NegoA.01013.a
2.3-bisphosphoglycerate-dependent phosphoglycerate mutase
| CENTER ID: | NegoA.01013.a |
| ORGANISM: | Neisseria gonorrhoeae NCCP11945 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I/II |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.01013.a.B1.GE39982 | full length | 1 | 227 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NegoA.01013.a.B1.PS38022 | full length | 1 | 227 |
Structures
5UM0
DEPOSITED: 1/25/2017
DETERMINATION: XRay
CLONE: NegoA.01013.a.B1.GE39982
PROTEIN: NegoA.01013.a.B1.PS38022
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|521006.8.peg.274 |
| UniProt: | B4RIY7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MELVFIRHGQ SEWNAKNLFT GWRDVKLSEQ GLAEAAAAGK KLKENGYEFD IAFTSVLTRA IKTCNIVLEE SDQLFVPQIK TWRLNERHYG RLQGLDKKQT AEKYGDEQVR IWRRSYDTLP PLLDKDDAFS AHKDRRYAHL PADVVPDGEN LKVTLERVLP FWEDQIAPAI LSGKRVLVAA HGNSLRALAK HIEGISDEDI MGLEIPTGQP LVYKLDDNLK VIEKFYL
NT Sequence
ATGGAATTGG TATTTATCCG CCACGGACAA AGCGAATGGA ACGCGAAAAA CCTGTTTACA GGCTGGCGCG ACGTGAAGCT GAGCGAGCAG GGGCTTGCCG AGGCCGCCGC CGCCGGTAAA AAACTGAAAG AAAACGGCTA TGAGTTCGAC ATCGCCTTCA CATCCGTCCT GACCCGCGCG ATTAAGACCT GCAACATCGT TTTGGAAGAA TCCGACCAAC TGTTCGTACC GCAAATCAAA ACATGGAGGC TGAACGAACG CCACTACGGC CGACTGCAAG GTTTGGACAA AAAACAAACC GCCGAAAAAT ACGGCGACGA GCAAGTCCGC ATCTGGCGGC GCAGCTACGA CACCCTGCCG CCGCTTTTGG ACAAAGACGA CGCGTTTTCC GCACACAAAG ACCGTCGCTA TGCCCATCTG CCTGCCGATG TCGTACCCGA CGGCGAAAAC CTGAAAGTAA CGCTGGAGCG CGTATTACCG TTTTGGGAAG ACCAAATCGC CCCCGCGATT TTGAGCGGCA AACGCGTCTT GGTAGCGGCG CACGGCAACT CCCTGCGCGC GCTGGCAAAA CACATCGAGG GCATTTCCGA CGAAGACATC ATGGGCTTGG AAATCCCGAC CGGCCAGCCG CTGGTGTACA AATTAGACGA CAACCTGAAA GTCATCGAGA AATTTTACCT GTAA
Details for NegoA.01013.a.B1.GE39982
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHME LVFIRHGQSE WNAKNLFTGW RDVKLSEQGL AEAAAAGKKL KENGYEFDIA FTSVLTRAIK TCNIVLEESD QLFVPQIKTW RLNERHYGRL QGLDKKQTAE KYGDEQVRIW RRSYDTLPPL LDKDDAFSAH KDRRYAHLPA DVVPDGENLK VTLERVLPFW EDQIAPAILS GKRVLVAAHG NSLRALAKHI EGISDEDIMG LEIPTGQPLV YKLDDNLKVI EKFYL
Validated NT Sequence
atcttactca cttacaggta aaatttctcg atgactttca ggttgtcgtc taatttgtac accagcggct ggccggtcgg gatttccaag cccatgatgt cttcgtcgga aatgccctcg atgtgttttg ccagcgcgcg cagggagttg ccgtgcgccg ctaccaagac gcgtttgccg ctcaaaatcg cgggggcgat ttggtcttcc caaaacggta atacgcgctc cagcgttact ttcaggtttt cgccgtcggg tacgacatcg gcaggcagat gggcatagcg acggtctttg tgtgcggaaa acgcgtcgtc tttgtccaaa agcggcggca gggtgtcgta gctgcgccgc cagatgcgga cttgctcgtc gccgtatttt tcggcggttt gttttttgtc caaaccttgc agtcggccgt agtggcgttc gttcagcctc catgttttga tttgcggtac gaacagttgg tcggattctt ccaaaacgat gttgcaggtc ttaatcgcgc gggtcaggac ggatgtgaag gcgatgtcga actcatagcc gttttctttc agttttttac cggcggcggc ggcctcggca agcccctgct cgctcagctt cacgtcgcgc cagcctgtaa acaggttttt cgcgttccat tcgctttgtc cgtggcggat aaataccaat tccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHME LVFIRHGQSE WNAKNLFTGW RDVKLSEQGL AEAAAAGKKL KENGYEFDIA FTSVLTRAIK TCNIVLEESD QLFVPQIKTW RLNERHYGRL QGLDKKQTAE KYGDEQVRIW RRSYDTLPPL LDKDDAFSAH KDRRYAHLPA DVVPDGENLK VTLERVLPFW EDQIAPAILS GKRVLVAAHG NSLRALAKHI EGISDEDIMG LEIPTGQPLV YKLDDNLKVI EKFYL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat tggtatttat ccgccacgga caaagcgaat ggaacgcgaa aaacctgttt acaggctggc gcgacgtgaa gctgagcgag caggggcttg ccgaggccgc cgccgccggt aaaaaactga aagaaaacgg ctatgagttc gacatcgcct tcacatccgt cctgacccgc gcgattaaga cctgcaacat cgttttggaa gaatccgacc aactgttcgt accgcaaatc aaaacatgga ggctgaacga acgccactac ggccgactgc aaggtttgga caaaaaacaa accgccgaaa aatacggcga cgagcaagtc cgcatctggc ggcgcagcta cgacaccctg ccgccgcttt tggacaaaga cgacgcgttt tccgcacaca aagaccgtcg ctatgcccat ctgcctgccg atgtcgtacc cgacggcgaa aacctgaaag taacgctgga gcgcgtatta ccgttttggg aagaccaaat cgcccccgcg attttgagcg gcaaacgcgt cttggtagcg gcgcacggca actccctgcg cgcgctggca aaacacatcg agggcatttc cgacgaagac atcatgggct tggaaatccc gaccggccag ccgctggtgt acaaattaga cgacaacctg aaagtcatcg agaaatttta cctgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for NegoA.01013.a.B1.PS38022
| PURIFICATION DATe: | 8/16/2016 |
| CONCENTRATION: | 14mg/ml |
| OBSERVED MW: | 23kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME LVFIRHGQSE WNAKNLFTGW RDVKLSEQGL AEAAAAGKKL KENGYEFDIA FTSVLTRAIK TCNIVLEESD QLFVPQIKTW RLNERHYGRL QGLDKKQTAE KYGDEQVRIW RRSYDTLPPL LDKDDAFSAH KDRRYAHLPA DVVPDGENLK VTLERVLPFW EDQIAPAILS GKRVLVAAHG NSLRALAKHI EGISDEDIMG LEIPTGQPLV YKLDDNLKVI EKFYL
Validated NT Sequence
atcttactca cttacaggta aaatttctcg atgactttca ggttgtcgtc taatttgtac accagcggct ggccggtcgg gatttccaag cccatgatgt cttcgtcgga aatgccctcg atgtgttttg ccagcgcgcg cagggagttg ccgtgcgccg ctaccaagac gcgtttgccg ctcaaaatcg cgggggcgat ttggtcttcc caaaacggta atacgcgctc cagcgttact ttcaggtttt cgccgtcggg tacgacatcg gcaggcagat gggcatagcg acggtctttg tgtgcggaaa acgcgtcgtc tttgtccaaa agcggcggca gggtgtcgta gctgcgccgc cagatgcgga cttgctcgtc gccgtatttt tcggcggttt gttttttgtc caaaccttgc agtcggccgt agtggcgttc gttcagcctc catgttttga tttgcggtac gaacagttgg tcggattctt ccaaaacgat gttgcaggtc ttaatcgcgc gggtcaggac ggatgtgaag gcgatgtcga actcatagcc gttttctttc agttttttac cggcggcggc ggcctcggca agcccctgct cgctcagctt cacgtcgcgc cagcctgtaa acaggttttt cgcgttccat tcgctttgtc cgtggcggat aaataccaat tccatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHME LVFIRHGQSE WNAKNLFTGW RDVKLSEQGL AEAAAAGKKL KENGYEFDIA FTSVLTRAIK TCNIVLEESD QLFVPQIKTW RLNERHYGRL QGLDKKQTAE KYGDEQVRIW RRSYDTLPPL LDKDDAFSAH KDRRYAHLPA DVVPDGENLK VTLERVLPFW EDQIAPAILS GKRVLVAAHG NSLRALAKHI EGISDEDIMG LEIPTGQPLV YKLDDNLKVI EKFYL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAT TGGTATTTAT CCGCCACGGA CAAAGCGAAT GGAACGCGAA AAACCTGTTT ACAGGCTGGC GCGACGTGAA GCTGAGCGAG CAGGGGCTTG CCGAGGCCGC CGCCGCCGGT AAAAAACTGA AAGAAAACGG CTATGAGTTC GACATCGCCT TCACATCCGT CCTGACCCGC GCGATTAAGA CCTGCAACAT CGTTTTGGAA GAATCCGACC AACTGTTCGT ACCGCAAATC AAAACATGGA GGCTGAACGA ACGCCACTAC GGCCGACTGC AAGGTTTGGA CAAAAAACAA ACCGCCGAAA AATACGGCGA CGAGCAAGTC CGCATCTGGC GGCGCAGCTA CGACACCCTG CCGCCGCTTT TGGACAAAGA CGACGCGTTT TCCGCACACA AAGACCGTCG CTATGCCCAT CTGCCTGCCG ATGTCGTACC CGACGGCGAA AACCTGAAAG TAACGCTGGA GCGCGTATTA CCGTTTTGGG AAGACCAAAT CGCCCCCGCG ATTTTGAGCG GCAAACGCGT CTTGGTAGCG GCGCACGGCA ACTCCCTGCG CGCGCTGGCA AAACACATCG AGGGCATTTC CGACGAAGAC ATCATGGGCT TGGAAATCCC GACCGGCCAG CCGCTGGTGT ACAAATTAGA CGACAACCTG AAAGTCATCG AGAAATTTTA CCTGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT