SSGCID: Target Page

NegoA.01050.a

3.4-dihydroxy-2-butanone 4-phosphate synthase

CENTER ID:	NegoA.01050.a
ORGANISM:	Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NegoA.01050.a.B1.GE40041	full length		1	445

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|521006.8.peg.1278
UniProt:	B4RM34

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDTAGLRRR NLRQWIAEHY GGLQNRFAEA VALNTGELSA LLKNKSFGEK KARKIEQAAK MPAFWLDTEH TGRPPGHTGK HTMSHISPIP EILADIKAGK MVIITDAEDR ENEGDLLMAA QFVTPEAVNF MIKHARGLVC LPMEGAMVEK LGLPMMTQKN GAQYGTNFTV SIEAAHGITT GISAADRALT IQTAVSPTAK PADIVQPGHI FPLRAQKGGV LVRAGHTEAG VDLAQMNGLI PAAVICEIIN DDGTMARMPE LMKFAEEHKL KIGTIADLIE YRSRTESLLE DMGDAPVQTP WGEFQQHVYV DKLSGETHLA LVKGTPSADT ETLVRVHEPF SVMDFIQANP RHSWSLPKAL ERVQQAESGV VILLHHTEDG ASLLDRTLPK GANQAYKWDS KSYGIGAQIL AGLHVKKLRV LGQPSSFTGL TGFGLEVVGF EEAEK

NT Sequence

ATGACCGACA CAGCCGGCTT GCGCCGCCGC AACCTGCGGC AATGGATAGC GGAACACTAC GGCGGACTGC AAAACCGTTT TGCCGAAGCC GTTGCCCTCA ACACAGGCGA ACTCTCCGCC CTTTTGAAAA ACAAATCCTT CGGCGAGAAA AAAGCCCGCA AAATCGAACA GGCGGCAAAA ATGCCCGCCT TCTGGCTCGA TACCGAACAC ACCGGCCGAC CGCCCGGACA CACAGGAAAA CACACCATGT CCCATATTTC CCCCATCCCC GAAATCCTAG CCGACATCAA AGCCGGTAAA ATGGTCATCA TCACCGATGC CGAAGACCGC GAAAACGAAG GCGACCTGCT GATGGCGGCG CAATTCGTTA CCCCCGAAGC CGTCAACTTT ATGATCAAAC ACGCGCGCGG CTTAGTCTGC CTGCCTATGG AAGGTGCAAT GGTCGAAAAA CTCGGTCTGC CGATGATGAC CCAAAAAAAC GGCGCGCAAT ACGGTACCAA CTTTACCGTT TCCATCGAAG CCGCGCACGG CATTACCACC GGCATTTCCG CCGCCGACCG CGCCCTGACT ATTCAAACCG CCGTTTCCCC GACCGCCAAA CCCGCAGACA TCGTCCAACC CGGTCATATC TTCCCGCTTC GCGCCCAAAA AGGCGGCGTA CTCGTCCGTG CCGGACACAC CGAAGCCGGC GTCGACTTGG CGCAAATGAA CGGGCTGATT CCCGCCGCCG TCATCTGCGA AATCATCAAC GACGACGGCA CGATGGCGCG TATGCCCGAA CTGATGAAAT TCGCCGAAGA ACACAAGCTC AAAATCGGCA CGATTGCCGA CCTCATCGAA TACCGCAGCC GCACCGAAAG CCTGCTCGAA GATATGGGCG ACGCGCCCGT ACAAACTCCG TGGGGCGAGT TCCAACAACA CGTTTACGTC GACAAACTCT CCGGCGAAAC CCACCTCGCC CTCGTCAAAG GCACACCCTC CGCCGACACC GAAACCCTCG TCCGCGTCCA CGAACCCTTC AGCGTGATGG ACTTCATCCA AGCCAATCCG CGCCATTCCT GGTCGCTGCC CAAAGCCCTT GAGCGCGTCC AACAGGCCGA AAGCGGCGTC GTCATCCTCC TGCACCACAC CGAAGACGGC GCATCCCTGC TCGACCGCAC CCTACCCAAA GGCGCAAACC AAGCCTACAA ATGGGACAGC AAAAGCTACG GCATCGGCGC GCAAATCCTC GCCGGCCTCC ACGTCAAAAA ACTGCGCGTC CTCGGCCAAC CGTCATCTTT CACCGGCCTC ACCGGCTTCG GCCTCGAAGT CGTCGGCTTT GAAGAAGCGG AAAAATAA

Details for NegoA.01050.a.B1.GE40041

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMT DTAGLRRRNL RQWIAEHYGG LQNRFAEAVA LNTGELSALL KNKSFGEKKA RKIEQAAKMP AFWLDTEHTG RPPGHTGKHT MSHISPIPEI LADIKAGKMV IITDAEDREN EGDLLMAAQF VTPEAVNFMI KHARGLVCLP MEGAMVEKLG LPMMTQKNGA QYGTNFTVSI EAAHGITTGI SAADRALTIQ TAVSPTAKPA DIVQPGHIFP LRAQKGGVLV RAGHTEAGVD LAQMNGLIPA AVICEIINDD GTMARMPELM KFAEEHKLKI GTIADLIEYR SRTESLLEDM GDAPVQTPWG EFQQHVYVDK LSGETHLALV KGTPSADTET LVRVHEPFSV MDFIQANPRH SWSLPKALER VQQAESGVVI LLHHTEDGAS LLDRTLPKGA NQAYKWDSKS YGIGAQILAG LHVKKLRVLG QPSSFTGLTG FGLE

Validated NT Sequence

acttcgaggc cgaagccggt gaggccggtg aaagatgacg gttggccgag gacgcgcagt tttttgacgt ggaggccggc gaggatttgc gcgccgatgc cgtagctttt gctgtcccat ttgtaggctt ggtttgcgcc tttgggtagg gtgcggtcga gcagggatgc gccgtcttcg gtgtggtgca ggaggatgac gacgccgctt tcggcctgtt ggacgcgctc aagggctttg ggcagcgacc aggaatggcg cggattggct tggatgaagt ccatcacgct gaagggttcg tggacacgga cgagggtttc ggtgtcggcg gagggtgtgc ctttgacgag ggcgaggtgg gtttcgccgg agagtttgtc gacgtaaacg tgttgttgga actcgcccca cggagtttgt acgggcgcgt cgcccatatc ttcgagcagg ctttcggtgc ggctgcggta ttcgatgagg tcggcaatcg tgccgatttt gagcttgtgt tcttcggcga atttcatcag ttcgggcata cgcgccatcg tgccgtcgtc gttgatgatt tcgcagatga cggcggcggg aatcagcccg ttcatttgcg ccaagtcgac gccggcttcg gtgtgtccgg cacggacgag tacgccgcct ttttgggcgc gaagcgggaa gatatgaccg ggttggacga tgtctgcggg tttggcggtc ggggaaacgg cggtttgaat agtcagggcg cggtcggcgg cggaaatgcc ggtggtaatg ccgtgcgcgg cttcgatgga aacggtaaag ttggtaccgt attgcgcgcc gtttttttgg gtcatcatcg gcagaccgag tttttcgacc attgcacctt ccataggcag gcagactaag ccgcgcgcgt gtttgatcat aaagttgacg gcttcggggg taacgaattg cgccgccatc agcaggtcgc cttcgttttc gcggtcttcg gcatcggtga tgatgaccat tttaccggct ttgatgtcgg ctaggatttc ggggatgggg gaaatatggg acatggtgtg ttttcctgtg tgtccgggcg gtcggccggt gtgttcggta tcgagccaga aggcgggcat ttttgccgcc tgttcgattt tgcgggcttt tttctcgccg aaggatttgt ttttcaaaag ggcggagagt tcgcctgtgt tgagggcaac ggcttcggca aaacggtttt gcagtccgcc gtagtgttcc gctatccatt gccgcaggtt gcggcggcgc aagccggctg tgtcggtcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg acacagccgg cttgcgccgc cgcaacctgc ggcaatggat agcggaacac tacggcggac tgcaaaaccg ttttgccgaa gccgttgccc tcaacacagg cgaactctcc gcccttttga aaaacaaatc cttcggcgag aaaaaagccc gcaaaatcga acaggcggca aaaatgcccg ccttctggct cgataccgaa cacaccggcc gaccgcccgg acacacagga aaacacacca tgtcccatat ttcccccatc cccgaaatcc tagccgacat caaagccggt aaaatggtca tcatcaccga tgccgaagac cgcgaaaacg aaggcgacct gctgatggcg gcgcaattcg ttacccccga agccgtcaac tttatgatca aacacgcgcg cggcttagtc tgcctgccta tggaaggtgc aatggtcgaa aaactcggtc tgccgatgat gacccaaaaa aacggcgcgc aatacggtac caactttacc gtttccatcg aagccgcgca cggcattacc accggcattt ccgccgccga ccgcgccctg actattcaaa ccgccgtttc cccgaccgcc aaacccgcag acatcgtcca acccggtcat atcttcccgc ttcgcgccca aaaaggcggc gtactcgtcc gtgccggaca caccgaagcc ggcgtcgact tggcgcaaat gaacgggctg attcccgccg ccgtcatctg cgaaatcatc aacgacgacg gcacgatggc gcgtatgccc gaactgatga aattcgccga agaacacaag ctcaaaatcg gcacgattgc cgacctcatc gaataccgca gccgcaccga aagcctgctc gaagatatgg gcgacgcgcc cgtacaaact ccgtggggcg agttccaaca acacgtttac gtcgacaaac tctccggcga aacccacctc gccctcgtca aaggcacacc ctccgccgac accgaaaccc tcgtccgcgt ccacgaaccc ttcagcgtga tggacttcat ccaagccaat ccgcgccatt cctggtcgct gcccaaagcc cttgagcgcg tccaacaggc cgaaagcggc gtcgtcatcc tcctgcacca caccgaagac ggcgcatccc tgctcgaccg caccctaccc aaaggcgcaa accaagccta caaatgggac agcaaaagct acggcatcgg cgcgcaaatc ctcgccggcc tccacgtcaa aaaactgcgc gtcctcggcc aaccgtcatc tttcaccggc ctcaccggct tcggcctcga agtcgtcggc tttgaagaag cggaaaaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat