG AAGAAGTGCG CGACAAAGCG GTTCGGCTTT ACACCGAAGC GGCGGAATAT GCCAAATCGC GCGGCATCAT CATTTGCGAT ACCAAATTTG AATTCGGTTT GGATGAAGAA GGTACGCTGA CGCTGATGGA TGAGGTATTG ACTCCCGATT CGAGCCGCTT CTGGCCGGCG GATCAATATG AAGTCGGCAT CAATCCGCCG TCTTTTGACA AGCAATTCGT CCGCGACTGG CTGGAACAAA GCGGTTGGAA CAAAAAAGCC CCTGCGCCTA AAGTGCCTGC CGATGTAATT CGGAAAACGG TCGAGAAATA TCAGGAAGCA TTGACTTTAC TGACACAAGA TTGA

Details for NegoA.01068.a.B1.GE40043

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	33kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS EIGLVKIYSG KVRDLYEIDD KRMLMVASDR LSAFDVILDA PIPGKGEILT QISNFWFKKL AHIMPNHFTG DTVYDVLPEN EAKALEKRAV VAKKLTPVKV EAIVRGYLAG SGWKDYQKTG SVCGIRLPEG MREAQQLPEV IFTPSTKAAV GDHDENISFE ECGRIIGKEL AEEVRDKAVR LYTEAAEYAK SRGIIICDTK FEFGLDEEGT LTLMDEVLTP DSSRFWPADQ YEVGTNPPSF DKQFVRDWLE QSGWNKKAPA PKVPADVIRK TVEKYQEXXT LLTQD

Validated NT Sequence

atcttactca cttaatcttg tgtcagtaaa gtnnnnnttt cctgatattt ctcgaccgtt ttccgaatta catcggcagg cactttaggc gcaggggctt ttttgttcca accgctttgt tccagccagt cgcggacgaa ttgtttgtca aaagacggcg gattggtgcc gacttcatat tgatccgccg gccagaagcg gctcgaatcg ggagtcaata cctcatccat cagcgtcagc gtaccttctt catccaaacc gaattcaaat ttggtatcgc aaatgatgat gccgcgcgat ttggcatatt ccgccgcttc ggtgtaaagc cgaaccgctt tgtcgcgcac ttcttccgcc aattctttac cgataatgcg tccgcattct tcaaagctga tgttttcatc gtgatcgccg actgcggctt tagttgaggg cgtaaaaatc acttcgggta gttgttgcgc ttcccgcata ccttcgggca gccgaatacc gcaaaccgag ccggtttttt gataatcttt ccaaccgctg cctgccagat aaccgcgcac aatggcctcc actttcaccg gagtgagttt tttagctaca acggcgcgtt tttccaaagc tttggcttcg ttttcaggca aaacatcgta cacggtatcg ccggtgaaat ggttgggcat aatatgcgcc agttttttaa accaaaaatt ggaaatctgc gtcaggattt cgcccttacc cggaatcggg gcgtccaaaa tcacatcaaa cgcggacagg cggtcggaag cgaccatcag catacgttta tcgtcgattt catataaatc gcgcactttt ccggaataaa tctttaccaa accaatctca ctcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMS EIGLVKIYSG KVRDLYEIDD KRMLMVASDR LSAFDVILDA PIPGKGEILT QISNFWFKKL AHIMPNHFTG DTVYDVLPEN EAKALEKRAV VAKKLTPVKV EAIVRGYLAG SGWKDYQKTG SVCGIRLPEG MREAQQLPEV IFTPSTKAAV GDHDENISFE ECGRIIGKEL AEEVRDKAVR LYTEAAEYAK SRGIIICDTK FEFGLDEEGT LTLMDEVLTP DSSRFWPADQ YEVGINPPSF DKQFVRDWLE QSGWNKKAPA PKVPADVIRK TVEKYQEALT LLTQD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg agattggttt ggtaaagatt tattccggaa aagtgcgcga tttatatgaa atcgacgata aacgtatgct gatggtcgct tccgaccgcc tgtccgcgtt tgatgtgatt ttggacgccc cgattccggg taagggcgaa atcctgacgc agatttccaa tttttggttt aaaaaactgg cgcatattat gcccaaccat ttcaccggcg ataccgtgta cgatgttttg cctgaaaacg aagccaaagc tttggaaaaa cgcgccgttg tagctaaaaa actcactccg gtgaaagtgg aggccattgt gcgcggttat ctggcaggca gcggttggaa agattatcaa aaaaccggct cggtttgcgg tattcggctg cccgaaggta tgcgggaagc gcaacaacta cccgaagtga tttttacgcc ctcaactaaa gccgcagtcg gcgatcacga tgaaaacatc agctttgaag aatgcggacg cattatcggt aaagaattgg cggaagaagt gcgcgacaaa gcggttcggc tttacaccga agcggcggaa tatgccaaat cgcgcggcat catcatttgc gataccaaat ttgaattcgg tttggatgaa gaaggtacgc tgacgctgat ggatgaggta ttgactcccg attcgagccg cttctggccg gcggatcaat atgaagtcgg catcaatccg ccgtcttttg acaagcaatt cgtccgcgac tggctggaac aaagcggttg gaacaaaaaa gcccctgcgc ctaaagtgcc tgccgatgta attcggaaaa cggtcgagaa atatcaggaa gcattgactt tactgacaca agattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for NegoA.01068.a.B1.PS37960

PURIFICATION DATe:	6/28/2016
CONCENTRATION:	48.91mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMS EIGLVKIYSG KVRDLYEIDD KRMLMVASDR LSAFDVILDA PIPGKGEILT QISNFWFKKL AHIMPNHFTG DTVYDVLPEN EAKALEKRAV VAKKLTPVKV EAIVRGYLAG SGWKDYQKTG SVCGIRLPEG MREAQQLPEV IFTPSTKAAV GDHDENISFE ECGRIIGKEL AEEVRDKAVR LYTEAAEYAK SRGIIICDTK FEFGLDEEGT LTLMDEVLTP DSSRFWPADQ YEVGINPPSF DKQFVRDWLE QSGWNKKAPA PKVPADVIRK TVEKYQEALT LLTQD

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTG AGATTGGTTT GGTAAAGATT TATTCCGGAA AAGTGCGCGA TTTATATGAA ATCGACGATA AACGTATGCT GATGGTCGCT TCCGACCGCC TGTCCGCGTT TGATGTGATT TTGGACGCCC CGATTCCGGG TAAGGGCGAA ATCCTGACGC AGATTTCCAA TTTTTGGTTT AAAAAACTGG CGCATATTAT GCCCAACCAT TTCACCGGCG ATACCGTGTA CGATGTTTTG CCTGAAAACG AAGCCAAAGC TTTGGAAAAA CGCGCCGTTG TAGCTAAAAA ACTCACTCCG GTGAAAGTGG AGGCCATTGT GCGCGGTTAT CTGGCAGGCA GCGGTTGGAA AGATTATCAA AAAACCGGCT CGGTTTGCGG TATTCGGCTG CCCGAAGGTA TGCGGGAAGC GCAACAACTA CCCGAAGTGA TTTTTACGCC CTCAACTAAA GCCGCAGTCG GCGATCACGA TGAAAACATC AGCTTTGAAG AATGCGGACG CATTATCGGT AAAGAATTGG CGGAAGAAGT GCGCGACAAA GCGGTTCGGC TTTACACCGA AGCGGCGGAA TATGCCAAAT CGCGCGGCAT CATCATTTGC GATACCAAAT TTGAATTCGG TTTGGATGAA GAAGGTACGC TGACGCTGAT GGATGAGGTA TTGACTCCCG ATTCGAGCCG CTTCTGGCCG GCGGATCAAT ATGAAGTCGG CATCAATCCG CCGTCTTTTG ACAAGCAATT CGTCCGCGAC TGGCTGGAAC AAAGCGGTTG GAACAAAAAA GCCCCTGCGC CTAAAGTGCC TGCCGATGTA ATTCGGAAAA CGGTCGAGAA ATATCAGGAA GCATTGACTT TACTGACACA AGATTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT