NegoA.01032.a
tyrosyl-tRNA synthetase
| CENTER ID: | NegoA.01032.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.01032.a.B1.GE40040 | full length | 1 | 431 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.01032.a.B1.PW37921 | full length | 1 | 431 |
Structures
6OTJ
DEPOSITED: 5/3/2019
DETERMINATION: XRay
CLONE: NegoA.01032.a.B1.GE40040
PROTEIN: NegoA.01032.a.B1.PW37921
Molecular models
| METHOD | RESULTS |
|---|---|
| comparative modelling | Robetta_85041 |
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.104 |
| UniProt: | B4RP13 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVIQDLQSR GLIAQTTDIE ALDALLNEQK IALYCGFDPT ADSLHIGHLL PVLALRRFQQ AGHTPIALVG GATGMIGDPS FKAAERSLNS AETVAGWVGS IRSQLTPFLS FEGGNAAIMA NNADWFGSMN CLDFLRDIGK HFSVNAMLNK ESVKQRIDRD GAGISFTEFA YSLLQGYDFA ELNKRHGAVL EIGGSDQWGN ITAGIDLTRR LNQKQVFGLT LPLVTKSDGT KFGKTEGGAV WLNAKKTSPY QFYQFWLKVA DADVYKFLKY FTFLSIEEIG VVEAKDKASG SKPEAQRILA EEMTRLIHGE EALAAAQRIS ESLFAEDQSR LTESDFEQLA LDGLPAFEVS DGINAVEALV KTGLAASNKE ARGFVNAKAV LLNGKPAEAN NPNHAAERPD DAYLLIGEYK RFGKYTILRR GKRNHALLVW K
NT Sequence
ATGAGCGTCA TCCAAGACCT GCAATCGCGC GGCCTTATCG CGCAAACCAC CGACATCGAA GCCTTAGACG CTTTGCTGAA CGAACAAAAA ATCGCCCTTT ACTGCGGTTT CGACCCGACA GCCGACAGCC TGCACATCGG ACACCTGCTG CCCGTATTGG CATTGCGCCG CTTCCAACAG GCGGGGCATA CGCCGATTGC ACTGGTGGGC GGCGCGACCG GTATGATCGG CGACCCCAGC TTCAAAGCCG CCGAACGCAG CTTGAATTCC GCCGAAACCG TTGCCGGCTG GGTAGGAAGC ATACGCAGCC AATTAACCCC TTTCTTGAGC TTTGAAGGCG GAAACGCCGC CATTATGGCG AACAATGCCG ACTGGTTCGG CAGCATGAAC TGCCTCGACT TCCTGCGCGA CATCGGCAAG CATTTCTCCG TCAACGCCAT GCTGAACAAA GAATCCGTCA AACAGCGCAT CGACCGCGAC GGCGCAGGCA TTTCCTTTAC CGAGTTCGCC TATTCCCTGC TGCAAGGCTA CGACTTCGCC GAGTTGAACA AACGCCACGG CGCGGTTTTG GAAATCGGCG GTTCCGACCA GTGGGGCAAC ATCACCGCCG GTATCGACCT GACCCGCCGC CTGAACCAAA AACAAGTGTT CGGTCTGACC CTGCCTTTGG TTACCAAATC CGACGGTACC AAATTCGGCA AAACCGAAGG CGGCGCGGTG TGGCTGAACG CGAAAAAAAC CTCGCCGTAC CAGTTCTACC AGTTCTGGCT GAAAGTCGCC GATGCCGATG TGTATAAATT CCTGAAATAC TTTACCTTCC TGTCCATCGA AGAAATCGGT GTCGTCGAAG CCAAAGACAA GGCAAGCGGC AGCAAGCCCG AAGCGCAACG CATCCTCGCC GAAGAAATGA CCCGCCTGAT TCACGGCGAA GAAGCCCTTG CCGCCGCGCA ACGCATTTCC GAAAGCCTGT TTGCCGAAGA CCAAAGCCGG CTTACCGAAA GCGACTTCGA GCAGCTCGCC CTCGACGGCC TGCCTGCATT TGAAGTTTCA GACGGCATCA ACGCCGTCGA AGCCTTGGTC AAAACCGGCT TGGCAGCGTC CAACAAAGAA GCGCGCGGCT TTGTCAATGC CAAAGCGGTT CTGCTCAACG GCAAACCGGC TGAAGCCAAC AACCCCAACC ACGCCGCCGA ACGCCCCGAC GATGCCTATC TGTTGATAGG CGAATACAAA CGTTTCGGCA AATACACCAT CCTCCGGCGC GGCAAACGCA ACCACGCGCT TTTGGTTTGG AAATAA
Details for NegoA.01032.a.B1.GE40040
| HARVESTED ON: | 3/24/2016 |
| SEQUENCED ON: | 3/25/2016 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMS VIQDLQSRGL IAQTTDIEAL DALLNEQKIA LYCGFDPTAD SLHIGHLLPV LALRRFQQAG HTPIALVGGA TGMIGDPSFK AAERSLNSAE TVAGWVGSIR SQLTPFLSFE GGNAAIMANN ADWFGSMNCL DFLRDIGKHF SVNAMLNKES VKQRIDRDGA GISFTEFAYS LLQGYDFAEL NKRHGAVLEI GGSDQWGNIT AGIDLTRRLN QKQVFGLTLP LVTKSDGTKF GKTEGGAVWL NAKKTSPYQF YQFWLKVADA DVYKFLKYFT FLSIEEIGVV EAKDKASGSK PEAQRILAEE MTRLIHGEEA LAAAQRISES LFAEDQSRLT ESDFEQLALD GLPAFEVSDG INAVEALVKT GLAASNKEAR GFVNAKAVLL NGKPAEANNP NHAAERPDDA YLLIGEYKRF GKYTILRRGK
Validated NT Sequence
gtttgccgcg ccggaggatg gtgtatttgc cgaaacgttt gtattcgcct atcaacagat aggcatcgtc ggggcgttcg gcggcgtggt tggggttgtt ggcttcagcc ggtttgccgt tgagcagaac cgctttggca ttgacaaagc cgcgcgcttc tttgttggac gctgccaagc cggttttgac caaggcttcg acggcgttga tgccgtctga aacttcaaat gcaggcaggc cgtcgagggc gagctgctcg aagtcgcttt cggtaagccg gctttggtct tcggcaaaca ggctttcgga aatgcgttgc gcggcggcaa gggcttcttc gccgtgaatc aggcgggtca tttcttcggc gaggatgcgt tgcgcttcgg gcttgctgcc gcttgccttg tctttggctt cgacgacacc gatttcttcg atggacagga aggtaaagta tttcaggaat ttatacacat cggcatcggc gactttcagc cagaactggt agaactggta cggcgaggtt tttttcgcgt tcagccacac cgcgccgcct tcggttttgc cgaatttggt accgtcggat ttggtaacca aaggcagggt cagaccgaac acttgttttt ggttcaggcg gcgggtcagg tcgataccgg cggtgatgtt gccccactgg tcggaaccgc cgatttccaa aaccgcgccg tggcgtttgt tcaactcggc gaagtcgtag ccttgcagca gggaataggc gaactcggtg aaggaaatgc ctgcgccgtc gcggtcgatg cgctgtttga cggattcttt gttcagcatg gcgttgacgg agaaatgctt gccgatgtcg cgcaggaagt cgaggcagtt catgctgccg aaccagtcgg cattgttcgc cataatggcg gcgtttccgc cttcaaagct caagaaaggg gttaattggc tgcgtatgct tcctacccag ccggcaacgg tttcggcgga attcaagctg cgttcggcgg ctttgaagct ggggtcgccg atcataccgg tcgcgccgcc caccagtgca atcggcgtat gccccgcctg ttggaagcgg cgcaatgcca atacgggcag caggtgtccg atgtgcaggc tgtcggctgt cgggtcgaaa ccgcagtaaa gggcgatttt ttgttcgttc agcaaagcgt ctaaggcttc gatgtcggtg gtttgcgcga taaggccgcg cgattgcagg tcttggatga cgctcatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS VIQDLQSRGL IAQTTDIEAL DALLNEQKIA LYCGFDPTAD SLHIGHLLPV LALRRFQQAG HTPIALVGGA TGMIGDPSFK AAERSLNSAE TVAGWVGSIR SQLTPFLSFE GGNAAIMANN ADWFGSMNCL DFLRDIGKHF SVNAMLNKES VKQRIDRDGA GISFTEFAYS LLQGYDFAEL NKRHGAVLEI GGSDQWGNIT AGIDLTRRLN QKQVFGLTLP LVTKSDGTKF GKTEGGAVWL NAKKTSPYQF YQFWLKVADA DVYKFLKYFT FLSIEEIGVV EAKDKASGSK PEAQRILAEE MTRLIHGEEA LAAAQRISES LFAEDQSRLT ESDFEQLALD GLPAFEVSDG INAVEALVKT GLAASNKEAR GFVNAKAVLL NGKPAEANNP NHAAERPDDA YLLIGEYKRF GKYTILRRGK RNHALLVWK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg tcatccaaga cctgcaatcg cgcggcctta tcgcgcaaac caccgacatc gaagccttag acgctttgct gaacgaacaa aaaatcgccc tttactgcgg tttcgacccg acagccgaca gcctgcacat cggacacctg ctgcccgtat tggcattgcg ccgcttccaa caggcggggc atacgccgat tgcactggtg ggcggcgcga ccggtatgat cggcgacccc agcttcaaag ccgccgaacg cagcttgaat tccgccgaaa ccgttgccgg ctgggtagga agcatacgca gccaattaac ccctttcttg agctttgaag gcggaaacgc cgccattatg gcgaacaatg ccgactggtt cggcagcatg aactgcctcg acttcctgcg cgacatcggc aagcatttct ccgtcaacgc catgctgaac aaagaatccg tcaaacagcg catcgaccgc gacggcgcag gcatttcctt taccgagttc gcctattccc tgctgcaagg ctacgacttc gccgagttga acaaacgcca cggcgcggtt ttggaaatcg gcggttccga ccagtggggc aacatcaccg ccggtatcga cctgacccgc cgcctgaacc aaaaacaagt gttcggtctg accctgcctt tggttaccaa atccgacggt accaaattcg gcaaaaccga aggcggcgcg gtgtggctga acgcgaaaaa aacctcgccg taccagttct accagttctg gctgaaagtc gccgatgccg atgtgtataa attcctgaaa tactttacct tcctgtccat cgaagaaatc ggtgtcgtcg aagccaaaga caaggcaagc ggcagcaagc ccgaagcgca acgcatcctc gccgaagaaa tgacccgcct gattcacggc gaagaagccc ttgccgccgc gcaacgcatt tccgaaagcc tgtttgccga agaccaaagc cggcttaccg aaagcgactt cgagcagctc gccctcgacg gcctgcctgc atttgaagtt tcagacggca tcaacgccgt cgaagccttg gtcaaaaccg gcttggcagc gtccaacaaa gaagcgcgcg gctttgtcaa tgccaaagcg gttctgctca acggcaaacc ggctgaagcc aacaacccca accacgccgc cgaacgcccc gacgatgcct atctgttgat aggcgaatac aaacgtttcg gcaaatacac catcctccgg cgcggcaaac gcaaccacgc gcttttggtt tggaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NegoA.01032.a.B1.PW37921
| PURIFICATION DATe: | 6/13/2016 |
| CONCENTRATION: | 26mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS VIQDLQSRGL IAQTTDIEAL DALLNEQKIA LYCGFDPTAD SLHIGHLLPV LALRRFQQAG HTPIALVGGA TGMIGDPSFK AAERSLNSAE TVAGWVGSIR SQLTPFLSFE GGNAAIMANN ADWFGSMNCL DFLRDIGKHF SVNAMLNKES VKQRIDRDGA GISFTEFAYS LLQGYDFAEL NKRHGAVLEI GGSDQWGNIT AGIDLTRRLN QKQVFGLTLP LVTKSDGTKF GKTEGGAVWL NAKKTSPYQF YQFWLKVADA DVYKFLKYFT FLSIEEIGVV EAKDKASGSK PEAQRILAEE MTRLIHGEEA LAAAQRISES LFAEDQSRLT ESDFEQLALD GLPAFEVSDG INAVEALVKT GLAASNKEAR GFVNAKAVLL NGKPAEANNP NHAAERPDDA YLLIGEYKRF GKYTILRRGK
Validated NT Sequence
gtttgccgcg ccggaggatg gtgtatttgc cgaaacgttt gtattcgcct atcaacagat aggcatcgtc ggggcgttcg gcggcgtggt tggggttgtt ggcttcagcc ggtttgccgt tgagcagaac cgctttggca ttgacaaagc cgcgcgcttc tttgttggac gctgccaagc cggttttgac caaggcttcg acggcgttga tgccgtctga aacttcaaat gcaggcaggc cgtcgagggc gagctgctcg aagtcgcttt cggtaagccg gctttggtct tcggcaaaca ggctttcgga aatgcgttgc gcggcggcaa gggcttcttc gccgtgaatc aggcgggtca tttcttcggc gaggatgcgt tgcgcttcgg gcttgctgcc gcttgccttg tctttggctt cgacgacacc gatttcttcg atggacagga aggtaaagta tttcaggaat ttatacacat cggcatcggc gactttcagc cagaactggt agaactggta cggcgaggtt tttttcgcgt tcagccacac cgcgccgcct tcggttttgc cgaatttggt accgtcggat ttggtaacca aaggcagggt cagaccgaac acttgttttt ggttcaggcg gcgggtcagg tcgataccgg cggtgatgtt gccccactgg tcggaaccgc cgatttccaa aaccgcgccg tggcgtttgt tcaactcggc gaagtcgtag ccttgcagca gggaataggc gaactcggtg aaggaaatgc ctgcgccgtc gcggtcgatg cgctgtttga cggattcttt gttcagcatg gcgttgacgg agaaatgctt gccgatgtcg cgcaggaagt cgaggcagtt catgctgccg aaccagtcgg cattgttcgc cataatggcg gcgtttccgc cttcaaagct caagaaaggg gttaattggc tgcgtatgct tcctacccag ccggcaacgg tttcggcgga attcaagctg cgttcggcgg ctttgaagct ggggtcgccg atcataccgg tcgcgccgcc caccagtgca atcggcgtat gccccgcctg ttggaagcgg cgcaatgcca atacgggcag caggtgtccg atgtgcaggc tgtcggctgt cgggtcgaaa ccgcagtaaa gggcgatttt ttgttcgttc agcaaagcgt ctaaggcttc gatgtcggtg gtttgcgcga taaggccgcg cgattgcagg tcttggatga cgctcatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMS VIQDLQSRGL IAQTTDIEAL DALLNEQKIA LYCGFDPTAD SLHIGHLLPV LALRRFQQAG HTPIALVGGA TGMIGDPSFK AAERSLNSAE TVAGWVGSIR SQLTPFLSFE GGNAAIMANN ADWFGSMNCL DFLRDIGKHF SVNAMLNKES VKQRIDRDGA GISFTEFAYS LLQGYDFAEL NKRHGAVLEI GGSDQWGNIT AGIDLTRRLN QKQVFGLTLP LVTKSDGTKF GKTEGGAVWL NAKKTSPYQF YQFWLKVADA DVYKFLKYFT FLSIEEIGVV EAKDKASGSK PEAQRILAEE MTRLIHGEEA LAAAQRISES LFAEDQSRLT ESDFEQLALD GLPAFEVSDG INAVEALVKT GLAASNKEAR GFVNAKAVLL NGKPAEANNP NHAAERPDDA YLLIGEYKRF GKYTILRRGK RNHALLVWK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG TCATCCAAGA CCTGCAATCG CGCGGCCTTA TCGCGCAAAC CACCGACATC GAAGCCTTAG ACGCTTTGCT GAACGAACAA AAAATCGCCC TTTACTGCGG TTTCGACCCG ACAGCCGACA GCCTGCACAT CGGACACCTG CTGCCCGTAT TGGCATTGCG CCGCTTCCAA CAGGCGGGGC ATACGCCGAT TGCACTGGTG GGCGGCGCGA CCGGTATGAT CGGCGACCCC AGCTTCAAAG CCGCCGAACG CAGCTTGAAT TCCGCCGAAA CCGTTGCCGG CTGGGTAGGA AGCATACGCA GCCAATTAAC CCCTTTCTTG AGCTTTGAAG GCGGAAACGC CGCCATTATG GCGAACAATG CCGACTGGTT CGGCAGCATG AACTGCCTCG ACTTCCTGCG CGACATCGGC AAGCATTTCT CCGTCAACGC CATGCTGAAC AAAGAATCCG TCAAACAGCG CATCGACCGC GACGGCGCAG GCATTTCCTT TACCGAGTTC GCCTATTCCC TGCTGCAAGG CTACGACTTC GCCGAGTTGA ACAAACGCCA CGGCGCGGTT TTGGAAATCG GCGGTTCCGA CCAGTGGGGC AACATCACCG CCGGTATCGA CCTGACCCGC CGCCTGAACC AAAAACAAGT GTTCGGTCTG ACCCTGCCTT TGGTTACCAA ATCCGACGGT ACCAAATTCG GCAAAACCGA AGGCGGCGCG GTGTGGCTGA ACGCGAAAAA AACCTCGCCG TACCAGTTCT ACCAGTTCTG GCTGAAAGTC GCCGATGCCG ATGTGTATAA ATTCCTGAAA TACTTTACCT TCCTGTCCAT CGAAGAAATC GGTGTCGTCG AAGCCAAAGA CAAGGCAAGC GGCAGCAAGC CCGAAGCGCA ACGCATCCTC GCCGAAGAAA TGACCCGCCT GATTCACGGC GAAGAAGCCC TTGCCGCCGC GCAACGCATT TCCGAAAGCC TGTTTGCCGA AGACCAAAGC CGGCTTACCG AAAGCGACTT CGAGCAGCTC GCCCTCGACG GCCTGCCTGC ATTTGAAGTT TCAGACGGCA TCAACGCCGT CGAAGCCTTG GTCAAAACCG GCTTGGCAGC GTCCAACAAA GAAGCGCGCG GCTTTGTCAA TGCCAAAGCG GTTCTGCTCA ACGGCAAACC GGCTGAAGCC AACAACCCCA ACCACGCCGC CGAACGCCCC GACGATGCCT ATCTGTTGAT AGGCGAATAC AAACGTTTCG GCAAATACAC CATCCTCCGG CGCGGCAAAC GCAACCACGC GCTTTTGGTT TGGAAATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T