NegoA.01026.a

glutamate-1-semialdehyde 2.1-aminomutase

CENTER ID: NegoA.01026.a
ORGANISM: Neisseria gonorrhoeae NCCP11945
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I/II

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NegoA.01026.a.B1.GE40039 full length 1 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|521006.8.peg.61
UniProt: B4RNX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRNEILFDR AKAVIPGGVN SPVRAFGSVG GVPRFIKKAE GAYVWDENGT RYTDYVGSWG PAIVGHAHPE VVEAVREAAL GGLSFGAPTE GEIAIAEQIA EIMPSVERLR LVSSGTEATM TAIRLARGFT GRDKIIKFEG CYHGHSDSLL VKAGSGLLTF GNPSSAGVPA DFTKHTLVLE YNNIAQLEEA FAQSGDEIAC VIVEPFVGNM NLVRPTEAFV KALRGLTEKH GAVLIYDEVM TGFRVALGGA QSLHGITPDL TTMGKVIGGG MPLAAFGGRK DIMECISPLG GVYQAGTLSG NPIAVAAGLK TLEIIQREGF YENLTALTQR LANGIAAAKA HGIEFAADSV GGMFGLYFAA HVPRNYADMA RSNIDAFKRF FHGMLDRGIA FGPSAYEAGF VSAAHTPELI DETVAVAVEV FKAMAA
NT Sequence
ATGAACCGTA ACGAAATTTT ATTCGACCGC GCCAAGGCGG TCATTCCCGG CGGCGTGAAT TCGCCCGTGC GCGCATTCGG CAGCGTCGGC GGCGTGCCGC GCTTCATCAA AAAAGCCGAA GGCGCGTATG TTTGGGACGA AAACGGCACG CGCTACACCG ATTATGTCGG CTCTTGGGGG CCTGCGATTG TCGGACACGC GCATCCCGAA GTCGTCGAAG CCGTGCGCGA AGCTGCGTTG GGCGGTTTGT CGTTCGGCGC GCCCACCGAA GGCGAAATCG CCATTGCCGA ACAAATTGCC GAAATTATGC CGTCTGTCGA ACGGCTGCGC CTCGTCAGCT CCGGCACGGA AGCGACGATG ACTGCCATCC GTCTGGCACG CGGTTTTACC GGCCGCGACA AAATCATCAA ATTTGAAGGC TGCTACCACG GCCATTCCGA CAGCCTGTTG GTGAAAGCAG GCAGCGGTCT GCTTACCTTC GGCAATCCTT CTTCCGCCGG TGTGCCTGCC GACTTTACCA AACATACTTT GGTACTCGAA TACAACAACA TCGCCCAACT CGAAGAAGCC TTTGCCCAAA GCGGCGACGA AATCGCCTGC GTGATTGTCG AACCCTTCGT CGGCAATATG AACCTCGTCC GCCCGACCGA AGCCTTTGTC AAAGCCTTGC GCGGATTGAC CGAAAAACAC GGCGCGGTGT TGATTTACGA CGAAGTGATG ACCGGTTTCC GCGTCGCGCT CGGCGGCGCG CAGTCGCTGC ACGGCATCAC GCCCGACCTG ACCACGATGG GCAAAGTCAT CGGCGGCGGT ATGCCGCTTG CCGCGTTCGG CGGACGCAAA GACATCATGG AATGTATTTC CCCGTTGGGC GGCGTGTATC AGGCAGGTAC ATTATCAGGC AACCCGATTG CCGTCGCCGC CGGCTTGAAA ACGCTGGAAA TCATCCAGCG CGAAGGCTTC TATGAAAACC TGACCGCCTT GACACAACGC CTTGCCAACG GTATTGCCGC CGCCAAAGCG CACGGTATCG AGTTTGCCGC CGACAGCGTG GGCGGTATGT TCGGTCTGTA TTTCGCCGCA CACGTGCCGC GAAACTATGC CGATATGGCG CGCTCCAATA TCGACGCTTT CAAACGCTTC TTCCACGGCA TGCTCGACCG CGGCATTGCC TTCGGCCCGT CCGCTTATGA AGCAGGTTTC GTTTCCGCCG CGCATACGCC CGAGCTGATT GACGAAACGG TTGCGGTTGC GGTTGAAGTG TTCAAGGCGA TGGCTGCATG A
Details for NegoA.01026.a.B1.GE40039
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMN RNEILFDRAK AIIPGGVNSP VRAFGSVGGV PRFIKKAEGA YVWDENGTRY TDYVGSWGPA IVGHAHPEVV EAVREAALGG LSFGAPTEGE IAIAEQIAEI MPSVERLRLV SSGTEATMTA IRLARGFTGR DKIIKFEGCY HGHSDSLLVK AGSGLLTFGN PSSAGVPADF TKHTLVLEYN NIAQLEEAFA QSGDEIACVI VEPFVGNMNL VRPTEAFVKA LRGLTEKHGA VLIYDEVMTG FRVALGGAQS LHGITPDLTT MGKVIGGGMP LAAFGGRKDI MECISPLGGV YQAGTLSGNP IAVAAGLKTL EIIQREGFYE NLTALTQRLA NGIAAAKAHG IEFAADSVGG MFGLYFAAHV PRNYADMARS NIDAFKRFFH GMLDRGIAFG PSAYEAGFVS AAHTPELIDE TVAVA
Validated NT Sequence
ccgcaaccgc aaccgtttcg tcaatcagct cgggcgtatg cgcggcggaa acgaaacccg cttcataagc ggacgggccg aaggcaatgc cgcggtcgag catgccgtgg aagaagcgtt tgaaagcgtc gatattggag cgcgccatat cggcatagtt tcgcggcacg tgtgcggcga aatacagacc gaacataccg cccacgctgt cggcggcaaa ctcgataccg tgcgctttgg cggcggcaat accgttggca aggcgttgtg tcaaggcggt caggttttca tagaagcctt cgcgctggat gatttccagc gttttcaagc cggcggcgac ggcaatcggg ttgcctgata atgtacctgc ctgatacacg ccgcccaacg gggaaataca ttccatgatg tctttgcgtc cgccgaacgc ggcaagcggc ataccgccgc cgatgacttt gcccatcgtg gtcaggtcgg gcgtgatgcc gtgcagcgac tgcgcgccgc cgagcgcgac gcggaaaccg gtcatcactt cgtcgtaaat caacaccgcg ccgtgttttt cggtcaatcc gcgcaaggct ttgacaaagg cttcggtcgg gcggacgagg ttcatattgc cgacgaaggg ttcgacaatc acgcaggcga tttcgtcgcc gctttgggca aaggcttctt cgagttgggc gatgttgttg tattcgagta ccaaagtatg tttggtaaag tcggcaggca caccggcgga agaaggattg ccgaaggtaa gcagaccgct gcctgctttc accaacaggc tgtcggaatg gccgtggtag cagccttcaa atttgatgat tttgtcgcgg ccggtaaaac cgcgtgccag acggatggca gtcatcgtcg cttccgtgcc ggagctgacg aggcgcagcc gttcgacaga cggcataatt tcggcaattt gttcggcaat ggcgatttcg ccttcggtgg gcgcgccgaa cgacaaaccg cccaacgcag cttcgcgcac ggcttcgacg acttcgggat gcgcgtgtcc gacaatcgca ggcccccaag agccgacata atcggtgtag cgcgtgccgt tttcgtccca aacatacgcg ccttcggctt ttttgatgaa gcgcggcacg ccgccgacgc tgccgaatgc gcgcacgggc gaattcacgc cgccggggat gatggccttg gcgcggtcga ataaaatttc gttacggttc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMN RNEILFDRAK AVIPGGVNSP VRAFGSVGGV PRFIKKAEGA YVWDENGTRY TDYVGSWGPA IVGHAHPEVV EAVREAALGG LSFGAPTEGE IAIAEQIAEI MPSVERLRLV SSGTEATMTA IRLARGFTGR DKIIKFEGCY HGHSDSLLVK AGSGLLTFGN PSSAGVPADF TKHTLVLEYN NIAQLEEAFA QSGDEIACVI VEPFVGNMNL VRPTEAFVKA LRGLTEKHGA VLIYDEVMTG FRVALGGAQS LHGITPDLTT MGKVIGGGMP LAAFGGRKDI MECISPLGGV YQAGTLSGNP IAVAAGLKTL EIIQREGFYE NLTALTQRLA NGIAAAKAHG IEFAADSVGG MFGLYFAAHV PRNYADMARS NIDAFKRFFH GMLDRGIAFG PSAYEAGFVS AAHTPELIDE TVAVAVEVFK AMAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc gtaacgaaat tttattcgac cgcgccaagg cggtcattcc cggcggcgtg aattcgcccg tgcgcgcatt cggcagcgtc ggcggcgtgc cgcgcttcat caaaaaagcc gaaggcgcgt atgtttggga cgaaaacggc acgcgctaca ccgattatgt cggctcttgg gggcctgcga ttgtcggaca cgcgcatccc gaagtcgtcg aagccgtgcg cgaagctgcg ttgggcggtt tgtcgttcgg cgcgcccacc gaaggcgaaa tcgccattgc cgaacaaatt gccgaaatta tgccgtctgt cgaacggctg cgcctcgtca gctccggcac ggaagcgacg atgactgcca tccgtctggc acgcggtttt accggccgcg acaaaatcat caaatttgaa ggctgctacc acggccattc cgacagcctg ttggtgaaag caggcagcgg tctgcttacc ttcggcaatc cttcttccgc cggtgtgcct gccgacttta ccaaacatac tttggtactc gaatacaaca acatcgccca actcgaagaa gcctttgccc aaagcggcga cgaaatcgcc tgcgtgattg tcgaaccctt cgtcggcaat atgaacctcg tccgcccgac cgaagccttt gtcaaagcct tgcgcggatt gaccgaaaaa cacggcgcgg tgttgattta cgacgaagtg atgaccggtt tccgcgtcgc gctcggcggc gcgcagtcgc tgcacggcat cacgcccgac ctgaccacga tgggcaaagt catcggcggc ggtatgccgc ttgccgcgtt cggcggacgc aaagacatca tggaatgtat ttccccgttg ggcggcgtgt atcaggcagg tacattatca ggcaacccga ttgccgtcgc cgccggcttg aaaacgctgg aaatcatcca gcgcgaaggc ttctatgaaa acctgaccgc cttgacacaa cgccttgcca acggtattgc cgccgccaaa gcgcacggta tcgagtttgc cgccgacagc gtgggcggta tgttcggtct gtatttcgcc gcacacgtgc cgcgaaacta tgccgatatg gcgcgctcca atatcgacgc tttcaaacgc ttcttccacg gcatgctcga ccgcggcatt gccttcggcc cgtccgctta tgaagcaggt ttcgtttccg ccgcgcatac gcccgagctg attgacgaaa cggttgcggt tgcggttgaa gtgttcaagg cgatggctgc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat